Optimal Method of Extraction Process of Flavonoids from Ginkgo biloba

[Objectives] To optimize the extraction of total flavone in folium Ginkgo biloba leaves.[Methods] Using the ultrasonic extraction method, the three levels of maximum impact were selected by each factor selection, and the optimal extraction technology was obtained by box-benhnken test design analysis.[Results] After analyzing the results, the optimal process was as follows: a time of 28.89 min and liquid/material ratio of 31.38∶ 1, the temperature was 40.74℃. In this way, NaNO 2-Al (NO 3) 3-NaOH was used as color developing agent, and the theoretical extraction of flavone in ginkgo leaf can reach 11.74 mg/g.[Conclusions] This method can be used to extract total flavonoids from G. biloba leaves with simple operation, low cost and good repeatability.

Effects of Ginkgo Leaf Extract on Function of Dendritic Cells and Th1/Th2 Cytokines in Patients with Unstable Angina Pectoris

Objective： To investigate the effects of Ginkgo leaf extract （GLE） on function of dendritic cells （DO） and Th1/Th2 cytokines in patients with unstable angina pectoris（UAP）. Methods： Fifty-four patients with UAP were equally assigned into two groups, the treated group and the control group, both treated with conventional Western medicine, but with GLE given additionally to the treated group. Blood of all patients was taken before and 4 weeks after treatment to prepare the peripheral mononuclear cells, then which were incubated in the completed medium containing granulocyte-macrophage colony stimulatory factor （GMCSF） and interleukin-4 （IL-4） to induce mature DO. The expression of co-stimulating factor CD86 （B7-2） on the surface of DC was detected by flow cytometry, and the stimulating capacity of DC was determined by mixed lymphocyte reaction （MLR）. The blood levels of cytokines, interferon-γ （IFN-γ）, and IL-4, were analyzed by ELISA, and blood C-reactive protein （CRP） level by turbidimetry. Moreover, the direct effect of Ginkgolide B on CD86 expression on DO were also tested in vitro. Results： After treatment, CD86 expression on DO, the stimulating capacity of DO as well as levels of IFN-γ and ORP were lowered in both groups （P〈0.05 or P〈0.01）, but the changes were much more significant in the treated group than those in the control group. Ginkgolide B showed a direct inhibitory effect on the CD86 expression on DO. Conclusion： The inhibition of GLE on DO and thereby the suppression on inflammatory reaction may be one of the mechanisms of GLE in treating patients with UAP.

Ginkgo Leaf Extract and Armillariella Mellea Powders Oral(银杏蜜环口服溶液) attenuates inflammation of microglia in habenular nucleus through CX3CL1-CX3CR1 axis in post stroke depression

