期刊文献+
共找到187篇文章
< 1 2 10 >
每页显示 20 50 100
Combined ATAC-seq,RNA-seq,and GWAS analysis reveals glycogen metabolism regulatory network in Jinjiang oyster(Crassostrea ariakensis)
1
作者 Biao Wu Xi Chen +12 位作者 Jie Hu Zhen-Yuan Wang Yan Wang Da-You Xu Hao-Bing Guo Chang-Wei Shao Li-Qing Zhou Xiu-Jun Sun Tao Yu Xiao-Mei Wang Yan-Xin Zheng Guang-Yi Fan Zhi-Hong Liu 《Zoological Research》 SCIE CSCD 2024年第1期201-214,共14页
Glycogen serves as the principal energy reserve for metabolic processes in aquatic shellfish and substantially contributes to the flavor and quality of oysters.The Jinjiang oyster(Crassostrea ariakensis)is an economic... Glycogen serves as the principal energy reserve for metabolic processes in aquatic shellfish and substantially contributes to the flavor and quality of oysters.The Jinjiang oyster(Crassostrea ariakensis)is an economically and ecologically important species in China.In the present study,RNA sequencing(RNA-seq)and assay for transposase-accessible chromatin using sequencing(ATAC-seq)were performed to investigate gene expression and chromatin accessibility variations in oysters with different glycogen contents.Analysis identified 9483 differentially expressed genes(DEGs)and 7215 genes with significantly differential chromatin accessibility(DCAGs)were obtained,with an overlap of 2600 genes between them.Notably,a significant proportion of these genes were enriched in pathways related to glycogen metabolism,including“Glycogen metabolic process”and“Starch and sucrose metabolism”.In addition,genome-wide association study(GWAS)identified 526 single nucleotide polymorphism(SNP)loci associated with glycogen content.These loci corresponded to 241 genes,63 of which were categorized as both DEGs and DCAGs.This study enriches basic research data and provides insights into the molecular mechanisms underlying the regulation of glycogen metabolism in C.ariakensis. 展开更多
关键词 Crassostrea ariakensis GLYCOGEN TRANSCRIPTOME ATAC GWAS
下载PDF
Dose-response effect of pre-exercise carbohydrates under muscle glycogen unavailability:Insights from McArdle disease
2
作者 Pedro L.Valenzuela Alfredo Santalla +8 位作者 Lidia B.Alejo Andrea Merlo Asuncion Bustos Laura Castellote-Belles Roser Ferrer-Costa Nicola A.Maffiuletti David Barranco-Gil Tomas Pinos Alejandro Lucia 《Journal of Sport and Health Science》 SCIE CAS CSCD 2024年第3期398-408,共11页
Background:This study aimed to determine the effect of different carbohydrate(CHO)doses on exercise capacity in patients with McArdle disease—the paradigm of“exercise intolerance”,characterized by complete muscle g... Background:This study aimed to determine the effect of different carbohydrate(CHO)doses on exercise capacity in patients with McArdle disease—the paradigm of“exercise intolerance”,characterized by complete muscle glycogen unavailability—and to determine whether higher exogenous glucose levels affect metabolic responses at the McArdle muscle cell(in vitro)level.Methods:Patients with McArdle disease(n=8)and healthy controls(n=9)underwent a 12-min submaximal cycling constant-load bout followed by a maximal ramp test 15 min after ingesting a non-caloric placebo.In a randomized,double-blinded,cross-over design,patients repeated the tests after consuming either 75 g or 150 g of CHO(glucose:fructose=2:1).Cardiorespiratory,biochemical,perceptual,and electromyographic(EMG)variables were assessed.Additionally,glucose uptake and lactate appearance were studied in vitro in wild-type and McArdle mouse myotubes cultured with increasing glucose concentrations(0.35,1.00,4.50,and 10.00 g/L).Results:Compared with controls,patients showed the“classical”second-wind phenomenon(after prior disproportionate tachycardia,myalgia,and excess electromyographic activity during submaximal exercise,all p<0.05)and an impaired endurance exercise capacity(-51%ventilatory threshold and55%peak power output,both p<0.001).Regardless of the CHO dose(p<0.05 for both doses compared with the placebo),CHO intake increased blood glucose and lactate levels,decreased fat oxidation rates,and attenuated the second wind in the patients.However,only the higher dose increased ventilatory threshold(+27%,p=0.010)and peak power output(+18%,p=0.007).In vitro analyses revealed no differences in lactate levels across glucose concentrations in wild-type myotubes,whereas a doseresponse effect was observed in McArdle myotubes.Conclusion:CHO intake exerts beneficial effects on exercise capacity in McArdle disease,a condition associated with total muscle glycogen unavailability.Some of these benefits are dose dependent. 展开更多
