Three compounds were isolated from the tissue culture cells of Daphne giraldii cullus, their structures were identified as daphneolone （1）, S-（＋）-1-（4-hydroxy-3-methoxyphenyl）-3-hydroxy-5-phenyl-l-pentanone （2...Three compounds were isolated from the tissue culture cells of Daphne giraldii cullus, their structures were identified as daphneolone （1）, S-（＋）-1-（4-hydroxy-3-methoxyphenyl）-3-hydroxy-5-phenyl-l-pentanone （2）, S-（＋）-1-（4-methoxyphenyl）-3- hydroxy-5-phenyl-l-pentanone （3）, and among them, 2 was a new compound, 3 was a novel natural product.展开更多
A new compound, named daphnolin （1）, was isolated from the stem barks of Daphne giraldii Nitsche. Its structure was elucidated as 8-hydroxy-7-O-β-D-glucopyranosyl-5-（2-oxo-2H-1-benzopyran-7-hydroxy-8-yloxy）-2H-1-...A new compound, named daphnolin （1）, was isolated from the stem barks of Daphne giraldii Nitsche. Its structure was elucidated as 8-hydroxy-7-O-β-D-glucopyranosyl-5-（2-oxo-2H-1-benzopyran-7-hydroxy-8-yloxy）-2H-1-benzopyran-2-one by means of NMR and MS analysis.展开更多
A new coumarin glycoside was isolated from the ethanol extract of the barks of stem of Daphne giraldii Nitsche. Its structure was defined as daphnetin 8-O-β-D-glucopyranosyl-（1 → 6）-β-D-glucopyranoside on the bas...A new coumarin glycoside was isolated from the ethanol extract of the barks of stem of Daphne giraldii Nitsche. Its structure was defined as daphnetin 8-O-β-D-glucopyranosyl-（1 → 6）-β-D-glucopyranoside on the basis of spectral evidences.展开更多
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and orthogonal design were adopted to study the extraction technol...[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and orthogonal design were adopted to study the extraction technology for total phenols of leaves in Acanthopanax giraldii Harms. [Results]The optimum extraction conditions were determined as follows: extraction solvent( 70% methanol),solid-liquid ratio( 1∶ 35),and reflux extraction time( 60 min). Under these conditions,the yield of crude extract from total phenols of leaves in Acanthopanax giraldii Harms was 38. 67 mg/g.[Conclusions] The technology was simple,and of low cost and high extraction rate,suitable for the extraction of total phenols of leaves in Acanthopanax giraldii Harms.展开更多
In a search for structurally interesting substances from traditional Chinese medicines, eight compounds were isolated from an ethanolic extract of the stem bark of Daphne giraldii Nitsche. On the basis of one- and two...In a search for structurally interesting substances from traditional Chinese medicines, eight compounds were isolated from an ethanolic extract of the stem bark of Daphne giraldii Nitsche. On the basis of one- and two-dimensional nuclear magnetic resonance and mass spectrometry data, and chemical methods, their structures were determined to be 2H-1-benzopyran-2-one-8-hydroxy-7-O-β-D-glucopyranosyl-5-（2-oxo- 2H-1-benzopyran-7-hydroxy-8-yloxy） （compound 1）, 1-pentanone, 1-（4-hydroxyphenyl）-5-phenyl （compound 2）, octadecyl caffeate （compound 3）, （＋）syringaresinol-4,4＇-diglycoside （compound 4）, daphnetin-8-O-β-D- glucopyranoside （compound 5）, p-hydroxybenzoic acid （compound 6）, 7,7＇-dihydroxy-[6, 8＇-bi-2H-benzopyran]- 2, 2＇-dione （compound 7）, and daphnorin （compound 8）, respectively. Of the compounds isolated, compounds 1 and 2, which we named daphnolin and daphnolon, respectively, were new, and the others were obtained from this plant for the first time.展开更多
Objective:To study the anti-inflammatory mechanisms of total glycosides of Acanthopanax Giraldii (TGA).Methods:The changes of prostaglandin E_2(PGE_2),tumor necrosis factor(TNF-α),nitric oxide(NO), and expressions of...Objective:To study the anti-inflammatory mechanisms of total glycosides of Acanthopanax Giraldii (TGA).Methods:The changes of prostaglandin E_2(PGE_2),tumor necrosis factor(TNF-α),nitric oxide(NO), and expressions of COX-1 mRNA and COX-2 mRNA in BALB/c mouse macrophages were observed by the radioimmunoassay,ELISA and nitric acid reduction and RT-PCR in the presence or absence of TGA.Results: (1) TGA could significantly decrease the production of PGE_2 and NO in mouse peritoneal macrophages.The inhibitory ra...展开更多
A phytochemical investigation on the aerial parts of Artemisia giraldii var. longipedunculata led to the isolation of 10 known sesquiterpenes, including 12-hydroxy-α-cyperone（1）, 1β,6α-dihydroxyeudesma-4（15）-en...A phytochemical investigation on the aerial parts of Artemisia giraldii var. longipedunculata led to the isolation of 10 known sesquiterpenes, including 12-hydroxy-α-cyperone（1）, 1β,6α-dihydroxyeudesma-4（15）-ene（2）, carainterol A（3）, oplodiol（4）, douglanin（5）, 1α-hydroxyisodauc-4-en-15-al（6）, 3α,6α-dihydroxybisabola-4,10-diene（7）, 4α,5β-dihydroxybisabola-2,10-diene（8）, opposit-4（15）-ene-1β,7-diol（9）, and saniculamoid D（10）, respectively. Their structures were elucidated by analysis of the MS and NMR spectroscopic data and comparison with the literature. All the isolates were obtained from A. giraldii var. longipedunculata for the first time, and five of them were obtained from the genus Artemisia for the first time. Additionally, the 13 C NMR data of 1 were fully assigned based on the 2 D NMR data for the first time.展开更多
文摘Three compounds were isolated from the tissue culture cells of Daphne giraldii cullus, their structures were identified as daphneolone （1）, S-（＋）-1-（4-hydroxy-3-methoxyphenyl）-3-hydroxy-5-phenyl-l-pentanone （2）, S-（＋）-1-（4-methoxyphenyl）-3- hydroxy-5-phenyl-l-pentanone （3）, and among them, 2 was a new compound, 3 was a novel natural product.