OBJECTIVE The Ginkgo Leaf Extract and Armillariella Mellea Powders Oral(Yinxingmihuan Koufu Rongye,YXMH),a representative drug for"Treating both Brain and Heart",showed considerable clinical effects in isch⁃emic cardiovascular and cerebral vascular diseases.Recently,it is reported that YXMH has the potential for treating myocardial and cerebral ischemia related mental disorders,such as post stroke depression(PSD)and chronic heart disease(CHD)associated anxiety disorder.However,its mechanism has not been clearly elucidated.Meanwhile,increasing evidence revealed that there are close functional links between depression and habenular nucleus.The present study investigates the underlying mechanism of YXMH on attenuating the inflammation of microglia in habenular nucleus through CX3CL1-CX3CR1 axis in in a rat model of PSD.METHODS Rats were randomly devided into sham group,model group,Ginaton group(18 mg·kg^-1),Armillariella Mellea group(600 mg·kg-1),Fluoxetine group(10 mg·kg^-1),YXMH high-dose group(618 mg·kg^-1)and YXMH low-dose group(309 mg·kg^-1).The PSD model was induced by transarterial microembolization combined with sleep deprivation(2-Chloro-D-phenylalanine,PCPA,IH,200 mg·kg^-1,for 3 times,before the behavior test)in SD male rats.Then rats were treated with corresponding medicaments through gavage once a day until 3 weeks later,followed by body mass measurement,neurological deficit score evaluation,gripping strength and thermal withdrawl latency measurement,as well as depression related behavioral indicators,the open field test(OFT)and sucrose preference test.The pathological morphological changes of habenular nucleus was observed by HE staining,the expression of IBA-1 was measured and analyzed by immunohistochemistry staining,and alterations of proteins and genes related to the CX3CL1-CX3CR1 axis were analyzed using Western blotting(CX3CL1,CX3CR1)and real-time polymerase chain reaction(PCR)(CX3CL1,CX3CR1).RESULTS Compared with the sham group,rats in the model group manifested as decreased body mass,deficient neurological behavior and gripping strength,reduced loco⁃motor activity and sugar water consumption,as well as elevated thermal withdrawl latency(P<0.05,P<0.01).Mean⁃while,the pathological morphology of the habenular nucleus on the ischemic hemisphere showed significant neuronal degeneration,microglial proliferation,inflammatory cells and glia cells infiltration,together with up-regualted expression of IBA-1,CX3CL1,CX3CR1 protein and CX3CL1,CX3CR1 mRNA.YXMH attenuated inflammation of microglia in habenular nucleus through improving pathological morphology,inhibiting IBA-1 activation,down-regulating the expres⁃sion of CX3CL1 and CX3CR1 proteins and genes,and thus improved the behavior performance of ischemic injury and depression.CONCLUSION YXMH ameliorates neurological deficit and depressive behavior in rat model of PSD induced by transarterial microembolization combined with sleep deprivation,and the mechanism is probably related to attenu⁃ating inflammation of microglia in habenular nucleus through CX3CL1-CX3CR1 axis.

Effect of Ginkgo Leaf Extract on Vascular Endothelial Function in Patients with Early Stage Diabetic Nephropathy

Objective:To explore the effect of ginkgo leaf extract(GLE) on vascular endothelial function(VEF) in patients with early stage diabetic nephropathy(DN).Methods:Sixty-four patients were randomized equally by a randomzing digital table into two groups,the treated group and the control group.They were all treated for 8 weeks with conventional therapy for diabetes,but GLE tablets were given to the treated group additionally. Changes in VEF were estimated before and after treatment by ultrasonic examination of t...

Ginkgo Leaf Extract and Dipyridamole Injection as Adjuvant Treatment for Angina Pectoris:A Meta-Analysis of 41 Randomized Controlled Trials

Objective： To provide information about the effectiveness and safety of Ginkgo Leaf Extract and Dipyridamole Injection （GD） as one adjuvant therapy for treating angina pectoris （AP） and to evaluate the relevant randomized controlled trials （RCTs） with meta-analysis. Methods： RCTs concerning AP treated by GD were searched in China Biology Medicine Disc （SinoMed）, PubMed, the China National Knowledge Infrastructure Database （CNKI）, the Chinese Scientific Journals Database （VIP）, Wanfang Database, Embase, and the Cochrane Library, from inception to February, 2017. The Cochrane Risk Assessment Tool was adopted to assess the methodological quality of the RCTs. The Review Manager 5.3 software was utilized to conduct the meta-analysis. Results： A total of 41 RCTs involving 4,462 patients were included in the meta-analysis. The results indicated that the combined use of GD and Western medicine （WM） against AP was associated with a higher total effective rate [risk ratio （RR）=1.25, 95% confidence interval （CI）： 1.21-1.29, P〈0.01], total effective rate of electrocardiogram （RR=1.29, 95% CI： 1.21-1.36, P〈0.01）. Additional, GD combined with WM could decrease the level of plasma viscosity [mean difference （MD）=-0.56, 95% CI： -0,81 to -0.30, P〈0.01], flbrinogen [MD=-1.02, 95% CI： -1.50 to -0.54, P〈0.01], whole blood low shear viscosity [MD=-2.27, 95% CI： -3.04 to -1.49, P〈0.01], and whole blood high shear viscosity （MD=-0.90, 95% CI： 1.37 to -0.44, P〈0.01）. Conclusions： Comparing with receiving WM only, the combine use of GD and WM was associated with a better curative effect for patients with AP. Nevertheless, limited by the methodological quality of included RCTs more large-sample, multi-center RCTs were needed to confirm our findings and provide further evidence for the clinical utility of GD.

