Comparative Study of Bovine Pericardium and Gore-Tex in Tissual Interaction with Wistar Rats Diaphragm

Introduction: Simple suture isn’t always possible in large congenital diaphragmatic hernia (cdh) defects. Synthetic materials are used for correction such as Silastic&reg, Gore-Tex&reg (GT), Teflon&reg or biological, such as autologous muscle patches. It was shown that bovine pericardium (bp) was effective to correct those large defects with many positive outcomes when compared with syntactic materials. Aim: This study aims to establish an experimental model of correction for large diaphragmatic defect with PB and GT patches to compare histologically the tissue interaction between them and diaphragm in young Wistar rats. Materials & Methods: 15 wistar rats were divided in 3 groups: Rats that used BP was named G1;Rats that used GT was named G2;and rats with only scraping in the diphragm, named G3 (control). Animals were submited to a laparotomy and fixed pathces to diaphragms and harvested 3 weeks later. Area between normal diaphragm and patches were isolated and separated for histological analysis, such as lymphocytic infiltration (inflammation), neovascularization and fibrosis. Results: G1 presented inflammation between BP and Diaphragm In 5 Samples. G2 Presented Neovascularization In 5 Samples, But No inflammation. Fibrotic tissue overlapping GT patches occurred in 3 samples in G2. Comparing G1 with G2 there was a significant statistical difference concerning inflammation (P = 0.0079), in G1. Comparing neovascularization there is no significant statistical difference (P = 0.4444), despite a slight higher incidence in G2. Fibrosis in both groups presented no significant statistical difference (P = 0.4444), despite a slight higher incidence in G2. There were no alterations in G3. Discussion: Despite the statistical difference in the inflammatory process was more frequent in G1 (P = 0.0079), neovascularization and fibrosis were more frequent in G2. Conclusion: The proposed experimental model was satisfactory to reproduce suture of patches in the diaphragm. These results suggests that inflammation, neovascularization and fibrosis indeed contribute to a benign healing process that reacts differently in each group but can drive to a more lasting and permanent results when biological patch is considered. Statistical report suggests that this study should be continued with a larger sample of animals and a wider period of time before harvest.

Outcomes of surgical correction of Peyronie’s disease with plaque excision and grafting: Comparison of testicular tunica vaginalis graft versus bovine pericardium graft

Objective:Peyronie’s disease(PD)is an abnormal wound healing in the penile tunica albuginea.After fibrotic plaque excision,different graft materials have been used to repair the defects,but the optimal graft remains unknown.This study aimed to compare the functional outcomes of testicular tunica vaginalis grafts and bovine pericardium grafts in patients with severe PD.Methods:A retrospective comparative study was conducted on 33 PD patients undergoing partial plaque excision and grafting from September 2015 to May 2021.The patients were divided into two groups depending on the type of graft used.For 15 patients in Group B,testicular tunica vaginalis grafts were used to repair the defect,while for 18 patients in Group A,bovine pericardium grafts were used.Data of the patient’s age,comorbidities,sexual function,penile curvature,postoperative complications,need for further treatment,change in penile length,and satisfaction were gathered and compared between the groups.Sexual function was evaluated using the 5-item version of the International Index of Erectile Function(IIEF-5),and a functional less than 20-degree penile curvature after surgery was considered a successful intervention.Results:There was no difference in age,comorbidities,degree of curvature,perioperative IIEF-5,operative time,plaque size,or complication rates.After surgery,a statistically significant improvement in curvature degree(p<0.05)and satisfactory penile appearance(p<0.05)were seen in both groups without any superiority between the two groups(p=0.423 and p=0.840,respectively).With a 30-month follow-up,the IIEF-5 was consistent in both groups,with no statistical significance between the groups(p=0.492).The main change in penile length during the operation was increased and still positive in the last follow-up in both groups without statistical significance(p=0.255 and p=0.101,respectively).Conclusion:Partial plaque excision and corporoplasty with both testicular tunica vaginalis or bovine pericardium grafts are equally effective in treating males with clinically significant PD.

