Background:As one of the representative protein materials,protein nanocages(PNCs)are self-assembled supramolecular structures with multiple advantages,such as good monodispersity,biocompatibility,structural addressabi...Background:As one of the representative protein materials,protein nanocages(PNCs)are self-assembled supramolecular structures with multiple advantages,such as good monodispersity,biocompatibility,structural addressability,and facile production.Precise quantitative functionalization is essential to the construction of PNCs with designed purposes.Results:With three modifiable interfaces,the interior surface,outer surface,and interfaces between building blocks,PNCs can serve as an ideal platform for precise multi-functionalization studies and applications.This review summarizes the currently available methods for precise quantitative functionalization of PNCs and highlights the significance of precise quantitative control in fabricating PNC-based materials or devices.These methods can be categorized into three groups,genetic,chemical,and combined modification.Conclusion:This review would be constructive for those who work with biosynthetic PNCs in diverse fields.展开更多
基金the National Natural Science Foundation of China(Nos.31771103 and 31800650)the Chinese Academy of Sciences(CAS)Emergency Project of African Swine Fever(ASF)Research(No.KJZD-SW-L06)+1 种基金Department of Education of Hubei Province(No.Q20221103)Heath Commission of Hubei Province(No.WJ2021M214).
文摘Background:As one of the representative protein materials,protein nanocages(PNCs)are self-assembled supramolecular structures with multiple advantages,such as good monodispersity,biocompatibility,structural addressability,and facile production.Precise quantitative functionalization is essential to the construction of PNCs with designed purposes.Results:With three modifiable interfaces,the interior surface,outer surface,and interfaces between building blocks,PNCs can serve as an ideal platform for precise multi-functionalization studies and applications.This review summarizes the currently available methods for precise quantitative functionalization of PNCs and highlights the significance of precise quantitative control in fabricating PNC-based materials or devices.These methods can be categorized into three groups,genetic,chemical,and combined modification.Conclusion:This review would be constructive for those who work with biosynthetic PNCs in diverse fields.