Protective Effect of Ginkgo Biloba Leaf Extract on Learning and Memory Deficit Induced by Aluminum in Model Rats

Objective： To examine the protective effect of Ginkgo biloba leaf extract （GbE） on learning and memory deficit induced by aluminum chloride （AlCl3）, and explore its mechanisms. Methods： The rat models with learning and memory deficit were induced by administering via gastrogavage and drinking of AlCl3 solution. And the model rats were treated with GbE at the dose of 50, 100, 200 mg/kg every day for 2 months accompanied with drinking of AlCl3 solution, respectively. Their abilities of spatial learning and memory were tested by Morris water maze, and the acetylcholinesterase （ACHE） activity in serum was assayed with chemical method, the AChE expression in hippocampus was observed by immunohistochemistry assay, and then quantitative analysis was done by BI 2000 image analysis system. Results： Learning and memory deficit of rats could be induced by AlCl3 solution （P〈0.01）, and AChE expressions in rats hippocampus were increased （P〈0.01）; GbE ameliorated learning and memory deficit and reduced AChE expression in rats hippocampus in a dose-dependent manner, while GbE significantly increased serum AChE activity at the dose of 200 mg/kg each day （P〈0.05）. Conclusion： GbE can ameliorate learning and memory deficit induced by AlCl3, which may be due to its inhibition of the AChE expression in hippocampus.

Ginkgo biloba leaf extract effects on inducible nitric oxide synthase, Bcl-2, and Bax expression in rat models of spinal cord injury

BACKGROUND：Ginkgo biloba leaf extract exhibits neuroprotective effects in spinal cord injury. However, the mechanisms of action remain unclear. OBJECTIVE： To investigate inducible nitric oxide synthase （iNOS） and Bcl-2/Bax expression in the injured spinal cord, and to explore the neuroprotective mechanisms of ginkgo biloba leaf extract in rats with spinal cord injury. DESIGN, TIME AND SETTING： The randomized, controlled, cell molecular biology experiment was performed at Soochow University, China from March 2007 to March 2008. MATERIALS： A total of 120 healthy, adult Sprague Dawley rats were selected for this study. Rat models of moderate acute thoracic （T9） spinal cord injury were established using the modified Allen method. Shuxuening injection was obtained from Zhenbaodao Pharmaceutical Co., Ltd., China. Methylprednisolone was purchased from North China Pharmaceutical Co., Ltd. METHODS： All rats were equally and randomly divided into four groups. Only the spinal cord was exposed in the sham operation group rats. In the trauma group, rats were not treated with drugs following spinal cord injury. Rats in the hormone group were intraperitoneally injected with 30 mg/kg methylprednisolone following spinal cord injury. Rats in the ginkgo biloba leaf extract group were intraperitoneally infused with a 1.0 mL/kg Shuxuening injection per day. MAIN OUTCOME MEASURES： At 1 hour, as well as 1, 3, 5, 7, and 14 days after spinal cord injury, iNOS- and Bcl-2/Bax-positive cells were quantified with immunohistochemistry. Pathological changes were detected using hematoxylineosin staining under an optical microscope. RESULTS： Spinal cord injury in the ginkgo biloba leaf extract and hormone groups was milder compared with the trauma group. Demyelination was significantly ameliorated and the necrotic cavity was obviously reduced in the injured spinal cord of rats in the ginkgo biloba leaf extract and hormone groups at each time point. iNOS expression was increased in the injured spinal cord, and reached a peak at 5 days. The number of iNOS-positive cells was lower in the ginkgo biloba leaf extract and hormone groups compared with the trauma group （P 〈 0.05-0.01）. The number of iNOS-positive cells was lower in the ginkgo biloba leaf extract group compared with the hormone group at 7 and 14 days after spinal cord injury （P 〈 0.05）. Bcl-2 expression reached a peak at 3 days, and Bax expression reached a peak at 5 days following rat spinal cord injury. Bcl-2 expression was increased, but Bax expression was decreased in the ginkgo biloba leaf extract and hormone groups compared with the trauma group （P 〈 0.05-0.01）. Bcl-2 expression was greater, but Bax expression was reduced in the ginkgo biloba leaf extract group compared with the hormone group at 7 and 14 days after spinal cord injury （P 〈 0.05）. CONCLUSION： Ginkgo biloba leaf extract exhibits neuroprotective effects by upregulating Bcl-2 expression, downregulating Bax expression, and significantly inhibiting high expressions of iNOS in the injured spinal cord. The neuroprotective effects of ginkgo biloba leaf extract are greater compared with methylprednisolone at 1 week after spinal cord injury.

