A few individuals with intermediate morphology always appeared in the sympatric distributions of Gentiana straminea and G.siphonantha.These intermediate individuals were hypothesized to be the hybrids of two species a...A few individuals with intermediate morphology always appeared in the sympatric distributions of Gentiana straminea and G.siphonantha.These intermediate individuals were hypothesized to be the hybrids of two species after a careful evaluation of their morphological characteristics.To test this hypothesis,sequence comparison of the internal transcribed spacer(ITS)regions of the nuclear ribosomal and trnS(GCU)-trnG(UCC)intergenic spacer region of the chloroplast DNA from Gentiana straminea,G.siphonantha and the putative hybrids was performed.The results suggest that most intermediate individuals were the natural hybrids between G.straminea and G.siphonantha.In addition,we examined the sequence variation among the individuals of both parent species and analyzed the possibility leading to the incongruent identification in some individuals based on morphologic and molecular evidences,respectively.The intraspecific diversification of DNA fragments within both parent species and their high variability in hybrid swarms probably resulted from chloroplast genome recombination and incomplete lineage sorting during the early stages of speciation origin of the parent species.展开更多
利用高效液相色谱法建立了青海秦艽的色谱指纹图谱.色谱条件:K rom as il C18柱(250 mm×4.6 mm×5μm),甲醇∶磷酸水(0.04%)梯度洗脱,流速为1 mL/m in,检测波长222 nm.通过比较发现秦艽样品的13个主要共有峰,可作为鉴别秦艽药...利用高效液相色谱法建立了青海秦艽的色谱指纹图谱.色谱条件:K rom as il C18柱(250 mm×4.6 mm×5μm),甲醇∶磷酸水(0.04%)梯度洗脱,流速为1 mL/m in,检测波长222 nm.通过比较发现秦艽样品的13个主要共有峰,可作为鉴别秦艽药材的主要依据.对12批秦艽药材进行了相似度计算和聚类分析,结果表明,所建立的秦艽指纹图谱可用于秦艽药材的真伪鉴别和质量评价.展开更多
基金The project was supported by the National Natural Science Foundation of China(Grant No.30572329).
文摘A few individuals with intermediate morphology always appeared in the sympatric distributions of Gentiana straminea and G.siphonantha.These intermediate individuals were hypothesized to be the hybrids of two species after a careful evaluation of their morphological characteristics.To test this hypothesis,sequence comparison of the internal transcribed spacer(ITS)regions of the nuclear ribosomal and trnS(GCU)-trnG(UCC)intergenic spacer region of the chloroplast DNA from Gentiana straminea,G.siphonantha and the putative hybrids was performed.The results suggest that most intermediate individuals were the natural hybrids between G.straminea and G.siphonantha.In addition,we examined the sequence variation among the individuals of both parent species and analyzed the possibility leading to the incongruent identification in some individuals based on morphologic and molecular evidences,respectively.The intraspecific diversification of DNA fragments within both parent species and their high variability in hybrid swarms probably resulted from chloroplast genome recombination and incomplete lineage sorting during the early stages of speciation origin of the parent species.
文摘利用高效液相色谱法建立了青海秦艽的色谱指纹图谱.色谱条件:K rom as il C18柱(250 mm×4.6 mm×5μm),甲醇∶磷酸水(0.04%)梯度洗脱,流速为1 mL/m in,检测波长222 nm.通过比较发现秦艽样品的13个主要共有峰,可作为鉴别秦艽药材的主要依据.对12批秦艽药材进行了相似度计算和聚类分析,结果表明,所建立的秦艽指纹图谱可用于秦艽药材的真伪鉴别和质量评价.