关键词 ENDURANCE Glycogen storage disease GLYCOGENOSIS NUTRITION SUPPLEMENT
下载PDF
Glycogen metabolism-mediated intercellular communication in the tumor microenvironment influences liver cancer prognosis
3
作者 YANG ZHANG NANNAN QIN +6 位作者 XIJUN WANG RUI LIANG QUAN LIU RUOYI GENG TIANXIAO JIANG YUNFEI LIU JINWEI LI 《Oncology Research》 SCIE 2024年第3期563-576,共14页
Glycogen metabolism plays a key role in the development of hepatoellular carcinoma(HCC),but the function of glycogen metabolism genes in the tumor microenvironment(TME)is still to be elucidated.Single cell RNA-seq dat... Glycogen metabolism plays a key role in the development of hepatoellular carcinoma(HCC),but the function of glycogen metabolism genes in the tumor microenvironment(TME)is still to be elucidated.Single cell RNA-seq data were obtained from ten HCC tumor samples totaling 64,545 cells and 65 glycogen metabolism genes were analyzed bya nonnegative matrix factorization(NMF).The prognosis and immune response of new glycogen TME cell dusters were predicted by using HCC and immunotherapy cohorts from public databases.HOC single cell analysis was divided into fibroblasts,NT T cells,macrophages,endothelial clls,and B cells,which were separately divided into new cell clusters by glycogen metabolism gene annotation.Pseudo temporal trajectory analysis demonstrated the temporal differentiation trajectory of different glycogen subtype cell dusters.Cellular communication analysis revealed extensive interactions between endothelial cells with glycogen metabolizing TME cell.related subtypes and diferent glycogen subtype cell clusters.SCENIC analysis of transcription factors upstream of TME cell clusters with different glycogen metabolism.In addition,TME cell dusters of glycogen metabolism were found to be enriched in expression in CAF subtypes,CD8 depleted,M1,and M2 types.Bulk seq analysis showed the prognostic signifcance of glycogen metabolism.mediated TME cell dusters in HCC,while a significant immune response was found in the immunotherapy cohort in patients treated with immune checkpoint blockade(ICB),especially for CAFs,T cells,and macrophages In summary,our study reveals for the first time that glycogen metabolism mediates intercellular communication in the hepatocellular carcinoma microenvironment while elucidating the anti-tumor mechanisms and immune prognostic responses of different subtypes of cell dusters. 展开更多
关键词 Glycogen metabolism Metabolic map Single cell Tumor microenvironment Liver cancer PROGNOSIS IMMUNOTHERAPY
下载PDF
电项针对全脑缺血VD模型大鼠PI3K/AKT/GSK-3β信号通路的影响 被引量:15
4
作者 陈晶 胡新颖 +1 位作者 刘勇 韩鹏 《世界中西医结合杂志》 2018年第2期200-203,288,共5页
目的研究电项针对全脑缺血血管性痴呆(vascular dementia,VD)模型大鼠磷脂酰肌醇-3-激酶/丝氨酸-苏氨酸蛋白激酶/糖原合成酶激酶-3β(Phosphatidylinositol-3 kinase/serine-threonine kinase/glycogen synthase kinase-3β,P13K/AKT/GS... 目的研究电项针对全脑缺血血管性痴呆(vascular dementia,VD)模型大鼠磷脂酰肌醇-3-激酶/丝氨酸-苏氨酸蛋白激酶/糖原合成酶激酶-3β(Phosphatidylinositol-3 kinase/serine-threonine kinase/glycogen synthase kinase-3β,P13K/AKT/GSK-3β)信号通路的影响。方法采用四血管阻断方法制备VD模型大鼠,电项针组取双侧风池穴、供血穴,电针30 min/次,1次/d,治疗14d。采用Y迷宫评价大鼠学习记忆能力;荧光定量PCR(RT-PCR)、Western blot法检测大鼠海马组织中磷酸化蛋白激酶B(phosphorylatedproteinkinaseB,p-AKT)、磷酸化糖原合成酶激酶-3β(Phosphorylated GSK-3β,P-GSK-3β)mRNA和p-AKT、p-GSK-3β蛋白的表达。结果与模型组比较,电项针组可显著提高VD大鼠Y迷宫学习与记忆正确次数(P<0.01)。与模型组比较,电项针组大鼠海马组织中p-AKT、p-GSK-3βmRNA和p-AKT、p-GSK-3β蛋白表达均有不同程度的升高(P<0.01)。结论电项针能够改善VD模型大鼠学习记忆能力,具体机制可能是激活PI3K/AKT/GSK-3β信号通路,发挥抗凋亡作用,起到对缺血海马神经元的保护作用。 展开更多
关键词 电项针 血管性痴呆 全脑缺血 磷脂酰肌醇-3-激酶/丝氨酸-苏氨酸蛋白激酶/糖原合成酶激酶-3β(Phosphatidylinositol-3 kinase/serine-threonine kinase/glycogen synthase kinase-3β P13K/AKT/GSK
下载PDF
脂多糖预处理导致的糖原合成酶激酶-3抑制对肝糖原的影响和机制 被引量:2
5
作者 陈小乐 龚建平 徐发良 《南方医科大学学报》 CAS CSCD 北大核心 2014年第2期201-205,共5页
目的探讨脂多糖预处理时糖原合成酶激酶3(glycogen synthase kinase-3,GSK-3)功能活性的变化及其对肝组织糖原代谢的影响和机制。方法雄性SD大鼠随机分为正常对照、脂多糖预处理和GSK-3抑制剂氯化锂预处理组,分别进行相应处理后再接受... 目的探讨脂多糖预处理时糖原合成酶激酶3(glycogen synthase kinase-3,GSK-3)功能活性的变化及其对肝组织糖原代谢的影响和机制。方法雄性SD大鼠随机分为正常对照、脂多糖预处理和GSK-3抑制剂氯化锂预处理组,分别进行相应处理后再接受大剂量脂多糖(10 mg/kg)攻击以建立脂多糖诱导的急性肝损伤模型;采用PAS染色法观察肝组织糖原聚集,用试剂盒法定量检测肝组织糖原含量,以Western Blot法半定量分析GSK-3的蛋白表达和抑制性磷酸化水平,采用考马斯亮兰比色法测定肝组织钙依赖蛋白酶的活性。结果尽管大剂量脂多糖攻击后各组动物肝组织糖原含量组间比较均无显著差异(P>0.05),但均较攻击前有显著降低(P<0.05),且脂多糖和氯化锂预处理均可导致肝组织糖原含量增加(P<0.05);尽管诱导脂多糖预处理并未改变GSK-3的蛋白表达水平(P>0.05),但导致GSK-3β抑制性磷酸化(P<0.05)和GSK-3α不完全裂解;大剂量脂多糖攻击后肝组织钙依赖蛋白酶活性较前显著升高(P<0.05),但组间比较无显著差异(P>0.05)。结论脂多糖预处理导致GSK-3β抑制性磷酸化和GSK-3α不完全裂解,促进肝组织糖原合成和聚集,但不影响钙依赖蛋白酶活性,有利于增加肝组织糖原储备并可能在遭受大剂量脂多糖攻击时提供能量需求。 展开更多
关键词 糖原合成酶激酶-3 糖原代谢 脂多糖 肝损伤 氯化锂 器官保护 GLYCOGEN SYNTHASE kinase-3
下载PDF