Determination of the Flavonoids from Ginkgo Biloba Extract by High Performance Liquid Chromatography

HPLC method for analysis of the flavonoids from ginkgo biloba extract (GBE) was studied. By suitable selection of columns. symmetrical chromatographic peaks were obtained without using acidic modifier in the mobile phase, which can eliminate the time for cleaning the chromatographic system and simplify the analystic method for GBE Experimental conditions: column: Hypersil BDS C-18, 5mumx4x250 mm: column temperature: 35degreesC; mobile phase: 46% methanol-54% water; flow rate: 0.7 mL/min; detection wavelength: 360nm.

银杏叶茶总黄酮含量测定及抗氧化活性研究

建立紫外-可见分光光度法测定不同加工过程银杏叶茶样品中总黄酮含量,通过碘还原试验和DPPH自由基清除法评估银杏叶茶提取物的抗氧化活性。结果显示,处于采摘、凋青、揉青、烘焙四种不同生产工艺过程中银杏叶茶样品中总黄酮含量最高可达42.58 mg/g。以上四种银杏叶茶提取物不仅具有适中的还原碘能力,还具有较强的清除DPPH自由基能力,浓度为10 mg/mL时的DPPH自由基清除率分别为90.48%、90.04%、82.57%、79.78%。研究结果为科学控制银杏叶茶的质量提供参考数据。

银杏叶提取物联合前列地尔对慢性阻塞性肺疾病合并肺动脉高压患者的临床疗效

目的探讨银杏叶提取物联合前列地尔对慢性阻塞性肺疾病合并肺动脉高压患者的临床疗效。方法122例患者随机分为对照组和观察组,每组61例,对照组给予前列地尔,观察组在对照组基础上加用银杏叶提取物,疗程3个月。检测临床疗效、肺功能指标(FVC、FEV_(1)、FEV_(1)/FVC)、血气指标(PaO_(2)、PaCO_(2))、PASP、mPAP、IL-6、ET-1、hs-CRP、NO、右心功能指标(RVEF、RVESD)、不良反应发生率变化。结果观察组总有效率高于对照组(P<0.05)。治疗后,2组肺功能指标、PaO_(2)、RVESD升高(P<0.05),PaCO_(2)、IL-6、ET-1、hs-CRP、NO、RVEF降低(P<0.05),以观察组更明显(P<0.05)。2组不良反应发生率比较,差异无统计学意义(P>0.05)。结论银杏叶提取物联合前列地尔可安全有效地改善慢性阻塞性肺疾病合并肺动脉高压患者肺功能和右心功能,减轻炎症反应。

