Stepwise selection approach was adopted to obtain glyphosate-tolerant upland cotton mutant(R1098) from the embryogenic calli of Coker 312(Gossypium hirsutum L.).The calli were transferred to selection medium and multi...Stepwise selection approach was adopted to obtain glyphosate-tolerant upland cotton mutant(R1098) from the embryogenic calli of Coker 312(Gossypium hirsutum L.).The calli were transferred to selection medium and multi-step selection pressure process was carried out until the展开更多
Actin cytoskeleton plays an important role in cell morphogenesis in plants as demonstrated by pharmacological,biochemical,and genetic studies.The actin cytoskeleton may be involved in
CAP,an adenylyl cyclase-associated protein,is predicted to be involved in cytoskeletal organization and signal transduction.Recently,we found that CAP may play an important role in fuzz-like fiber cell initiation in c...CAP,an adenylyl cyclase-associated protein,is predicted to be involved in cytoskeletal organization and signal transduction.Recently,we found that CAP may play an important role in fuzz-like fiber cell initiation in cotton.For the further research,we isolated two CAP homologues from wild展开更多
Mutations are possibly the only source ofcreating heritable variability in all biologicalsystem and,many useful mutants in plants havebeen released for commercial cultivation acrossthe world.To-date throughout the wor...Mutations are possibly the only source ofcreating heritable variability in all biologicalsystem and,many useful mutants in plants havebeen released for commercial cultivation acrossthe world.To-date throughout the world about2252 mutants have been officially registered inthe FAO/IAEA mutant varieties data展开更多
Glandless or gossypol free cottons are of greatvalue to develop)the utilizations of cottonseedsand cotton scientists have been interested indeveloping more applicable glandless cultivars.Common glandless cotton are ge...Glandless or gossypol free cottons are of greatvalue to develop)the utilizations of cottonseedsand cotton scientists have been interested indeveloping more applicable glandless cultivars.Common glandless cotton are generated byrecessive genes,such as gl<sub>2</sub> and gl<sub>3</sub>.A dominantglandless mutant has been discovered展开更多
A fuzzless-lintless (fl) seed mutant of Gossypium hirsutum L. cv. Xu-142 was investigated to study cotton fiber development. Scanning electron microscopy revealed that fiber initials were virtually absent from fl ovul...A fuzzless-lintless (fl) seed mutant of Gossypium hirsutum L. cv. Xu-142 was investigated to study cotton fiber development. Scanning electron microscopy revealed that fiber initials were virtually absent from fl ovules. RT-PCR analysis showed that the steady-state levels of transcripts of the fiber-specific E6 and Expansin genes were high in wild type (WT) ovules during the fiber initiation and elongation stages, and peaked around 15 days post anthesis (DPA), but only a trace amount of these transcripts was detectable in fl ovules of alt developmental stages investigated. CotmybA, a member of the Myb family, exhibited a clear expression in developing WT ovules, but the expression was abnormal in fl ovules. Application of GA3, or GA3 plus IAA, to the culture medium rescued in vitro fiber initiation and growth of fl ovules only partially. In addition, transcription of E6 and Expansin genes of in vitro cultured WT and fl ovules responded similarly to exogenous hormones. The hormones had less effect on CotmybA transcription in ovules cultured in vitro, and again the WT and fl ovules showed a similar expression. These results suggest that the abnormal or extremely low level of expression of E6, Expansin and CotmybA genes in developing fl seeds is related to the absence of seed-hairs, and the mechanism underlying needs further investigation.展开更多
A series of fiber-specific mutants, or germplasms, have been recently used in the study of fiber development. In the current study, scanning electron microscopy （SEM） was used to investigate developmental difference...A series of fiber-specific mutants, or germplasms, have been recently used in the study of fiber development. In the current study, scanning electron microscopy （SEM） was used to investigate developmental differences in lint and fuzz initiation in different genotypes （Gossypium hirsutum） of upland cotton. These fiber mutants included dominant naked seed N1, recessive naked seed n2, Xuzhou-142 lintless-fuzzless （XZ142WX）, Xinxiangxiaojilintless-fuzzless （XinWX）, Xinxiangxiaojilinted-fuzzless （XinFLM）, with TM-1, the cytogenetic and genetic experimental standard stock, as the control. Characteristics of fiber initiation were analyzed from -1 to ＋1 days post anthesis （dpa） and at 4 and 5 dpa for fuzz initiation. Our data suggested that lint initiation centered on day of anthesis （0dpa）, and elongated significantly at 1dpa, while fuzz initiation began at 4dpa, although the shape of fuzz protrusions differed from that of lint fibers. Fiber initiation occurred first on the ovule funicular crest. Compared to TM-1, there was a noted retardation in development and fiber protrusion in N1 and XinFLM. Microscopy data also demonstrated that lintless-fuzzless mutants （XZ142WX and XinWX） developed irregular protrusions during early developmental stages, which were unable to grow into fiber.展开更多
A fiberless seed mutant(fl)was identified in acommercial cotton(Gossypium hirsutum L.)variety Xu-Zhou 142(FL).This phenotype isassociated with lack of fiber cell initiation in theouter integument of the ovule,as wasch...A fiberless seed mutant(fl)was identified in acommercial cotton(Gossypium hirsutum L.)variety Xu-Zhou 142(FL).This phenotype isassociated with lack of fiber cell initiation in theouter integument of the ovule,as wascharacterized by analysis of genes related to展开更多