Molecular Cloning and Construction of agp Gene Deletion-mutant in Cyanobacterium Synechocystis sp. PCC 6803 被引量:1
6
作者 吴桂芳 沈忠耀 +1 位作者 吴庆余 赵南明 《Acta Botanica Sinica》 CSCD 2001年第5期512-516,共5页
The agp gene encoding the ADP-glucose pyrophosphorylase involved in cyanobacterial glycogen synthesis was amplified by PCR. The resulting agp fragment was cloned in plasmid pUC118 to generate plasmid pUCA. Part of the... The agp gene encoding the ADP-glucose pyrophosphorylase involved in cyanobacterial glycogen synthesis was amplified by PCR. The resulting agp fragment was cloned in plasmid pUC118 to generate plasmid pUCA. Part of the fragment within the agp DNA was deleted and replaced by an erythromycin resistance cassette to generate plasmid pUCAE, which was used to transform the Synechocystis sp. PCC 6803 wild-type strain and a mutant with resistance to erythromycin was obtained. PCR analysis of the genomic DNA from the resulting mutant indicated that the appropriate deletion and insertion indeed had occurred. The cell growth and Chl a, glycogen content in the mutant showed difference from those in the wild-type strain. The obtained biomass as well as the Chl a content in the mutant strain was higher than that of the wild-type strain, which suggested that the photosynthesis efficiency in the agp(-) strain was higher than that in the wild-type strain. No glycogen was found in the mutant, providing evidence for the correction of the mutant in physiological level. 展开更多
关键词 CYANOBACTERIUM Synechocystis sp PCC 6803 agp cloning deletion mutant glycogen synthesis photosynthesis
下载PDF
GSK3β、p-GSK3β^(ser9)、p-GSK3β^(Tyr216)在宫颈癌组织中的表达及临床意义 被引量:1
7
作者 陈良凤 张颖 +3 位作者 王仲奇 邸曼 郑福利 王建 《中国妇幼健康研究》 2015年第5期934-937,共4页
目的检测糖原合成酶激酶3β(GSK3β)、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达水平在宫颈癌组织中的表达情况及其与临床病理因素的相关性。方法收集2012年1月至2014年12月间第四军医大学西京医院妇产科收治的宫颈鳞癌病理标本80例,采... 目的检测糖原合成酶激酶3β(GSK3β)、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达水平在宫颈癌组织中的表达情况及其与临床病理因素的相关性。方法收集2012年1月至2014年12月间第四军医大学西京医院妇产科收治的宫颈鳞癌病理标本80例,采用免疫组化SP法对宫颈癌组织中GSK3β、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达水平进行检测,并分析GSK3β、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达与临床病理因素之间关系。结果宫颈癌组织中GSK3β、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达阳性率分别为18.8%、68.8%和12.5%。早期宫颈癌患者中GSK3β和p-GSK3β^(Tyr216)表达显著高于中晚期宫颈癌患者(25.5%vs.4.0%,χ~2=5.193,P=0.029;76.4%vs 52.0%,χ~2=4.749,P=0.039),早期宫颈癌患者中p-GSK3β^(ser9)表达显著低于中晚期宫颈癌患者(5.5%vs.28.0%,χ~2=7.988,P=0.009)。在宫颈癌组织中随着细胞学分级的降低,P-GSK3β^(ser9)阳性率也随之升高(7.4%、7.9%和33.3%,χ~2=7.329,P=0.031)。淋巴结转移阳性的宫颈癌患者中GSK3β和p-GSK3β^(Tyr216)表达均显著低于淋巴结转移阴性的宫颈癌患者(5.9%vs.28.3%,χ~2=6.427,P=0.018;52.9%vs.80.4%,χ~2=6.878,P=0.014),p-GSK3β^(ser9)在淋巴结转移阳性的宫颈癌患者中表达显著高于淋巴结转移阴性的宫颈癌患者(23.5%vs.4.3%,χ~2=6.577,P=0.015)。结论宫颈癌组织中GSK3β、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达与宫颈癌临床分期、细胞学分级和淋巴结转移相关,提示GSK3β基因可能在宫颈癌组织发生发展中发挥重要作用。 展开更多
关键词 宫颈 肿瘤 糖原合成酶激酶3Β 免疫组织化学 GLYCOGEN SYNTHASE KINASE 3β( GSK3β)
下载PDF
Changes and Correlations of Duroc Muscle pH,Glycogen,Lactic Acid and TBA under Different Storage Condition
8
作者 郭建凤 王继英 +4 位作者 蔺海朝 张印 呼红梅 王诚 武英 《Agricultural Science & Technology》 CAS 2015年第12期2795-2800,共6页
The changes and correlations of muscle pH, glycogen, lactic acid and in- tramuscular fat oxidation in Duroc pigs 10 d after their slaughter, and the effects of different storage temperature and time on Duroc muscle pH... The changes and correlations of muscle pH, glycogen, lactic acid and in- tramuscular fat oxidation in Duroc pigs 10 d after their slaughter, and the effects of different storage temperature and time on Duroc muscle pH value, water loss rate, glycogen, lactic acid and 2-thiobarbituric acid (TBA) were studied. The results showed that during the 10 h after the slaughter, the pH value was decreased rapid- ly, the lactic acid content was increased significantly, while the glycogen and TBA contents were remained stable. At the storage temperature of 4 ℃, storage time showed no significant effects on Duroc muscle pH value and glycogen, lactic acid and TBA contents. At the storage temperature of -20 ℃, storage temperature had significant effects on pH value, while no significant effects on other indicators. The correlation analysis demonstrated that during the 10 h after the slaughter, the TBA content was negatively related to glycogen content (P〈0.05), but positively related to lactic content (P〈0.05); the pH value was negatively related to lactic acid content (P〈0.05). At the storage temperature of 4 ℃, the TBA content was negatively relat- ed to water loss rate (P〈0.01) and lactic acid content (P〈0.05); the water loss rate was positively related to pH value (P〈0.01) and lactic acid content (P〈0.05). At the storage temperature of -20 ℃, the TBA content was negatively related to pH value (P〈0.01) and positively related to water loss rate (P〈0.05); the water loss rate was negatively related to pH value (P〈0.01) and lactic acid content (P〈0.05). 展开更多
