BACKGROUND Giant cell tumor of bone is a locally aggressive and rarely metastasizing tumor,and also a potential malignant tumor that may develop into a primary malignant giant cell tumor.AIM To evaluate the role of mu...BACKGROUND Giant cell tumor of bone is a locally aggressive and rarely metastasizing tumor,and also a potential malignant tumor that may develop into a primary malignant giant cell tumor.AIM To evaluate the role of multimodal imaging in the diagnosis of giant cell tumors of bone.METHODS The data of 32 patients with giant cell tumor of bone confirmed by core-needle biopsy or surgical pathology at our hospital between March 2018 and March 2023 were retrospectively selected.All the patients with giant cell tumors of the bone were examined by X-ray,computed tomography(CT)and magnetic resonance imaging(MRI),and 7 of them were examined by positron emission tomography(PET)-CT.RESULTS X-ray imaging can provide overall information on giant cell tumor lesions.CT and MRI can reveal the characteristics of the internal structure of the tumor as well as the adjacent relationships of the tumor,and these methods have unique advantages for diagnosing tumors and determining the scope of surgery.PET-CT can detect small lesions and is highly valuable for identifying benign and malignant tumors to aid in the early diagnosis of metastasis.CONCLUSION Multimodal imaging plays an important role in the diagnosis of giant cell tumor of bone and can provide a reference for the treatment of giant cell tumors.展开更多
Primary malignant giant cell tumor of bone is clinically rare,lack of specificity,and often misdiagnosed.Currently,related literature about this tumor remains scarce.One case of primary malignant giant cell tumor of b...Primary malignant giant cell tumor of bone is clinically rare,lack of specificity,and often misdiagnosed.Currently,related literature about this tumor remains scarce.One case of primary malignant giant cell tumor of bone was diagnosed and treated in our hospital,and the treatment effect was satisfactory.There was no recurrence or metastasis in 2 years of followup.The report is as follows.展开更多
BACKGROUND Giant cell tumor(GCT)is a benign lesion and rarely involves the patella.This disease is characterized by a relatively high recurrence rate after primary treatment.En bloc resection has been a predominant op...BACKGROUND Giant cell tumor(GCT)is a benign lesion and rarely involves the patella.This disease is characterized by a relatively high recurrence rate after primary treatment.En bloc resection has been a predominant option for recurrent GCT.However,total patellectomy can lead to disruption of the knee.Therefore,exploration of functional reconstruction of the extensor mechanism is worthwhile.CASE SUMMARY A 54-year-old woman presented with right knee pain and swelling,and was diagnosed as having a GCT in the patella following curettage and autograft.Medical imaging revealed a lytic and expanded lesion involving the whole patella with focal cortical breaches and pathological fracture.Based on the combination of histological,radiological,and clinical features,a diagnosis of recurrent GCT in the patella was made(Campanacci grade III).After a multidisciplinary team discussion,three-dimensional(3D)-printed custom-made patellar endoprosthesis was performed following en bloc resection for reconstructing the extensor mechanism.The patient was followed for 35 mo postoperatively.No evidence of local recurrence,pulmonary metastasis,or osteoarthritis of the right knee was observed.The active flexion arc was 0°-120°,and no extension lag was detected.A favorable patellar tracking and height(Insall-Salvati ratio 0.93)were detected by radiography.CONCLUSION We depict a case of a GCT at the right patella,which was successfully treated by patellectomy and 3D-printed custom-made endoprosthetic replacement.The patella normal reconstruction,the precise-fit articular design,and gastrocnemius flap augmentation could lead to satisfactory knee function and a low rate of complications in the short-term follow-up.展开更多
