Elevated fluid and glycosaminoglycan content in the Achilles tendon contribute to higher intratendinous pressures:Implications for Achilles tendinopathy

Background:Tendinopathy alters the compositional properties of the Achilles tendon by increasing fluid and glycosaminoglycan content.It has been speculated that these changes may affect intratendinous pressure,but the extent of this relationship remains unclear.Therefore,we aimed to investigate the impact of elevated fluid and glycosaminoglycan content on Achilles tendon intratendinous pressure and to determine whether hyaluronidase(HYAL) therapy can intervene in this potential relationship.Methods:Twenty paired fresh-frozen cadaveric Achilles tendons were mounted in a tensile-testing machine and loaded up to 5% strain.Intratendinous resting(at 0% strain) and dynamic pressure(at 5% strain) were assessed using the microcapillary infusion technique.First,intratendinous pressure was measured under native conditions before and after infusion of 2 mL physiological saline.Next,80 mg of glycosaminoglycans were administered bilaterally to the paired tendons.The right tendons were additionally treated with 1500 units of HYAL.Finally,both groups were retested,and the glycosaminoglycan content was analyzed.Results:It was found that both elevated fluid and glycosaminoglycan content resulted in higher intratendinous resting and dynamic pressures(p <0.001).HYAL treatment induced a 2.3-fold reduction in glycosaminoglycan content(p=0.002) and restored intratendinous pressures.Conclusion:The results of this study demonstrated that elevated fluid and glycosaminoglycan content in Achilles tendinopathy contribute to increased intratendinous re sting and dynamic pressures,which can be explained by the associated increased volume and reduced permeability of the tendon matrix,respectively.HYAL degrades glycosaminoglycans sufficiently to lower intratendinous pressures and may,therefore,serve as a promising treatment.

Quantitative detection and comparison of sulfate glycosaminoglycans content in extracellular matrix of in vitro cultured epiphyseal, articular and rib chondrocytes

Objective To establish a method for quantitative detection of the sulfate glycosaminoglycans ( GAG) content in extracellular matrix of in vitro cultured chondrocytes so as to evaluate the biological characteristics of epiphyseal, articular and rib chondrocytes. Methods Sulfate GAG content in extracellular matrix of three chondrocytes was measured by the modified dimethylmethylene blue (DMB) method. The changes of the toluidine blue (TB) stain of chondrocytes were observed by light microscope. Results Primary chondrocytes had the highest content of sulfate GAG in the extracellular matrix, ie, epiphyseal chondrocytes reached ( 70. 12 ± 7. 72 )μg/cm2, articular chondrocytes (92.00 ± 10.15) μg/cm2 and rib chondrocytes (80.61 ± 11. 40) μg/cm2, respectively. On the third pasage chondrocytes, epiphyceal chondrocytes decreased to (53.27 ± 9. 50 ) μg/cm2, articular chondrocytes to (63.88 ± 11.92) μg/cm2 and rib chondrocytes to (58.94 ±8.21) μg/cm2, respectively. The change of TB in every passage

Evaluation of Glycosaminoglycan in the Lumbar Disc Using Chemical Exchange Saturation Transfer MR at 3.0 Tesla:Reproducibility and Correlation with Disc Degeneration

Objective This study aims to explore the clinical applicability and relevance of giycosaminoglycan Chemical Exchange Saturation Transfer （gagCEST） for intervertebral disc. Methods 25 subjects ranging in age from 24 yrs to 74 yrs were enrolled, gagCEST was acquired using a single-slice TSE sequence on a 3T. Saturation used a continuous rectangular RF pulse with B1=0.8 I^T and a fixed duration time =1100 ms. Sagittal image was obtained firstly without saturation pulse, and then saturated images were acquired at 52 offsets ranging from ＋0.i25 to ＋_7 parts per million （ppm）. MR T2 relaxivity map was acquired at the identical location. Six subjects were scanned twice to assess scan-rescan reproducibility. Results GagCEST intraclass correlation coefficient （ICC） of six subjects was 0.759 for nucleus pulposus （NP） and 0.508 for annulus fibrosus （AF）. Bland-Altman plots showed NP had a mean difference of 0.10% （95% limits of agreement： -3.02% to 3.22%）; while that of AF was 0.34% （95% limits of agreement： -2.28% to 2.95%）. For the 25 subjects, gag CEST in NP decreased as disc degeneration increased, with a similar trend to T2 relaxivity. Gag CEST of AF showed a better correlation with disc degeneration than T2 relaxivity. Conclusion GagCEST in NP and AF decreased as disc degeneration increased, while gagCEST in AF showed a better correlation than T2 relaxivity.

