Optimization of Ultrasonic-Assisted Extraction Process of Polysaccharides from American Ginseng and Evaluation of Its Immunostimulating Activity

Ultrasonic-assisted extraction （UAE） of American ginseng polysaccharides （AGP） was investigated using response surface methodology. Three-factor-three-level Box-Behnken design was employed to optimize the ultrasonic power, extraction time and ratio of water to raw material to obtain a high AGP yield. The analysis of variance and response surface plots indicated that ultrasonic power was the most important factor affecting the extraction yield. The optimal conditions were ultrasonic power 400 W, extraction time 71 min, and ratio of water to raw material 33 mL g-1. Under these conditions, the yield of AGP was 8.09%, which was agreed closely to the predicted value. Gas chromatography （GC） analysis showed that AGP was composed of arabinose, rhamnose, galactose, glucose, and galacturonic acid. Fourier transform infrared spectra revealed the general characteristic absorption peaks of AGP. In addition, AGP exhibited good immunostimulating activities by up-regulating the production of nitric oxide and cytokines. Compared with hot water extraction, UAE required shorter extraction time and gave a higher extraction yield, without changing the structure and immunostimulating activity of AGP. The results indicated that UAE could be an effective and advisable technique for the large scale production of plant polysaccharides.

Anticancer Effect of Ginseng Leaves Crude Polysaccharides on Human Hepatoma Cell SMMC-7721

Background: Polysaccharides extracted from the medicinal herbs are known to possess antitumor effects. Although there is a significant number of evidences implicated on the beneficial effects of the ginseng leaves ginsenoside with diverse associated mechanisms, reports on the anticancer by the ginseng leaves crude polysaccharides (GLCP) are not sufficient. Experiments were carried out to unravel the anticancer effects of GLCP. Methods: Cells were treated with GLCP (0.5 - 2 mg/ml) for 48h. MTT method was used to detect the cell viability. Western blot and flow cytometry were used to detect apoptotic rate. Western blot and acridine orange staining were used to detect the cell autophagy. Results: Compared with the normal human liver cell (Chang liver), GLCP (1.5 - 2 mg/ml) significantly reduction cell viability, promote apoptosis-related proteins expression, promote cell apoptosis and autophagy in SMMC-7721 cells. But caveolin-1 gene silencing could inhibit the anticancer effect of GLCP. Conclusions: These data suggest that GLCP promote autophagy and apoptosis in human hepatoma cell SMMC-7721. We speculate that its mechanism may be associated with the caveolin-1 which is an essential structural molecule of caveolae. Although the effect of GLCP inhibited of liver cancer is not very strong, we are more interested in the GLCP which plays a tumor suppressor role in health care.

Clinical potentials of ginseng polysaccharide for treating gestational diabetes mellitus

Gestational diabetes mellitus(GDM)is the most common glucose metabolism complication or cause of potential impaired glucose tolerance that can occur either before or during pregnancy and lactation.The prevalence of GDM and its related complications in young women is increasing,and this condition may cause serious outcomes and health hazards to the foetus.However,traditional oral hypoglycaemic drugs have potential safety hazards;therefore,it is urgent to develop new,safe,effective,and easily administered agents and remedies.Ginseng polysaccharide(GPS),which is isolated from Panax(P.)ginseng C.A.Meyer,exhibits notably promising biological activities and effects;specifically,it has been shown to lower blood glucose with mild,safe,and nontoxic characteristics,and it can also improve human bodily functions.Hence,we hypothesise that GPS might be used as an additional therapy and candidate agent for treating GDM.This review innovatively summarizes the available reports and evidence from basic studies to analyze the potential for and feasibility of using GPS as a new therapeutic agent for treating GDM.Additionally,for the first time,this review provides a rationale for the use of GPS.Our summarized results show that GPS may be developed as a novel antidiabetic drug and a remedy for use in preventing and treating GDM,with great application prospects.

