Optimization of Polysaccharide Extraction from Radix Glehniae Root Bark by Response Surface Methodology and Anti-immunosuppressive Activity Analysis

[ Objective ] This study aimed to establish and optimize the extraction technology of polysaccharides from Radix Glehniae root bark, and to explore the processing methods of Radix Glehniae in producing areas. [ Method ] Based on the single-factor experiment, with polysaccharide content and polysaccharide yield as indices, various factors affecting polysaccharide yield from Radix Glehniae root bark were investigated by central composite design-response surface method. Mo- reover, immune functions of cyclophosphamide-induced blood-deficient mice in various polysaccharide groups were compared to investigate the effects of root bark removal of Radix Glehniae on medicinal quality. [ Result] Ultrasonic extraction was the appropriate extraction technology of polysaccharides from Radix Glehniae root bark. The optimal ultrasonic extraction conditions were ultrasonic time 36 min, ultrasonic power 460 W, solid-liquid ratio 1： 10, ethanol concentration 80%, under which polysaccharide yield reached 16.08%. Polysaccharides extracted from Radix Glehniae root （ALP） and Radix Glehniae root bark （BLP） could effec- tively improve immune function of blood-deficient mice with no significant differences. [ Conclusion] Radix Glehniae could be used without removal of root bark. The optimized extraction process was stable and feasible, which laid a solid foundation for further comprehensive development and utilization of Radix Glehniae root bark.

A review of research on coumarins from Radix Glehniae

Radix Glehniae,with a wide range of chemical components of various pharmacological effects,is widely used in the prevention and treatment of various diseases in clinical practice.It has a broad prospect of development and application.Coumarin,as one of its major active components,also displays good biological activities for analgesia and sedation,antibacteria,antivirus,anti HIV,anti-inflammation,anti-tumor,anti mutation,tyrosinase inhibition,vasodilation and blood pressure reduction.The aim of the present review is to discuss the studies on coumarin components,content determination,pharmacological activities,processing methods and provide a guide for the further development and utilization of Radix Glehniae.