Glycosaminoglycan remodeling during diabetes and the role of dietary factors in their modulation

Glycosaminoglycans(GAGs) play a significant role in various aspects of cell physiology.These are complex polymeric molecules characterized by disaccharides comprising of uronic acid and amino sugar.Compounded to the heterogeneity,these are variously sulfated and epimerized depending on the class of GAG.Among the various classes of GAG,namely,chondroitin/dermatan sulfate,heparin/heparan sulfate,keratan sulfate and hyaluronic acid(HA),only HA is non-sulfated.GAGs are known to undergo remodeling in various tissues during various pathophysiological conditions,diabetes mellitus being one among them.These changes will likely affect their structure thereby impinging on their functionality.Till date,diabetes has been shown to affect GAGs in organs such as kidney,liver,aorta,skin,erythrocytes,etc.to name a few,with deleterious consequences.One of the mainstays in the treatment of diabetes is though dietary means.Various dietary factors are known to play a significant role in regulating glucose homeostasis.Furthermore,in recent years,there has been a keen interest to decipher the role of dietary factors on GAG metabolism.This review focuses on the remodeling of GAGs in various organs during diabetes and their modulation by dietary factors.While effect of diabetes on GAG metabolism has been worked out quite a bit,studies on the role of dietary factors in their modulation has been few and far between.We have tried our best to give the latest reports available on this subject.

Enhancement of matrix metalloproteinases 2 and 9 accompanied with neurogenesis following collagen glycosaminoglycan matrix implantation after surgical brain injury

Surgical brain injury may result in irreversible neurological deficits. Our previous report showed that partial regeneration of a traumatic brain lesion is achieved by implantation of collagen glycosaminoglycan（CGM）. Matrix metalloproteinases（MMPs） may play an important role in neurogenesis but there is currently a lack of studies displaying the relationship between the stimulation of MMPs and neurogenesis after collagen glycosaminoglycan implantation following surgical brain trauma. The present study was carried out to further examine the expression of MMP2 and MMP9 after implantation of collagen glycosaminoglycan（CGM） following surgical brain trauma. Using the animal model of surgically induced brain lesion, we implanted CGM into the surgical trauma. Rats were thus divided into three groups：（1） sham operation group： craniotomy only;（2） lesion（L） group： craniotomy ＋ surgical trauma lesion;（3） lesion ＋ CGM（L ＋ CGM） group： CGM implanted following craniotomy and surgical trauma lesion. Cells positive for SOX2（marker of proliferating neural progenitor cells） and matrix metalloproteinases（MMP2 and MMP9） in the lesion boundary zone were assayed and analyzed by immunofluorescence and ELISA commercial kits, respectively. Our results demonstrated that following implantation of CGM after surgical brain trauma, significant increases in MMP2^＋/SOX2^＋ cells and MMP9^＋/SOX2^＋ cells were seen within the lesion boundary zone in the L ＋ CGM group. Tissue protein concentrations of MMP2 and MMP9 also increased after CGM scaffold implantation. These findings suggest that implantation of a CGM scaffold alone after surgical brain trauma can enhance the expression of MMP2 and MMP9 accompanied by neurogenesis.

Research progress on the skincare efficacy of sulfated glycosaminoglycans

With the improvement of consumers’scientific skin care consciousness,skin problems-oriented efficacy skin care and precise skin care methods have become the future development trend.Glycosaminoglycans are long,linear polysaccharides comprised of repeated disaccharide units,which are highly expressed endogenously in the skin.They are commonly present in skin cells,cell surface and extracellular matrix,with pleiotropic biological function.In addition to hyaluronic acid,the sulfated glycosaminoglycans heparin,heparan sulfate,dermatan sulfate and chondroitin sulfate play an important role in anti-wrinkle,firming,soothing,and improving microvascular circulation,but are still in the research and development stage in cosmetics.This paper summarizes the skincare mechanism of sulfated glycosaminoglycans,and demonstrates the potential of sulfated glycosaminoglycans as functional raw materials in cosmetics.

