Objective:To evaluate the therapeutic potential of hydroalcoholic extract of licorice root against ethanol and cerulein induced chronic pancreatitis in rats.Methods:The phytochemical profile of hydroalcoholic extract ...Objective:To evaluate the therapeutic potential of hydroalcoholic extract of licorice root against ethanol and cerulein induced chronic pancreatitis in rats.Methods:The phytochemical profile of hydroalcoholic extract of licorice root was determined by high performance liquid chromatography(HPLC)and gas chromatography coupled to mass spectrometry(GC-MS).Chronic pancreatitis was induced in male albino Wistar rats by feeding them a diet containing ethanol(0%-36%of total calories)for 4 weeks and cerulein(20μg/kg b.wt,i.p.)thrice a week for 3 weeks.Lipase and amylase in serum,lipid peroxides and antioxidants including reduced glutathione,glutathione peroxidase,superoxide dismutase and catalase in pancreas were determined.Inflammatory response was measured by myeloperoxidase in the pancreas,caspase-1 and the concentrations of IL-1 as and liver was carried oαand IL-18 in serum.Moreover,histological evaluation of the pancreut.Results:Different flavonoids and saponins were identified in the hydroalcoholic extract of licorice root through HPLC and GC-MS.A marked increase in the levels of serum lipase,amylase,lipid peroxides,caspase-1,myeloperoxidase,IL-1 in the levels of antioxidants were observed after ethanoα,and IL-18 and a marked decrease l and cerulein administration.Treatment with hydroalcoholic extract of licorice root attenuated these changes.In addition,histological observation confirmed the protective effect of the extract in the pancreas and liver against inflammatory changes induced by ethanol and cerulein.Conclusions:The licorice root extract attenuates ethanol and cerulein induced pancreatitis in rats probably due to its antioxidant phytonutrients since ethanol and cerulein-induced production of reactive oxygen species contributes to severe inflammation in the pancreas.展开更多
An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry （UPLC-qTOF-MS/MS） method was developed and validated for the simultaneous determination of glycyrrhizin and glycyrrhetic acid. T...An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry （UPLC-qTOF-MS/MS） method was developed and validated for the simultaneous determination of glycyrrhizin and glycyrrhetic acid. These analytes were separated on a reverse phase C18 column using a mobile phase of acetonitrile：2% acetic acid in water （75：25, v/v） with a flow rate of 200 μL/min. The qTOF-MS was operated under multiple reaction monitoring （MRM） mode using the electrospray ionization （ESI） technique with positive ion polarity. A comparison of three different extraction techniques i.e. accelerated solvent extraction （ASE）, extraction under ultrasonic waves （USW） and the classical extraction by percolation （CE） method was done and quantification of these extracts was also carried out by the proposed method.展开更多
Glycyrrhiza glabra L.is the most widely used herb in the ancient history of Ayurvedic medicine,as a medicinal value as well as an aromatic herb,and it is commonly known as Mulhatti.Mulhatti roots are useful for medica...Glycyrrhiza glabra L.is the most widely used herb in the ancient history of Ayurvedic medicine,as a medicinal value as well as an aromatic herb,and it is commonly known as Mulhatti.Mulhatti roots are useful for medically and are also a good source of phytoproducts and secondary metabolites present in Mulhatti roots are triterpenoid saponin,glycosides,glycyrrhizin,prenylated biaurone,licoaagrone,7-acetoxy-2-methylisoflavone,4-methylcoumarin,liqcoumarin,glycyrrhetinic acid,quercetin,liquiritigenin,isoliquiritigenin,etc.This study was carried out to study the evaluation of phenolic compounds,2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical activity and general antioxidant capacity of water extracts of Mulhatti roots prepared at different pH values,namely 2,4,7 and 9.Data have shown great differences in terms of results.Most of the phenolic compounds are at pH 7(19.25),followed by pH 9(17.25),pH 2(14.62)and