A newly established human tumor clonogenecity assay - the glass capillary tube culture technique (CAP assay) to described. With this method, we observed the in vitro anticancer efficiency of several antineoplastics on...A newly established human tumor clonogenecity assay - the glass capillary tube culture technique (CAP assay) to described. With this method, we observed the in vitro anticancer efficiency of several antineoplastics on human solid tumors and acute non-lymphatic leukemia (ANLL) specimen. Comparing with the conventional two-layer agar culture assay (2-LAC assay). The CAP-assay had a higher culture success rate, higher plating efficiency (PE) and shorter experimental period. The preliminary data suggesting and that the CAP assay has the potential to replace the conventional 2- LAC assay for in vitro drug- sensitivity testing and may be applied clinically.展开更多
Background At present,the highest spatial resolution of a fast neutron imaging detector,mainly determined by the range of secondary particles generated by fast neutrons,is about hundreds of microns.In view of the abov...Background At present,the highest spatial resolution of a fast neutron imaging detector,mainly determined by the range of secondary particles generated by fast neutrons,is about hundreds of microns.In view of the above inherent spatial resolution limitation,a capillary-based scintillation detector that can improve the spatial resolution of fast neutron imaging by recording and reconstructing the recoil proton track was developed.Purpose The purpose of this paper is to develop a detector for recognizing recoil proton events,reconstructing particle track and improving the position resolution with track reconstruction method to reconstruct the position of interaction.Methods The proposed detector consists of a 1000×1000 array of glass capillaries loaded with a high refractive index liquid scintillator.Each glass capillary was 10μm in diameter and 5 cm in length.The recoil protons generated by the incident neutrons move within the detector and produce scintillation light within each capillary that they traverse.The light emitted from the capillary array can be recorded by employing an intensified CCD camera.We used Geant4 to simulate the detector performance and CERN ROOT analysis framework to record physical information of recoil proton,including position,energy deposition in each capillary and track length.Based on Hough transform,a rapid,computerized and efficient proton track reconstruction procedure was developed.Conclusion The recoil proton events display a continuous extended structure.The track reconstruction algorithms can reconstruct individual track precisely,and when the counting rate was relatively low,the track reconstruction results were in good agreement with simulation data.Moreover,for intensive overlap conditions,this algorithm also reconstructs periphery tracks with high rate of accuracy.展开更多
文摘A newly established human tumor clonogenecity assay - the glass capillary tube culture technique (CAP assay) to described. With this method, we observed the in vitro anticancer efficiency of several antineoplastics on human solid tumors and acute non-lymphatic leukemia (ANLL) specimen. Comparing with the conventional two-layer agar culture assay (2-LAC assay). The CAP-assay had a higher culture success rate, higher plating efficiency (PE) and shorter experimental period. The preliminary data suggesting and that the CAP assay has the potential to replace the conventional 2- LAC assay for in vitro drug- sensitivity testing and may be applied clinically.
基金This work was supported by the National Nature Science Foundation of China(Contract No.11805066).
文摘Background At present,the highest spatial resolution of a fast neutron imaging detector,mainly determined by the range of secondary particles generated by fast neutrons,is about hundreds of microns.In view of the above inherent spatial resolution limitation,a capillary-based scintillation detector that can improve the spatial resolution of fast neutron imaging by recording and reconstructing the recoil proton track was developed.Purpose The purpose of this paper is to develop a detector for recognizing recoil proton events,reconstructing particle track and improving the position resolution with track reconstruction method to reconstruct the position of interaction.Methods The proposed detector consists of a 1000×1000 array of glass capillaries loaded with a high refractive index liquid scintillator.Each glass capillary was 10μm in diameter and 5 cm in length.The recoil protons generated by the incident neutrons move within the detector and produce scintillation light within each capillary that they traverse.The light emitted from the capillary array can be recorded by employing an intensified CCD camera.We used Geant4 to simulate the detector performance and CERN ROOT analysis framework to record physical information of recoil proton,including position,energy deposition in each capillary and track length.Based on Hough transform,a rapid,computerized and efficient proton track reconstruction procedure was developed.Conclusion The recoil proton events display a continuous extended structure.The track reconstruction algorithms can reconstruct individual track precisely,and when the counting rate was relatively low,the track reconstruction results were in good agreement with simulation data.Moreover,for intensive overlap conditions,this algorithm also reconstructs periphery tracks with high rate of accuracy.
