目的分析PTGD后择期LC治疗对GradeⅡ急性胆囊炎ACTH、MPO及Cor水平的影响。方法选取2021年12月至2023年5月安徽中科庚玖医院收治的急性胆囊炎患者121例,根据治疗方案分为三组,即甲组(急诊行LC治疗,未行PTGD)38例、乙组(PTGD引流管拔出后...目的分析PTGD后择期LC治疗对GradeⅡ急性胆囊炎ACTH、MPO及Cor水平的影响。方法选取2021年12月至2023年5月安徽中科庚玖医院收治的急性胆囊炎患者121例,根据治疗方案分为三组,即甲组(急诊行LC治疗,未行PTGD)38例、乙组(PTGD引流管拔出后72 h后行LC,早期)43例和丙组(PTGD引流管拔出后14~30 d后行LC,晚期)40例。对比三组手术情况、炎症因子、肝功能、ACTH、MP、Cor水平及并发症发生率。结果甲组LC手术时长、术后卧床时长及住院天数均长于丙组、乙组,失血量、中转开腹率高于丙组、乙组,差异有统计学意义(P<0.05);丙组LC手术时长、术后卧床时长及住院天数均长于乙组,差异具有统计学意义(P<0.05)。LC术后1 d hs-CRP、PCT、IL-6、ST、ALT、ALP、ACTH、MPO及Cor:甲组>丙组>乙组,差异有统计学意义(P<0.05)。并发症发生率:甲组>丙组>乙组,差异有统计学意义(P<0.05)。结论PTGD后择期LC治疗对GradeⅡ急性胆囊炎ACTH、MPO及Cor水平影响小,且并发症低;而PTGD后早期行LC能有效改善肝功能、炎症因子,且术后应激反应更低,值得临床推广。展开更多
目的探讨经皮胆囊穿刺引流(PTGD)后择期腹腔镜胆囊切除术治疗GradeⅡ急性结石性胆囊炎患者的效果。方法回顾性选取2017年8月至2020年6月尉氏县中心医院收治的52例行急诊腹腔镜胆囊切除术GradeⅡ急性结石性胆囊炎患者(对照组)及52例行PTG...目的探讨经皮胆囊穿刺引流(PTGD)后择期腹腔镜胆囊切除术治疗GradeⅡ急性结石性胆囊炎患者的效果。方法回顾性选取2017年8月至2020年6月尉氏县中心医院收治的52例行急诊腹腔镜胆囊切除术GradeⅡ急性结石性胆囊炎患者(对照组)及52例行PTGD后择期腹腔镜胆囊切除术GradeⅡ急性结石性胆囊炎患者(观察组),比较两组手术时间、术中出血量、术后肛门排气时间、术后引流时间、术后住院时间、炎症指标[白细胞(WBC)、中性粒细胞、C反应蛋白(CRP)]、血清淀粉酶(AMY)、肝功能指标[丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、直接胆红素(DBIL)、间接胆红素(IBIL)]、中转开腹及安全性。结果观察组手术时间、术中出血量、术后引流时间、术后住院时间短于对照组(P<0.05);观察组术后72 h WBC、中性粒细胞、CRP低于对照组(P<0.05);观察组术后72 h AMY低于对照组(P<0.05);观察组无中转开腹发生,中转开腹率低于对照组(P<0.05);观察组并发症发生率为3.85%(2/52),低于对照组的15.38%(8/52)(P<0.05)。结论PTGD后择期腹腔镜胆囊切除术治疗GradeⅡ急性结石性胆囊炎,能减少术中出血量,缩短术后引流时间和住院时间,降低中转开腹率,并能促进胆囊炎症消退,安全性高。展开更多
Objective:To investigate the vasculogenic mimicry formation induced by hypoxia in Ⅱ-Ⅲ human glioma cell and the effect of alphastatin peptide suppressing the hypoxia-induced vasculogenic mimicry formation and the me...Objective:To investigate the vasculogenic mimicry formation induced by hypoxia in Ⅱ-Ⅲ human glioma cell and the effect of alphastatin peptide suppressing the hypoxia-induced vasculogenic mimicry formation and the mechanism.Methods:MTT,Transwell and three-dimentional culture were used to detect the proliferation,migration and tubule formation of SHG44.The expression of vascular endothelial growth factor-α(VEGF-α),erythropoietin-producing hepatocellular carcinoma-A2 (EphA2) and matrix metalloproteinases 2 (MMP2) was detected by RT-PCR and Western blotting analysis.Results:The OD 490 in hypoxia group was 0.60±0.06 and in control group was 0.46±0.05.The number of cell migration was 178.71±18.81 in hypoxia group and 85.86±17.92 in control group.The tubule formation was 56.80±12.21 in hypoxia group and 4.20±2.62 in control group.The proliferation,migration and tubule formation in hypoxia group were significantly higher than that in control group.The expression of VEGF-α,EphA2 and MMP2 was upregulated in hypoxia.When various concentrations of alphastatin (100,1 000,10 000 nmol/L) were added to hypoxia group,the numbers of cell migration were 142.57±12.12,92.71±17.68,30.00±7.72 and the tubule formation were 47.71±10.58,18.86±8.40,8.43±5.62.The cell migration and tubule formation were significantly suppressed by alphastatin in a dose-dependent manner.In alphastatin group,the phosphorylation of EphA2 protein (P=0.037,F=4.629) and activation of MMP2 protein (P=0.005,F=9.331) were significantly suppressed but there was no change in VEGF-α protein.Conclusion:Ⅱ-Ⅲ human glioma cell is able to form vasculogenic mimicry induced by hypoxia and alphastatin peptide can suppress the hypoxia-induced vasculogenic mimicry.VEGF-α induced EphA2 phospharilation and MMP2 activation maybe the key pathway to form vasculogenic mimicry.展开更多
