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Russian Collaborative Development of Reproduction Technologies for the Sustainable Management of Amphibian Biodiversity
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作者 Victor K.UTESHEV Edith N.GAKHOVA +5 位作者 Ludmila I.KRAMAROVA Natalia V.SHISHOVA Svetlana A.KAUROVA Elena A.KIDOVA Artem A.KIDOV Robert K.BROWNE 《Asian Herpetological Research》 SCIE CSCD 2023年第1期103-115,共13页
Reproduction technologies(RTs)can provide for the reliable reproduction of amphibians,as well as perpetuation of species genetic variation with the use of biobanks.In 1982,in anticipation of the biodiversity conservat... Reproduction technologies(RTs)can provide for the reliable reproduction of amphibians,as well as perpetuation of species genetic variation with the use of biobanks.In 1982,in anticipation of the biodiversity conservation crisis,major Russian institutions collaborated in a dynamic program to develop and implement RTs for the sustainable management of amphibian biodiversity.An initial primary focus was the captive breeding of threatened Russian endemic anuran and caudate species,using RTs that varied from environmental manipulation to the use of exogenous gonadotropic hormones to stimulate reproduction.These species were mostly from Palearctic or cool mountain regions,but also included a wide range of species from warm regions.Other early achievements included the successful cryopreservation of anuran spermatozoa and anuran diploid pluripotent cell nuclei,in order to store both the matrilineal and patrilineal genomes in biobanks,with their subsequent development to the blastula stage after implantation into enucleated oocytes.After the turn of the 21st Century,in support of the priorities of the Amphibian Conservation Action Plan(2007),we developed RTs for the refrigerated storage of testicular or urinary spermatozoa for days to weeks at 4℃,the cryopreservation of urinary spermatozoa using anovel cryoprotectant,the in vitro fertilisation of hormonally induced oocytes either fresh or after refrigerated ex situ or in situ storage,and the artificial insemination of salamanders with fresh spermatozoa.In this article,we describe previously unpublished techniques and techniques from obscure Russian sources. 展开更多
关键词 artificial fertilisation assisted reproductive technologies(ART) CRYOPRESERVATION gonadotropic hormones OOCYTES SPERMATOZOA storage urinary spermatozoa
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利血平和丘脑下部促黄体素释放激素类似物(LHRH-A)对大鳞副泥鳅促性腺激素细胞的分泌活动和排卵的促进作用 被引量:2
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作者 林浩然 彭纯 +1 位作者 刘龙志 周溪涓 《动物学报》 SCIE CAS 1985年第4期313-318,390-391,共8页
利血平能使神经分泌细胞的儿茶酚胺贮存量减少甚至消耗殆尽,导致多巴胺作为GRIF的作用减弱,从而显著增强LHRH-A促进GtH分泌和诱导排卵的效应。对大鳞副泥鳅,利血平和LHRH-A同时注射或者利血平比LHRH-A提前3小时注射,都能激活GtH细胞,显... 利血平能使神经分泌细胞的儿茶酚胺贮存量减少甚至消耗殆尽,导致多巴胺作为GRIF的作用减弱,从而显著增强LHRH-A促进GtH分泌和诱导排卵的效应。对大鳞副泥鳅,利血平和LHRH-A同时注射或者利血平比LHRH-A提前3小时注射,都能激活GtH细胞,显著增强其分泌活动并诱导排卵。利血平的最低有效剂量为1—5微克/克体重。利血平(25微克/克体重)+LHRH-A(0.05微克/克体重)的催产效果和多巴胺拮抗物pimozide(1微克/克体重)+LHRH-A(0.05微克/克体重)的催产效果一样,排卵率都达到了100%。 展开更多
关键词 RESERPINE LHEH-A OVULATION GONADOTROPIN Gonadotrops Loach.