Assessment of Glycosaminoglycan Content of Lumbar Intervertebral Discs in Patients with Radiculopathy

Objective: To assess glycosaminoglycan (GAG) content of lumbar intervertebral discs (IVDs) in patients with radiculopathy compared with healthy volunteers with glycosaminoglycan chemical exchange saturation transfer (gagCEST). Methods: The lumbar spines of 15 patients with radiculopathy (9 women, 6 men;mean age 45 years;range: 19 - 80 years) and 13 healthy controls (10 women, 3 men;mean age 29 years;range: 19 - 38 years) without lumbar back pain or previous spine surgery were examined at a 3 Tesla (T) magnetic resonance imaging (MRI) scanner in this prospective study. The MRI protocol included standard morphological, sagittal, and transverse T2-weighted (T2w) images of the five lumbar IVDs (L1-S1) to assess Pfirrmann score and to detect disc disorders according to the Combined Task Force classification. To analyze biochemically the lumbar IVDs, a gagCEST sequence was applied to measure the GAG content of the nucleus pulposus (NP) and annulus fibrosus (AF). Results: Patients with radiculopathy indicated significantly lower gagCEST values in NP than healthy volunteers (2.82% &plusmn;3.12% vs. 4.09% &plusmn;2.25%, P = 0.017). The GAG content of AF showed no significant difference between volunteers and patients (2.66% &plusmn;2.01% vs. 1.92% &plusmn;2.56%;P = 0.175). Conclusions. Patients with radiculopathy presented with lower GAG values than healthy volunteers in NP, indicating an association between pain and IVD degeneration. gagCEST of lumbar IVDs is a powerful, non-invasive tool to investigate early disc degeneration, which we could demonstrate in the NP in our study collective.

Preparation and Biocompatibility of Porous Poly(vinylalcohol)-Glycosaminoglycan-Collagen Scaffold

This paper aims to prepare a PVA-GAG-COL composite with polyvinyl alcohol (PVA), glycosaminoglycan (GAG) and collagen (COL) by the method of freeze drying and to investigate the feasibility as a tissue engineering scaffold for tissue or organ repairing. In this study, SEM was used to observe the morphology. Biocompatibility was tested by cell culture with the extracted fluid of composite materials. Different proportional scaffolds could be obtained with different concentrations and alcoholysis degree of PVA. Different proportional scaffolds also had different porous structures. SEM proved that large amount of porous structure could be formed. Biocompatibility test showed that the extracted fluid of composite materials was nontoxic, which could promote the adhesion and proliferation of the fibroblast. Fibroblast could grow on the scaffold normally.A porous scaffold for tissue engineering with high water content can be fabricated by PVA, GAG and COL, which has excellent cell biocompatibility. The porous structure shows potential in tissue engineering and cell culture.

Polyvinyl Alcohol-Collagen Composite with Glycosaminoglycan as Scaffolds for Tissue Engineering

The aim of this study is to prepare a PVA-GAG-COL composite material by polyvinyl alcohol (PVA),glycosaminoglycan (GAG) and collagen (COL),and to investigate the feasibility of serving as a scaffold for tissue engineering. PVA was blended with various amounts of GAG and COL. Different proportional scaffolds could be obtained with different molecular weight and alcoholysis degree of PVA and different amounts of GAG,which exhibited high water content (60%-95%) and showed different inner configuration with swelling ratio (120%-620%). SEM proved that different composite materials had different porous structures.

Antiviral Effects of Stichopus japonicus Acid Mucopolysaccharide on Hepatitis B Virus Transgenic Mice

Hepatitis B virus(HBV) is a significant global pathogen and efficient cure for HBV patients is still a challenging goal. We previously reported that acidic mucopolysaccharide from stichopus japonicus selenka(SJAMP) could inhibit HBs Ag and HBe Ag expression in vitro. However, the potential anti-HBV effects of SJAMP in vivo have not yet been explored. In this study, we show that SJAMP exhibits potent anti-HBV activity in HBV transgenic mice in a dose-dependent manner. Specifically, sixty HBV transgenic male BALB/c mice were randomly selected to receive the treatment of PBS, low dose SJAMP(30 mg kg^(-1)), middle dose SJAMP(40 mg kg^(-1)), high dose SJAMP(50 mg kg^(-1)) and IFN(45 IU kg^(-1)) for 30 d. SJAMP treatment suppressed serum HBV-DNA, and liver HBs Ag and HBc Ag levels in HBV-transgenic mice. The present study highlights the potential application of SJAMP in HBV therapy.

