文章采用超支化聚合物聚酰胺胺(HPAMAM)和氧化石墨烯(graghene oxide,GO),通过真空抽滤自组装的方法制备了仿贝壳复合材料,并对其用京尼平进行了适当的交联。采用透射电子显微镜(transmission electron microscope,TEM)对GO进行了表征,...文章采用超支化聚合物聚酰胺胺(HPAMAM)和氧化石墨烯(graghene oxide,GO),通过真空抽滤自组装的方法制备了仿贝壳复合材料,并对其用京尼平进行了适当的交联。采用透射电子显微镜(transmission electron microscope,TEM)对GO进行了表征,采用红外光谱(infrared spectroscopy,IR)、扫描电子显微镜(scanning electron microscope,SEM)、X-射线衍射仪(X-ray diffraction,XRD)和电子万能试验机对HPAMAM/GO复合膜的结构和力学性能进行了表征。结果表明:HPAMAM/GO复合膜呈现出类似于贝壳的"砖"和"泥浆"排列的层状结构;随HPAMAM在复合膜中质量分数的提高,复合膜的延展性不断提高;在交联后,G-HPAMAM/GO复合膜的力学性能显著提高,拉伸强度约为150 MPa;复合膜保持了很高的韧性,其断裂伸长率高达10%以上。因此HPAMAM在构筑高强高韧仿贝壳复合材料方面具有非常高的研究价值。展开更多
One of the main challenges of biosensor design is to understand the protein or peptide stability on the chip in high resolution structural detail. Since conventional experimental methods are limited by the resolution ...One of the main challenges of biosensor design is to understand the protein or peptide stability on the chip in high resolution structural detail. Since conventional experimental methods are limited by the resolution for their applications on surface tethered peptides/proteins, a recently developed coarse grained simulation method is employed to explore the peptide/surface interaction in residue-level resolution. This work shows how the coarse grained model successfully describes peptide-surface interactions by evaluating thermal stability of the peptide cecropin PI in bulk solution and on surfaces by physical adsorption and chemical tethering. The simulation also reproduces observations of peptide orientations on the self-assembled monolayer surface from earlier experimental work. Additionally, using knowledge obtained from the simulations, specific mutations are suggested and the desired structure and pose on the surface is obtained. In summary, this work sheds a light on the reasonable biosensor design that is ~uided by simulations.展开更多
文摘文章采用超支化聚合物聚酰胺胺(HPAMAM)和氧化石墨烯(graghene oxide,GO),通过真空抽滤自组装的方法制备了仿贝壳复合材料,并对其用京尼平进行了适当的交联。采用透射电子显微镜(transmission electron microscope,TEM)对GO进行了表征,采用红外光谱(infrared spectroscopy,IR)、扫描电子显微镜(scanning electron microscope,SEM)、X-射线衍射仪(X-ray diffraction,XRD)和电子万能试验机对HPAMAM/GO复合膜的结构和力学性能进行了表征。结果表明:HPAMAM/GO复合膜呈现出类似于贝壳的"砖"和"泥浆"排列的层状结构;随HPAMAM在复合膜中质量分数的提高,复合膜的延展性不断提高;在交联后,G-HPAMAM/GO复合膜的力学性能显著提高,拉伸强度约为150 MPa;复合膜保持了很高的韧性,其断裂伸长率高达10%以上。因此HPAMAM在构筑高强高韧仿贝壳复合材料方面具有非常高的研究价值。
文摘One of the main challenges of biosensor design is to understand the protein or peptide stability on the chip in high resolution structural detail. Since conventional experimental methods are limited by the resolution for their applications on surface tethered peptides/proteins, a recently developed coarse grained simulation method is employed to explore the peptide/surface interaction in residue-level resolution. This work shows how the coarse grained model successfully describes peptide-surface interactions by evaluating thermal stability of the peptide cecropin PI in bulk solution and on surfaces by physical adsorption and chemical tethering. The simulation also reproduces observations of peptide orientations on the self-assembled monolayer surface from earlier experimental work. Additionally, using knowledge obtained from the simulations, specific mutations are suggested and the desired structure and pose on the surface is obtained. In summary, this work sheds a light on the reasonable biosensor design that is ~uided by simulations.