关键词 DUROC Temperature Time pH value GLYCOGEN Lipid oxidation Corre- lation
下载PDF
糖原沉积病Ⅱ型合并脑干出血1例报告
9
作者 张炳蔚 雷阳 韩杰 《中风与神经疾病杂志》 CAS CSCD 北大核心 2014年第8期749-749,共1页
糖原沉积病(glycogen storage disease)是一类由于先天性酶缺陷所造成的糖原代谢障碍疾病,主要表现为进行性肌营养不良、骨骼肌无力,合并脑血管损害则较为罕见,国内未见报道。现将我院收治的糖原沉积病II型(Pompe病)合并脑干出血1... 糖原沉积病(glycogen storage disease)是一类由于先天性酶缺陷所造成的糖原代谢障碍疾病,主要表现为进行性肌营养不良、骨骼肌无力,合并脑血管损害则较为罕见,国内未见报道。现将我院收治的糖原沉积病II型(Pompe病)合并脑干出血1例报道如下。1临床资料患者,男性,17岁,因"突发头痛头晕、意识不清10 h"于2014年1月9日入院。 展开更多
关键词 糖原沉积病 脑干出血 先天性酶缺陷 GLYCOGEN 代谢障碍 肌无力 临床资料 安静状态 脑动脉 基底动脉
下载PDF
Wnt signaling control of bone cell apoptosis 被引量:30
10
作者 Bodine,PV 《Cell Research》 SCIE CAS CSCD 2008年第2期248-253,共6页
Wnts are a large family of growth factors that mediate essential biological processes like embryogenesis, morpho- genesis and organogenesis. These proteins also play a role in oncogenesis, and they regulate apoptosis ... Wnts are a large family of growth factors that mediate essential biological processes like embryogenesis, morpho- genesis and organogenesis. These proteins also play a role in oncogenesis, and they regulate apoptosis in many tissues. Wnts bind to a membrane receptor complex comprised of a frizzled (FZD) G-protein-coupled receptor and a low-density lipoprotein (LDL) receptor-related protein (LRP). The formation of this ligand-receptor complex initiates a number of signaling cascades that include the canonical/beta-catenin pathway as well as several noncanonical pathways. In recent years, canonical Wnt signaling has been reported to play a significant role in the control of bone formation. Clinical studies have found that mutations in LRP-5 are associated with reduced bone mineral density (BMD) and fractures. Investigations of knockout and transgenic mouse models of Wnt pathway components have shown that canonical Wnt signaling modulates most aspects ofosteoblast physiology including proliferation, differentiation, function and apoptosis. Transgenic mice expressing a gain of function mutant of LRP-5 in bone, or mice lacking the Wnt antagonist secreted frizzled-related protein-l, exhibit elevated BMD and suppressed osteoblast apoptosis. In addition, preclinical studies with pharmacologic compounds such as those that inhibit glycogen synthase kinase-3β support the importance of the canonical Wnt pathway in modulation of bone formation and osteoblast apoptosis. 展开更多
关键词 LDL receptor-related protein secreted frizzled-related protein glycogen synthase kinase OSTEOBLAST bone formation programmed cell death
下载PDF
Anti-diabetic activity of quercetin extracted from Phyllanthus emblica L.fruit: In silico and in vivo approaches 被引量:21
11
作者 Prabhu Srinivasan S.Vijayakumar +1 位作者 Swaminathan Kothandaraman Manogar Palani 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2018年第2期109-118,共10页
In this study, molecular interactions of the ligands, quercetin, gallic acid, and metformin with various diabetes mellitus-related protein targets, such as glycogen phosphorylase and peroxisome proliferatoractivated r... In this study, molecular interactions of the ligands, quercetin, gallic acid, and metformin with various diabetes mellitus-related protein targets, such as glycogen phosphorylase and peroxisome proliferatoractivated receptor gamma, were assessed. It was revealed that quercetin possesses good binding affinity to both targets. Quercetin is a major constituent of methanolic extracts of Phyllanthus emblica fruit. The antihyperglycemic effect of quercetin in streptozotocin(STZ)-induced diabetic rats was examined. The isolated quercetin administered at a dose of 75 mg/kg body weight produced a maximum decrease of14.78% in blood glucose levels in the diabetic rats after 7 days of treatment. Furthermore, quercetin doses of 50 and 75 mg/kg were shown to significantly improve the profiles of triglycerides, high-density lipoprotein, very-low-density lipoprotein, low-density lipoprotein, and total cholesterol at the end of the study in STZ-induced diabetic rats. The administration of quercetin(25, 50, and 75 mg/kg body weight)daily for 28 days in STZ-induced diabetic rats resulted in a significant decrease in blood glucose and urine sugar levels, with a considerable rise in plasma insulin and hemoglobin levels. Therefore, quercetin is a potential drug with antidiabetic and antihyperglycemic action mediated by changes in the levels of glucose, cholesterol, and triglycerides as indicated by in silico and in vivo studies. 展开更多