心瓣膜Gore-Tex人工腱索植入前后超微形态研究

目的:观察Gore Tex线的超微形态和结构。方法:采用CV 4Gore Tex线,在植入前及植入绵羊、病人体内(二尖瓣腱索替换)后,分别于71及626天进行扫描、透射电镜观察。结果:本材料由呈纵行排列的纤细纤维构成,线内有许多气泡样间隙。植入绵羊体内71天后,线表面有宿主内皮细胞层覆盖生长。植入人体626天,表面有少数内皮细胞覆盖,表层下有成纤维细胞长入。结论:Gore Tex线具有微孔结构,利于宿主内皮细胞覆盖及成纤维细胞长入,是人工腱索替换的适合材料。

Gore-tex防水透湿层压织物的概述

介绍了各类防水透湿织物的种类、性能及研究发展状况,着重叙述了商品名为Gore-tex的PTFE微孔膜层压织物,并提出了存在的问题.

Gore-Tex多线圈技术在心脏瓣膜腱索重建中的应用

目的评价使用Gore-Tex线圈技术在心脏瓣膜重建术中的疗效。方法比较11例使用Gore-Tex线圈技术治疗二尖瓣脱垂患者术前、术后心功能和二尖瓣反流程度的变化。结果术后心功能较术前明显改善,射血分数(EF)从术前的(0.52±0.03)提高至(0.62±0.07)(P<0.05)。术中超声检查无明显二尖瓣反流7例,二尖瓣轻微反流2例,轻度反流2例;术后1月均常规复查心脏彩超,二尖瓣无明显反流6例,轻微反流3例,轻度反流2例。结论 Gore-Tex线圈技术应用于心脏瓣膜腱索重建疗效满意,与传统腱索重建技术相比较更方便、安全。

Gore-Tex缝线四点式人工晶状体睫状沟固定术的临床疗效

目的评价Gore-Tex缝线四点式人工晶状体睫状沟固定术的临床疗效和安全性。方法回顾性分析行人工晶状体睫状沟缝线固定术的患者29例(30眼),其中20例(20眼)采用传统聚丙烯缝线人工晶状体睫状沟缝线固定术(对照组),9例(10眼)行Gore-Tex缝线四点式人工晶状体睫状沟缝线固定术(四点式组)。对术后裸眼视力、验光值、裂隙灯检查及眼压结果等进行统计分析,随访时间为12个月。结果四点式组于术后1个月开始裸眼视力明显提高(P<0.05),对照组于术后3个月开始裸眼视力明显提高(P<0.05)。术后1、3、6、12个月时,四点式组较术前裸眼视力提高量均优于对照组(P<0.05)。术后1周和1、3、6、12个月时,四点式组眼内残余散光值均小于对照组(P<0.05)。2组均未出现严重的眼部并发症。结论Gore-Tex缝线四点式人工晶状体睫状沟固定术通过四点固定,使术后人工晶状体不易发生旋转和偏斜,显著降低术后眼内残余散光,更早改善术后裸眼视力,手术安全有效。

应用Gore-Tex人造血管重建颈总动脉的实验研究

目的 观察人造血管移植后不同时间的通畅率和组织病理学变化 ,探讨人造血管移植失败的原因。方法 3只健康成年山羊作为实验对象 ,手术切除 2 cm颈总动脉 ,以口径 4m m的 Gore- Tex人造血管进行桥接。 3只动物的 6侧颈部随机分为 A、B两组 ,各含 3条动脉。A组的近、远心端均为端 -端吻合术 ,B组的近心端为端 -侧吻合术 ,远心端为端 -端吻合术。移植术后 1周 ,以激光多普勒血流仪监测移植血管通畅情况 ,并择例行颈动脉造影术。术后 2周、3月、6月分期切取标本 ,观察人造血管不同时间的通畅率。标本经处理后分别作光镜、透射电镜和扫描电镜检查。结果 A、B两组人造血管于术后 2周、3月和 6月时的通畅情况分别为 1/1,0 /1,0 /1和 0 /1,1/1,1/1,其差别无显著意义 (χ2 =0 .1414,P=1.0 )。组织病理学检查显示 ,植入后 2周 ,人造血管内壁有少量内皮细胞覆盖 ,结缔组织外膜形成。植入后 3月 ,吻合口处动脉内膜增生 ,向人造血管中段移行 ,延伸长度距吻合口约 6 mm。人造血管新内膜形成不均一 ,部分区域出现过度增生。吻合后 6月 ,扫描电镜显示内皮细胞覆盖不完全 ,延伸长度距吻合口8m m。结论 以人造血管修复颈动脉缺损是可行的 ,但自然内皮化相对有限 ,而且新内膜形成不均。血栓形成和新内膜过度增生 ,均?

