Introduction: Recent technological developments have enabled many scientists to produce a wide variety of foods that meet consumers’ desires for diversity and quality. Genetically modified (GM) foods have long been a...Introduction: Recent technological developments have enabled many scientists to produce a wide variety of foods that meet consumers’ desires for diversity and quality. Genetically modified (GM) foods have long been a controversial topic, and consumer reliance on these foods depends on their trust in biotechnology institutions due to their lack of knowledge about the risks and benefits. This study aimed to assess the awareness, attitude, and perception of GM foods among the Saudi population, as well as to identify the association between age, gender, and education level in the studied population and their awareness level towards genetically modified foods. Method: Data was collected using a structured, pretested questionnaire drawn from previous studies, after obtaining written consent. The questionnaire included three sections focusing on personal data, general knowledge of GM foods, and perception, attitudes, and beliefs towards GM foods. Our study included 500 responses. Results: The majority of the participants were female, located in the central region, and had a university education. Most participants provided a correct definition of GM foods and indicated that they had heard about them, but were unsure if they had eaten these foods in the past. Their knowledge of these foods was good. Regarding opinions and attitudes towards these foods, the majority of participants were neutral. They approved of using GM foods because it produces more food, tastes better, and can be stored for a long time. In conclusion, the study highlights the urgent necessity to raise awareness and knowledge regarding genetically modified foods among the Saudi population. It emphasizes the importance of educating consumers about the potential advantages and risks associated with consuming such foods, enabling them to make informed decisions. It is crucial to integrate awareness and education about genetically modified foods into the technological and agricultural advancements in Saudi Arabia.展开更多
The commercial cultivation of genetically modified(GM)crops has eased the global food crisis and brought considerable economic and social benefits to countries.Because of the potential safety problems,it is necessary ...The commercial cultivation of genetically modified(GM)crops has eased the global food crisis and brought considerable economic and social benefits to countries.Because of the potential safety problems,it is necessary to make clear the molecular genetic characteristics,edible safety,planting,processing,and other aspects of the safety evaluation of GM crops.The safety problems existing in the cultivation of GM crops,safety evaluation and detection of GM crops were introduced in this paper,which provided the basis for safety evaluation and effective supervision of GM crops and their products.Commercial cultivation and reasonable supervision based on safety evaluation have far-reaching significance for ensuring consumer safety,enhancing the credibility of the national political system and enhancing citizens'confidence in the safety of GM crop products for consumption.展开更多
With the research on and development of Genetically Modified Food (GMF), people's attitude toward GMF may fall into two divergent categories, typical- ly represented by the United States(US) and the European Unio...With the research on and development of Genetically Modified Food (GMF), people's attitude toward GMF may fall into two divergent categories, typical- ly represented by the United States(US) and the European Union(EU), respectively. The former follows a "sound science principle" and firmly objects to the precautionary principle, namely a permissive policy of positive support of and voluntary labelling on GMF; while the latter adopts a cautiously precautionary principle, requiring mandatory labelling and traceability. From the standpoint of regulatory principles, together with corresponding supervisory measures and relevant provisions, this paper compared the execution of directives and provisions on GMF from the initial policies enacted by the US and EU to current situation combined with the track and analysis of latest polic ies issued.展开更多
In order to have knowledge about the college students'awareness and acceptance of genetically modified foods and their attitudes toward the identification of genetically modified foods and the government's reg...In order to have knowledge about the college students'awareness and acceptance of genetically modified foods and their attitudes toward the identification of genetically modified foods and the government's regulation,we conduct a questionnaire survey of 150 college students'cognition of genetically modified foods in North College Town of Zhengzhou City.The results show that the college students have a certain understanding of genetically modified foods,but the cognition level is low;electronic media has become the main channel for the current college students to know the information about genetically modified foods;for security reasons,the majority of college students are wary of genetically modified foods,and pay more attention to whether there is genetically modified component in the foods labeling;college students generally believe that the government should strengthen the supervision of genetically modified foods,and make the GM labeling system strict,to protect consumers'right to know and choice.展开更多