Total Fractionation and Analysis of Polysaccharides from Leaves of Panax ginseng C.A. Meyer

The water-soluble polysaccharides isolated from the leaves of Panax ginseng C. A. Meyer were totally fractionated into one neutral fraction（GLPN-1）, six acidic fractions（GLPN-2, GLPA-1a, GLPA-1b, GLPA-1c, GLPA-2 and GLPA-3） by a combination of ethanol precipitation, ion-exchange and gel permeation chromatography. All the fractions were analyzed by determining monosaccharide composition, molecular weight distribution. The resuits show that GLPN-1 was mainly composed of Gal（38.1%） and Ara（33.6%） and presumed to be an arabinogalactan. GLPA-1a, GLPA-1b and GLPA-2 mainly contained Gal, Ara, Rha and GalA. The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%. The molar ratios of Rha/GalA in GLPA-1a, GLPA-1b and GLPA-2 were 1：1, 1：2 and 1：2.7, respectively, indicating that they belong to type-1 rhamnogalacturonan （RG-1）-rich pectin. GLPA-1c was mainly composed of GalA（78%）, belonging to homogalacturonan（HG）-rich pectin. GLPA-3 might be a glycoprotein.

Physicochemical properties and antioxidant activities of polysaccharides extracted from Panax ginseng C.A.Meyer with graded percipitation method

Ginseng polysaccharides were extracted by water decoction from Panax ginseng C.A.Meyer(Cultivated Ginseng),named CGPS.Four polysaccharide fractions,CGPS-20,CGPS-40,CGPS-60 and CGPS-80,were precipitated at final ethanol concentrations of 20%,40%,60%and 80%,respectively.Physicochemical properties,molecular weight,monosaccharide composition and antioxidant capacity of polysaccharide fractions were all investigated.The results indicated that changing the concentration of ethanol could precipitate polysaccharides into fractions with different molecular weights,functional group composition and physicochemical properties,eventually leading to differences in antioxidant activity,which would help to find a simple,efficient,and reliable method for rapid extraction and purification of antioxidant polysaccharides from Panax ginseng C.A.Meyer.Among the four polysaccharide fractions,CGPS-80 had lower molecular weight,higher contents of uronic acid and total phenolic,and stronger scavenging ability on DPPH∙and ABTS∙+radicals.

甘草残渣中多糖GPS抗肿瘤作用的研究

自甘草残渣中分离一种水溶性葡聚糖 GPS,急性毒性实验证明其属无毒物质 .通过体内抑瘤实验表明 ,GPS对小鼠肉瘤 S-1 80及艾氏腹水移植瘤皆有明显抑制作用 ,其抑制率分别高于 80 %和 30 % .

不同分子量黑参多糖对皮肤保湿及光损伤保护作用

以黑参多糖为研究对象,分析两种黑参多糖HSP-1和HSP-2的结构,研究二者对皮肤保湿以及光损伤保护作用的效果。结果表明,HSP-1和HSP-2均含有蛋白质、均具有多糖特征峰,一级结构一致,得率HSP-1<HSP-2,分子量HSP-1<30 kDa、HSP-2>30 kDa。在单糖构成上,HSP-1以半乳糖和鼠李糖为主要单糖成分,HSP-2以阿拉伯糖为主要单糖成分。HSP-1具有三螺旋结构、HSP-2不具有三螺旋结构,二者的空间结构不一致。通过对比吸湿能力、保湿能力以及小鼠皮肤的厚度色度、羟脯氨酸、糖胺多糖和脂质含量以及总抗氧化能力、超氧化物歧化酶和丙二醛含量,发现HSP-1的吸湿保湿、抑制皮肤羟脯氨酸和脂质流失、糖胺多糖累积以及减缓氧化应激产生的炎症状态的能力均优于HSP-2。因此HSP-1多糖对皮肤保湿及光损伤保护的作用效果较佳。

人参白术多糖复合物对免疫抑制模型小鼠的免疫功能影响

试验旨在研究人参多糖与白术多糖复合使用对小鼠免疫功能的影响。将300只体重接近的小鼠随机分为10组,其中T1~T5组为免疫抑制模型组,分别添加人参多糖[0.033 g/(只·d),T1组]、白术多糖[0.033 g/(只·d),T2组]、黄芪多糖[0.012 g/(只·d),T3组]、人参白术多糖复合物[0.016+0.01 g/(只·d),T4组]及生理盐水(0.7 mL/(只·d),T5组),试验结束前24 h小鼠一次性腹腔注射环磷酰胺200 mg/kg BW。T6~T10组为正常饲喂组,添加物、添加剂量分别与T1~T5组一致,饲喂30 d。试验结束后测定小鼠免疫器官指数、血清免疫指标以及血液中CD4+/CD8+值。结果显示:在免疫抑制模型中,与T5组相比,T4组可极显著提高小鼠平均日增重、脾脏指数和胸腺指数(P<0.05);正常饲喂情况下,与T10组相比,T9组中显著提高小鼠平均日增重(P<0.05),极显著提高小鼠胸腺指数(P<0.01)。在免疫抑制模型中,与T5组相比,T4组可极显著提高IL-2、TNF-α含量和CD4+/CD8+值(P<0.01),显著提高IgG含量;正常饲喂情况下,与T10组相比,T9组显著提高IL-2、TNF-α、IgM含量和CD4+/CD8+值(P<0.05)。综上表明人参白术多糖复合使用在提高动物机体免疫功能和颉颃免疫抑制剂方面效果要优于单独使用。