银杏落叶色素染料提取工艺的优化及染色应用

为了探索银杏落叶中色素染料的提取工艺及在棉织物染色中的应用.利用单因素试验和正交试验法探明银杏叶色素提取的最佳工艺,通过测试染色后棉织物的K/S值分析不同酸碱度条件下对棉织物染色效果的影响.结果表明,银杏落叶中色素染料提取的最佳工艺条件为:料液比1∶10,乙醇体积分数80%,提取温度80℃,提取时间60 min.在pH值为3、5、7时染色棉织物K/S值都高于pH值为9、11时的染色棉织物K/S值,也就是说在酸性和中性条件下银杏落叶色素染料对棉织物的染色效果优于在碱性条件下的染色效果.

复方苁蓉益智胶囊治疗轻中度血管性痴呆

目的探讨复方苁蓉益智胶囊治疗轻中度血管性痴呆的临床疗效及对患者各临床检测指标的影响。方法选择2022年9月-2023年5月收治的轻中度血管性痴呆患者140例,随机分为对照与治疗组,各70例。对照组给予银杏叶提取物注射液治疗,治疗组在对照组基础上联合复方苁蓉益智胶囊治疗。比较2组临床疗效结果,治疗前后认知、神经功能、精神行为症状、血液流变学、血流动力学、应激反应指标、血清血管内皮生长因子(VEGF)、脑源性神经营养因子(BDNF)、基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶-2(MMP-2)水平变化,比较2组不良反应发生情况。结果治疗组治疗6个月后临床疗效总有效率(95.71%,67/70)高于对照组(78.57%,55/70)(P<0.05)。2组治疗6个月后MMSE评分、大脑前动脉平均血流速度、中动脉平均血流速度及血清SOD、VEGF、BDNF水平高于治疗前(P<0.05),治疗组高于对照组(P<0.05);2组治疗6个月后NIHSS、NPI-Q评分、全血低切黏度、全血高切黏度、血浆黏度及血清MDA、Caspase-12、CRP78、MMP-9、MMP-2水平代于治疗前(P<0.05),治疗组低于对照组(P<0.05)。治疗组治疗不良反应总发生率低于对照组(P<0.05)。结论复方苁蓉益智胶囊治疗轻中度血管性痴呆患者临床疗效较好,安全性较高。

琥珀酸美托洛尔联合银杏达莫注射液对冠心病心绞痛患者心率变异性、血液流变学的影响

目的探讨银杏达莫注射液联合琥珀酸美托洛尔对冠心病心绞痛患者水平的影响。方法选取济南市第四人民医院2019年7月-2021年7月收治的冠心病心绞痛患者80例,随机数字表法将其分为对照组(38例)和观察组(42例)。对照组采用银杏达莫注射液治疗;观察组在对照组基础上给予琥珀酸美托洛尔治疗。比较2组治疗前后心绞痛发作次数与持续时间、血清炎症因子水平、24 h动态血压、心率变异性(heart rate variabiliby,HRV)、血液流变学指标;比较治疗后2组临床治疗效果、不良反应。结果治疗后,观察组心绞痛发作次数、持续时间、6 min步行测试距离与对照组相比较,发作次数持续较短、距离较长(P<0.05);治疗后,2组血清肿瘤坏死因子α(TNF-α)、细胞间黏附分子-1(ICAM-1)、C-反应蛋白(CRP)水平相比均低于治疗前,HRV比较均高于治疗前,且观察组明显优于对照组(P<0.05);治疗后,2组的24 h动态血压、纤维蛋白原、血浆黏度、全血低切黏度、全血高切黏度差值相比均低于治疗前,且观察组均低于对照组,差异具有统计学意义(P<0.05)。结论经琥珀酸美托洛尔联合银杏达莫注射液对早期冠心病心绞痛患者进行治疗后,降低患者血黏度,减少患者的发作次数与持续时间,HRV均升高,血液流变学指标、24 h动态血压水平均降低,血清炎症因子得到抑制,防止病情进一步的发展,临床效果较为显著。