pH 4(8.89 mg GAE/g),respectively.Similarly,the flavonoid data also showed variations,the maximum has been present in pH 2(5.39),then pH 7(3.02),pH 9(1.79)and pH 4(1.40 mg CE/g),respectively.The value for DPPH IC50 free radical scavenging activity was the lowest at pH 7(82.22),followed by pH 2(110.40),pH 4(111.99)and pH 9(146.24μg/mL)and IC50 reference standard(ascorbic acid)was 59.52μg/mL in distilled water.The total capacity of the antioxidant was the highest at pH 2(9.93)followed by pH 4(5.54),pH 7(5.34)and pH 9(4.23 mg AAE/g).According to current research,pH 7 is the best for phytochemicals as well as antioxidants that catch harmful radicals.展开更多
Background: Siddha system is one of the ancient medical systems which originated in Indiamore than 4000 years ago had given many formularies and treatment methods for variousdiseases especially skin diseases and autoi...Background: Siddha system is one of the ancient medical systems which originated in Indiamore than 4000 years ago had given many formularies and treatment methods for variousdiseases especially skin diseases and autoimmune diseases. One of the most well-knownmembers of the Fabaceae family and a popular medicinal plant is Athimathuram(Glycyrrhiza glabra). Glycyrrhiza glabra root powder (Athimathuram chooranam) fromSiddha herbal formulation mentioned in the texts to treat vitiligo. The study is aimed toexecute the In Silico computational studies of phytoconstituents of Siddha herbAthimathuram chooranam (Glycyrrhiza glabra) against Tyrosinase enzyme for the treatmentof vitiligo. Method: Making use of Auto Dock 4, docking calculations were performed.Gasteiger partial charges were applied to the ligand atoms. In order to simulate docking, theSolis & Wets local search approach and the Lamarckian genetic algorithm were employed.The ligand molecules’ initial locations, orientations, and torsion angles were determined atrandom. During docking, all rotatable torsions were freed. Results: By interacting withamino acids located on the active site of the tyrosinase enzyme, bioactive substances likeGlycyrrhizin, Liquirtin, Glabridin, Umbelliferone, and Glabrene included in the Siddhaformulation exhibit substantial binding activity against the target protein. Conclusion: Itwas concluded that these compounds might exert promising anti-vitiligo properties.展开更多
文摘Objective:To evaluate the therapeutic potential of hydroalcoholic extract of licorice root against ethanol and cerulein induced chronic pancreatitis in rats.Methods:The phytochemical profile of hydroalcoholic extract of licorice root was determined by high performance liquid chromatography(HPLC)and gas chromatography coupled to mass spectrometry(GC-MS).Chronic pancreatitis was induced in male albino Wistar rats by feeding them a diet containing ethanol(0%-36%of total calories)for 4 weeks and cerulein(20μg/kg b.wt,i.p.)thrice a week for 3 weeks.Lipase and amylase in serum,lipid peroxides and antioxidants including reduced glutathione,glutathione peroxidase,superoxide dismutase and catalase in pancreas were determined.Inflammatory response was measured by myeloperoxidase in the pancreas,caspase-1 and the concentrations of IL-1 as and liver was carried oαand IL-18 in serum.Moreover,histological evaluation of the pancreut.Results:Different flavonoids and saponins were identified in the hydroalcoholic extract of licorice root through HPLC and GC-MS.A marked increase in the levels of serum lipase,amylase,lipid peroxides,caspase-1,myeloperoxidase,IL-1 in the levels of antioxidants were observed after ethanoα,and IL-18 and a marked decrease l and cerulein administration.Treatment with hydroalcoholic extract of licorice root attenuated these changes.In addition,histological observation confirmed the protective effect of the extract in the pancreas and liver against inflammatory changes induced by ethanol and cerulein.Conclusions:The licorice root extract attenuates ethanol and cerulein induced pancreatitis in rats probably due to its antioxidant phytonutrients since ethanol and cerulein-induced production of reactive oxygen species contributes to severe inflammation in the pancreas.