Gore-Tex面料透湿性能对比测试

采用YG 601型织物透湿仪和被动式微气候仪,在3种标准试验条件和4种微气候仪条件下,对3种6块Gore-Tex试样和2块棉织物试样进行了透湿性能测试。在标准透湿杯条件下,各试样的透湿量差异相对较大,平均透湿量与透湿量差异与试样两侧所存在的蒸汽压差成正比,Gore-Tex试样平均透湿能力要小于普通棉织物。采用被动式微气候仪的透湿试验发现:试样的透湿量与模拟皮肤内的水温成正比,水温越高,皮肤出汗量越大,内外湿度差越大,试样的透湿量也越大,同时微气候室内的积水也越多;在稳态情况下,微气候室内的积水量随时间成正比;在相同条件下Gore-Tex试样及棉织物试样的透湿量相差很小,但前者在微气候室内的积水量要大一些,也说明不同Gore-Tex织物的透湿量接近但还是小于普通棉织物。虽然Gore-Tex织物的透湿量接近棉织物,但其透气性能要远低于普通织物。所以,Gore-Tex织物称作防风透湿织物是准确的,其主要性能取决于PTFE(聚四氟乙烯)微孔膜的性能。

Gore-Tex在耳郭部分缺损修复中的应用

目的:探索用于部分耳郭缺损再造的理想材料。方法:对13例外伤后导致的部分耳郭缺损的病人,采用Gore-Tex整形材料作为再造耳的支架,在耳后设计局部皮瓣覆盖进行修复,供瓣区直接缝合或另取皮片植皮。结果:本组共13例13耳,4例术后发生假体外露,经再次手术修整,随访1～2年,所有再造耳郭外形满意,与对侧正常耳外形基本一致。结论:Gore-Tex材料具有良好的组织相容性和可塑性,是部分耳郭再造的理想材料之一。

1例胸腔镜下Gore-Tex线圈腱索重建术的护理配合

[目的]总结1例全胸腔镜下Gore-Tex线圈腱索重建术的护理配合。[方法]回顾性分析1例全胸腔镜下Gore-Tex线圈腱索重建病例的手术过程。[结果]手术过程顺利,病人3个月后及6个月后分别进行心脏彩超复查,均报告二尖瓣关闭良好,未见反流。[结论]充分的术前准备工作、积极默契的医护配合、落实到位的安全护理是胸腔镜下Gore-Tex线圈腱索重建术顺利完成的保证。

Mechanisms on Correlation between the Pericardium Meridian and Heart Effects of Electroacupuncture at Neiguan and Other Acupo

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Does the Animal Origin Influence the Calcification of Xenograft Tissue Heart Valve Substitutes? Comparison between Bovine and Camel Pericardium in a Subcutaneous Rat Model

