The influences of hydrophobic SiO_2 nanoparticles and the contents of glucose oxidase(GOD) upon the response current of enzyme electrode have been investigated by using the sol-gel method,in which polyvinylbutyryl(PVB...The influences of hydrophobic SiO_2 nanoparticles and the contents of glucose oxidase(GOD) upon the response current of enzyme electrode have been investigated by using the sol-gel method,in which polyvinylbutyryl(PVB)was used as a matrix.The influenoe of enzymatic activity was measured by electrochemical method.Experimental data demonstrated that hydrophobic SiO_2 particles can immobilize enzyme well,providing a good and simple method for preparing high quality GOD biosensor.The mechanism has been discussed.展开更多
In this paper, a novel biosensor was prepared by immobilizing glucose oxidase （GOx） on carbon nanotube-gold-titania nanocomposites （CNT/Au/TiO2） modified glassy carbon electrode （GCE）. SEM was initially used to ...In this paper, a novel biosensor was prepared by immobilizing glucose oxidase （GOx） on carbon nanotube-gold-titania nanocomposites （CNT/Au/TiO2） modified glassy carbon electrode （GCE）. SEM was initially used to investigate the surface morphology of CNT/Au/TiO2 nanocomposites modified GCE, indicating the formation of the nano-porous structure which could readily facilitate the attachment of GOx on the electrode surface. Cyclic voltammogram （CV） and electrochemical impedance spectrum （EIS） were further utilized to explore relevant electrochemical activity on CNT]Au/TiO2 nanocomposites modified GCE. The observations demonstrated that the immobilized GOx could efficiently execute its bioelectrocatalytic activity for the oxidation of glucose. The biosensor exhibited a wider linearity range from 0.1 mmol L-1 to 8 mmol L^-1 glucose with a detection limit of 0.077 mmol L^- 1.展开更多
A novel photoelectrochemical biosensor incorporating nanosized CdS semiconductor crystals with enzyme to enhance photochemical reaction has been investigated. CdS nanoparticles were synthesized by using dendrimer PAMA...A novel photoelectrochemical biosensor incorporating nanosized CdS semiconductor crystals with enzyme to enhance photochemical reaction has been investigated. CdS nanoparticles were synthesized by using dendrimer PAMAM as inner templates. The CdS nanoparticles and glucose oxidase （GOD） were immobilized on Pt electrode via layer-by-layer （LbL） technique to fabricate a biological-inorganic hybrid system. Under ultraviolet light, the photo-effect of the CdS nanoparticles showed enhancement of the biosensor to detect glucose. Pt nanoparticles were mixed into the Nation film to immobilize the CdS/enzyme composites and to improve the charge transfer of the hybrid. Experimental results demonstrate the desirable characteristics of this biosensing system, e,g. a sensitivity of 1.83 μA/（mM cm^2）, lower detection limit （1 μM）, and acceptable reproducibility and stability,展开更多
文摘The influences of hydrophobic SiO_2 nanoparticles and the contents of glucose oxidase(GOD) upon the response current of enzyme electrode have been investigated by using the sol-gel method,in which polyvinylbutyryl(PVB)was used as a matrix.The influenoe of enzymatic activity was measured by electrochemical method.Experimental data demonstrated that hydrophobic SiO_2 particles can immobilize enzyme well,providing a good and simple method for preparing high quality GOD biosensor.The mechanism has been discussed.
基金support from National Key Basic Research Program (No. 2010CB732404)National Natural Science Foundation of China (No. 21175020)+1 种基金Qinglan Project Sci-Tech Innovation Team of Jiangsu ProvinceSuzhou Science & Technology Major Project (No. ZXY2012028)
文摘In this paper, a novel biosensor was prepared by immobilizing glucose oxidase （GOx） on carbon nanotube-gold-titania nanocomposites （CNT/Au/TiO2） modified glassy carbon electrode （GCE）. SEM was initially used to investigate the surface morphology of CNT/Au/TiO2 nanocomposites modified GCE, indicating the formation of the nano-porous structure which could readily facilitate the attachment of GOx on the electrode surface. Cyclic voltammogram （CV） and electrochemical impedance spectrum （EIS） were further utilized to explore relevant electrochemical activity on CNT]Au/TiO2 nanocomposites modified GCE. The observations demonstrated that the immobilized GOx could efficiently execute its bioelectrocatalytic activity for the oxidation of glucose. The biosensor exhibited a wider linearity range from 0.1 mmol L-1 to 8 mmol L^-1 glucose with a detection limit of 0.077 mmol L^- 1.
基金support from the National Natural Science Foundation of China (Grant No.20676038)the Key Project of Science and Technology for Ministry of Education (Grant No. 107045)+1 种基金the Program of Shanghai Subject Chief Scientist (08XD1401500)the Shanghai Leading Academic Discipline Project (Project Number:B502)
文摘A novel photoelectrochemical biosensor incorporating nanosized CdS semiconductor crystals with enzyme to enhance photochemical reaction has been investigated. CdS nanoparticles were synthesized by using dendrimer PAMAM as inner templates. The CdS nanoparticles and glucose oxidase （GOD） were immobilized on Pt electrode via layer-by-layer （LbL） technique to fabricate a biological-inorganic hybrid system. Under ultraviolet light, the photo-effect of the CdS nanoparticles showed enhancement of the biosensor to detect glucose. Pt nanoparticles were mixed into the Nation film to immobilize the CdS/enzyme composites and to improve the charge transfer of the hybrid. Experimental results demonstrate the desirable characteristics of this biosensing system, e,g. a sensitivity of 1.83 μA/（mM cm^2）, lower detection limit （1 μM）, and acceptable reproducibility and stability,
