By optimizing the separation and analytical conditions, a reliable, simple and accurate high-performance liquid chromatography（HPLC） method coupled with ultraviolet（UV） and evaporative light scattering detector（E...By optimizing the separation and analytical conditions, a reliable, simple and accurate high-performance liquid chromatography（HPLC） method coupled with ultraviolet（UV） and evaporative light scattering detector（ELSD） was developed for the simultaneous determination of lactones and flavonoid, that is, bilobalide, ginkgolide A, ginkgolide B, and rutin, in more than 10 batches of Ginkgo biloba tablets from different pharmaceutical companies. The method could also be applied to fingerprint research, for a more general evaluation of the quality of this preparation. The separation of the components was achieved on a Hanbon C18 column with gradient elution using water and methanol containing 0.1% trifluoroacetic acid（TFA）. The column temperature was 30 ℃ and the flow-rate of the mobile phase was 0.6 mL/min. The drift tube temperature of the ELSD was set at 100 ℃, and the nitrogen flow-rate was 2 L/min. Good linear relationships were shown with correlation coefficients for analytes exceeding 0.9913. The limits of detection（LODs, S/N=3） and the limits of quantitation（LOQs, S/N=10） were 0.00887-0.0508 μg/μL and 0.0171- 0.0636 μg/μL, respectively, on the column. The relative standard deviations（RSD） of the analytes were less than 3.61% for intraday and 3.70% for interday, respectively. The average recovery rates obtained were in the range of （97.3±4.3）% to （101.9±3.1）% for all the compounds. The results of quantitative and fingerprint analysis proved that the contents of the components were totally similar in the preparation of Ginkgo biloba tablets from the same pharmaceutical company; whereas, they varied significantly in the preparations of Ginkgo biloba tablets from different companies.展开更多
The relationship between the adsorption properties for the active components of ginkgo leaves and the structure of the adsorbents based on urea-formaldehyde condensed polymers was investigated .The results revealed th...The relationship between the adsorption properties for the active components of ginkgo leaves and the structure of the adsorbents based on urea-formaldehyde condensed polymers was investigated .The results revealed that these adsorbents showed very hjgh adsorption selectivity for both flavonol glycosides and terpene lactones contained in ginkgo leaves. Thus, an adsorption separation procedure for purification of ginkgo leaves extracts was developed.展开更多
Terpene lactone contents in different organs of Ginkgo biloba L. and leaf, stem and root of G. biloba seedlings treated with chlorocholine chloride were determined by GC. The results showed that total terpene lactone ...Terpene lactone contents in different organs of Ginkgo biloba L. and leaf, stem and root of G. biloba seedlings treated with chlorocholine chloride were determined by GC. The results showed that total terpene lactone contents in the order from high to low were bud (1.973 mg·g-1), leaf (1.753 mg·g-1), root, stem, and the terpene lactone content in phloem was higher than that in xylem. Leaf contained more bilobalide than root and stem. Chlorocholine chloride treatment significantly increased terpene lactone content of G. biloba seedlings. The possible way of terpene lactone biosynthesis was discussed.展开更多
基金Supported by the Program for New Century Excellent Talents in University of China(No.NCET-06-0515).
文摘By optimizing the separation and analytical conditions, a reliable, simple and accurate high-performance liquid chromatography（HPLC） method coupled with ultraviolet（UV） and evaporative light scattering detector（ELSD） was developed for the simultaneous determination of lactones and flavonoid, that is, bilobalide, ginkgolide A, ginkgolide B, and rutin, in more than 10 batches of Ginkgo biloba tablets from different pharmaceutical companies. The method could also be applied to fingerprint research, for a more general evaluation of the quality of this preparation. The separation of the components was achieved on a Hanbon C18 column with gradient elution using water and methanol containing 0.1% trifluoroacetic acid（TFA）. The column temperature was 30 ℃ and the flow-rate of the mobile phase was 0.6 mL/min. The drift tube temperature of the ELSD was set at 100 ℃, and the nitrogen flow-rate was 2 L/min. Good linear relationships were shown with correlation coefficients for analytes exceeding 0.9913. The limits of detection（LODs, S/N=3） and the limits of quantitation（LOQs, S/N=10） were 0.00887-0.0508 μg/μL and 0.0171- 0.0636 μg/μL, respectively, on the column. The relative standard deviations（RSD） of the analytes were less than 3.61% for intraday and 3.70% for interday, respectively. The average recovery rates obtained were in the range of （97.3±4.3）% to （101.9±3.1）% for all the compounds. The results of quantitative and fingerprint analysis proved that the contents of the components were totally similar in the preparation of Ginkgo biloba tablets from the same pharmaceutical company; whereas, they varied significantly in the preparations of Ginkgo biloba tablets from different companies.
基金Supported by the National Natural Science Foundation of China !(Grant No. 29574164).
文摘The relationship between the adsorption properties for the active components of ginkgo leaves and the structure of the adsorbents based on urea-formaldehyde condensed polymers was investigated .The results revealed that these adsorbents showed very hjgh adsorption selectivity for both flavonol glycosides and terpene lactones contained in ginkgo leaves. Thus, an adsorption separation procedure for purification of ginkgo leaves extracts was developed.
文摘Terpene lactone contents in different organs of Ginkgo biloba L. and leaf, stem and root of G. biloba seedlings treated with chlorocholine chloride were determined by GC. The results showed that total terpene lactone contents in the order from high to low were bud (1.973 mg·g-1), leaf (1.753 mg·g-1), root, stem, and the terpene lactone content in phloem was higher than that in xylem. Leaf contained more bilobalide than root and stem. Chlorocholine chloride treatment significantly increased terpene lactone content of G. biloba seedlings. The possible way of terpene lactone biosynthesis was discussed.