关键词 Bioactive molecules GLYCOGEN PHOSPHORYLASE Molecular docking PHYLLANTHUS emblica QUERCETIN ALBINO WISTER male rats
下载PDF
Glycogen storage diseases: New perspectives 被引量:33
12
作者 Hasan zen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第18期2541-2553,共13页
Glycogen storage diseases (GSD) are inherited metabolic disorders of glycogen metabolism. Different hormones, including insulin, glucagon, and cortisol regulate the relationship of glycolysis, gluconeogenesis and gl... Glycogen storage diseases (GSD) are inherited metabolic disorders of glycogen metabolism. Different hormones, including insulin, glucagon, and cortisol regulate the relationship of glycolysis, gluconeogenesis and glycogen synthesis. The overall GSD incidence is estimated 1 case per 20000-43000 live births. There are over 12 types and they are classified based on the enzyme deficiency and the affected tissue. Disorders of glycogen degradation may affect primarily the liver, the muscle, or both. Type I a involves the liver, kidney and intestine (and I b also leukocytes), and the clinical manifestations are hepatomegaly, failure to thrive, hypoglycemia, hyperlactatemia, hyperuricemia and hyperlipidemia. Type Ilia involves both the liver and muscle, and lib solely the liver. The liver symptoms generally improve with age. Type IV usually presents in the first year of life, with hepatomegaly and growth retardation. The disease in general is progressive to cirrhosis. Type Ⅵ and Ⅳ are a heterogeneous group of diseases caused by a deficiency of the liver phosphorylase and phosphorylase kinase system. There is no hyperuricemia or hyperlactatemia. Type Ⅺ is characterized by hepatic glycogenosis and renal Fanconi syndrome. Type Ⅱ is a prototype of inborn lysosomal storage diseases and involves many organs but primarily the muscle. Types V and Ⅶ involve only the muscle. 展开更多
关键词 Glycogen storage disease LIVER MUSCLE
下载PDF
Monitoring microbial community structure and succession of an A/O SBR during start-up period using PCR-DGGE 被引量:18
13
作者 WANG Xiuheng,ZHANG Kun,REN Nanqi,LI Nan,REN Lijiao State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China. 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2009年第2期223-228,共6页
Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) protocol was employed for revealing microbial community structure and succession in a sequential anaerobic and aerobic reactor perform... Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) protocol was employed for revealing microbial community structure and succession in a sequential anaerobic and aerobic reactor performing enhanced biological phosphorus removal (EBPR) during start-up period. High phosphorus removal was achieved after 15 d. On day 30, phosphorus removal efficiency reached to 83.2% and the start-up was finished. DGGE profiles of periodical sludge samples showed that dominant microbial species were 19 OTUs (operational taxonomy units). Unweighted pair-group method using arithmetic averages (UPGMA) clustering analysis revealed that rapid community succession correlated to low phosphorus removal rate and high phosphorus removal efficiency reflected on steady community structure. Sequencing results indicated that determined sequences (12 OTUs) belonged to Proteobacterium, Actinobacteria, Gemmatimonadales and unaffiliate group. Proteobacterium, Tetrasphaera elongate and Gemmatimonas aurantiaca may act important roles in phosphorus removal. With little amount as known glycogen accumulating organisms, Candidatus Competibacter phosphatis still at accumulating-phase had limited effect on microbial community structure. When climax community was obtained, dominant microbes were 14 OTUs. Microbes in a large amount were uncultured bacterium Thauera sp., uncultured y-Proteobacterium and Tetrasphaera elongata. 展开更多
关键词 biological phosphorus removal polyphosphate accumulating organisms glycogen accumulating organisms
下载PDF
Gastrointestinal complications of diabetes mellitus 被引量:13
14
作者 Babu Krishnan Shithu Babu +2 位作者 Jessica Walker Adrian B Walker Joseph M Pappachan 《World Journal of Diabetes》 SCIE CAS 2013年第3期51-63,共13页
