Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some ...Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer(LC). This list includes EFEMP1, EPHA5, HOXA5, HOXA9, LHX1, MYF6, NID2, OTX1, PAX9, RARB, RASSF1 A, RXRG, SIX6, SKOR1 and TERT genes. The results of DNA samples from 40 cancer and 25 normal lung tissues showed a good diagnostic potential of selected RCGY sites in regulatory regions of MYF6, SIX6, RXRG, LHX1, RASSF1 A and TERT genes with relatively high sensitivity(80.0 %) and specificity(88.0 %) of LC detection in tumor DNA.展开更多
The effect of annealing on vertically aligned TiO2 NWs deposited by glancing angle deposition（GLAD）method on Si substrate using pressed and sintered TiO2 pellets as source material is studied.The FE-SEM images revea...The effect of annealing on vertically aligned TiO2 NWs deposited by glancing angle deposition（GLAD）method on Si substrate using pressed and sintered TiO2 pellets as source material is studied.The FE-SEM images reveal the retention of vertically aligned NWs on Si substrate after annealing process.The EDS analysis of TiO2NWs sample annealed at 600 ℃ in air for 1 h shows the higher weight percentage ratio of ～2.6（i.e.,72.27%oxygen and 27.73%titanium）.The XRD pattern reveals that the polycrystalline nature of anatase TiO2 dominates the annealed NWs sample.The electrical characteristics of Al/TiO2-NWs/TiO2-TF/p-Si（NW device） and Al/TiO2-TF/p-Si（TF device） based on annealed samples are compared.It is riveting to observe a lower leakage current of ～1.32 × 10^-7 A/cm^2 at ＋1 V with interface trap density of-6.71 × 10^11eV^-1cm^-2 in NW device compared to ～2.23 × 10^-2 A/cm^2 in TF device.The dominant leakage mechanism is investigated to be generally Schottky emission;however Poole-Frenkel emission also takes place during high reverse bias beyond 4 V for NWs and 3 V for TF device.展开更多
In order to verify the properties of the light propagating through a gradient-index （GRIN） fibre probe for optical coherence tomography （OCT）, numerical simulation using the optical software GLAD is carried out. F...In order to verify the properties of the light propagating through a gradient-index （GRIN） fibre probe for optical coherence tomography （OCT）, numerical simulation using the optical software GLAD is carried out. Firstly, the model of the GRIN fibre probe is presented, which is consisted of a single mode fibre （SMF）, a no-core fibre （NCF）, a GRIN fibre lens and an air path. Then, the software GLAD is adopted to numerically investigate how the lengths of the NCF and the GRIN fibre lens influence the performance of the Gaussian beam focusing through the GRIN fibre probe. The simulation results are well consistent with the experimental ones, showing that the GLAD based numerical simulation technique is an intuitive and effective tool for the verification of the properties of the light propagation. In this paper, we find that on the conditions of a constant GRIN fibre lens length of 0.1 mm and an NCF length of 0.36 mm, the working distance of the probe will be 0.75 mm and the focus spot size is 32 μm.展开更多
基金supported by the Skolkovo Foundation(Under agreement NO.G102/16 06.12.2016г.)
文摘Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer(LC). This list includes EFEMP1, EPHA5, HOXA5, HOXA9, LHX1, MYF6, NID2, OTX1, PAX9, RARB, RASSF1 A, RXRG, SIX6, SKOR1 and TERT genes. The results of DNA samples from 40 cancer and 25 normal lung tissues showed a good diagnostic potential of selected RCGY sites in regulatory regions of MYF6, SIX6, RXRG, LHX1, RASSF1 A and TERT genes with relatively high sensitivity(80.0 %) and specificity(88.0 %) of LC detection in tumor DNA.
文摘The effect of annealing on vertically aligned TiO2 NWs deposited by glancing angle deposition（GLAD）method on Si substrate using pressed and sintered TiO2 pellets as source material is studied.The FE-SEM images reveal the retention of vertically aligned NWs on Si substrate after annealing process.The EDS analysis of TiO2NWs sample annealed at 600 ℃ in air for 1 h shows the higher weight percentage ratio of ～2.6（i.e.,72.27%oxygen and 27.73%titanium）.The XRD pattern reveals that the polycrystalline nature of anatase TiO2 dominates the annealed NWs sample.The electrical characteristics of Al/TiO2-NWs/TiO2-TF/p-Si（NW device） and Al/TiO2-TF/p-Si（TF device） based on annealed samples are compared.It is riveting to observe a lower leakage current of ～1.32 × 10^-7 A/cm^2 at ＋1 V with interface trap density of-6.71 × 10^11eV^-1cm^-2 in NW device compared to ～2.23 × 10^-2 A/cm^2 in TF device.The dominant leakage mechanism is investigated to be generally Schottky emission;however Poole-Frenkel emission also takes place during high reverse bias beyond 4 V for NWs and 3 V for TF device.
基金supported by the International Cooperation Project of Shanghai Science and Technology Committee,China(Grant No.09530708700)
文摘In order to verify the properties of the light propagating through a gradient-index （GRIN） fibre probe for optical coherence tomography （OCT）, numerical simulation using the optical software GLAD is carried out. Firstly, the model of the GRIN fibre probe is presented, which is consisted of a single mode fibre （SMF）, a no-core fibre （NCF）, a GRIN fibre lens and an air path. Then, the software GLAD is adopted to numerically investigate how the lengths of the NCF and the GRIN fibre lens influence the performance of the Gaussian beam focusing through the GRIN fibre probe. The simulation results are well consistent with the experimental ones, showing that the GLAD based numerical simulation technique is an intuitive and effective tool for the verification of the properties of the light propagation. In this paper, we find that on the conditions of a constant GRIN fibre lens length of 0.1 mm and an NCF length of 0.36 mm, the working distance of the probe will be 0.75 mm and the focus spot size is 32 μm.