The NSF Cotton Genome Centers EST projecthas released】36000 cotton fiber EST sequencesfrom Gossypium arboreum,an A-genome diploidspecies.Of the approximately 10000 genesexpressed in rapidly elongating cotton fibers,5...The NSF Cotton Genome Centers EST projecthas released】36000 cotton fiber EST sequencesfrom Gossypium arboreum,an A-genome diploidspecies.Of the approximately 10000 genesexpressed in rapidly elongating cotton fibers,50% or more encode unknown gene functions.The next challenge facing cotton researchers isdetermining the function of the fiber genes,andwhat role each plays in determiningagronomically important fiber traits.展开更多
文摘Stepwise selection approach was adopted to obtain glyphosate-tolerant upland cotton mutant(R1098) from the embryogenic calli of Coker 312(Gossypium hirsutum L.).The calli were transferred to selection medium and multi-step selection pressure process was carried out until the
文摘Actin cytoskeleton plays an important role in cell morphogenesis in plants as demonstrated by pharmacological,biochemical,and genetic studies.The actin cytoskeleton may be involved in
文摘CAP,an adenylyl cyclase-associated protein,is predicted to be involved in cytoskeletal organization and signal transduction.Recently,we found that CAP may play an important role in fuzz-like fiber cell initiation in cotton.For the further research,we isolated two CAP homologues from wild
文摘Mutations are possibly the only source ofcreating heritable variability in all biologicalsystem and,many useful mutants in plants havebeen released for commercial cultivation acrossthe world.To-date throughout the world about2252 mutants have been officially registered inthe FAO/IAEA mutant varieties data
文摘Glandless or gossypol free cottons are of greatvalue to develop)the utilizations of cottonseedsand cotton scientists have been interested indeveloping more applicable glandless cultivars.Common glandless cotton are generated byrecessive genes,such as gl<sub>2</sub> and gl<sub>3</sub>.A dominantglandless mutant has been discovered
文摘A fuzzless-lintless (fl) seed mutant of Gossypium hirsutum L. cv. Xu-142 was investigated to study cotton fiber development. Scanning electron microscopy revealed that fiber initials were virtually absent from fl ovules. RT-PCR analysis showed that the steady-state levels of transcripts of the fiber-specific E6 and Expansin genes were high in wild type (WT) ovules during the fiber initiation and elongation stages, and peaked around 15 days post anthesis (DPA), but only a trace amount of these transcripts was detectable in fl ovules of alt developmental stages investigated. CotmybA, a member of the Myb family, exhibited a clear expression in developing WT ovules, but the expression was abnormal in fl ovules. Application of GA3, or GA3 plus IAA, to the culture medium rescued in vitro fiber initiation and growth of fl ovules only partially. In addition, transcription of E6 and Expansin genes of in vitro cultured WT and fl ovules responded similarly to exogenous hormones. The hormones had less effect on CotmybA transcription in ovules cultured in vitro, and again the WT and fl ovules showed a similar expression. These results suggest that the abnormal or extremely low level of expression of E6, Expansin and CotmybA genes in developing fl seeds is related to the absence of seed-hairs, and the mechanism underlying needs further investigation.
基金Supported by the National Natural Science Foundation in China （30270806, 30471104）, the State Key Basic Research and Development Plan of China （2002CB111303）, the Program for New Century Excellent Talents in University （NCET-04-0500）, and the Program for Changjiang Scholars and Innovative Research Team in University.We thank the College of Life Sciences in Nanjing Agricultural University for help with SEM analysis.
文摘A series of fiber-specific mutants, or germplasms, have been recently used in the study of fiber development. In the current study, scanning electron microscopy （SEM） was used to investigate developmental differences in lint and fuzz initiation in different genotypes （Gossypium hirsutum） of upland cotton. These fiber mutants included dominant naked seed N1, recessive naked seed n2, Xuzhou-142 lintless-fuzzless （XZ142WX）, Xinxiangxiaojilintless-fuzzless （XinWX）, Xinxiangxiaojilinted-fuzzless （XinFLM）, with TM-1, the cytogenetic and genetic experimental standard stock, as the control. Characteristics of fiber initiation were analyzed from -1 to ＋1 days post anthesis （dpa） and at 4 and 5 dpa for fuzz initiation. Our data suggested that lint initiation centered on day of anthesis （0dpa）, and elongated significantly at 1dpa, while fuzz initiation began at 4dpa, although the shape of fuzz protrusions differed from that of lint fibers. Fiber initiation occurred first on the ovule funicular crest. Compared to TM-1, there was a noted retardation in development and fiber protrusion in N1 and XinFLM. Microscopy data also demonstrated that lintless-fuzzless mutants （XZ142WX and XinWX） developed irregular protrusions during early developmental stages, which were unable to grow into fiber.
文摘A fiberless seed mutant(fl)was identified in acommercial cotton(Gossypium hirsutum L.)variety Xu-Zhou 142(FL).This phenotype isassociated with lack of fiber cell initiation in theouter integument of the ovule,as wascharacterized by analysis of genes related to
文摘The NSF Cotton Genome Centers EST projecthas released】36000 cotton fiber EST sequencesfrom Gossypium arboreum,an A-genome diploidspecies.Of the approximately 10000 genesexpressed in rapidly elongating cotton fibers,50% or more encode unknown gene functions.The next challenge facing cotton researchers isdetermining the function of the fiber genes,andwhat role each plays in determiningagronomically important fiber traits.
基金The authers sre greafiy indebted to Dr.Russell J.KohelUSDA ARS+5 种基金Southern Crops Research LaboratoryCollege StationTX77845USAfor providing TM-1T586 and n2 cotton seeds and giving the instructive help