人参多糖干预创伤性骨关节炎模型大鼠前列腺素E_(2)/6-酮-前列腺素F_(1α)的表达

背景:目前已有研究发现植物人参提取物对骨关节炎有明显的改善作用,但是关于人参多糖提对骨关节炎的治疗作用尚未见报道。目的:探讨人参多糖干预创伤性骨关节炎模型大鼠前列腺素E_(2)/6-酮-前列腺素F_(1α)的表达变化。方法:选取60只雄性SD大鼠,随机分为健康组、模型组、人参多糖低、中、高剂量组、地塞米松组,除健康组外,其余大鼠均建立创伤性骨关节炎模型。造模成功后,健康组与模型组采用生理盐水0.2 mL腹腔注射,人参多糖低、中、高剂量组分别采用0.1,0.25,0.5μg/mL人参多糖0.2 mL腹腔注射,地塞米松组采用0.2 mg/kg地塞米松腹腔注射,均每3 d注射一次,连续干预4周。给药结束后采用ELISA法检测大鼠血清中前列腺素E_(2)、6-酮-前列腺素F_(1α)水平,Mankin’s评分法检测大鼠膝关节软骨功能,苏木精-伊红染色观察大鼠膝关节病理形态,免疫印迹与PCR分别检测关节软骨组织中肿瘤坏死因子α、白细胞介素1β、白细胞介素10的表达。结果与结论:①与模型组比较,人参多糖中剂量组、地塞米松组大鼠血清前列腺素E_(2)降低,6-酮-前列腺素F_(1α)升高(P<0.05);与人参多糖中剂量组、地塞米松组比较,人参多糖高剂量组大鼠上述指标显著改善(P<0.05);人参多糖中剂量组及地塞米松组无差异(P>0.05);②与模型组比较,人参多糖中剂量组、地塞米松组大鼠Mankin’s评分降低(P<0.05);与人参多糖中剂量组、地塞米松组比较,人参多糖高剂量组Mankin’s评分显著降低(P<0.05);人参多糖中剂量组及地塞米松组无差异(P>0.05);③模型组与人参多糖低剂量组大鼠软骨组织层明显变薄,深达骨质层的裂隙及软骨细胞大量丢失,潮线严重断裂、模糊,滑膜层胶原纤维增多、增粗,可见大量软骨细胞被破坏,排列不规则;人参多糖中剂量组、地塞米松组较模型组改善;人参多糖高剂量组较人参多糖中剂量组改善;④与模型组比较,人参多糖中剂量组、地塞米松组大鼠关节软骨组织中肿瘤坏死因子、白细胞介素1β表达降低,白细胞介素10表达升高(P<0.05);与人参多糖中剂量组、地塞米松组比较,人参多糖高剂量组大鼠骨关节中上述指标显著改善(P<0.05);人参多糖中剂量组及地塞米松组无差异(P>0.05);⑤提示人参多糖可改善创伤性骨关节炎大鼠炎性水平及病理形态,降低Mankin’s评分,其中人参多糖高剂量组效果最好,其作用机制可能与调控前列腺素E_(2)/6-酮-前列腺素F_(1α)表达水平有关。