Giant cell tumors of bone(GCTB)are associated with massive bone destructions and high recurrence rates.In a previous study,we observed cytotoxic effects of three different compositions of bioactive glasses(BGs)towards...Giant cell tumors of bone(GCTB)are associated with massive bone destructions and high recurrence rates.In a previous study,we observed cytotoxic effects of three different compositions of bioactive glasses(BGs)towards GCTSC but not bone marrow derived stromal cells(BMSC)indicating that BGs represent promising candidates for the development of new therapeutic approaches.In the current study we aimed to investigate the molecular mechanisms that are involved in BG induced cytotoxicity.We observed,that BG treatment was not associated with any signs of apoptosis,but rather led to a strong induction of mitogen activated protein kinases(MAPK)and,as a consequence,upregulation of several transcription factors specifically in GCTSC.Genome wide gene expression profiling further revealed a set of fifteen genes that were exclusively induced in GCTSC or induced significantly stronger in GCTSC compared to BMSC.BG treatment further induced autophagy that was significantly more pronounced in GCTSC compared to BMSC and could be inhibited by MAPK inhibitors.Together with the known osteogenic properties of BGs our findings support the suitability of BGs as therapeutic agents for the treatment of GCTB.However,these data have to be verified under in vivo conditions.展开更多
The aim of this paper was to study the differential gene expression of giant cell tumor of bone(GCTB)by gene chip technology.Total RNA of 8 fresh GCTB specimens(Jaffe I∶6 cases,II∶1 case,III∶1 case;Campanacci I∶6 ...The aim of this paper was to study the differential gene expression of giant cell tumor of bone(GCTB)by gene chip technology.Total RNA of 8 fresh GCTB specimens(Jaffe I∶6 cases,II∶1 case,III∶1 case;Campanacci I∶6 cases,II∶1 case,III∶1 case;Enneking Staging G0T1-2M0,5 cases,G1T1-2M0:2 cases,G1T2M0:1 case)and 4 normal bony callus specimens(the control group)were extracted and purified to get mRNA and then reverse transcribed to complementary DNA,respectively.Microarray screening with a set of 8064 human cDNA genes was conducted to analyze the difference among the samples and the control.The hybridization signals were scanned.The gene expression disparity between the GCTB samples and normal bony callus was significantly different(P<0.01),and the disparity of over 5-fold was found in 47 genes in the GCTB specimens,with 25 genes up-regulated and 22 down-regulated including the extracellular matrix and transforming-related genes,oncogene and its homolog genes,cytokine and its receptor genes.Specific gene spectrum associated with GCTB can be identified by cDNA microarray,which will be the foundation of progressive etiology elucidation,diagnosis and treatment of GCTB.展开更多
基金Supported by the Technology Innovation Leading Program of Shaanxi,No.2023KXJ-095the Shaanxi Provincial People's Hospital Science and Technology Talent Support Program for Elite Talents,No.2021JY-38 and No.2021JY-50the Shaanxi Provincial People's Hospital Science and Technology Development Incubation Foundation,No.2023YJY-39.
文摘BACKGROUND Giant cell tumor of bone is a locally aggressive and rarely metastasizing tumor,and also a potential malignant tumor that may develop into a primary malignant giant cell tumor.AIM To evaluate the role of multimodal imaging in the diagnosis of giant cell tumors of bone.METHODS The data of 32 patients with giant cell tumor of bone confirmed by core-needle biopsy or surgical pathology at our hospital between March 2018 and March 2023 were retrospectively selected.All the patients with giant cell tumors of the bone were examined by X-ray,computed tomography(CT)and magnetic resonance imaging(MRI),and 7 of them were examined by positron emission tomography(PET)-CT.RESULTS X-ray imaging can provide overall information on giant cell tumor lesions.CT and MRI can reveal the characteristics of the internal structure of the tumor as well as the adjacent relationships of the tumor,and these methods have unique advantages for diagnosing tumors and determining the scope of surgery.PET-CT can detect small lesions and is highly valuable for identifying benign and malignant tumors to aid in the early diagnosis of metastasis.CONCLUSION Multimodal imaging plays an important role in the diagnosis of giant cell tumor of bone and can provide a reference for the treatment of giant cell tumors.
基金on the treatment ofknee osteoarthritis by Kangjian5s three-dimensional model(2020XZ003)。
文摘Primary malignant giant cell tumor of bone is clinically rare,lack of specificity,and often misdiagnosed.Currently,related literature about this tumor remains scarce.One case of primary malignant giant cell tumor of bone was diagnosed and treated in our hospital,and the treatment effect was satisfactory.There was no recurrence or metastasis in 2 years of followup.The report is as follows.