文摘目的分析PTGD后择期LC治疗对GradeⅡ急性胆囊炎ACTH、MPO及Cor水平的影响。方法选取2021年12月至2023年5月安徽中科庚玖医院收治的急性胆囊炎患者121例,根据治疗方案分为三组,即甲组(急诊行LC治疗,未行PTGD)38例、乙组(PTGD引流管拔出后72 h后行LC,早期)43例和丙组(PTGD引流管拔出后14~30 d后行LC,晚期)40例。对比三组手术情况、炎症因子、肝功能、ACTH、MP、Cor水平及并发症发生率。结果甲组LC手术时长、术后卧床时长及住院天数均长于丙组、乙组,失血量、中转开腹率高于丙组、乙组,差异有统计学意义(P<0.05);丙组LC手术时长、术后卧床时长及住院天数均长于乙组,差异具有统计学意义(P<0.05)。LC术后1 d hs-CRP、PCT、IL-6、ST、ALT、ALP、ACTH、MPO及Cor:甲组>丙组>乙组,差异有统计学意义(P<0.05)。并发症发生率:甲组>丙组>乙组,差异有统计学意义(P<0.05)。结论PTGD后择期LC治疗对GradeⅡ急性胆囊炎ACTH、MPO及Cor水平影响小,且并发症低;而PTGD后早期行LC能有效改善肝功能、炎症因子,且术后应激反应更低,值得临床推广。
文摘目的探讨经皮胆囊穿刺引流(PTGD)后择期腹腔镜胆囊切除术治疗GradeⅡ急性结石性胆囊炎患者的效果。方法回顾性选取2017年8月至2020年6月尉氏县中心医院收治的52例行急诊腹腔镜胆囊切除术GradeⅡ急性结石性胆囊炎患者(对照组)及52例行PTGD后择期腹腔镜胆囊切除术GradeⅡ急性结石性胆囊炎患者(观察组),比较两组手术时间、术中出血量、术后肛门排气时间、术后引流时间、术后住院时间、炎症指标[白细胞(WBC)、中性粒细胞、C反应蛋白(CRP)]、血清淀粉酶(AMY)、肝功能指标[丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、直接胆红素(DBIL)、间接胆红素(IBIL)]、中转开腹及安全性。结果观察组手术时间、术中出血量、术后引流时间、术后住院时间短于对照组(P<0.05);观察组术后72 h WBC、中性粒细胞、CRP低于对照组(P<0.05);观察组术后72 h AMY低于对照组(P<0.05);观察组无中转开腹发生,中转开腹率低于对照组(P<0.05);观察组并发症发生率为3.85%(2/52),低于对照组的15.38%(8/52)(P<0.05)。结论PTGD后择期腹腔镜胆囊切除术治疗GradeⅡ急性结石性胆囊炎,能减少术中出血量,缩短术后引流时间和住院时间,降低中转开腹率,并能促进胆囊炎症消退,安全性高。
基金Supported in Part by a Grant from the National Nature Science Foundation of China(No.30672126)
文摘Objective:To investigate the vasculogenic mimicry formation induced by hypoxia in Ⅱ-Ⅲ human glioma cell and the effect of alphastatin peptide suppressing the hypoxia-induced vasculogenic mimicry formation and the mechanism.Methods:MTT,Transwell and three-dimentional culture were used to detect the proliferation,migration and tubule formation of SHG44.The expression of vascular endothelial growth factor-α(VEGF-α),erythropoietin-producing hepatocellular carcinoma-A2 (EphA2) and matrix metalloproteinases 2 (MMP2) was detected by RT-PCR and Western blotting analysis.Results:The OD 490 in hypoxia group was 0.60±0.06 and in control group was 0.46±0.05.The number of cell migration was 178.71±18.81 in hypoxia group and 85.86±17.92 in control group.The tubule formation was 56.80±12.21 in hypoxia group and 4.20±2.62 in control group.The proliferation,migration and tubule formation in hypoxia group were significantly higher than that in control group.The expression of VEGF-α,EphA2 and MMP2 was upregulated in hypoxia.When various concentrations of alphastatin (100,1 000,10 000 nmol/L) were added to hypoxia group,the numbers of cell migration were 142.57±12.12,92.71±17.68,30.00±7.72 and the tubule formation were 47.71±10.58,18.86±8.40,8.43±5.62.The cell migration and tubule formation were significantly suppressed by alphastatin in a dose-dependent manner.In alphastatin group,the phosphorylation of EphA2 protein (P=0.037,F=4.629) and activation of MMP2 protein (P=0.005,F=9.331) were significantly suppressed but there was no change in VEGF-α protein.Conclusion:Ⅱ-Ⅲ human glioma cell is able to form vasculogenic mimicry induced by hypoxia and alphastatin peptide can suppress the hypoxia-induced vasculogenic mimicry.VEGF-α induced EphA2 phospharilation and MMP2 activation maybe the key pathway to form vasculogenic mimicry.