The production of foods with genetically modified organisms (GMOs) has risen rapidly over the past three decades to comprise nearly 90% of crops grown in the United States today. Currently, there are no mandates for l...The production of foods with genetically modified organisms (GMOs) has risen rapidly over the past three decades to comprise nearly 90% of crops grown in the United States today. Currently, there are no mandates for labeling foods containing GMOs. GMO agricultural crops contain the insertion of genes encoding for pesticides, pesticide resistance, growth factors, or other substances not normally present. In addition to the foreign genes that are inserted, hundreds to thousands of mutations disrupt normal genes in GMO plants. Recently, animal studies have demonstrated toxicity of GMO foods causing organ failure, infertility, carcinomas and death. The FDA requirement of ingredients added to foods be labeled on the product is not applied to GMO foods, precluding the consumer’s right to know. GMOs provide an economic incentive to companies because the seeds can be patented, driving up costs and creating the potential for monopolies. Herbicide-resistance conferred by GMOs has resulted in higher pesticide applications, which correlate with higher human cancer rates, and the emergence of pesticide-resistant weeds and insects. GMO toxins are spreading into to non-target insects, waterways and aquatic organisms, with toxicity to non-target organisms and resultant contamination of disparate ecosystems in the food chain. The appropriateness of mandatory GMO labeling of foods in the United States is discussed.展开更多
Having a clear understanding of consumers' awareness of genetically modified food and their willingness to buy, plays a very important role in formulating the regulatory policy of genetically modified food and reg...Having a clear understanding of consumers' awareness of genetically modified food and their willingness to buy, plays a very important role in formulating the regulatory policy of genetically modified food and regulating the market of genetically modified food. This paper takes the supermarket consumers as the study object. Through on-site questionnaire survey, we find that consumers' awareness of genetically modified food is not high in Yanbian Prefecture, and their willingness to buy is also low; the prices of genetically modified food, consumers' income, educational level and so on, are the main factors that affect the willingness to buy. Based on this, we put forth the relevant recommendations: increasing publicity efforts to safeguard consumers' right to know and choose; increasing supervision efforts to improve the existing regulatory system of genetically modified food; actively organizing forces to carry out the study on safety of genetically modified food.展开更多
The debate about the safety of genetically modified foods has never stopped,and different consumers have different judgments. On the basis of literature research,this paper designs the corresponding questionnaire for ...The debate about the safety of genetically modified foods has never stopped,and different consumers have different judgments. On the basis of literature research,this paper designs the corresponding questionnaire for empirical analysis. With the college students as the object of study,this paper explores the differences in perceived genetically modified food risk by the college students with different majors,as well as the differences in the information processing mode adopted by the college students with different majors,and the differences in the perceived risk after adopting different information processing mode. The results show that there are significant differences in the perceived genetically modified food risk among the college students with different majors,the economics students have the highest average perceived risk; there are also significant differences in the information processing mode adopted by the college students with different majors,and the perceived risk is different when using the heuristic information processing mode.展开更多
In recent years, food security and safety have attracted increasing attention due to the worldwide research and development of genetically modified (GM) rice, and the controversy over the commercialization of GM ric...In recent years, food security and safety have attracted increasing attention due to the worldwide research and development of genetically modified (GM) rice, and the controversy over the commercialization of GM rice. And the identification of GM rice is of great significance. Therefore, in the present study, the po- tential problems in the identification of GM rice with PCR were analyzed both at a technical level and from a theoretical perspective. In addition, PCR detection on the transgenic elements: promoter, terminator, internal reference gene and target gene was discussed, respectively. The possible solutions were proposed based on the principles of plant virology and genetic engineering.展开更多