Diabetes mellitus affects virtually every organ system in the body and the degree of organ involvement depends on the duration and severity of the disease,and other co-morbidities.Gastrointestinal(GI) involvement can ... Diabetes mellitus affects virtually every organ system in the body and the degree of organ involvement depends on the duration and severity of the disease,and other co-morbidities.Gastrointestinal(GI) involvement can present with esophageal dysmotility,gastro-esophageal reflux disease(GERD),gastroparesis,enteropathy,non alcoholic fatty liver disease(NAFLD) and glycogenic hepatopathy.Severity of GERD is inversely related to glycemic control and management is with prokinetics and proton pump inhibitors.Diabetic gastroparesis manifests as early satiety,bloating,vomiting,abdominal pain and erratic glycemic control.Gastric emptying scintigraphy is considered the gold standard test for diagnosis.Management includes dietary modifications,maintaining euglycemia,prokinetics,endoscopic and surgical treatments.Diabetic enteropathy is also common and management involves glycemic control and symptomatic measures.NAFLD is considered a hepatic manifestation of metabolic syndrome and treatment ismainly lifestyle measures,with diabetes and dyslipidemia management when coexistent.Glycogenic hepatopathy is a manifestation of poorly controlled type 1 diabetes and is managed by prompt insulin treatment.Though GI complications of diabetes are relatively common,awareness about its manifestations and treatment options are low among physicians.Optimal management of GI complications is important for appropriate metabolic control of diabetes and improvement in quality of life of the patient.This review is an update on the GI complications of diabetes,their pathophysiology,diagnostic evaluation and management. 展开更多
关键词 Gastrointestinal COMPLICATIONS Diabetes mellitus Esophageal COMPLICATIONS NONALCOHOLIC fatty liver disease DIABETIC GASTROPARESIS DIABETIC ENTEROPATHY Glycogenic HEPATOPATHY
下载PDF
Expression of PI3-K,PKB and GSK-3β in the skeletal muscle tissue of gestational diabetes mellitus 被引量:8
15
作者 Tao Zhang Min Fang +5 位作者 Zi-Mu Fu He-Chun Du Hua Yuan Gui-Yu Xia Jie Feng Gui-Yun Yin 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2014年第4期309-312,共4页
Objective:To analyze the expression of phosphatidylinositol 3 kinase(PI3-K),protein kinase B(PKB)and glycogen synthase kinase 3 beta(GSK-3β)in skeletal muscle tissue of gestational diabetes mellitus(GDM).Methods:A to... Objective:To analyze the expression of phosphatidylinositol 3 kinase(PI3-K),protein kinase B(PKB)and glycogen synthase kinase 3 beta(GSK-3β)in skeletal muscle tissue of gestational diabetes mellitus(GDM).Methods:A total of 90 cases of pregnant women were divided into observation group and control group according to the occurrence of GDM with 45 cases in either,and the expression of PI3-K,PKB,GSK-3βmRNA expression in skeletal muscle tissue was compared between two groups.Results:The total PI3-K p85 protein was significantly higher in the observation group compared with the control group,the activity of PI3-K was lower than that of the latter;The total PKB,GSK-3βprotein in skeletal tissue had no significant difference between two groups,while the serine phosphorylation levels of PKB and GSK-3βwere significantly lower in observation group compared with the control group.Conclusions:The downregulation of PI3-K,PKB and GSK-3βin skeletal tissue of GDM caused by phosphorylation dysfunction of signaling molecules is the reason for insulin resistance and transporter function decline which lead to GDM. 展开更多
关键词 PHOSPHATIDYLINOSITOL 3 KINASE Protein KINASE B GLYCOGEN synthase KINASE GESTATIONAL diabetes mellitus Skeletal musclet
下载PDF
Glycogen synthase kinase-3β positively regulates the proliferation of human ovarian cancer cells 被引量:15
16
作者 Qi Cao Xin Lu You-Ji Feng 《Cell Research》 SCIE CAS CSCD 2006年第7期671-677,共7页
