Germination tests are currently the most widely used method to evaluate seed quality of Ginkgo biloba L., but they are time-consuming and labor intensive. Oxygen-sensing technology, based on the principle of fluoresce...Germination tests are currently the most widely used method to evaluate seed quality of Ginkgo biloba L., but they are time-consuming and labor intensive. Oxygen-sensing technology, based on the principle of fluorescence quenching to detect oxygen and assess seed quality was used to rapidly evaluate seed quality of two varieties (Shandong Tancheng 202 and Zhejiang Dafoshou) of G. biloba from five mother plants. Fifteen samples of three vigor levels were produced by accelerated aging treatments. This process was applied in duplicate. A portable oxygen-sensing detector was employed to measure oxygen content during seed germination in a closed system at 25 A degrees C each day until day 14. Four oxygen metabolism indices were calculated: oxygen consumption index, oxygen consumption rate, critical oxygen concentration, and theoretical germination time (T (GT)). Additionally, we tested laboratory germination and field emergence. The results of a one-way analysis of variance and correlation analysis showed that T (GT) was the candidate index to evaluate seed quality of G. biloba. Therefore, the T (GT) value was used to validate the reliability of oxygen-sensing evaluation for Zhejiang Dafoshou seeds kept under four storage conditions. The trend in the change in oxygen metabolism agreed completely agreement with that of seed germination under all storage conditions. The oxygen-sensing test reliably and rapidly assessed seed quality of G. biloba. The germination rate of Zhejiang Dafoshou was accurately predicted by T (GT).展开更多
The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis （2-DE）. The results showed that 80 kinds of p...The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis （2-DE）. The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pIr.8, 31kD/pIr.8, 29kD/pIr.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins （VSPs）.展开更多
In order to explore the best storage temperature and storage period of dragon fruit seeds,with the constant-humidity germplasm cold storage of Guangxi Academy of Agricultural Sciences as a platform and storage tempera...In order to explore the best storage temperature and storage period of dragon fruit seeds,with the constant-humidity germplasm cold storage of Guangxi Academy of Agricultural Sciences as a platform and storage temperature as an environmental factor,the germination characteristics of dragon fruit seeds and seedling growth characteristics under low temperature storage(8,4,-1,-10℃)and room temperature storage(25-30℃)over time were explored.The results showed that with the extension of the storage time,the seed germination rates,germination index,healthy seedling rates,seedling heights and fresh weights of 10 seedlings of the three dragon fruit varieties showed basically the same trends,showing a downward trend.After 367 d of cold storage,the germination rate decreased from 100%to 92.33%-98.00%,and the room temperature treatments decreased to 54.00%-86.30%;and the healthy seedling rates of cold-stored seeds decreased from 96.33%-98.00%to 77.33%-97.3%,and the values of the room temperature treatments dropped to 12.00%-68.33%.The germination rates,germination index,healthy seedling rates and seedling heights of the three dragon fruit varieties stored at low temperatures were basically significantly higher than those of the room temperature treatments within 367 d of storage.The germination rates of the three varieties stored at 8℃had no significant differences from other low temperature treatments,but the germination index was significantly lower than other low temperature treatments at 367 d.It showed that low temperature storage at-10-8℃could significantly prolong the vigor and storage time of dragon fruit seeds.Considering cost and other factors,it is better to store dragon fruit seeds in a dry environment at 4℃.The seeds stored at 4℃for 1 year showed a germination rate maintained over 90%and a healthy seedling rate over 85%.展开更多
基金supported by the National Natural Science Foundation of China(31371712,30800890)Natural Science Foundation of Zhejiang Province(LY13C130011,LY13C16007)+2 种基金Special Fund for Agro-scientific Research in the Public Interest of China(201303002)the Graduate Special Fund Innovative Projects of Jiangxi Province(YC2014-B035)the University Teachers’Professional Development Project of Zhejiang Province(FX2015050)
文摘Germination tests are currently the most widely used method to evaluate seed quality of Ginkgo biloba L., but they are time-consuming and labor intensive. Oxygen-sensing technology, based on the principle of fluorescence quenching to detect oxygen and assess seed quality was used to rapidly evaluate seed quality of two varieties (Shandong Tancheng 202 and Zhejiang Dafoshou) of G. biloba from five mother plants. Fifteen samples of three vigor levels were produced by accelerated aging treatments. This process was applied in duplicate. A portable oxygen-sensing detector was employed to measure oxygen content during seed germination in a closed system at 25 A degrees C each day until day 14. Four oxygen metabolism indices were calculated: oxygen consumption index, oxygen consumption rate, critical oxygen concentration, and theoretical germination time (T (GT)). Additionally, we tested laboratory germination and field emergence. The results of a one-way analysis of variance and correlation analysis showed that T (GT) was the candidate index to evaluate seed quality of G. biloba. Therefore, the T (GT) value was used to validate the reliability of oxygen-sensing evaluation for Zhejiang Dafoshou seeds kept under four storage conditions. The trend in the change in oxygen metabolism agreed completely agreement with that of seed germination under all storage conditions. The oxygen-sensing test reliably and rapidly assessed seed quality of G. biloba. The germination rate of Zhejiang Dafoshou was accurately predicted by T (GT).
基金The research was supported by National Natural Science Foundation of China (30371154).
文摘The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis （2-DE）. The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pIr.8, 31kD/pIr.8, 29kD/pIr.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins （VSPs）.
基金Basic Operating Expenses of Guangxi Academy of Agricultural Sciences(GNK 2015YT98,GNK 2020YM21,GNK 2020YM20)。
文摘In order to explore the best storage temperature and storage period of dragon fruit seeds,with the constant-humidity germplasm cold storage of Guangxi Academy of Agricultural Sciences as a platform and storage temperature as an environmental factor,the germination characteristics of dragon fruit seeds and seedling growth characteristics under low temperature storage(8,4,-1,-10℃)and room temperature storage(25-30℃)over time were explored.The results showed that with the extension of the storage time,the seed germination rates,germination index,healthy seedling rates,seedling heights and fresh weights of 10 seedlings of the three dragon fruit varieties showed basically the same trends,showing a downward trend.After 367 d of cold storage,the germination rate decreased from 100%to 92.33%-98.00%,and the room temperature treatments decreased to 54.00%-86.30%;and the healthy seedling rates of cold-stored seeds decreased from 96.33%-98.00%to 77.33%-97.3%,and the values of the room temperature treatments dropped to 12.00%-68.33%.The germination rates,germination index,healthy seedling rates and seedling heights of the three dragon fruit varieties stored at low temperatures were basically significantly higher than those of the room temperature treatments within 367 d of storage.The germination rates of the three varieties stored at 8℃had no significant differences from other low temperature treatments,but the germination index was significantly lower than other low temperature treatments at 367 d.It showed that low temperature storage at-10-8℃could significantly prolong the vigor and storage time of dragon fruit seeds.Considering cost and other factors,it is better to store dragon fruit seeds in a dry environment at 4℃.The seeds stored at 4℃for 1 year showed a germination rate maintained over 90%and a healthy seedling rate over 85%.