Objective: To expose the likely impact of genetically modified foods in Collective Feeding. Methods: This was a case study conducted on a steel company (SC) in Rio de Janeiro State. The likely impact recognition began...Objective: To expose the likely impact of genetically modified foods in Collective Feeding. Methods: This was a case study conducted on a steel company (SC) in Rio de Janeiro State. The likely impact recognition began with the preparation of a soy food products’ list. Then, we verified these products’ frequency on the Food and Nutrition Unit (FNU) menu from the SC, outlining the offer profile. We calculated odds ratios and binomial distribution probability for assessing exposure to soy that may be transgenic. Food frequency questionnaire (FFQ) regarding soy products was applied. The convergence of daily products’ relative frequency of this population and the population from a reference study about FFQ validation in Rio de Janeiro was verified. Confrontation took place between the classification of foods by the consumers’ likely exposure to soy and the percentage deviation between observed relative frequency of products’ intake offered on the FNU and on the reference study. Results: A list of 51 products were created and 16 of them integrated the menu, e.g. flour, chicken, sausage, maize, instant pudding, bologna, hot dogs and peanuts. Food usually consumed by FNU users and concomitantly consumed in reference study were cookies, flour, spaghetti, bread, cake, instant pudding, sausage and hot dogs. By the classification and percentage deviations results, cookies, breads and pasta were the “villains” of soy’s susceptibility. Coclusion: It was possible to show the likely impact of genetically modified foods in Collective Feeding, confirming that these consumers were exposed to soybeans that may be transgenic.展开更多
[Objective] This study aimed to evaluate tbe healthy risk of genetically modified ( GM ) soybeans by using a novel approach for functions and safety of food. [ Me^od] Different from traditional evaluation of substan...[Objective] This study aimed to evaluate tbe healthy risk of genetically modified ( GM ) soybeans by using a novel approach for functions and safety of food. [ Me^od] Different from traditional evaluation of substantial equivalence, three great innovations were performed in this study, involving in basic diet, evalu- ation approaches and principle, as well as the clarification of connotation differences between absolute and relative mass of organs. Hence a novel BDI-GS (Bendib Damage Index and General Score) evaluation approach was established and applied in comparative evaluation between RR GM and natural soybeans. Healthy male ICR mice during linear growth were selected; experimental mice were fed with 15% RR GM soybeans and 15% natural soybeans blending maize meal diets, and control mice were fed with single maize meal diet for 13 d; the mice were dissected after collecting blood samples and perfectly obtained nine organs or tissues to re- cord their masses and conduct statistical analyses. [Result] Plenty of matching information was obtained through simple design. The growth performance of treated mice was markedly of individual differences, some mice were thwarted due to regular intake of RR soybeans. Meanwhile, the functions and safety of RR soybeans were markedly lowered in overall nutritional and healthy effects than those of natural soybeans expressed in GS values, and presents some declines in nutrition and health of thymus, pancreas and spermary; especially, it can make thymus immune (P 〈0.05) in markedly lower level than that of natural soybeans. [ Conclusion] Therefore, major troubles and risks of RR soybeans intake are of personal risks in different degrees, in addition, it may increase sub-health and related chronic epi- demics risks, and herein it will presents certain safety issues. The creation of this novel evaluation system provides a simple and available evaluation approach for functions and potential risks revelation of food effects, and will yield far-reaching influences to safety evaluation and healthy development of GM foods, as well as public health.展开更多
Ability to modify plants at the genomic level by advanced molecular technology has enhanced the scope of improvements in plant traits attempted earlier through conventional breeding methods. Techniques such as genetic...Ability to modify plants at the genomic level by advanced molecular technology has enhanced the scope of improvements in plant traits attempted earlier through conventional breeding methods. Techniques such as genetic transformation have opened new vistas whereby functional genes, not commonly present in a particular species can be added from other species. The traits incorporated into the genetically engineered plants in the beginning were confined to those governed by dominant genes, e.g. insecticide resistance and herbicide tolerance but advancements with time now also permit the transfer of complexly inherited traits such as drought and cold tolerance. Transgenic technology is also useful in understanding gene expression and metabolic pathways which can then be used to harness the full genomic potential of the plant. This review presents a narrative on development of transgenics and their use for the improvement of field, industrial and pharmaceuticals crops. In addition, discussions are made on current status on genetically modified crops, hurdles to genetic engineering, overcoming strategies and future scope.展开更多
In order to accurately identify the first and second generations of epsps genetically modified(GM) soybeans and related products,a broad spectrum identification approach was established using the real time Polymerase ...In order to accurately identify the first and second generations of epsps genetically modified(GM) soybeans and related products,a broad spectrum identification approach was established using the real time Polymerase Chain Reaction(PCR) principle according to the homology of epsps genes of the first and second generations of GM soybeans.A pair of primer and probe was designed to simultaneously identify exogenous gene epsps of two generations of GM soybeans.Besides,evaluation was carried out on this approach from the accuracy,specificity,sensitivity and reproducibility.The experimental results indicated that(ⅰ) although there is certain difference in epsps gene sequence between the first and second generations of epsps genetically modified(GM) soybeans,the established approach can simultaneously detect the epsps genes of the bean curd using two generations of soybean as raw materials;(ⅱ) in the accuracy and specificity experiment,only cp4-epsps genes of two generations of GM soybeans were detected,so this approach has high specificity and accuracy;(ⅲ) in the experiment of 5 copies of epsps genes of 40 repeated identification reaction systems,5 copies of epsps genes can be detected each time,therefore at 100% confidence level,this approach can identify 5 copies of epsps genes,showing that this approach has high sensitivity and reproducibility.展开更多
