从2001年WHO/IUATLD全球抗结核药物耐药监测之内蒙古耐药监测结核菌1 114株中,选取经比例法药敏结果得到的耐多药(MDR)结核菌188株,利用Geno Type MTBDRplus方法检测该批菌株的RIF、INH耐药情况及其耐药基因突变形式。最终得到MDR菌株10...从2001年WHO/IUATLD全球抗结核药物耐药监测之内蒙古耐药监测结核菌1 114株中,选取经比例法药敏结果得到的耐多药(MDR)结核菌188株,利用Geno Type MTBDRplus方法检测该批菌株的RIF、INH耐药情况及其耐药基因突变形式。最终得到MDR菌株103(54.79%)株,单耐RIF菌株52(27.66%)株,单耐INH菌株10(5.32%)株,全敏感菌株20株,TUB条带缺失1株,kat G质控带缺失2株。RIF耐药菌株检测的是rpo B基因区,该基因突变菌株有155株;rpo B S531L突变菌株为49.68%(77/155)。INH耐药菌株检测的是kat G基因区和inh A基因启动子区,共113株,其中kat G基因突变菌株占79.65%(90/113),主要是S3-15T1突变;inh A基因突变菌株占22.12%(25/113)。因此,Geno Type MTBDRplus可用于内蒙古地区MDR结核菌的快速检测。其中rpo BS531L突变形式在RIF耐药菌株中最常见;在INH耐药菌株中,kat G基因突变较inh A基因突变常见,S315T1突变是INH耐药菌中常见的突变形式。展开更多
Chronic active hepatitis(CAH) is acknowledged as an imperative risk factor for the development of liver injury and hepatocellular carcinoma.The histological end points of CAH are chronic inflammation,fibrosis and cirr...Chronic active hepatitis(CAH) is acknowledged as an imperative risk factor for the development of liver injury and hepatocellular carcinoma.The histological end points of CAH are chronic inflammation,fibrosis and cirrhosis which are coupled with increased DNA synthesis in cirrhotic vs healthy normal livers.The potential mechanism involved in CAH includes a combination of processes leading to liver cell necrosis,inflammation and cytokine production and liver scaring(fibrosis).The severity of liver damage is regulated by Hepatitis B virus genotypes and viral components.The viral and cellular factors that contribute to liver injury are discussed in this article.Liver injury caused by the viral infection affects many cellular processes such as cell signaling,apoptosis,transcription,DNA repair which in turn induce radical effects on cell survival,growth,transformation and maintenance.The consequence of such perturbations is resulted in the alteration of bile secretion,gluconeogenesis,glycolysis,detoxification and metabolism of carbohydrates,proteins,fat and balance of nutrients.The identification and elucidation of the molecular pathways perturbed by the viral proteins are important in order to design effective strategy to minimize and/or restore the hepatocytes injury.展开更多
目的：探讨线性探针技术（Geno Type MTBDRplus试剂盒）在复治涂阳肺结核患者中的应用价值。方法：收集2013年4月-2015年10月期间的122例复治涂阳肺结核患者的痰液标本进行线性探针技术检测，并同时采用绝对浓度法（罗氏培养基法）进行...目的：探讨线性探针技术（Geno Type MTBDRplus试剂盒）在复治涂阳肺结核患者中的应用价值。方法：收集2013年4月-2015年10月期间的122例复治涂阳肺结核患者的痰液标本进行线性探针技术检测，并同时采用绝对浓度法（罗氏培养基法）进行结核药敏试验。以药敏试验结果为参照，计算线性探针技术在检测异烟肼和利福平耐药方面的敏感性与特异性、阳性似然比、阴性似然比和诊断比值比。结果：患者年龄为48．0±18．8岁，75．铂患者为男性。在检测异烟肼耐药性方面，其敏感性、特异性、阳性似然比、阴性似然比和诊断比值比分别为85．1％（72．3%-92．6%）、86．7％（77．2％-92．6%）、6．38（3．54～11．51）、0．17（0．09-0．34）和37．1（13．1-105．4）；在检测利福平耐药性方面，分别为88．7%（77．4％-94．7％）、89．9％（80．5％95．0%）、8．74（4．30%-17．76）、0．13（0．06-0．27）和69．4（21．9-220．1）。在诊断多重耐药结核方面，分别为81．0%（66．7%-90．0%）、87．5％（78．5％-93．1％）、6．48（3．56—11．78）、0．22（0．12～0．41）和29．8（10．77-82．17）。结论：线性探针技术在诊断复治涂阳肺结核患者的异烟肼与利福平耐药性方面具有较好的敏感性与特异性，值得进一步推广。展开更多
文摘Chronic active hepatitis(CAH) is acknowledged as an imperative risk factor for the development of liver injury and hepatocellular carcinoma.The histological end points of CAH are chronic inflammation,fibrosis and cirrhosis which are coupled with increased DNA synthesis in cirrhotic vs healthy normal livers.The potential mechanism involved in CAH includes a combination of processes leading to liver cell necrosis,inflammation and cytokine production and liver scaring(fibrosis).The severity of liver damage is regulated by Hepatitis B virus genotypes and viral components.The viral and cellular factors that contribute to liver injury are discussed in this article.Liver injury caused by the viral infection affects many cellular processes such as cell signaling,apoptosis,transcription,DNA repair which in turn induce radical effects on cell survival,growth,transformation and maintenance.The consequence of such perturbations is resulted in the alteration of bile secretion,gluconeogenesis,glycolysis,detoxification and metabolism of carbohydrates,proteins,fat and balance of nutrients.The identification and elucidation of the molecular pathways perturbed by the viral proteins are important in order to design effective strategy to minimize and/or restore the hepatocytes injury.
文摘目的：探讨线性探针技术（Geno Type MTBDRplus试剂盒）在复治涂阳肺结核患者中的应用价值。方法：收集2013年4月-2015年10月期间的122例复治涂阳肺结核患者的痰液标本进行线性探针技术检测，并同时采用绝对浓度法（罗氏培养基法）进行结核药敏试验。以药敏试验结果为参照，计算线性探针技术在检测异烟肼和利福平耐药方面的敏感性与特异性、阳性似然比、阴性似然比和诊断比值比。结果：患者年龄为48．0±18．8岁，75．铂患者为男性。在检测异烟肼耐药性方面，其敏感性、特异性、阳性似然比、阴性似然比和诊断比值比分别为85．1％（72．3%-92．6%）、86．7％（77．2％-92．6%）、6．38（3．54～11．51）、0．17（0．09-0．34）和37．1（13．1-105．4）；在检测利福平耐药性方面，分别为88．7%（77．4％-94．7％）、89．9％（80．5％95．0%）、8．74（4．30%-17．76）、0．13（0．06-0．27）和69．4（21．9-220．1）。在诊断多重耐药结核方面，分别为81．0%（66．7%-90．0%）、87．5％（78．5％-93．1％）、6．48（3．56—11．78）、0．22（0．12～0．41）和29．8（10．77-82．17）。结论：线性探针技术在诊断复治涂阳肺结核患者的异烟肼与利福平耐药性方面具有较好的敏感性与特异性，值得进一步推广。