西洋参花多糖闪式提取工艺优化及抗氧化活性研究

目的:研究西洋参花多糖闪式提取的最佳工艺条件及抗氧化活性。方法:以西洋参花为原料,考察提取电压、液料比、提取时间对西洋参花多糖得率的影响,采用响应面法优化西洋参花多糖闪式提取工艺。通过测定西洋参花多糖对DPPH自由基和羟基自由基的清除作用及总还原力,考察西洋参花多糖的抗氧化活性。结果:通过实验得到西洋参花多糖的最佳提取工艺条件为:提取电压:130 V,液料比:30:1 mL/g,提取时间:100 s。在此条件下,西洋参花多糖得率为11.12%±0.23%,与模型预测值相当;西洋参花多糖体外抗氧化显示其对DPPH自由基和羟基自由基清除率的IC_(50)值分别为1.34、1.42 mg/mL,且具有一定还原力。表明西洋参花具有较强的抗氧化活性,且随着多糖浓度的增加,抗氧化活性不断增强。结论:本实验得到的提取工艺条件具有可行性,可用于西洋参花多糖的提取。西洋参花多糖具有较好的抗氧化活性。本研究可为西洋参花的开发利用提供理论依据。

人参果多糖的分级分离纯化及组成成分

以人参果为原料,采用水提醇沉法提取人参果水溶性总多糖(the total water-soluble polysaccharide of ginseng fruit,WPGF),并以超滤-纤维素柱层析-凝胶柱层析三级分离模式,对人参果水溶性总多糖WPGF进行全面系统的分级分离纯化,得到人参果多糖一级级分(the high-polymer polysaccharide of ginseng fruit,WPGFH)1个、二级级分4个(WPGFH-0、WPGFH-0.04、WPGFH-0.08、WPGFH-0.12)、三级级分1个(WPGFH-0.08-1)。采用1-苯基-3-甲基-5-吡唑啉酮柱前衍生化高效液相色谱法(high performance liquid chromatography for pre-column derivatization of 1-phenyl-3-methyl-5-pyrazolone,PMPA-HPLC)和高效凝胶渗透色谱法(high performance gel permeation chromatography,HPGPC)初步分析人参果多糖4个二级级分和1个三级级分的单糖组成、分子质量均一性、分子质量范围,结果表明5个级分均主要含有葡萄糖、半乳糖、阿拉伯糖和鼠李糖及少量甘露糖,WPGFH-0和WPGFH-0.04不含糖醛酸,而其他级分含半乳糖醛酸和葡萄糖醛酸。结合红外光谱分析(Fourier transform infrared spectroscopic investigation,IR)对WPGFH-0.08-1级分进行了结构分析,初步推断其为阿拉伯半乳聚糖型果胶。为人参果多糖的研发提供参考及试验基础。

人参多糖辅助GP化疗方案治疗非小细胞肺癌的有效性和安全性研究

目的探讨人参多糖注射液辅助GP化疗方案治疗非小细胞肺癌的疗效。方法选择2011年6月~2013年6月收治的非小细胞肺癌患者130例,随机分为2组,每组65例。对照组给予吉西他滨和顺铂（GP方案）进行治疗,观察组在GP方案的基础上加用人参多糖注射液。连续治疗3个月后,对2组患者的治疗效果、T淋巴细胞亚群、不良反应、生存质量评分进行比较,随访1年后对2组患者的生存率进行比较。结果观察组的总有效率为58.5%,显著优于对照组（41.5%）,差异具有统计学意义（χ^2=3.72,P〈0.05）;观察组的CD3、CD4和CD4/CD8均显著高于对照组,差异具有统计学意义（t=5.735、7.885、7.326,均P〈0.05）,而CD8显著低于对照组,差异具有统计学意义（t=2.442,P〈0.05）;治疗过程中2组患者的主要不良反应为骨髓抑制（表现为白细胞下降、血小板下降和贫血等症状）和胃肠道反应;治疗后观察组的生存质量评分为（71.5±12.9）,显著高于对照组（64.2±12.2）,差异具有统计学意义（t=3.315,P〈0.05）;观察组的1年生存率为63.1%,对照组为52.3%,无显著差异。结论在GP化疗方案的基础上加用人参多糖注射液治疗非小细胞肺癌临床效果显著,免疫力显著增强,副作用风险降低,生存质量好。