银杏达莫注射液联合丁苯酞治疗急性脑梗死的临床效果

目的探讨急性脑梗死(ACI)患者采用银杏达莫注射液联合丁苯酞治疗的临床效果。方法选取2021年11月至2022年3月在稷山县人民医院接受治疗的ACI患者共计92例,以随机数表法分为对照组与研究组,各46例,对照组给予丁苯酞治疗,研究组给予银杏达莫注射液联合丁苯酞治疗,对两组临床疗效、神经功能缺损、血流速度指标、血液流变学指标、功能恢复结局、不良反应进行比较。结果研究组治疗有效率(95.65%)较对照组(80.43%)更高,差异有统计学意义(P<0.05);两组治疗后与同组治疗前相比,美国国立卫生院神经功能缺损评分(NIHSS)均降低,且研究组较对照组更低,差异有统计学意义(P<0.05);两组治疗后与同组治疗前相比,舒张末期血流速度(Vd)、平均血流速度(Vm)、收缩期峰值血流速度(Vs)均加快,研究组较对照组更快,差异有统计学意义(P<0.05);两组治疗后与同组治疗前相比,血浆粘度(PSV)、全血高切粘度(HSV)、纤维蛋白原(FIB)降低,研究组较对照组更低,差异有统计学意义(P<0.05);随访6个月与同组治疗前,两组改良Rankin量表(mRS)评分降低,研究组较对照组更低(P<0.05),差异均有统计学意义;研究组不良反应发生率(4.35%)较对照组(23.91%)更低,差异有统计学意义(P<0.05)。结论ACI患者采用银杏达莫注射液联合丁苯酞治疗,能够促进临床疗效提升,改善神经功能、血流速度,促进血液流变学指标改善,减少不良反应发生。

银杏叶提取物-壳聚糖/聚乙烯醇复合膜对冷鲜牛肉保鲜效果的影响

为开发具备优质保鲜效果的复合膜,该试验将银杏叶提取物(Ginkgo biloba leaf extract,GBLE)作为保鲜剂加入到壳聚糖/聚乙烯醇(chitosan/polyvinyl alcohol,CS/PVA)复合膜中,研究不同添加量的GBLE(2%、6%和10%,质量分数)对复合膜性能的影响,并应用到冷鲜牛肉保鲜中。结果表明,GBLE能显著提高复合膜的拉伸强度、不透明度和抗氧化活性(P<0.05),显著降低复合膜的断裂伸长率、水蒸气透过率(P<0.05)。在12 d的保鲜试验中,与对照组相比,GBLE-CS/PVA复合膜组冷鲜牛肉的感官评分更高,且能有效延缓冷鲜牛肉的pH值、菌落总数、挥发性盐基氮含量以及硫代巴比妥酸值的上升。因此,GBLE-CS/PVA复合膜在冷鲜牛肉贮藏保鲜方面具有应用前景。

基于近红外光谱一致性检验模型鉴别银杏叶提取物片

目的 利用近红外光谱(NIR)法建立进口药品银杏叶提取物片的一致性检验模型。方法 通过对国外A企业生产的46批银杏叶提取物片进行光谱采集,运用OPUS7.8光谱分析软件对建模光谱图进行二阶导数和矢量归一化预处理,并采用国产不同企业同规格银杏叶片验证模型,建立银杏叶提取物片的近红外一致性检验模型。结果 建立的国外A企业样品近红外一致性模型能鉴别该厂家生产的银杏叶提取物片。结论 利用一致性检验方法,成功建立了国外A企业生产的银杏叶提取物片一致性检验模型,适用于该品牌银杏叶提取物片快速筛查。

