Background Non-small cell lung cancer （NSCLC） is the leading cause of cancer mortality worldwide. Platelet activation may play an important role in pathologic progress in lung cancer. In this study, we aimed to clar...Background Non-small cell lung cancer （NSCLC） is the leading cause of cancer mortality worldwide. Platelet activation may play an important role in pathologic progress in lung cancer. In this study, we aimed to clarify the influence of activated platelets on lung cancer generation and growth, and the relationship among these functional and ultrastructural chanqes of platelets and the severity of pathoeenetic condition in these Datients with NSCLC.Methods One hundred and thirty-six cases of patients with pathologically confirmed NSCLC were included in this study. Fifty-four healthy people were enrolled as controls. The change of ultra microstructure and activity of blood platelets were observed under the transmission and scanning electron microscope. Simultaneous determination of plasma granule membrane protein 140 （GMP-140） was made.Results Transmission electron microscopy showed remarkable changes of ultra microstructure of platelets in patients with NSCLC, including swelling, increase of α-granules, vesicles, and glycogenosome. Scanning electron microscopy showed many more surface processes and wrinkles on platelets in patients with NSCLC. The reference plasma levels of GMP-140 of healthy controls were （18.2±2.7） μg/L. The plasma levels of GMP-140 in patients with NSCLC were （47.8±12.3） μg/L, which were much higher than those of the controls. There was a medium positive correlation between plasma levels of GMP-140 and amount of α-granules （r=0.514, P 〈0.01） and a high positive correlation between plasma levels of GMP-140 and area of platelet （r=0.84, P 〈0.01） in patients with NSCLC. The Kaplan-Meier survival curve analysis showed significant shift to the left in patients with NSCLC whose a-granules per platelet were 19 or more compared to those 18 or less （Log rank statistic, X^2= 17.38, P〈0.01）.Conclusions There are significant activated changes of ultra microstructure and increased activity of blood platelets in patients with NSCLC. These activated platelets may play an important role in the generation and growth of lung cancer. These changes can be used as a diagnostic index of severity, progression, and prognosis of NSCLC.展开更多
Objective To investigate the dynamic changes and the clinic significance of coagulation and fibrinolysis markers in patients with severe craniocerebral trauma.Methods Thirty three consecutive patients [Glasgow coma sc...Objective To investigate the dynamic changes and the clinic significance of coagulation and fibrinolysis markers in patients with severe craniocerebral trauma.Methods Thirty three consecutive patients [Glasgow coma scale (GCS) ≤8 and APACHE Ⅱ≥10] including 10 women and 23 men with age of (46.3±16.3) years old and with severe craniocerebral trauma were enrolled in this study.Endothelin(ET),alpha-granular membrane protein-140(GMP-140) and D-dimer(DD) were measured at 1st day,3rd day and 7th day of admission,head computerized tomography(CT) was performed on each patient.Twenty healthy people with similar age were as control.ET was determined by the reagent kit which from Shanghai Shenjia company and GC-1200 γ radiatory counter.GMP-140 was determined by the reagent kit which from Suzhou university and PEKINEINMER WIZARD-1470 γ radiatory counter.DD was determined by the reagent kit Shanghai sung biological product company limited and measured by latex agglutination.Results ET,GMP-140 and DD levels were significantly increased at the time of admission[(75.24±26.44)pg/ml,(26.43±13.94)ng/ml,(3.20±0.97)μg/ml,respectively],then gradually decreased.ET and DD levels were significantly higher than control group even after 7 days[(44.66±15.25)pg/ml,(1.35±0.55) μg/ml,respectively],but not for GMP-140.All of the coagulation and fibrinolysis abnormalities markers were negatively correlated with GCS,P<0.05.There were 18 survivors including 8 vegetables and 15 dead including 1 case suffered from disseminated intravascular coagulation(DIC) in this investigation.Conclusions Coagulation and fibrinolysis abnormalities markers were occurred at the very early stage in the patients with severe craniocerebral trauma.The levels of ET,GMP-140 and DD levels were negatively correlated with GCS.展开更多
文摘Background Non-small cell lung cancer （NSCLC） is the leading cause of cancer mortality worldwide. Platelet activation may play an important role in pathologic progress in lung cancer. In this study, we aimed to clarify the influence of activated platelets on lung cancer generation and growth, and the relationship among these functional and ultrastructural chanqes of platelets and the severity of pathoeenetic condition in these Datients with NSCLC.Methods One hundred and thirty-six cases of patients with pathologically confirmed NSCLC were included in this study. Fifty-four healthy people were enrolled as controls. The change of ultra microstructure and activity of blood platelets were observed under the transmission and scanning electron microscope. Simultaneous determination of plasma granule membrane protein 140 （GMP-140） was made.Results Transmission electron microscopy showed remarkable changes of ultra microstructure of platelets in patients with NSCLC, including swelling, increase of α-granules, vesicles, and glycogenosome. Scanning electron microscopy showed many more surface processes and wrinkles on platelets in patients with NSCLC. The reference plasma levels of GMP-140 of healthy controls were （18.2±2.7） μg/L. The plasma levels of GMP-140 in patients with NSCLC were （47.8±12.3） μg/L, which were much higher than those of the controls. There was a medium positive correlation between plasma levels of GMP-140 and amount of α-granules （r=0.514, P 〈0.01） and a high positive correlation between plasma levels of GMP-140 and area of platelet （r=0.84, P 〈0.01） in patients with NSCLC. The Kaplan-Meier survival curve analysis showed significant shift to the left in patients with NSCLC whose a-granules per platelet were 19 or more compared to those 18 or less （Log rank statistic, X^2= 17.38, P〈0.01）.Conclusions There are significant activated changes of ultra microstructure and increased activity of blood platelets in patients with NSCLC. These activated platelets may play an important role in the generation and growth of lung cancer. These changes can be used as a diagnostic index of severity, progression, and prognosis of NSCLC.
文摘Objective To investigate the dynamic changes and the clinic significance of coagulation and fibrinolysis markers in patients with severe craniocerebral trauma.Methods Thirty three consecutive patients [Glasgow coma scale (GCS) ≤8 and APACHE Ⅱ≥10] including 10 women and 23 men with age of (46.3±16.3) years old and with severe craniocerebral trauma were enrolled in this study.Endothelin(ET),alpha-granular membrane protein-140(GMP-140) and D-dimer(DD) were measured at 1st day,3rd day and 7th day of admission,head computerized tomography(CT) was performed on each patient.Twenty healthy people with similar age were as control.ET was determined by the reagent kit which from Shanghai Shenjia company and GC-1200 γ radiatory counter.GMP-140 was determined by the reagent kit which from Suzhou university and PEKINEINMER WIZARD-1470 γ radiatory counter.DD was determined by the reagent kit Shanghai sung biological product company limited and measured by latex agglutination.Results ET,GMP-140 and DD levels were significantly increased at the time of admission[(75.24±26.44)pg/ml,(26.43±13.94)ng/ml,(3.20±0.97)μg/ml,respectively],then gradually decreased.ET and DD levels were significantly higher than control group even after 7 days[(44.66±15.25)pg/ml,(1.35±0.55) μg/ml,respectively],but not for GMP-140.All of the coagulation and fibrinolysis abnormalities markers were negatively correlated with GCS,P<0.05.There were 18 survivors including 8 vegetables and 15 dead including 1 case suffered from disseminated intravascular coagulation(DIC) in this investigation.Conclusions Coagulation and fibrinolysis abnormalities markers were occurred at the very early stage in the patients with severe craniocerebral trauma.The levels of ET,GMP-140 and DD levels were negatively correlated with GCS.