Although glycogen synthase kinase-3 (GSK-3) might act as a tumor suppressor since its inhibition is expected to mimic the activation of Wnt-signaling pathway, GSK-3β may contribute to NF-κB activation in cancer ce... Although glycogen synthase kinase-3 (GSK-3) might act as a tumor suppressor since its inhibition is expected to mimic the activation of Wnt-signaling pathway, GSK-3β may contribute to NF-κB activation in cancer cells leading to increased cancer cell proliferation and survival. Here we report that GSK-3β activity was involved in the proliferation of human ovarian cancer cell both in vitro and in vivo. Inhibition of GSK-3 activity by pharmacological inhibitors suppressed proliferation of the ovarian cancer cells. Overexpressing constitutively active form of GSK-3β induced entry into the S phase, increased cyclin D1 expression and facilitated the proliferation of ovarian cancer cells. Furthermore, GSK-3 inhibition prevented the formation of the tumor in nude mice generated by the inoculation of human ovarian cancer cells. Our findings thus suggest that GSK-3β activity is important for the proliferation of ovarian cancer cells, implicating this kinase as a potential therapeutic target in ovarian cancer. 展开更多
关键词 glycogen synthase kinase ovarian cancer cell proliferation therapeutic target
下载PDF
GSK3β inhibits epithelial-mesenchymal transition via the Wnt/β-catenin and PI3K/Akt pathways 被引量:16
17
作者 Cheng Zhang Li Su +1 位作者 Li Huang Zheng-Yu Song 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第7期1120-1128,共9页
AIM: To investigate the regulatory mechanism of glycogen synthase kinase 3β(GSK3β) in epithelialmesenchymal transition(EMT) process after proliferative vitreoretinopathy(PVR) induction. METHODS: Experimenta... AIM: To investigate the regulatory mechanism of glycogen synthase kinase 3β(GSK3β) in epithelialmesenchymal transition(EMT) process after proliferative vitreoretinopathy(PVR) induction. METHODS: Experimental PVR was induced by intravitreal injection of retinal pigment epithelium(RPE) cells in the eyes of rabbits. A PI3 K/Akt inhibitor(wortmannin) and a GSK3β inhibitor(Li Cl) were also injected at different time during PVR progress. Electroretinogram(ERG), ocular fundus photographs, and B-scan ultrasonography were used to observe the PVR progress. Western blot test on the extracted retina were performed at 1, 2, 4 wk. The expression of the mesenchymal marker vimentin was determined by immunohistochemistry. Toxicity of wortmannin and Li Cl were evaluated by ERG and Td Tmediated d UTP nick-end labeling(TUNEL) assay. The vitreous was also collected for metabolomic analysis. RESULTS: Experimental PVR could significantly lead to EMT, along with the suppressed expression of GSK3β and the activation of Wnt/β-catenin and PI3 K/Akt pathways. It was verified that upregulating the expression of GSK3β could effectively inhibit EMT process by suppressing Wnt/β-catenin and PI3 K/Akt pathways. CONCLUSION: GSK3β effectively inhibits EMT via the Wnt/β-catenin and PI3 K/Akt pathways. GSK3β may be regarded as a promising target of experimental PVR inhibition. 展开更多
关键词 epithelial-mesenchymal transition experimental proliferative vitreoretinopathy glycogen synthase kinase RABBITS
下载PDF
Role of Microtubule-associated Protein Tau Phosphorylation in Alzheimer's Disease 被引量:14
18
作者 马荣红 张瑶 +3 位作者 洪小月 张俊菲 王建枝 刘恭平 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2017年第3期307-312,共6页
As a major microtubule-associated protein, tau plays an important role in promoting microtubule assembly and stabilizing microtubules. In Alzheimer’s disease(AD) and other tauopathies, the abnormally hyperphosphoryla... As a major microtubule-associated protein, tau plays an important role in promoting microtubule assembly and stabilizing microtubules. In Alzheimer’s disease(AD) and other tauopathies, the abnormally hyperphosphorylated tau proteins are aggregated into paired helical filaments and accumulated in the neurons with the form of neurofibrillary tangles. An imbalanced regulation in protein kinases and protein phosphatases is the direct cause of tau hyperphosphorylation. Among various kinases and phosphatases, glycogen synthase kinase-3β(GSK-3β) and protein phosphatase 2A(PP2A) are the most implicated. Accumulation of the hyperphosphorylated tau induces synaptic toxicity and cognitive impairments. Here, we review the upstream factors or pathways that can regulate GSK-3β or PP2A activity mainly based on our recent findings. We will also discuss the mechanisms that may underlie tau-induced synaptic toxicity. 展开更多
关键词 Alzheimer's disease TAU glycogen synthase kinase-3β protein phosphatase 2A synaptic toxicity
下载PDF
Sevoflurane plays a reduced role in cognitive impairment compared with isoflurane: limited effect on fear memory retention 被引量:9
19
作者 Ying Du Xiang-Dan Gong +3 位作者 Xin Fang Fang Xing Tian-Jiao Xia Xiao-Ping Gu 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第1期96-102,共7页