银杏叶提取物注射液联合阿托伐他汀对老年急性脑梗死患者脂质水平、炎症反应及血栓弹力图监测指标的影响

目的观察银杏叶提取物注射液联合阿托伐他汀对老年急性脑梗死患者脂质水平、炎症反应及血栓弹力图监测指标的影响。方法前瞻性选取2021年3月至2023年12月就诊于安徽医科大学第一附属医院东区的80例老年急性脑梗死患者作为研究对象。按照随机数字表法将入组患者分为他汀类组和联合组,每组各40例。两组均给予对症治疗,他汀类组加用阿托伐他汀治疗,联合组在他汀类组基础上给予银杏叶提取物注射液治疗。检测并比较两组患者治疗前及治疗10 d后的脂代谢[总胆固醇、甘油三酯、低密度胆固醇(LDL-C)、高密度胆固醇(HDL-C)]、趋化因子[血清趋化因子fractalkine(FKN)、CX3C趋化因子受体1(CX3CR1)]、炎症因子[白细胞介素1β(IL-1β)、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)]、血栓弹力图[凝血反应时间(R值)、血液凝固时间(K值)、凝血块形成曲线的最宽距离(MA值)、凝固角]及美国国立卫生研究院卒中量表(NIHSS)、改良Rankin量表(mRS)变化。结果治疗10 d后,两组总胆固醇、甘油三酯、LDL-C均较治疗前降低,HDL-C均较治疗前升高,且联合组的总胆固醇、甘油三酯、LDL-C水平分别为(3.45±0.69)、(1.77±0.54)、(2.11±0.38)mmol/L,均低于他汀类组[(3.89±0.78)、(2.03±0.59)、(2.34±0.45)mmol/L],HDL-C水平为(1.74±0.34)mmol/L,高于他汀类组[(1.57±0.33)mmol/L],差异均有统计学意义(P<0.05)。治疗10 d后,两组FKN、CX3CR1水平均较治疗前降低,联合组的FKN、CX3CR1水平分别为(1.67±0.48)、(5.17±1.64)μg/L,均低于他汀类组[(2.12±0.56)、(6.05±1.78)μg/L],差异均有统计学意义(P<0.05)。治疗10 d后,两组IL-1β、CRP、TNF-α水平均较治疗前降低,联合组的IL-1β、CRP、TNF-α水平分别为(6.07±1.22)pg/mL、(8.89±1.44)mg/L、(13.08±2.87)pg/mL,均低于他汀类组[(7.45±1.74)pg/mL、(11.56±2.05)mg/L、(17.41±3.22)pg/mL],差异均有统计学意义(P<0.05)。治疗10 d后,两组NIHSS评分、mRS评分均较治疗前降低,联合组的NIHSS评分、mRS评分分别为(11.74±1.28)、(2.43±0.25)分,均低于他汀类组[(14.02±1.32)、(2.92±0.31)分],差异均有统计学意义(P<0.05)。治疗10 d后,两组R值、K值升高,MA值、α角均较治疗前降低,差异均有统计学意义(P<0.05);两组治疗10 d后血栓弹力图指标比较,差异无统计学意义(P>0.05)。结论银杏叶提取物注射液联合阿托伐他汀可调节老年急性脑梗死患者脂代谢,降低FKN、CX3CR1的表达,抑制炎症反应,但在改善凝血功能方面影响有限。