Objective: To validate the hypothesis that camel pericardium could be more protected than bovine pericardium against calcification process according to the huge difference in their respective lifestyle and lifetime. Methods: Glutaraldehyde (GA) fixed bovine and camel pericardium samples (BP and CP respectively) were both implanted in 30 New Zealand white rats (2 BP and 2 CP matched specimens in each animal) and explanted after 60 days. Unimplanted GA-fixed samples of both species served as control. Matched implanted samples and unimplanted samples were randomly submitted to elemental analysis by spectroscopy, phospholipid extraction, macroscopic and X-ray examination and histology. Results: At 60 days, calcium and phosphorus content were respectively 9.54% ± 3.1% and 4.79% ± 1.4% of tissue dry weight in BP, and 12.52% ± 2.7% and 6.14% ± 1.3% of tissue dry weight in CP (ns). In X-ray analysis, the calcification score was 1.28 ± 0.45 and 2.14 ± 0.98 in BP and CP samples respectively without significant difference (p < 0.08). In histology, calcifications were lower in BP than in CP: 1.37 ± 0.85 vs 2.28 ± 0.83 (ns);collagen fibers were better conserved in BP than in CP: 2.4 ± 0.48 vs 1.87 ± 0.78 (ns), and less disoriented: 25% vs 62% (ns). In unimplanted samples, there was a higher but not significant rate of extracted lipids in CP: 5.7 ± 1.8 vs 9.5 ± 3.8 nanomoles in PS fraction and 11.3 ± 3.7 vs 19 ± 7.7 nanomoles in total fatty acids, in BP and CP samples respectively. All results were in conjunction and demonstrated a higher but not significant rate of mineralization in camel pericardium after implantation, which could be related to a higher but not significant basic rate of phospholipid and fatty acids. Conclusion: This experiment study in a subcutaneous rat model has failed to valid our hypothesis. Because the differences observed between bovine and camel pericardium did not reach the significance, at the best, there is no difference between both species and at the worst, camel pericardium has a higher rate of the phosphatidylserine fraction of phospholipid, and is more sensitive and prompt to calcification.

Gore-Tex人造血管动脉搭桥术治疗股腘动脉硬化闭塞症23例疗效分析

采用膨体聚四氟乙烯人造血管（Gore－Tex人造血管）作膝上段股动脉搭桥术治疗下肢股动脉粥样硬化闭塞症23例．经术后1月～10年的随访．有效率达78．3％，肢体存活率达90.4％。此手术操作简单，在缺乏合适的大隐静脉时，采用聚四氟乙烯人造血管作膝上段动脉代用品，其疗效非常满意。

Gore-Tex膜引导骨再生技术在种植体周围炎治疗中的应用

目的 :评价Gore_Tex膜引导骨再生技术治疗种植体周围炎的临床疗效。方法 :对3例种植体周围炎患者局部冲冼和药物治疗控制感染后行翻瓣刮治 ,应用Gore_Tex膜覆盖骨缺损 ,术后6月去除膜 ,摄X线片及进行临床评价。结果 :3例骨缺损者均完全再生修复 ,种植体稳定。结论 :局部冲冼和翻瓣刮治后 ,应用Gore_Tex膜覆盖骨缺损治疗种植体周围炎 ,可获得骨组织再生修复。

Use of bovine pericardium graft for abdominal wall reconstruction in contaminated fields

AIM:To employ, in such conditions, a biological graft such as bovine pericardium that offers resistance to infection. METHODS:In our surgical department, from January 2006 to June 2010, 48 patients underwent abdominal wall reconstruction using acellular bovine pericardium; of these 34 patients had a contaminated wound due to diffuse peritonitis (complicated diverticulitis, bowel perforation, intestinal infarction, strangled hernia, etc. ) and 14 patients had hernia relapse on infected synthetic mesh. RESULTS: In our series, one patient died of multiorgan failure 3 d after surgery. After placement of the pericardium mesh four cases of hernia relapse occurred. CONCLUSION: Recurrence rate is similar to that of prosthetic mesh repair and the application of acellular bovine pericardium (Tutomesh , Tutogen Medical Gmbh Germany) is moreover a safe and feasible option thatcan be employed to manage complicated abdominal wall defects where prosthetic mesh is unsuitable.