参玫五味粉中总皂苷与多糖的提取及其体外活性研究

试验旨在探究参玫五味粉中总皂苷与多糖联合提取的最佳工艺条件及其体外活性。试验以参玫五味粉总皂苷与多糖的提取质量分数为考察指标,通过对提取溶剂浓度和浸泡时间的优化确定最佳提取工艺,测定参玫五味粉总皂苷提取物与多糖提取物对羟基自由基的清除率及对亚硝酸盐的抑制率,探索其体外抗氧化和抗肿瘤活性。结果显示:在最佳提取工艺条件下,联合提取4根层析柱中总皂苷的提取率依次为92.65%、98.99%、102.68%、104.31%,出膏率为10.00%,干浸膏中总皂苷含量为34.99 mg/g;多糖的提取率依次为93.57%、98.20%、100.77%、101.27%,出膏率为12.55%,干浸膏中多糖含量为19.20 mg/g。参玫五味粉总皂苷与多糖提取物清除羟基自由基的IC_(50) 值均大于对照VC,表明两者具有体外抗氧化活性,且具有一定的亚硝酸盐抑制作用。研究表明,柱层析联合提取法同时提取参玫五味粉总皂苷与多糖,提取率高且操作简便易行,可为畜牧业饲料添加剂的发展提供参考价值。

人参多糖辅助GP化疗方案治疗非小细胞肺癌的疗效观察

目的探讨人参多糖辅助GP方案治疗非小细胞肺癌(NSCLC)的临床疗效。方法选择2011年1月-2012年6月于我院收治的NSCLC患者80例,根据收治顺序分为观察组和对照组,每组40例。对照组给予GP化疗方案,即吉西他滨+顺铂;观察组在对照组的基础上加用人参多糖注射液,对两组患者的疗效、毒副反应、生存率等进行比较。结果观察组的总有效率显著优于对照组,差异具有统计学意义(x2=4.511,P<0.05);观察组不良反应中白细胞下降、血小板下降、贫血、胃肠道损伤和脱发的发生率均显著低于对照组,差异具有统计学意义(x2=14.25,12.53,4.604,7.031,4.945,P<0.05);观察组的1年生存率与对照组相比差异无统计学意义(P>0.05)。结论人参多糖辅助GP化疗方案治疗NSCLC疗效显著,毒副作用少,值得临床推广。

西洋参花多糖的提取工艺优化及其含量测定

目的探究西洋参花多糖最佳水提工艺,并建立西洋参花多糖含量的检测方法。方法利用水提法提取西洋参花多糖,通过单因素试验优化西洋参花多糖提取温度,正交试验以西洋参花多糖提取率为指标优化工艺参数。采用苯酚-硫酸法测定西洋参花多糖含量,并进行方法学研究。结果最佳提取工艺为95℃提取2.5 h,提取3次,料液比为1∶20(倍数),西洋参花多糖提取率为7.01%,人参花多糖提取率为6.54%,西洋参花多糖含量高于人参花。方法学试验显示,D-无水葡萄糖在65~325μg范围内与吸光度呈现较好的线性关系,平均加样回收率可达99.07%。结论该工艺有较高的提取效率,检测方法稳定可行,可为进一步分析西洋参花多糖组分及生理学评价提供试验依据。

不同光质激光对人参生长的影响

近年来,随着半导体激光器技术的不断进步,在农业领域应用的激光技术备受关注。激光光源具有单色性强、电光转换效率高、使用成本低等优势,为提高农作物质量、增加产量提供了新的技术支持。为了研究不同光质激光对人参生长、生理生化指标以及多糖、皂苷积累等方面的影响,选取二年生人参载子进行试验。从展叶期到叶片枯萎前的每日上午7:00—11:00,下午14:00—18:00分别利用全波长LED灯、红色激光灯(R3)、蓝色激光灯(B3)、混合激光灯(R2B1、R3B2)(光量子通量密度分别为4.48μmol/(m^(2)·s),4.61μmol/(m^(2)·s))补光,针对不同光质补光的影响,本研究设立了不补光作为空白对照组,研究发现,人参的净光合作用能力、叶片的气孔导度等都有所改善。特别是LED处理组的净光合速率表现最为突出,而蓝光处理组则在蒸腾速率和胞间CO_(2)浓度方面取得了最显著的效果提升。R3B2处理组气孔导度最大。就生长情况而言,人参在红蓝混合光处理下表现最佳,相比于对照组,蓝光能够显著提高植株的高度、增加叶片的长度和宽度,LED补光组根重、叶重均高于其他处理;从营养物质积累方面来看,蓝光处理下多糖含量最高,红光处理下皂苷含量积累普遍高于蓝光处理组,说明红光促进人参皂苷含量的积累,R2B1处理组Rg1、Rb1皂苷含量最高,远高于其他处理,R3B2处理时Re皂苷含量与红光处理组结果相当,高于其他4个处理。在未来的研究中,我们将选取最适宜人参生长及营养物质积累的激光补光条件,为缩短人参生长周期、提质增效而提供可行性方法。