Isoflurane and sevoflurane are both inhalation anesthetics,but in clinical application,sevoflurane has been considered to be less suitable for long-term anesthesia because of its catabolic compounds and potential neph... Isoflurane and sevoflurane are both inhalation anesthetics,but in clinical application,sevoflurane has been considered to be less suitable for long-term anesthesia because of its catabolic compounds and potential nephrotoxicity.Nevertheless,recent studies have shown that these two inhalation anesthetics are similar in hepatorenal toxicity,cost,and long-term anesthetic effect.Moreover,sevoflurane possibly has less cognitive impact on young mice.In this study,C57BL/6 mice aged 8–10 weeks were exposed to 1.2%isoflurane or 2.4%sevoflurane for 6 hours.Cognitive function and memory were examined in young mice using the novel object recognition,contextual fear conditioning,and cued-fear extinction tests.Western blot assay was performed to detect expression levels of D1 dopamine receptor,catechol-O-methyltransferase,phospho-glycogen synthase kinase-3β,and total glycogen synthase kinase-3βin the hippocampus.Our results show that impaired performance was not detected in mice exposed to sevoflurane during the novel object recognition test.Contextual memory impairment in the fear conditioning test was shorter in the sevoflurane group than the isoflurane group.Long-term sevoflurane exposure did not affect memory consolidation,while isoflurane led to memory consolidation and reduced retention.Downregulation of hippocampal D1 dopamine receptors and phosphorylated glycogen synthase kinase-3β/total glycogen synthase kinase-3βand upregulation of catechol-O-methyltransferase may be associated with differing memory performance after exposure to isoflurane or sevoflurane.These results confirm that sevoflurane has less effect on cognitive impairment than isoflurane,which may be related to expression of D1 dopamine receptors and catechol-O-methyltransferase and phosphorylation of glycogen synthase kinase-3βin the hippocampus.This study was approved by the Institutional Animal Care and Use Committee,Nanjing University,China on November 20,2017(approval No.20171102). 展开更多
关键词 CATECHOL-O-METHYLTRANSFERASE cognitive impairment D1 dopamine receptors FEAR memory GLYCOGEN synthase kinase-3β ISOFLURANE NEUROTOXICOLOGY SEVOFLURANE
下载PDF
High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer's disease 被引量:19
20
作者 Chao-Chao Yu Ying Wang +4 位作者 Feng Shen Li-Hong Kong Ya-Wen Wang Hua Zhou Lei Tang 《Neural Regeneration Research》 SCIE CAS CSCD 2018年第10期1833-1841,共9页
Acupuncture has been shown to ameliorate cognitive impairment of Alzheimer’s disease.Acupoints and stimulation frequency influence the therapeutic effect of electroacupuncture.Rat models of Alzheimer’s disease were ... Acupuncture has been shown to ameliorate cognitive impairment of Alzheimer’s disease.Acupoints and stimulation frequency influence the therapeutic effect of electroacupuncture.Rat models of Alzheimer’s disease were established by injecting amyloid beta 1–42(Aβ_(1–42))into the bilateral lateral ventricles.Electroacupuncture at 2,30,and 50 Hz was carried out at Baihui(GV20;15°obliquely to a depth of 2mm)and Shenshu(BL23;perpendicularly to 4–6 mm depth),once a day for 20 minutes(each),for 15 days,taking a break every 7 days.The Morris water maze test was conducted to assess the learning and memory.The expression levels of glycogen synthase kinase-3β(GSK-3β),p Ser9-GSK-3β,p Tyr216-GSK-3β,amyloid precursor protein and Aβ_(1–40) in the hippocampus were determined by western blot assay.Results demonstrated that electroacupuncture treatment at different frequencies markedly improved learning and memory ability,increased synaptic curvatures,decreased the width of synaptic clefts,thickened postsynaptic densities,and downregulated the expression of GSK-3β,amyloid precursor protein,and Aβ_(1–40).pSer9-GSK-3βexpression markedly decreased,while p Tyr216-GSK-3βexpression increased.High-frequency(50 Hz)electroacupuncture was more effective than low(2 Hz)or medium-frequency(30 Hz)electroacupuncture.In conclusion,electroacupuncture treatment exerts a protective effect against Aβ_(1–42)-induced learning and memory deficits and synapse-ultrastructure impairment via inhibition of GSK-3βactivity.Moreover,high-frequency electroacupuncture was the most effective therapy. 展开更多
关键词 nerve regeneration ELECTROACUPUNCTURE different frequencies Alzheimer's disease cognitive impairment HIPPOCAMPUS glycogen synthase kinase-3β synaptic curvatures width of synaptic cleft postsynaptic density Baihui(GV20) Shenshu(BL23) neural regeneration
下载PDF
上一页 1 2 10 下一页 到第
使用帮助 返回顶部