Pores Created by Laser Surface Modification of Poly(vinylalcohol)-Collagen with Glycosaminoglycan Scaffold for Cell Culture in Tissue Engineering

A PVA-GAG-COL composite scaffold is fabricated by polyvinyl alcohol （PVA）, glyeosaminoglycan （GAG） and collagen （COL）. Laser surface modification technology is used to make holes on the surface of the scaffolds. Inside and outside interconnection micro-porous structure is obtained. Bioeompatibility test of the scaffolds shows that PVA-GAG-COL scaffold can promote the adhesion and proliferation of the fibroblast. Also, fibroblast can grow normally on the scaffolds with pore diameter from 115 um to 255 um and pore distance from 500 um to 2000 um. PVA-GAG-COL scaffolds possess excellent cell biocompatibility. The porous structure is suitable for cell culture in tissue engineering.

0.03%他克莫司软膏联合多磺酸粘多糖乳膏治疗面部单纯糠疹的效果观察

目的:观察探讨0.03%他克莫司软膏联合多磺酸粘多糖乳膏治疗面部单纯糠疹的美学效果。方法:选取2019年11月-2022年1月门诊收治的105例面部单纯糠疹患儿为观察对象,通过随机数字法随机分为联合组(n=53)和对照组(n=52),对照组给予多磺酸粘多糖乳膏治疗,联合组在对照组基础上加用0.03%他克莫司软膏治疗,比较两组治疗后的临床疗效、症状体征评分、皮肤生理功能指标[角质层含水量(Water content of stratum corneum,WCSC)、皮肤油脂(Skin sebum,SC)、经表皮水分流失(Transepidermal water loss,TEWL)]、复发情况、不良反应及美学效果满意度。结果:治疗4周后,联合组总有效率为88.68%,对照组为71.15%,差异有统计学意义(P<0.05)。治疗前两组患儿各项症状体征评分差异均无统计学意义(P>0.05);治疗4周后,两组患儿鳞屑、瘙痒、色素减退、皮损面积及皮损数量评分均较治疗前显著降低(P<0.05),且联合组各症状体征评分明显低于对照组(P<0.05);治疗前两组患儿皮肤生理功能指标差异均无统计学意义(P>0.05);治疗4周后,两组患儿WCSC和SC均显著升高(P<0.05),TEWL均显著降低(P<0.05),且联合组改善优于对照组(P<0.05);结束治疗随访4周,联合组患儿复发率(14.89%)低于对照组(27.03%),差异无统计学意义(P>0.05);治疗期间,两组患儿均未出现明显不良反应;联合组患儿美学效果总满意度(96.23%)显著高于对照组(82.69%)(P<0.05)。结论:0.03%他克莫司软膏联合多磺酸粘多糖乳膏治疗面部单纯糠疹疗效显著,可有效改善患儿皮损症状和皮肤生理功能,能有效预防复发,提高美学效果,且安全性好,值得临床推广应用。

中药热敷联合多磺酸粘多糖乳膏治疗动静脉内瘘血肿的效果观察

目的探讨中药热敷联合多磺酸粘多糖乳膏对动静脉内瘘血肿的护理效果。方法本研究为随机对照试验。选取2021年2月至2023年2月连云港市中医院门诊动静脉内瘘血肿患者60例,按照随机数字表法分为参照组30例和联合组30例。参照组男21例,女9例,年龄(47.58±7.15)岁,采用多磺酸粘多糖乳膏联合常规护理;联合组男20例,女10例,年龄(46.45±6.14)岁,在参照组基础上实施中药热敷。记录并比较两组患者的皮下血肿消退时间和疼痛持续时间、并发症发生情况、护理满意度。采用t检验、χ^(2)检验。结果联合组患者的皮下血肿消退时间、疼痛持续时间均短于参照组[(7.54±2.25)d比(10.85±3.14)d、(4.25±0.48)d比(6.50±0.51)d],差异均有统计学意义(t=6.934、9.072,均P<0.001);联合组患者的并发症发生率低于参照组[6.67%(2/30)比26.67%(8/30)],护理总满意度高于参照组[90.00%(27/30)比66.67%(20/30)],差异均有统计学意义(χ^(2)=4.320、4.812,P=0.038、0.028)。结论中药热敷联合多磺酸粘多糖乳膏可加快动静脉内瘘血肿患者的皮下血肿消退,缩短疼痛持续时间,提升护理满意度,减少并发症发生,缓解炎症,值得临床推广。