Valve Repair Using Autologous Pericardium in Tricuspid Valve Endocarditis

Tricuspid valve endocarditis is a significant cause of valve-related morbidity and mortality. Prosthetic valve reconstruction can be costly and requires life-long anticoagulation. We present our experience with surgical management of an endocarditis damaged tricuspid valve leaflet in an intravenous drug user. We were able to excise the infected tissue and successfully repair the native valve using autologous pericardium, effectively eliminating the need for prostheses and associated complications. Our case report demonstrates that tricuspid valve reconstruction, using autologous pericar-dium, is a suitable option in patients with tricuspid endocarditis.

Primary synovial sarcoma of pericardium: A case report

Primary pericardial sarcomas are extremely rare. We report a case of 19 year old male who presented with cough, dyspnoea, and orthopnea. Investigations and exploratory thoracotomy revealed a large pericardial mass. Surgical debulking of the tumor was performed and the histopathological examination was compatible with synovial sarcoma. The tumor was unresectable due to its invasion and adhesion to mediastinal structures. Hence patient was started on palliative chemotherapy (adriamycin and ifosfamide based). Patient showed an initial symptomatic response but later on there was a clinical progres-sion and died within six months of his diagnosis.

Aortic Neocuspidization with Autologous Pericardium: Initial Experience of Single Center

Background: Aortic neo-cuspidization (AVNeo) procedure has been adopted by limited centers with the publication of the mid-long term successful results. The aim of this study was to present initial experience of the AVNeo procedure of a single center. Methods: The medical records of 24 patients who underwent AVNeo with or without concomitant cardiac surgery between February 2019 and February 2021 at our tertiary hospital were scanned retrospectively. Results: The mean age of patients was aged 58.21 ± 13.14 years and 16 (66.7%) of them were men. 16 patients were operated on for aortic stenosis (66.67%). Morphology of the aortic valve was tricuspid in 21 (87.5%) and bicuspid in 3 (12.5%) of the patients. Additional cardicac surgery was performed in 13 (54.17%) patients. No patients needed reoperation for bleeding, pacemaker implantations, conversion to classical prosthetic aortic valve replacement, or infective endocarditis. Two patients died due to non-cardiac reasons. Preoperative peak and mean aortic valve pressures improved significantly at 1st and 6th months (Preop: 89.06 ± 21.88 mmHg and 56.38 ± 15.09 mmHg 1st month: 22.00 ± 3.93 mmHg and 8.73 ± 2.60 mmHg, 6th month: 18.13 ± 3.02 mmHg and 6.93 ± 1.83 mmHg). Conclusion: In conclusion, the AVNeo procedure is a feasible technique for aortic valve pathologies, with the advantages of avoiding anticoagulants and the applicability of concomitant surgical procedures. Although this procedure requires meticulous experience, results similar to the available published literature can be obtained and reproducible even during learning curve when technical steps are strictly followed.

Primary primitive neuroectodermal tumor in the pericardium-a focus on imaging findings:A case report

BACKGROUND Primitive neuroectodermal tumors(PNETs)are rare,sporadic malignant tumors of the peripheral nervous system,bone,or soft tissues.However,to the best of our knowledge,only three cases of PNET in the pericardium have been reported in the English literature,and their magnetic resonance imaging findings have not previously been described.CASE SUMMARY A 3-year-old boy was hospitalized with a 1-wk history of recurrent vomiting and weakness.Detailed history-taking revealed no evidence of heart disease.Computed tomography demonstrated a soft tissue mass in the left pericardial cavity with heterogeneous contrast enhancement.The border between the mass and the heart was poorly defined.Thoracotomy revealed a mass invading the left ventricle,with a high risk of bleeding.The mass was considered inoperable.A biopsy was performed,and the histological and immunohistochemical findings confirmed the diagnosis of primary PNET of the pericardium.The patient received four cycles of standard chemotherapy.Chest magnetic resonance imaging 3 mo after the initiation of chemotherapy revealed that the tumor in the pericardium still existed,but its volume had slightly decreased.The patient was lost to follow-up,and the final outcome was therefore unknown.CONCLUSION Medical imaging plays an important role in defining the pericardial origin of PNET and understanding its characteristics.Magnetic resonance imaging can provide more information on the tumor than computed tomography and may thus aid therapeutic planning.