文摘Introduction: Recent technological developments have enabled many scientists to produce a wide variety of foods that meet consumers’ desires for diversity and quality. Genetically modified (GM) foods have long been a controversial topic, and consumer reliance on these foods depends on their trust in biotechnology institutions due to their lack of knowledge about the risks and benefits. This study aimed to assess the awareness, attitude, and perception of GM foods among the Saudi population, as well as to identify the association between age, gender, and education level in the studied population and their awareness level towards genetically modified foods. Method: Data was collected using a structured, pretested questionnaire drawn from previous studies, after obtaining written consent. The questionnaire included three sections focusing on personal data, general knowledge of GM foods, and perception, attitudes, and beliefs towards GM foods. Our study included 500 responses. Results: The majority of the participants were female, located in the central region, and had a university education. Most participants provided a correct definition of GM foods and indicated that they had heard about them, but were unsure if they had eaten these foods in the past. Their knowledge of these foods was good. Regarding opinions and attitudes towards these foods, the majority of participants were neutral. They approved of using GM foods because it produces more food, tastes better, and can be stored for a long time. In conclusion, the study highlights the urgent necessity to raise awareness and knowledge regarding genetically modified foods among the Saudi population. It emphasizes the importance of educating consumers about the potential advantages and risks associated with consuming such foods, enabling them to make informed decisions. It is crucial to integrate awareness and education about genetically modified foods into the technological and agricultural advancements in Saudi Arabia.
基金Supported by the China Postdoctoral Science Foundation(2023M730312)the Science and Technology Plan Projects of the State Administration for Market Regulation(2022MK002)the National Key Research and Development Program(2022YFF0606105)。
文摘The commercial cultivation of genetically modified(GM)crops has eased the global food crisis and brought considerable economic and social benefits to countries.Because of the potential safety problems,it is necessary to make clear the molecular genetic characteristics,edible safety,planting,processing,and other aspects of the safety evaluation of GM crops.The safety problems existing in the cultivation of GM crops,safety evaluation and detection of GM crops were introduced in this paper,which provided the basis for safety evaluation and effective supervision of GM crops and their products.Commercial cultivation and reasonable supervision based on safety evaluation have far-reaching significance for ensuring consumer safety,enhancing the credibility of the national political system and enhancing citizens'confidence in the safety of GM crop products for consumption.
文摘With the research on and development of Genetically Modified Food (GMF), people's attitude toward GMF may fall into two divergent categories, typical- ly represented by the United States(US) and the European Union(EU), respectively. The former follows a "sound science principle" and firmly objects to the precautionary principle, namely a permissive policy of positive support of and voluntary labelling on GMF; while the latter adopts a cautiously precautionary principle, requiring mandatory labelling and traceability. From the standpoint of regulatory principles, together with corresponding supervisory measures and relevant provisions, this paper compared the execution of directives and provisions on GMF from the initial policies enacted by the US and EU to current situation combined with the track and analysis of latest polic ies issued.
基金Supported by Henan Provincial Government's Decision-making Research and Bid Invitation Project(2012B763)
文摘In order to have knowledge about the college students'awareness and acceptance of genetically modified foods and their attitudes toward the identification of genetically modified foods and the government's regulation,we conduct a questionnaire survey of 150 college students'cognition of genetically modified foods in North College Town of Zhengzhou City.The results show that the college students have a certain understanding of genetically modified foods,but the cognition level is low;electronic media has become the main channel for the current college students to know the information about genetically modified foods;for security reasons,the majority of college students are wary of genetically modified foods,and pay more attention to whether there is genetically modified component in the foods labeling;college students generally believe that the government should strengthen the supervision of genetically modified foods,and make the GM labeling system strict,to protect consumers'right to know and choice.