多磺酸粘多糖乳膏联合光子治疗仪治疗化学性静脉炎的临床疗效

目的研究化学性静脉炎患者采用多磺酸粘多糖乳膏联合光子治疗的效果。方法选取2022年1月—2023年6月重庆市中医院收治的108例化学性静脉炎患者为研究对象,按随机数字表法将其分为对照组和观察组,每组54例。对照组采用多磺酸粘多糖乳膏治疗,观察组在对照组基础上采用光子治疗。比较两组患者的治疗效果。结果观察组的静脉弹性恢复及红肿消退时间均短于对照组,组间差异有统计学意义(P<0.05)。治疗后,观察组的疼痛数字评价量表评分低于对照组,差异有统计学意义(P<0.05)。观察组的治疗总有效率、治疗总满意率分别为98.15%、100.00%,均高于对照组的85.19%、83.33%,组间差异有统计学意义(P<0.05)。观察组的不良反应发生率为1.85%,低于对照组的18.52%,差异有统计学意义(P<0.05)。结论化学性静脉炎患者采用多磺酸粘多糖乳膏联合光子治疗的效果显著,可快速缓解患者症状,减轻其疼痛,且不良反应发生率较低,值得临床推广使用。

液相色谱串联质谱检测尿黏多糖在黏多糖贮积症患者诊断与随访中的应用

目的运用液相色谱串联质谱(LC-MS/MS)技术建立尿液中硫酸软骨素(CS)、硫酸皮肤素(DS)和硫酸乙酰肝素(HS)含量的检测方法,探究该方法在黏多糖贮积症(MPS)患者诊断及治疗随访中的应用。方法收集20例MPS患儿及37例正常儿童尿液样本,测定尿液中CS、DS和HS等浓度。尿样氮气吹干,盐酸甲醇衍生化,再次氮气吹干,复溶后LC-MS/MS分析;评价方法的线性、定量限、精密度、加样回收率和基质效应。结果LC-MS/MS检测CS、DS和HS的线性均大于0.99。CS、DS和HS的定量下限依次为:0.5、1.0和1.0 mg/L。批内不精密度为1.9%~10.4%,批间不精密度为2.6%~9.8%。加标回收率为85.6%~110.4%,相对基质效应为84.9%~115.5%。CS、DS和HS在正常儿童尿液中呈正态分布,以x+1.64 SD计算正常儿童尿液中的参考区间上限,分别为16.5、1.8、1.4 mg/mmol。通过分析MPS患儿尿液黏多糖特点,LC-MS/MS法能有效检出MPS I、MPSⅡ、MPSⅢ和MPSⅥ等MPS患儿。已接受造血干细胞移植的MPSⅠ和MPSⅡ患儿尿DS、HS水平降低。结论LC-MS/MS检测尿黏多糖性能良好,有望用于MPS患者的精准诊断和治疗随访监测。

硫酸软骨素蛋白多糖在脊髓损伤中的研究进展

硫酸软骨素蛋白多糖(chondroitin sulfate proteoglycans,CSPGs)是中枢神经系统细胞外基质的组成部分,在中枢神经系统发育、正常维持及病理过程中都发挥着关键的作用。脊髓损伤后,损伤部位CSPGs的表达明显上调,这主要源于病变部位活化的星形胶质细胞。CSPGs的上调会限制脊髓损伤部位的轴突再生、传导和再髓鞘化,并且可以促进脊髓损伤中的炎症反应,不利于脊髓损伤后神经功能恢复。因此,抑制CSPGs可能是促进脊髓损伤后轴突再生和功能恢复的有效治疗方法。本文对CSPGs在脊髓损伤中的研究现状进行综述。

