Ginkgo biloba seeds were widely used for food and traditional medicine in china. In the present study, antioxidant proteins named GP were purified from Ginkgo biloba seeds. The proteins （GP） were purified by homogen...Ginkgo biloba seeds were widely used for food and traditional medicine in china. In the present study, antioxidant proteins named GP were purified from Ginkgo biloba seeds. The proteins （GP） were purified by homogenization of Ginkgo biloba seeds powder in the saline solution, precipitation with 70% ammonium sulphate, and filtration on DEAE-Cellulose 52 anion exchange column. GP showed protein content of 96%, and they showed three bands with approximate molecular weight of 15 kDa, 17 kDa, 21 kDa respectively by SDS-PAGE electrophoresis analysis. Compositions of amino acids obtained by Amino Acid Analyzer showed GP were rich in Glu, Ser ,His ,Asp, Gly, Ala, Leu and Lys compared with the other fractions. The antioxidant activities of GP were investigated using DPPH （the 1, 1-diphenyl-2-picrylhydrazyl） free radical-scavenging and ferric-reducing antioxidant power assays. The GP showed antioxidant activities higher than α-Tocopherol and BHA. Therefore, the present study demonstrates proteins from Ginkgo biloba seeds （GP） can be excellent antioxidant.展开更多
Germination tests are currently the most widely used method to evaluate seed quality of Ginkgo biloba L., but they are time-consuming and labor intensive. Oxygen-sensing technology, based on the principle of fluoresce...Germination tests are currently the most widely used method to evaluate seed quality of Ginkgo biloba L., but they are time-consuming and labor intensive. Oxygen-sensing technology, based on the principle of fluorescence quenching to detect oxygen and assess seed quality was used to rapidly evaluate seed quality of two varieties (Shandong Tancheng 202 and Zhejiang Dafoshou) of G. biloba from five mother plants. Fifteen samples of three vigor levels were produced by accelerated aging treatments. This process was applied in duplicate. A portable oxygen-sensing detector was employed to measure oxygen content during seed germination in a closed system at 25 A degrees C each day until day 14. Four oxygen metabolism indices were calculated: oxygen consumption index, oxygen consumption rate, critical oxygen concentration, and theoretical germination time (T (GT)). Additionally, we tested laboratory germination and field emergence. The results of a one-way analysis of variance and correlation analysis showed that T (GT) was the candidate index to evaluate seed quality of G. biloba. Therefore, the T (GT) value was used to validate the reliability of oxygen-sensing evaluation for Zhejiang Dafoshou seeds kept under four storage conditions. The trend in the change in oxygen metabolism agreed completely agreement with that of seed germination under all storage conditions. The oxygen-sensing test reliably and rapidly assessed seed quality of G. biloba. The germination rate of Zhejiang Dafoshou was accurately predicted by T (GT).展开更多
The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis （2-DE）. The results showed that 80 kinds of p...The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis （2-DE）. The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pIr.8, 31kD/pIr.8, 29kD/pIr.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins （VSPs）.展开更多
Ginkgo biloba is a dioecious and unique species within Ginkgo family and Ginkgo genus, but it shows a big variability. From 1950s, resources of female plants in China were investigated and collected, and the seed ana...Ginkgo biloba is a dioecious and unique species within Ginkgo family and Ginkgo genus, but it shows a big variability. From 1950s, resources of female plants in China were investigated and collected, and the seed anatomical structure and morphological characters at different development stages were observed. According to the shape, length, width and thickness of ovule bracket and seed stone, as well as the characteristics of both sides and top of the stone, the female plants of Ginkgo biloba have been divided into five types, long-stone, Buddha-finger, horse-bell, mume-stone and round-stone. In addition, the important cultivars belonging to different types were also determined. The stone weight, index of stone shape, and contents of dry matter, amylose, amylopectin, starch, rough protein, soluble sugar and hydrocyanic acid in seed kernel of each type were measured, and the qualities of seed stone were appraised, which lays a foundation for the improvement and utilization of female plant resources in Ginkgo biloba.展开更多
文摘Ginkgo biloba seeds were widely used for food and traditional medicine in china. In the present study, antioxidant proteins named GP were purified from Ginkgo biloba seeds. The proteins （GP） were purified by homogenization of Ginkgo biloba seeds powder in the saline solution, precipitation with 70% ammonium sulphate, and filtration on DEAE-Cellulose 52 anion exchange column. GP showed protein content of 96%, and they showed three bands with approximate molecular weight of 15 kDa, 17 kDa, 21 kDa respectively by SDS-PAGE electrophoresis analysis. Compositions of amino acids obtained by Amino Acid Analyzer showed GP were rich in Glu, Ser ,His ,Asp, Gly, Ala, Leu and Lys compared with the other fractions. The antioxidant activities of GP were investigated using DPPH （the 1, 1-diphenyl-2-picrylhydrazyl） free radical-scavenging and ferric-reducing antioxidant power assays. The GP showed antioxidant activities higher than α-Tocopherol and BHA. Therefore, the present study demonstrates proteins from Ginkgo biloba seeds （GP） can be excellent antioxidant.