文摘The production of foods with genetically modified organisms (GMOs) has risen rapidly over the past three decades to comprise nearly 90% of crops grown in the United States today. Currently, there are no mandates for labeling foods containing GMOs. GMO agricultural crops contain the insertion of genes encoding for pesticides, pesticide resistance, growth factors, or other substances not normally present. In addition to the foreign genes that are inserted, hundreds to thousands of mutations disrupt normal genes in GMO plants. Recently, animal studies have demonstrated toxicity of GMO foods causing organ failure, infertility, carcinomas and death. The FDA requirement of ingredients added to foods be labeled on the product is not applied to GMO foods, precluding the consumer’s right to know. GMOs provide an economic incentive to companies because the seeds can be patented, driving up costs and creating the potential for monopolies. Herbicide-resistance conferred by GMOs has resulted in higher pesticide applications, which correlate with higher human cancer rates, and the emergence of pesticide-resistant weeds and insects. GMO toxins are spreading into to non-target insects, waterways and aquatic organisms, with toxicity to non-target organisms and resultant contamination of disparate ecosystems in the food chain. The appropriateness of mandatory GMO labeling of foods in the United States is discussed.
基金Supported by Jilin Social Science Fund Project (2008Bjjx16)National Natural Science Foundation Project (70963014)
文摘Having a clear understanding of consumers' awareness of genetically modified food and their willingness to buy, plays a very important role in formulating the regulatory policy of genetically modified food and regulating the market of genetically modified food. This paper takes the supermarket consumers as the study object. Through on-site questionnaire survey, we find that consumers' awareness of genetically modified food is not high in Yanbian Prefecture, and their willingness to buy is also low; the prices of genetically modified food, consumers' income, educational level and so on, are the main factors that affect the willingness to buy. Based on this, we put forth the relevant recommendations: increasing publicity efforts to safeguard consumers' right to know and choose; increasing supervision efforts to improve the existing regulatory system of genetically modified food; actively organizing forces to carry out the study on safety of genetically modified food.
文摘The debate about the safety of genetically modified foods has never stopped,and different consumers have different judgments. On the basis of literature research,this paper designs the corresponding questionnaire for empirical analysis. With the college students as the object of study,this paper explores the differences in perceived genetically modified food risk by the college students with different majors,as well as the differences in the information processing mode adopted by the college students with different majors,and the differences in the perceived risk after adopting different information processing mode. The results show that there are significant differences in the perceived genetically modified food risk among the college students with different majors,the economics students have the highest average perceived risk; there are also significant differences in the information processing mode adopted by the college students with different majors,and the perceived risk is different when using the heuristic information processing mode.
基金Supported by Key Special Project for Breeding and Cultivation of GMO Varieties(2011ZX08001-001,2014ZX0800101B)Special Fund from the Department of Finance of Hubei Province(2011-2015)Collaborative Breeding Project for Rice(2013-2017)
文摘In recent years, food security and safety have attracted increasing attention due to the worldwide research and development of genetically modified (GM) rice, and the controversy over the commercialization of GM rice. And the identification of GM rice is of great significance. Therefore, in the present study, the po- tential problems in the identification of GM rice with PCR were analyzed both at a technical level and from a theoretical perspective. In addition, PCR detection on the transgenic elements: promoter, terminator, internal reference gene and target gene was discussed, respectively. The possible solutions were proposed based on the principles of plant virology and genetic engineering.
文摘Objective: To expose the likely impact of genetically modified foods in Collective Feeding. Methods: This was a case study conducted on a steel company (SC) in Rio de Janeiro State. The likely impact recognition began with the preparation of a soy food products’ list. Then, we verified these products’ frequency on the Food and Nutrition Unit (FNU) menu from the SC, outlining the offer profile. We calculated odds ratios and binomial distribution probability for assessing exposure to soy that may be transgenic. Food frequency questionnaire (FFQ) regarding soy products was applied. The convergence of daily products’ relative frequency of this population and the population from a reference study about FFQ validation in Rio de Janeiro was verified. Confrontation took place between the classification of foods by the consumers’ likely exposure to soy and the percentage deviation between observed relative frequency of products’ intake offered on the FNU and on the reference study. Results: A list of 51 products were created and 16 of them integrated the menu, e.g. flour, chicken, sausage, maize, instant pudding, bologna, hot dogs and peanuts. Food usually consumed by FNU users and concomitantly consumed in reference study were cookies, flour, spaghetti, bread, cake, instant pudding, sausage and hot dogs. By the classification and percentage deviations results, cookies, breads and pasta were the “villains” of soy’s susceptibility. Coclusion: It was possible to show the likely impact of genetically modified foods in Collective Feeding, confirming that these consumers were exposed to soybeans that may be transgenic.
