Tomato is an ideal model species for fleshy fruit development research. Sl YABBY2 b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between Sl YABBY2 b and e...Tomato is an ideal model species for fleshy fruit development research. Sl YABBY2 b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between Sl YABBY2 b and endogenous gibberellin is poorly understood. In this study, Sl YABBY2 b-overexpressing and RNA interference(RNAi) transgenic tomato plants were used to elucidate the mechanism by which Sl YABBY2 b regulates the ovary locule number and endogenous gibberellin content in tomato. Sl YABBY2 b-overexpressing plants showed fewer locules and lower gibberellin content than the control plants. Contrasting results were found in the RNAi lines. Therefore, the Sl YABBY2 b gene negatively regulates tomato ovary locule number and endogenous gibberellin content. Furthermore, the expression of Sl YABBY2 b gene was remarkably higher than that of the wild type in the apical shoots of gibberellindeficient mutants. This showed that the gibberellins can inhibit the expression of Sl YABBY2 b gene negative regulation. Further study revealed that Sl YABBY2 b suppressed the expression of Sl GA20 ox1 and Sl GA3 ox2, but increased that of Sl GA2 ox1 and Sl GA2 ox5 in the apical shoots of Sl YABBY2 b-overexpressing plants, thereby reducing gibberellin content. Contrasting results were found in the RNAi lines. Our results showed that the Sl YABBY2 b gene was located on gibberellin signal transduction pathways, fed back regulation of the synthesis of gibberellin, and felt exogenous gibberellin signal to further regulate the formation of tomato locule.展开更多
基金Project supported by the China Agriculture Research System(No.CARS-25)the Program for Liaoning Innovative Research Team in University(No.LZ2015025)the Program for Liaoning Key Laboratory(No.LZ2015064),China
文摘Tomato is an ideal model species for fleshy fruit development research. Sl YABBY2 b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between Sl YABBY2 b and endogenous gibberellin is poorly understood. In this study, Sl YABBY2 b-overexpressing and RNA interference(RNAi) transgenic tomato plants were used to elucidate the mechanism by which Sl YABBY2 b regulates the ovary locule number and endogenous gibberellin content in tomato. Sl YABBY2 b-overexpressing plants showed fewer locules and lower gibberellin content than the control plants. Contrasting results were found in the RNAi lines. Therefore, the Sl YABBY2 b gene negatively regulates tomato ovary locule number and endogenous gibberellin content. Furthermore, the expression of Sl YABBY2 b gene was remarkably higher than that of the wild type in the apical shoots of gibberellindeficient mutants. This showed that the gibberellins can inhibit the expression of Sl YABBY2 b gene negative regulation. Further study revealed that Sl YABBY2 b suppressed the expression of Sl GA20 ox1 and Sl GA3 ox2, but increased that of Sl GA2 ox1 and Sl GA2 ox5 in the apical shoots of Sl YABBY2 b-overexpressing plants, thereby reducing gibberellin content. Contrasting results were found in the RNAi lines. Our results showed that the Sl YABBY2 b gene was located on gibberellin signal transduction pathways, fed back regulation of the synthesis of gibberellin, and felt exogenous gibberellin signal to further regulate the formation of tomato locule.
