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The assessment of good and bad bacteria in holy basil(Ocimum sanctum)leaves
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作者 Bhoj R Singh Mudit Chandra +3 位作者 Himani Agri Ravichandran Karthikeyan Akanksha Yadav Varsha Jayakumar 《Infectious Diseases Research》 2023年第4期1-21,共21页
Holy basil(Tulsi,Ocimum sanctum)is considered to be a pious plant.It is used not only in many of the rituals of Hindus but is also known for its innumerable medicinal qualities.Holy basil leaves(HBLs)are often consume... Holy basil(Tulsi,Ocimum sanctum)is considered to be a pious plant.It is used not only in many of the rituals of Hindus but is also known for its innumerable medicinal qualities.Holy basil leaves(HBLs)are often consumed raw thus it is pertinent to detect the bacteria on Tulsi leaves.This study was conducted to understand the diversity of the aerobically growing microbiome of edible holy basil leaves.A total of 112 samples of leaves were collected from households of six localities in and around Bareilly,India for detecting culturable bacteria growing aerobically.A sum of 579 bacterial isolates belonging to 106 different species was identified.Pantoea agglomerans isolates were detected in 43 samples followed by isolates Virgibacillus pantothenticus(21),Bacillus coagulans(18),Bacillus cereus(17),Geobacillus stearothermophilus(16),Klebsiella pneumoniae(13),Citrobacter freundii(12),Lysinibacillus sphaericus(12),Acinetobacter calcoaceticus(11),Escherichia coli(11),and Xenorhabdus bovienii(11).Isolates of 95 species of bacteria were detected on<10 samples of HBLs.Of 579 isolates,161 isolates detected in 68(60.71%)samples were of putatively good bacteria belonging to eight genera(Bacillus,Brevibacillus,Lysinibacillus,Paenibacillus,Photobacter,Siccibacter,Virgibacillus,Xenoirhabdus)and 23 species.However,418 bacterial isolates detected in 108(96.43%)of the HBL samples were classified as potentially pathogenic for humans and animals belonging to 34 genera and 73 species.There were only four samples(three from Mahanagar and one from Bhojipura)that had no potential pathogenic bacteria detected in this study.The analysis indicated that HBL leaves from the ICAR-IVRI campus had a lower probability(P<0.04)of harbouring good bacteria than samples from Bhojpura,Mahanagar,North City and Rajendra Nagar.Cinnamaldehyde inhibiting 96.72%of the isolates and carvacrol inhibiting 96.37%of the isolates were the best herbal antimicrobials and among antibiotics,meropenem inhibited 98.96%of bacterial isolates.Antimicrobial resistance in non-pathogenic or good bacteria was significantly more common(P<0.01)for ajowan oil but they were more often(P<0.05)susceptible to holy basil oil,lemongrass oil,citral,P.embalica leaf extract,P.longifolia seed oil,nitrofurantoin,ciprofloxacin,chloramphenicol,colistin and minocycline than isolates of potentially pathogenic bacteria.The study concludes that HBLs may harbour both good and bacteria,and should be consumed raw only after proper decontamination. 展开更多
关键词 Ocimum sanctum antimicrobial susceptibility assay aerobic culture isolation good and bad bacteria
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历届奥运会举办地的选择及利弊分析 被引量:1
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作者 张玲玲 《山西师大体育学院学报》 2000年第3期1-4,共4页
奥运会是目前世界上规模最大、水平最高、影响最广的国际性综合运动会,其宗旨是为建立一个和平、美好的新世界做出贡献。从1896年现代奥运会诞生到现在,一百多年来奥运会已经举办了26届。分析历届奥运会举办地的选择方式,研究其利弊,对... 奥运会是目前世界上规模最大、水平最高、影响最广的国际性综合运动会,其宗旨是为建立一个和平、美好的新世界做出贡献。从1896年现代奥运会诞生到现在,一百多年来奥运会已经举办了26届。分析历届奥运会举办地的选择方式,研究其利弊,对改革和发展奥运会大有稗益。 展开更多
关键词 the olympic games PLACE analyse good and bad
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Good News and Bad News
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作者 小非 柴立青 《中学生天地(初中综合版)(A版)》 2004年第12期36-36,共1页
关键词 bad good News and bad News
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