基金National Key Research and Development Program of China,No.2016YFC1102003Science and Technology Research Program of Sichuan Province,No.2020YFS0036+2 种基金Chengdu Science and Technology Program Projects,No.2017-CY02-00032-GXNational Natural Science Foundation of China,No.81801852National Key Research and Development Program of China,No.2017YFB0702604.
文摘BACKGROUND Giant cell tumor(GCT)is a benign lesion and rarely involves the patella.This disease is characterized by a relatively high recurrence rate after primary treatment.En bloc resection has been a predominant option for recurrent GCT.However,total patellectomy can lead to disruption of the knee.Therefore,exploration of functional reconstruction of the extensor mechanism is worthwhile.CASE SUMMARY A 54-year-old woman presented with right knee pain and swelling,and was diagnosed as having a GCT in the patella following curettage and autograft.Medical imaging revealed a lytic and expanded lesion involving the whole patella with focal cortical breaches and pathological fracture.Based on the combination of histological,radiological,and clinical features,a diagnosis of recurrent GCT in the patella was made(Campanacci grade III).After a multidisciplinary team discussion,three-dimensional(3D)-printed custom-made patellar endoprosthesis was performed following en bloc resection for reconstructing the extensor mechanism.The patient was followed for 35 mo postoperatively.No evidence of local recurrence,pulmonary metastasis,or osteoarthritis of the right knee was observed.The active flexion arc was 0°-120°,and no extension lag was detected.A favorable patellar tracking and height(Insall-Salvati ratio 0.93)were detected by radiography.CONCLUSION We depict a case of a GCT at the right patella,which was successfully treated by patellectomy and 3D-printed custom-made endoprosthetic replacement.The patella normal reconstruction,the precise-fit articular design,and gastrocnemius flap augmentation could lead to satisfactory knee function and a low rate of complications in the short-term follow-up.
基金funded by a grant from German Cancer Aid(Deutsche Krebshilfe,grant number 70113788)。
文摘Giant cell tumors of bone(GCTB)are associated with massive bone destructions and high recurrence rates.In a previous study,we observed cytotoxic effects of three different compositions of bioactive glasses(BGs)towards GCTSC but not bone marrow derived stromal cells(BMSC)indicating that BGs represent promising candidates for the development of new therapeutic approaches.In the current study we aimed to investigate the molecular mechanisms that are involved in BG induced cytotoxicity.We observed,that BG treatment was not associated with any signs of apoptosis,but rather led to a strong induction of mitogen activated protein kinases(MAPK)and,as a consequence,upregulation of several transcription factors specifically in GCTSC.Genome wide gene expression profiling further revealed a set of fifteen genes that were exclusively induced in GCTSC or induced significantly stronger in GCTSC compared to BMSC.BG treatment further induced autophagy that was significantly more pronounced in GCTSC compared to BMSC and could be inhibited by MAPK inhibitors.Together with the known osteogenic properties of BGs our findings support the suitability of BGs as therapeutic agents for the treatment of GCTB.However,these data have to be verified under in vivo conditions.
基金supported by a grant from the Shenzhen Municipal Research Program of Sciences and Technology(No.200204061).
文摘The aim of this paper was to study the differential gene expression of giant cell tumor of bone(GCTB)by gene chip technology.Total RNA of 8 fresh GCTB specimens(Jaffe I∶6 cases,II∶1 case,III∶1 case;Campanacci I∶6 cases,II∶1 case,III∶1 case;Enneking Staging G0T1-2M0,5 cases,G1T1-2M0:2 cases,G1T2M0:1 case)and 4 normal bony callus specimens(the control group)were extracted and purified to get mRNA and then reverse transcribed to complementary DNA,respectively.Microarray screening with a set of 8064 human cDNA genes was conducted to analyze the difference among the samples and the control.The hybridization signals were scanned.The gene expression disparity between the GCTB samples and normal bony callus was significantly different(P<0.01),and the disparity of over 5-fold was found in 47 genes in the GCTB specimens,with 25 genes up-regulated and 22 down-regulated including the extracellular matrix and transforming-related genes,oncogene and its homolog genes,cytokine and its receptor genes.Specific gene spectrum associated with GCTB can be identified by cDNA microarray,which will be the foundation of progressive etiology elucidation,diagnosis and treatment of GCTB.