银杏达莫注射液联合倍他司汀治疗后循环缺血眩晕的疗效分析

目的分析后循环缺血眩晕患者采用银杏达莫注射液联合倍他司汀治疗的临床效果。方法选取78例后循环缺血眩晕患者,按照随机数字表法分为实验组(银杏达莫注射液联合倍他司汀治疗)与对照组(倍他司汀治疗),各39例。对比两组患者临床疗效、眩晕症评分、血液粘度以及不良反应发生率。结果实验组治疗总有效率为100.00%,高于对照组的89.74%,差异显著(P<0.05)。治疗前,两组患者眩晕症状评分简化量表(VSS-SF)、眩晕障碍量表(DHI)、Berg平衡量表(BBS)评分对比无差异(P>0.05);治疗后,实验组VSS-SF、DHI、BBS评分分别为(8.22±1.04)、(10.49±1.22)、(50.49±1.22)分,均优于对照组的(12.49±1.28)、(15.67±1.45)、(35.28±1.14)分,差异显著(P<0.05)。治疗前,两组患者全血高切粘度、全血中切粘度、全血低切粘度对比无差异(P>0.05);治疗后,实验组全血高切粘度(4.57±0.49)mPa·s、全血中切粘度(5.65±0.42)mPa·s、全血低切粘度(8.62±1.31)mPa·s均低于对照组的(4.92±0.58)、(5.96±0.75)、(9.27±1.54)mPa·s,差异显著(P<0.05)。两组不良反应发生率对比无差异(P>0.05)。结论后循环缺血眩晕采取银杏达莫注射液与倍他司汀共同治疗效果明显,可明显缓解眩晕症状,改善血液粘度,是一种安全有效的治疗方法。

银杏达莫联合依达拉奉治疗急性缺血性脑卒中的效果及对血流动力学的影响

目的探讨银杏达莫联合依达拉奉治疗急性缺血性脑卒中(AIS)的效果及对血流动力学的影响。方法选取2021年3月至2022年3月收治的60例AIS患者为研究对象,根据随机数字表法将其分为对照组和研究组,各30例。对照组接受依达拉奉治疗,研究组在对照组基础上加用银杏达莫治疗。比较两组的治疗效果。结果研究组的治疗总有效率高于对照组(P<0.05)。治疗后,两组的血浆黏度、血小板聚集指数、大脑中动脉血管搏动指数(PI)均降低,大脑中动脉收缩期血流速度(PSV)、舒张末期血流速度(EDV)均升高,且研究组优于对照组(P<0.05)。治疗后,两组的基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)水平均降低,B淋巴细胞瘤-2(Bcl-2)、内皮生长因子受体-1(FLK-1)水平均升高,且研究组优于对照组(P<0.05)。治疗后,两组的美国国立卫生院卒中量表(NIHSS)评分均降低,且研究组低于对照组(P<0.05)。结论银杏达莫联合依达拉奉能调节AIS患者血流动力学及血清指标,减轻神经功能损伤,利于病情转归,值得在临床中推广及应用。

银杏蜜环口服溶液治疗冠心病合并抑郁的临床效果及对免疫功能的影响

目的 探讨银杏蜜环口服溶液治疗冠心病合并抑郁的临床效果及对免疫功能的影响。方法 选取2019年7月至2023年7月金华市中医医院和慈溪市第七人民医院收治的冠心病合并抑郁患者124例,随机将其分为对照组和观察组,每组各62例。对照组患者采用常规方案治疗,观察组患者在此基础上增加银杏蜜环口服溶液治疗。经8周治疗后,比较两组患者的炎症因子、免疫功能、生活质量及抑郁评分。结果 治疗后,两组患者的C反应蛋白(C-reactive protein,CRP)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-8(interleukin-8,IL-8)、CD8^(+)水平均显著低于本组治疗前,CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)均显著高于本组治疗前(P<0.05);观察组患者的CRP、TNF-α、IL-8、CD8^(+)水平均显著低于对照组,CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)均显著高于对照组(P<0.05)。治疗后,两组患者的西雅图心绞痛量表(Seattle angina questionnair,SAQ)各维度评分均显著高于本组治疗前(P<0.05),观察组患者的SAQ各维度评分均显著高于对照组(P<0.05)。治疗后,观察组患者的汉密尔顿抑郁量表评分显著低于对照组[(9.26±1.58)分vs.(12.87±1.89)分,t=11.538,P<0.001]。结论 银杏蜜环口服溶液可抑制冠心病合并抑郁患者的炎症反应并改善其抑郁症状,提高患者的免疫功能和生活质量。