多磺酸粘多糖乳膏联合阿达帕林凝胶治疗玫瑰痤疮的疗效及对皮肤屏障功能的影响

目的:研究多磺酸粘多糖乳膏联合阿达帕林凝胶治疗玫瑰痤疮的疗效及对皮肤屏障功能的影响。方法:选取2019年8月-2021年8月笔者医院126例玫瑰痤疮患者进行前瞻性随机对照试验,以随机数字表法将患者均分为观察组和对照组,各63例,两组均给予盐酸多西环素片口服同时采用阿达帕林凝胶局部外涂治疗,观察组患者另加用多磺酸粘多糖乳膏治疗,两组疗程均为8周。比较两组治疗有效率,治疗后皮肤屏障功能、角质层分析结果和炎症反应水平。结果:治疗8周后,观察组治疗有效率为85.71%,高于对照组(68.25%),差异有统计学意义(P<0.05);治疗8周后,两组角质层含水量和表皮油脂含量均明显升高(P<0.05),两组经表皮失水率(Transepidermal water loss,TEWL)和红斑指数(Erythema index,EI)明显降低(P<0.05),且观察组角质层含水量和表皮油脂含量高于对照组,TEWL和EI低于对照组,差异有统计学意义(P<0.05);治疗8周后,两组皮肤粘脱蛋白质含量、丝氨酸蛋白酶活性及血清抗菌肽LL-37、白介素6(Interleukin 6,IL-6)、基质金属蛋白酶9(Matrix metalloproteinase 9,MMP-9)水平均较治疗前明显降低(P<0.05),且观察组各项指标均低于对照组,差异有统计学意义(P<0.05)。结论:多磺酸粘多糖乳膏和阿达帕林凝胶联合治疗丘疹脓疱型玫瑰痤疮可有效改善局部炎症反应,减轻皮肤组织损伤,促进皮肤屏障功能恢复,提升治疗效果。

超脉冲二氧化碳点阵激光联合多磺酸粘多糖乳膏治疗凹陷性痤疮瘢痕的临床效果

目的分析超脉冲二氧化碳点阵激光与多磺酸粘多糖联合治疗凹陷性痤疮瘢痕的临床效果。方法研究选取我院2022年10月-2023年10月临床收治凹陷性痤疮瘢痕患者60例,随机分组为对照组(30人)与治疗组(30人),所有患者均接受超脉冲二氧化碳点阵激光治疗,观察组在治疗中联合多磺酸粘多糖乳膏治疗,对比组间治疗效果。结果治疗后,接受治疗组ECCA评分相较于对照组更低(P<0.05);治疗组反应发生率低于对照组(3.33%VS 20.00%)(P<0.05);治疗组治疗6个月后临床治疗有效率显著高于对照组(χ2=7.925,P<0.05)。结论激光联合药物治疗凹陷性痤疮瘢痕的疗效更佳,能显著降低患者痤疮瘢痕症状且不良反应发生率低。

糖胺聚糖在骨组织工程中的应用进展

骨组织缺损是目前最常见的医疗疾病之一。自体骨移植和同种异体骨移植等存在供体不足、移植物免疫反应及移植部位感染等不足,而骨组织工程(BTE)可通过合适的骨替代支架克服目前骨组织重建方法的局限性。骨支架材料应具有生物相容性、生物可再生性和仿生性。天然来源的糖胺聚糖(GAG)可模拟哺乳动物细胞外基质成分,具有稳定和持续释放生长因子、可调的孔隙率、磷酸钙成核位点、一定的黏弹性和保水性。因此,GAG具有调节生物矿化、稳定生长因子、促进细胞黏附及细胞信号转导等功能,可作为一种独特的生物材料广泛用于BTE。

多磺酸粘多糖乳膏联合京万红软膏治疗Ⅰ、Ⅱ期压疮的效果观察

目的探讨多磺酸粘多糖乳膏联合京万红软膏在治疗Ⅰ、Ⅱ期压疮的效果。方法选择2022年4月至2023年9月在本院骨科收治的带入Ⅰ、Ⅱ期压疮患者60例参与研究,按照随机数字表法分为试验组与对照组,每组30例,对照组使用传统换药治疗,试验组采用多磺酸粘多糖乳膏联合京万红软膏治疗。对比两组治疗后的临床疗效、临床指标、治疗舒适度、治疗前后创面恢复及生活质量的差异性。结果对照组治疗有效率为63.33%(19/30),试验组的治疗有效率为86.67%(26/30),试验组的治疗有效率高于对照组,差异具有统计意义(P<0.05);试验组在水泡吸收时间、创面结痂时间、压疮愈合时间、换药次数均低于对照组,差异具有统计意义(P<0.05);对照组治疗舒适度为66.67%(20/30),试验组的治疗舒适度为90.00%(27/30),试验组的治疗舒适度高于对照组(P<0.05);两组在治疗前,PUSH评分和WHOQOL—Bref评分差异无统计学意义(P>0.05);治疗后,两组PUSH评分均比治疗前有所降低,WHOQOL—Bref评分有所升高,且试验组PUSH评分低于对照组,WHOQOL—Bref评分高于对照组(P<0.05)。结论多磺酸粘多糖乳膏联合京万红软膏治疗Ⅰ、Ⅱ期压疮中发挥良好的功效,能提高临床的治疗效果,促进伤口愈合,提高患者治疗的舒适感和生活质量。