文摘A new compound, named daphnolin （1）, was isolated from the stem barks of Daphne giraldii Nitsche. Its structure was elucidated as 8-hydroxy-7-O-β-D-glucopyranosyl-5-（2-oxo-2H-1-benzopyran-7-hydroxy-8-yloxy）-2H-1-benzopyran-2-one by means of NMR and MS analysis.
文摘A new coumarin glycoside was isolated from the ethanol extract of the barks of stem of Daphne giraldii Nitsche. Its structure was defined as daphnetin 8-O-β-D-glucopyranosyl-（1 → 6）-β-D-glucopyranoside on the basis of spectral evidences.
基金Supported by Sichuan Science and Technology Support Program(2015SZ-0034)Innovative Research Project for 2016 Graduate in Southwest University for Nationalities(CX2016SZ013)
文摘[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and orthogonal design were adopted to study the extraction technology for total phenols of leaves in Acanthopanax giraldii Harms. [Results]The optimum extraction conditions were determined as follows: extraction solvent( 70% methanol),solid-liquid ratio( 1∶ 35),and reflux extraction time( 60 min). Under these conditions,the yield of crude extract from total phenols of leaves in Acanthopanax giraldii Harms was 38. 67 mg/g.[Conclusions] The technology was simple,and of low cost and high extraction rate,suitable for the extraction of total phenols of leaves in Acanthopanax giraldii Harms.
文摘In a search for structurally interesting substances from traditional Chinese medicines, eight compounds were isolated from an ethanolic extract of the stem bark of Daphne giraldii Nitsche. On the basis of one- and two-dimensional nuclear magnetic resonance and mass spectrometry data, and chemical methods, their structures were determined to be 2H-1-benzopyran-2-one-8-hydroxy-7-O-β-D-glucopyranosyl-5-（2-oxo- 2H-1-benzopyran-7-hydroxy-8-yloxy） （compound 1）, 1-pentanone, 1-（4-hydroxyphenyl）-5-phenyl （compound 2）, octadecyl caffeate （compound 3）, （＋）syringaresinol-4,4＇-diglycoside （compound 4）, daphnetin-8-O-β-D- glucopyranoside （compound 5）, p-hydroxybenzoic acid （compound 6）, 7,7＇-dihydroxy-[6, 8＇-bi-2H-benzopyran]- 2, 2＇-dione （compound 7）, and daphnorin （compound 8）, respectively. Of the compounds isolated, compounds 1 and 2, which we named daphnolin and daphnolon, respectively, were new, and the others were obtained from this plant for the first time.
文摘Objective:To study the anti-inflammatory mechanisms of total glycosides of Acanthopanax Giraldii (TGA).Methods:The changes of prostaglandin E_2(PGE_2),tumor necrosis factor(TNF-α),nitric oxide(NO), and expressions of COX-1 mRNA and COX-2 mRNA in BALB/c mouse macrophages were observed by the radioimmunoassay,ELISA and nitric acid reduction and RT-PCR in the presence or absence of TGA.Results: (1) TGA could significantly decrease the production of PGE_2 and NO in mouse peritoneal macrophages.The inhibitory ra...
基金National Natural Science Foundation of China(NSFC,Grant No.81373294)National Key Technology R&D Program"New Drug Innovation"of China(Grant No.2018ZX09711001-008-003)
文摘A phytochemical investigation on the aerial parts of Artemisia giraldii var. longipedunculata led to the isolation of 10 known sesquiterpenes, including 12-hydroxy-α-cyperone（1）, 1β,6α-dihydroxyeudesma-4（15）-ene（2）, carainterol A（3）, oplodiol（4）, douglanin（5）, 1α-hydroxyisodauc-4-en-15-al（6）, 3α,6α-dihydroxybisabola-4,10-diene（7）, 4α,5β-dihydroxybisabola-2,10-diene（8）, opposit-4（15）-ene-1β,7-diol（9）, and saniculamoid D（10）, respectively. Their structures were elucidated by analysis of the MS and NMR spectroscopic data and comparison with the literature. All the isolates were obtained from A. giraldii var. longipedunculata for the first time, and five of them were obtained from the genus Artemisia for the first time. Additionally, the 13 C NMR data of 1 were fully assigned based on the 2 D NMR data for the first time.