基金Supported by Development Fund of the Institute of Radiation Medicine(No.SF1227)Research Fund for Youth Scholars of Union Medical College(No.2012D03)Research Fund for the Doctoral Program of Higher Education of China(No.20121106120042)
文摘[Objective] This study aimed to evaluate tbe healthy risk of genetically modified ( GM ) soybeans by using a novel approach for functions and safety of food. [ Me^od] Different from traditional evaluation of substantial equivalence, three great innovations were performed in this study, involving in basic diet, evalu- ation approaches and principle, as well as the clarification of connotation differences between absolute and relative mass of organs. Hence a novel BDI-GS (Bendib Damage Index and General Score) evaluation approach was established and applied in comparative evaluation between RR GM and natural soybeans. Healthy male ICR mice during linear growth were selected; experimental mice were fed with 15% RR GM soybeans and 15% natural soybeans blending maize meal diets, and control mice were fed with single maize meal diet for 13 d; the mice were dissected after collecting blood samples and perfectly obtained nine organs or tissues to re- cord their masses and conduct statistical analyses. [Result] Plenty of matching information was obtained through simple design. The growth performance of treated mice was markedly of individual differences, some mice were thwarted due to regular intake of RR soybeans. Meanwhile, the functions and safety of RR soybeans were markedly lowered in overall nutritional and healthy effects than those of natural soybeans expressed in GS values, and presents some declines in nutrition and health of thymus, pancreas and spermary; especially, it can make thymus immune (P 〈0.05) in markedly lower level than that of natural soybeans. [ Conclusion] Therefore, major troubles and risks of RR soybeans intake are of personal risks in different degrees, in addition, it may increase sub-health and related chronic epi- demics risks, and herein it will presents certain safety issues. The creation of this novel evaluation system provides a simple and available evaluation approach for functions and potential risks revelation of food effects, and will yield far-reaching influences to safety evaluation and healthy development of GM foods, as well as public health.
文摘Ability to modify plants at the genomic level by advanced molecular technology has enhanced the scope of improvements in plant traits attempted earlier through conventional breeding methods. Techniques such as genetic transformation have opened new vistas whereby functional genes, not commonly present in a particular species can be added from other species. The traits incorporated into the genetically engineered plants in the beginning were confined to those governed by dominant genes, e.g. insecticide resistance and herbicide tolerance but advancements with time now also permit the transfer of complexly inherited traits such as drought and cold tolerance. Transgenic technology is also useful in understanding gene expression and metabolic pathways which can then be used to harness the full genomic potential of the plant. This review presents a narrative on development of transgenics and their use for the improvement of field, industrial and pharmaceuticals crops. In addition, discussions are made on current status on genetically modified crops, hurdles to genetic engineering, overcoming strategies and future scope.
基金Supported by Project for Promotion of Financial Innovation Ability of Sichuan Province(2016GXTZ-010)Dongrun-Yau Science Award(Biology,2016)
文摘In order to accurately identify the first and second generations of epsps genetically modified(GM) soybeans and related products,a broad spectrum identification approach was established using the real time Polymerase Chain Reaction(PCR) principle according to the homology of epsps genes of the first and second generations of GM soybeans.A pair of primer and probe was designed to simultaneously identify exogenous gene epsps of two generations of GM soybeans.Besides,evaluation was carried out on this approach from the accuracy,specificity,sensitivity and reproducibility.The experimental results indicated that(ⅰ) although there is certain difference in epsps gene sequence between the first and second generations of epsps genetically modified(GM) soybeans,the established approach can simultaneously detect the epsps genes of the bean curd using two generations of soybean as raw materials;(ⅱ) in the accuracy and specificity experiment,only cp4-epsps genes of two generations of GM soybeans were detected,so this approach has high specificity and accuracy;(ⅲ) in the experiment of 5 copies of epsps genes of 40 repeated identification reaction systems,5 copies of epsps genes can be detected each time,therefore at 100% confidence level,this approach can identify 5 copies of epsps genes,showing that this approach has high sensitivity and reproducibility.