文摘An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry （UPLC-qTOF-MS/MS） method was developed and validated for the simultaneous determination of glycyrrhizin and glycyrrhetic acid. These analytes were separated on a reverse phase C18 column using a mobile phase of acetonitrile：2% acetic acid in water （75：25, v/v） with a flow rate of 200 μL/min. The qTOF-MS was operated under multiple reaction monitoring （MRM） mode using the electrospray ionization （ESI） technique with positive ion polarity. A comparison of three different extraction techniques i.e. accelerated solvent extraction （ASE）, extraction under ultrasonic waves （USW） and the classical extraction by percolation （CE） method was done and quantification of these extracts was also carried out by the proposed method.
文摘Glycyrrhiza glabra L.is the most widely used herb in the ancient history of Ayurvedic medicine,as a medicinal value as well as an aromatic herb,and it is commonly known as Mulhatti.Mulhatti roots are useful for medically and are also a good source of phytoproducts and secondary metabolites present in Mulhatti roots are triterpenoid saponin,glycosides,glycyrrhizin,prenylated biaurone,licoaagrone,7-acetoxy-2-methylisoflavone,4-methylcoumarin,liqcoumarin,glycyrrhetinic acid,quercetin,liquiritigenin,isoliquiritigenin,etc.This study was carried out to study the evaluation of phenolic compounds,2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical activity and general antioxidant capacity of water extracts of Mulhatti roots prepared at different pH values,namely 2,4,7 and 9.Data have shown great differences in terms of results.Most of the phenolic compounds are at pH 7(19.25),followed by pH 9(17.25),pH 2(14.62)and pH 4(8.89 mg GAE/g),respectively.Similarly,the flavonoid data also showed variations,the maximum has been present in pH 2(5.39),then pH 7(3.02),pH 9(1.79)and pH 4(1.40 mg CE/g),respectively.The value for DPPH IC50 free radical scavenging activity was the lowest at pH 7(82.22),followed by pH 2(110.40),pH 4(111.99)and pH 9(146.24μg/mL)and IC50 reference standard(ascorbic acid)was 59.52μg/mL in distilled water.The total capacity of the antioxidant was the highest at pH 2(9.93)followed by pH 4(5.54),pH 7(5.34)and pH 9(4.23 mg AAE/g).According to current research,pH 7 is the best for phytochemicals as well as antioxidants that catch harmful radicals.
文摘Background: Siddha system is one of the ancient medical systems which originated in Indiamore than 4000 years ago had given many formularies and treatment methods for variousdiseases especially skin diseases and autoimmune diseases. One of the most well-knownmembers of the Fabaceae family and a popular medicinal plant is Athimathuram(Glycyrrhiza glabra). Glycyrrhiza glabra root powder (Athimathuram chooranam) fromSiddha herbal formulation mentioned in the texts to treat vitiligo. The study is aimed toexecute the In Silico computational studies of phytoconstituents of Siddha herbAthimathuram chooranam (Glycyrrhiza glabra) against Tyrosinase enzyme for the treatmentof vitiligo. Method: Making use of Auto Dock 4, docking calculations were performed.Gasteiger partial charges were applied to the ligand atoms. In order to simulate docking, theSolis & Wets local search approach and the Lamarckian genetic algorithm were employed.The ligand molecules’ initial locations, orientations, and torsion angles were determined atrandom. During docking, all rotatable torsions were freed. Results: By interacting withamino acids located on the active site of the tyrosinase enzyme, bioactive substances likeGlycyrrhizin, Liquirtin, Glabridin, Umbelliferone, and Glabrene included in the Siddhaformulation exhibit substantial binding activity against the target protein. Conclusion: Itwas concluded that these compounds might exert promising anti-vitiligo properties.
