用快中子脉冲堆(China fast burst reactor II,CFBR-II)对唐菖蒲“超级”球茎进行不同剂量的照射处理,研究快中子对唐菖蒲球茎表面的刻蚀情况及对蛋白质表达的阻遏作用。扫描电镜观察发现球茎受损伤情况随照射剂量和照射角度的不同而变...用快中子脉冲堆(China fast burst reactor II,CFBR-II)对唐菖蒲“超级”球茎进行不同剂量的照射处理,研究快中子对唐菖蒲球茎表面的刻蚀情况及对蛋白质表达的阻遏作用。扫描电镜观察发现球茎受损伤情况随照射剂量和照射角度的不同而变化,受垂直方向照射的球茎表面刻蚀程度最严重,且刻蚀程度随着剂量增大而增大。通过田间栽培试验和SDS-聚丙烯酰胺凝胶电泳(SDS-polyacrylamide gel electrophoresis,SDS-PAGE)分析发现,随照射剂量的增大,植株生长发育受到明显的抑制,蛋白表达数量显著减少。由此表明,快中子照射对植株能产生明显的影响。展开更多
Either bacterial attachment or cellulose fibrillar elaboration was hardly observedduring the cocultivation of the cultured suspension cells of Oryza sativa Indica with thestrain C58C1 Rif^r of Agrobacterium tumefacien...Either bacterial attachment or cellulose fibrillar elaboration was hardly observedduring the cocultivation of the cultured suspension cells of Oryza sativa Indica with thestrain C58C1 Rif^r of Agrobacterium tumefaciens that was not specially pretreated. Onthe other hand, quite a lot of Agrobacterium cells were found to adhere to the surface ofcultured rice cells and a number of cellulose fibrils were produced around the specifiedbacteria when phenolics-pretreated bacteria were cocultivated with rice suspension cellsin common culture media, especially in complex culture solutions. The complex culturesolution was the bacterium-free filtrate of hormone-containing MS medium which hadbeen utilized to incubate carrot cells and the newly wounded hypocotyl segments fromtomato and Agrobacterium cells. Detecting experiments demonstrated that both NPT Ⅱ andNOS genes, located on the T-DNA segment of chimaeric plasmid pGV3850 :: 1103neo, weretransferred and expressed in the cultured cells of O. sativa Indica in the optimum trans-formation condition of the present experiment. Integration of the foreign genes into riceDNA was confirmed by Southern blot hybridization.展开更多
文摘用快中子脉冲堆(China fast burst reactor II,CFBR-II)对唐菖蒲“超级”球茎进行不同剂量的照射处理,研究快中子对唐菖蒲球茎表面的刻蚀情况及对蛋白质表达的阻遏作用。扫描电镜观察发现球茎受损伤情况随照射剂量和照射角度的不同而变化,受垂直方向照射的球茎表面刻蚀程度最严重,且刻蚀程度随着剂量增大而增大。通过田间栽培试验和SDS-聚丙烯酰胺凝胶电泳(SDS-polyacrylamide gel electrophoresis,SDS-PAGE)分析发现,随照射剂量的增大,植株生长发育受到明显的抑制,蛋白表达数量显著减少。由此表明,快中子照射对植株能产生明显的影响。
文摘Either bacterial attachment or cellulose fibrillar elaboration was hardly observedduring the cocultivation of the cultured suspension cells of Oryza sativa Indica with thestrain C58C1 Rif^r of Agrobacterium tumefaciens that was not specially pretreated. Onthe other hand, quite a lot of Agrobacterium cells were found to adhere to the surface ofcultured rice cells and a number of cellulose fibrils were produced around the specifiedbacteria when phenolics-pretreated bacteria were cocultivated with rice suspension cellsin common culture media, especially in complex culture solutions. The complex culturesolution was the bacterium-free filtrate of hormone-containing MS medium which hadbeen utilized to incubate carrot cells and the newly wounded hypocotyl segments fromtomato and Agrobacterium cells. Detecting experiments demonstrated that both NPT Ⅱ andNOS genes, located on the T-DNA segment of chimaeric plasmid pGV3850 :: 1103neo, weretransferred and expressed in the cultured cells of O. sativa Indica in the optimum trans-formation condition of the present experiment. Integration of the foreign genes into riceDNA was confirmed by Southern blot hybridization.