基金supported by the National Natural Science Foundation of China(31371712,30800890)Natural Science Foundation of Zhejiang Province(LY13C130011,LY13C16007)+2 种基金Special Fund for Agro-scientific Research in the Public Interest of China(201303002)the Graduate Special Fund Innovative Projects of Jiangxi Province(YC2014-B035)the University Teachers’Professional Development Project of Zhejiang Province(FX2015050)
文摘Germination tests are currently the most widely used method to evaluate seed quality of Ginkgo biloba L., but they are time-consuming and labor intensive. Oxygen-sensing technology, based on the principle of fluorescence quenching to detect oxygen and assess seed quality was used to rapidly evaluate seed quality of two varieties (Shandong Tancheng 202 and Zhejiang Dafoshou) of G. biloba from five mother plants. Fifteen samples of three vigor levels were produced by accelerated aging treatments. This process was applied in duplicate. A portable oxygen-sensing detector was employed to measure oxygen content during seed germination in a closed system at 25 A degrees C each day until day 14. Four oxygen metabolism indices were calculated: oxygen consumption index, oxygen consumption rate, critical oxygen concentration, and theoretical germination time (T (GT)). Additionally, we tested laboratory germination and field emergence. The results of a one-way analysis of variance and correlation analysis showed that T (GT) was the candidate index to evaluate seed quality of G. biloba. Therefore, the T (GT) value was used to validate the reliability of oxygen-sensing evaluation for Zhejiang Dafoshou seeds kept under four storage conditions. The trend in the change in oxygen metabolism agreed completely agreement with that of seed germination under all storage conditions. The oxygen-sensing test reliably and rapidly assessed seed quality of G. biloba. The germination rate of Zhejiang Dafoshou was accurately predicted by T (GT).
基金The research was supported by National Natural Science Foundation of China (30371154).
文摘The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis （2-DE）. The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pIr.8, 31kD/pIr.8, 29kD/pIr.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins （VSPs）.
文摘Ginkgo biloba is a dioecious and unique species within Ginkgo family and Ginkgo genus, but it shows a big variability. From 1950s, resources of female plants in China were investigated and collected, and the seed anatomical structure and morphological characters at different development stages were observed. According to the shape, length, width and thickness of ovule bracket and seed stone, as well as the characteristics of both sides and top of the stone, the female plants of Ginkgo biloba have been divided into five types, long-stone, Buddha-finger, horse-bell, mume-stone and round-stone. In addition, the important cultivars belonging to different types were also determined. The stone weight, index of stone shape, and contents of dry matter, amylose, amylopectin, starch, rough protein, soluble sugar and hydrocyanic acid in seed kernel of each type were measured, and the qualities of seed stone were appraised, which lays a foundation for the improvement and utilization of female plant resources in Ginkgo biloba.