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An association study of HFEgene mutation with idiopathic male infertility in the Chinese Han population
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作者 Xiao-Ying Yu Bin-Bin Wang +7 位作者 Zhong-Cheng Xin Tao Liu Ke Ma Jian Jiang Xiang Fang Li-Hua Yu Yi-Feng Peng Xu Ma 《Asian Journal of Andrology》 SCIE CAS CSCD 2012年第4期599-603,共5页
Mutations in the haemochromatosis gene (HFE) influence iron status in the general population of Northern Europe, and excess iron is associated with the impairment of spermatogenesis. The aim of this study is to inve... Mutations in the haemochromatosis gene (HFE) influence iron status in the general population of Northern Europe, and excess iron is associated with the impairment of spermatogenesis. The aim of this study is to investigate the association between three mutations (C282Y, H63D and S65C) in the HFEgene with idiopathic male infertility in the Chinese Han population. Two groups of Chinese men were recruited: 444 infertile men (including 169 with idiopathic azoospermia) and 423 controls with proven fertility. The HFEgene was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The experimental results demonstrated that no C282Y or $65C mutations were detected. Idiopathic male infertility was not significantly associated with heterozygous H63D mutation (odds ratio=O.801, 95% confidence interval=0.452-1.421, X2=0.577, P=0.448). The H63D mutation frequency did not correlate significantly with the serum luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone (T) levels in infertile men (P=0.896, P=0.404 and P=O.05, respectively). Our data suggest that the HFEH63D mutation is not associated with idiopathic male reproductive dysfunction. 展开更多
关键词 gene mutation gonadotropic hormone HFE male infertility
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A Qualitative Model of the Interaction of Sexual Behavior and Hormone Gene Transcription in Male Blue Gourami during Reproduction
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作者 Gad Degani 《Journal of Biophysical Chemistry》 2020年第4期43-50,共8页
In the present study, a model is suggested to describe hormone control in male blue gourami (<i><span style="font-family:Verdana;">Trichogaster trichopterus</span></i><span style=&... In the present study, a model is suggested to describe hormone control in male blue gourami (<i><span style="font-family:Verdana;">Trichogaster trichopterus</span></i><span style="font-family:Verdana;">) along the gonadotropic brain</span><span style="font-size:10pt;font-family:Verdana;">-</span><span style="font-size:10pt;font-family:Verdana;">pituitary</span><span style="font-size:10pt;font-family:Verdana;">- </span><span style="font-size:10pt;font-family:Verdana;">gonad axis (BPG axis) and the hypothalamic-pituitary-somatotropic axis (HPS axis). This model is based on the cloning</span><span style="font-size:10.0pt;font-family:""><span style="font-family:Verdana;"> and transcription of genes encoding hormones of the two axes involved in spermatogenesis during blue gourami reproduction. Gene transcription is affected by environmental, biological, </span><span style="font-family:Verdana;">and behavioral factors. Mature males were examined in two different stages—nonreproductive in high-density habitats and reproductive in low-density </span><span style="font-family:Verdana;">habitats. Based on gene transcription, gonadotropin-releasing hormone 1 (GnRH1) was involved in controlling spermatogenesis (spermatogonia to spermatids) via the BPG axis in nonreproductive and reproductive stages by controlling follicle-stimulating hormone (FSH), 11-ketotestosterone (11KT) and 17</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-estradiol (E</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">). However, GnRH3 had a larger effect during the reproductive stage via the BPG axis (spermatids to sperm) on luteinizing hormone (LH), 11KT, and 17</span><i><span style="font-family:Verdana;">α</span></i></span><span style="font-size:10pt;font-family:Verdana;">-</span><span style="font-size:10pt;font-family:Verdana;">hydroxyprogesterone (17P). At the same time, the HPS axis was involved in spermatogenesis via pituitary adenylate cyclase-activating polypeptide (PACAP) and its related peptide PRP (formerly known as GHRH-like peptide) in the brain, and growth hormone (GH) in the pituitary affected synthesis of insulin-like growth factor 1 (IGF1) in the liver.</span> 展开更多
关键词 Qualitative Model Anabantidae HORMONE Gene Trichogaster SPERMATOGENESIS SPERM GNRH 11-Ketotestosterone gonadotropic Brain Pituitary Gonad Axis
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ULTRASTRUCTURAL STUDY OF THE HATSCHEK'S PIT EPITHELIAL CELLS OF AMPHIOXUS 被引量:2
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作者 方永强 齐襄 《Science China Chemistry》 SCIE EI CAS 1989年第12期1465-1472,共8页
The authors have observed, using an electron microscope, that the number of thesecretory granules and the development of various organelles in the apical epithelial cellof the Hatschek’s pit showed a positive correla... The authors have observed, using an electron microscope, that the number of thesecretory granules and the development of various organelles in the apical epithelial cellof the Hatschek’s pit showed a positive correlation with the gonadal development andmaturity. At the same time, only the epithelial cells respond to GnRH-A(gonadotropin-releasing hormone analogue). Both cytological and endocrinological experiments demon-strate that the cell may be the primitive gonadotropic cell of amphioxus. 展开更多
关键词 AMPHIOXUS Hatschek’s PIT EPITHELIAL CELL ULTRASTRUCTURE gonadotropic CELL
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