星点设计优化人参叶多糖的超声辅助提取

为建立一种分离人参叶多糖的绿色高效工艺,本试验利用超声辅助水提取人参叶多糖,采用响应面的星点设计(CCD)优化多糖提取产率,利用乙醇沉淀分离出多糖提取物,并检测分析产物中的多糖与半乳糖醛酸含量。结果表明:CCD优化的工艺条件为液料比21.28 mL/g,超声时间58.33 min,超声功率456.67 W,此条件下人参叶多糖提取产率的预测值为2.07%。;在液料比21 mL/g,超声时间60 min,超声功率450 W即修订后的优化参数下,人参叶多糖提取产率为1.95%;提取产物中多糖含量为20.03%,半乳糖醛酸含量为13.79%。该工艺高效节能,可作为人参叶多糖高效分离的方法。

日粮中添加党参多糖对断奶仔猪生长性能、血清生化和免疫指标的影响

该试验将日龄、体重(9.00±0.25 kg)接近的健康的杜×长×大三元杂交断奶仔猪60头,随机分成4组,每组3个重复,每个重复5头仔猪,对照组饲喂基础日粮,试验Ⅰ组到Ⅲ组分别饲喂含有0.3%、 0.6%、 0.9%党参多糖的基础日粮。结果显示:与对照组相比,试验Ⅰ、 Ⅱ、 Ⅲ组断奶仔猪末重、平均日增重和平均日采食量均显著提高(P<0.05);试验Ⅰ、 Ⅱ、 Ⅲ组断奶仔猪的料重比均显著降低(P<0.05)。与对照组相比,试验Ⅰ、 Ⅱ、 Ⅲ组断奶仔猪血清中的总蛋白、球蛋白和碱性磷酸酶的含量均显著提高(P<0.05);试验Ⅰ、 Ⅱ、 Ⅲ组断奶仔猪血清中的谷草转氨酶、谷丙转氨酶、尿素氮和肌酐的含量有所提升,但差异不显著(P>0.05)。与对照组相比,试验Ⅰ、 Ⅱ、 Ⅲ组断奶仔猪血清中的免疫球蛋白G含量均显著提高(P<0.05)。可见,党参多糖可改善断奶仔猪免疫能力和生长性能,在日粮中添加0.9%党参多糖效果较好。

星点设计-响应面法优化人参硒多糖的制备工艺

目的优化硒化人参多糖的制备工艺。方法采用水提纯沉法提取多糖,用硝酸-亚硒酸钠法对多糖进行硒化,在单因素实验的基础上对人参多糖的硒化工艺进行响应面分析,采用紫外和红外光谱进行表征。结果当温度为71℃、时间为6.52h、Na_(2)SeO_(3)质量为524mg时,硒化人参多糖中的硒含量可达4.217mg·g^(-1),红外谱图上可观察到硒的特征峰。结论所得工艺条件稳定可靠,可为人参硒化多糖的工艺条件提供理论依据与实验参考。

人参多糖分散片制备工艺及其免疫活性研究

旨在确定人参多糖分散片的处方、制备工艺并检测免疫活性。采用单因素实验法对崩解剂、填充剂和润滑剂进行考察,以性状、崩解时限、硬度为考察指标,优化人参多糖分散片的处方;通过对小鼠脏器指数、迟发性变态反应、吞噬指数和自然杀伤细胞活性的测定考察人参多糖分散片的免疫活性。结果表明,人参多糖分散片的优选处方为31.25%人参粗多糖作主药,38.67%微晶纤维素与19.08%α-乳糖作复合填充剂,10%交联聚乙烯吡咯烷酮作崩解剂,1%硬脂酸镁作润滑剂,50%乙醇作润湿剂。免疫活性试验结果表明,人参多糖分散片可以显著提高小鼠的脏器指数、足跖肿胀程度、吞噬指数和自然杀伤细胞活性,具有良好的免疫增强活性。其中2.50 g/(kg·bw)人参多糖分散片的免疫活性最强,可作为潜在的保健食品或营养补充剂。