With the emergence and rapid development of nanotechnology,the nanoparticles hybridized with multicomponent lipids are more and more used in gene delivery.These vectors interact with the cell membrane before entering ...With the emergence and rapid development of nanotechnology,the nanoparticles hybridized with multicomponent lipids are more and more used in gene delivery.These vectors interact with the cell membrane before entering into the cell.Therefore,the nature of this interaction is important in investigating multicomponent liposome-nanoparticle(MLP)transport across the cell membrane.In this paper the transport of MLPs across the membranes of giant vesicles(GVs)in solvents is studied by using the self-consistent field theory(SCFT).Based on the analysis of the MLP permeating the GV membranes,a simple transport model is proposed.The effects of the difference in membrane morphology and the size of the nanoparticle on the endocytosis are discussed systematically.The role of energy barriers in quasi-equilibrium is also examined.The results indicate that the interaction between MLP and GV is a spontaneous process and the energy barrier needs overcoming to form metastable intermediates.The results provide theoretical reference for better understanding the transmembrane transport process of nanoparticles,and guidance for relevant experimental studies as well.展开更多
基金Project supported by the Science and Technology Innovation Project of Higher Education in Shanxi Province,China(Grant No.2020L0752)the Basic Research Foundation Project in Modern College of Humanities and Sciences of Shanxi Normal University,China(Grant No.2020JCYJ25)the Graduate Student Science and Technology Innovation Project of Shanxi Normal University,China(Grant No.01053011)。
文摘With the emergence and rapid development of nanotechnology,the nanoparticles hybridized with multicomponent lipids are more and more used in gene delivery.These vectors interact with the cell membrane before entering into the cell.Therefore,the nature of this interaction is important in investigating multicomponent liposome-nanoparticle(MLP)transport across the cell membrane.In this paper the transport of MLPs across the membranes of giant vesicles(GVs)in solvents is studied by using the self-consistent field theory(SCFT).Based on the analysis of the MLP permeating the GV membranes,a simple transport model is proposed.The effects of the difference in membrane morphology and the size of the nanoparticle on the endocytosis are discussed systematically.The role of energy barriers in quasi-equilibrium is also examined.The results indicate that the interaction between MLP and GV is a spontaneous process and the energy barrier needs overcoming to form metastable intermediates.The results provide theoretical reference for better understanding the transmembrane transport process of nanoparticles,and guidance for relevant experimental studies as well.
