Our relationship with the colonic bacterial flora has long been viewed as benign, but recent studies suggest that this symbiosis has risks as well as benefits. This relationship requires that the host not only provide...Our relationship with the colonic bacterial flora has long been viewed as benign, but recent studies suggest that this symbiosis has risks as well as benefits. This relationship requires that the host not only provide a supportive environment for the symbiotic bacteria, but also actively maintain intact mechanisms for properly managing the physiologic stresses that are closely associated with the symbiont’s essential survival functions. Failure to do so breaches the host- symbiont contract, and can result in serious effects on the health of the host. Recent investigations that employ several knockout mouse models reveal the consequences of genetic deficiency in the host regarding these mechanisms, and the latent, pro-inflammatory, tumorigenic nature of normal bacterial flora. Further study of the interactions between normal bacterial flora and hosts could shed light on the etiologies and pathogenesis of inflammatory diseases and related cancers, with implications for human health.展开更多
The technique of embryo transfer has been evaluated for the purpose of changing the mouse stocks to a germfree(GF)status.Our results showreproducible and quality-assured conversion of animalstothose which are negative...The technique of embryo transfer has been evaluated for the purpose of changing the mouse stocks to a germfree(GF)status.Our results showreproducible and quality-assured conversion of animalstothose which are negativeforthe presence of microorganisms.Rapid and easy access to GF mice is advantageous for studies of selected microflora andtheir cross-talks withthe host,when applying,e.g.genomic,proteomic and metabolic methodology.The studyinvolved embryotransfer in anisolator environment,thereby allowingimplantationof cleansed embryosinto GFrecipients under well-controlled conditions.The recipientfemales gave birth normally andtookcare of the offspringasif they were their own pups,thus enhancing the survival rate.Access to full technical resources required to maintain GFisolators are,however,a prerequisite.In this study,we used stainless steel isolators designed by Gustafsson(1959),on which a stereomicroscope wasmountedtofacilitate embryo transfer inside the isolator.The use of embryo transfer and isolator techniques will facilitate the availability ofvarious mouse mutant models under different gnotobiotic conditions,GF,monoxenic or polyxenic animals,to enable comparison withconventional animals for physiological and pathophysiological studies.展开更多
基金Supported by US Public Health Service Grants, R01CA97946 and R01AI063477 and the Medical Research Service of the Department of Veterans Affairs, United States
文摘Our relationship with the colonic bacterial flora has long been viewed as benign, but recent studies suggest that this symbiosis has risks as well as benefits. This relationship requires that the host not only provide a supportive environment for the symbiotic bacteria, but also actively maintain intact mechanisms for properly managing the physiologic stresses that are closely associated with the symbiont’s essential survival functions. Failure to do so breaches the host- symbiont contract, and can result in serious effects on the health of the host. Recent investigations that employ several knockout mouse models reveal the consequences of genetic deficiency in the host regarding these mechanisms, and the latent, pro-inflammatory, tumorigenic nature of normal bacterial flora. Further study of the interactions between normal bacterial flora and hosts could shed light on the etiologies and pathogenesis of inflammatory diseases and related cancers, with implications for human health.
文摘The technique of embryo transfer has been evaluated for the purpose of changing the mouse stocks to a germfree(GF)status.Our results showreproducible and quality-assured conversion of animalstothose which are negativeforthe presence of microorganisms.Rapid and easy access to GF mice is advantageous for studies of selected microflora andtheir cross-talks withthe host,when applying,e.g.genomic,proteomic and metabolic methodology.The studyinvolved embryotransfer in anisolator environment,thereby allowingimplantationof cleansed embryosinto GFrecipients under well-controlled conditions.The recipientfemales gave birth normally andtookcare of the offspringasif they were their own pups,thus enhancing the survival rate.Access to full technical resources required to maintain GFisolators are,however,a prerequisite.In this study,we used stainless steel isolators designed by Gustafsson(1959),on which a stereomicroscope wasmountedtofacilitate embryo transfer inside the isolator.The use of embryo transfer and isolator techniques will facilitate the availability ofvarious mouse mutant models under different gnotobiotic conditions,GF,monoxenic or polyxenic animals,to enable comparison withconventional animals for physiological and pathophysiological studies.
