Raw corn starch granules were hydrolysized by glucoamylase in a chemostat. The hydro- lysis of three different-sized granules shows that smaller granules undergo more hydrolyzation than larger ones. After 78 h, 9700 o...Raw corn starch granules were hydrolysized by glucoamylase in a chemostat. The hydro- lysis of three different-sized granules shows that smaller granules undergo more hydrolyzation than larger ones. After 78 h, 9700 of the granules was hydrolysized with diameter between 0.15 mm and 0.3 mm at 50 ℃. When corn starch concentration increased from 100 g/L to 250 g/L, the amount of reducing sugar produced was proportional to the initial substrate concentration and no substrate inhibition phenomenon appeared. In order to study the product inhibition exactly, the product from hydrolysis reaction itself was added into the hydrolysis system at the beginning of starch hydrolysis. Product inhibition with different quantities of product added were studied in the initial several hours, during which period enzyme inactivation could be neglected and product inhibition could be studied separately. The experiments indicate that product inhibition happens when the additional quantity exceeds 9.56 g/L.展开更多
A key to tackling the coronavirus disease 2019(COVID-19)pandemic is to understand how severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)manages to outsmart host antiviral defense mechanisms.Stress granules(S...A key to tackling the coronavirus disease 2019(COVID-19)pandemic is to understand how severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)manages to outsmart host antiviral defense mechanisms.Stress granules(SGs),which are assembled during viral infection and function to sequester host and viral m RNAs and proteins,are part of the antiviral responses.Here,we show that the SARS-Co V-2 nucleocapsid(N)protein,an RNA binding protein essential for viral production,interacted with RasGTPase-activating protein SH3-domain-binding protein(G3 BP)and disrupted SG assembly,both of which require intrinsically disordered region1(IDR1)in N protein.The N protein partitioned into SGs through liquid-liquid phase separation with G3 BP,and blocked the interaction of G3 BP1 with other SG-related proteins.Moreover,the N protein domains important for phase separation with G3 BP and SG disassembly were required for SARS-Co V-2 viral production.We propose that N protein-mediated SG disassembly is crucial for SARS-Co V-2 production.展开更多
This paper deals with a comparative research between two processes of granulation, namely mini-pelletized sintering （MPS） and hybrid pelletized sintering （HPS）, focusing on aspects such as the balling effect, prod...This paper deals with a comparative research between two processes of granulation, namely mini-pelletized sintering （MPS） and hybrid pelletized sintering （HPS）, focusing on aspects such as the balling effect, production, quality, and mineralography and metallurgical performance of sinter. The results indicate that both methods can result in a satisfying capability in balling effect and metallurgical performance of sinter, qualified to meet the plant production requirements, but when the granulation time and granulating moisture are set to around 6 minutes and 7.0% respectively, MPS is better than HPS.展开更多
The photo bioreaction combined with flow and mass transfer is simulated with pore-scale lattice Boltzmann （LB） method, which is the scenario of a bioreactor filled with a porous granule immobilized photosynthetic ba...The photo bioreaction combined with flow and mass transfer is simulated with pore-scale lattice Boltzmann （LB） method, which is the scenario of a bioreactor filled with a porous granule immobilized photosynthetic bacteria cells for hydrogen production. The quartet structure generation set （QSGS） is used to generate porous structure of the immobilized granule. The effects of porosity of the immobilized granule on flow and concentration fields as well as the hydrogen production performance are investi- gated. Higher porosity facilitates the substrate solution smoothly flowing through the porous granule with increasing velocity, and thus results in higher product concentration inside the immobilized gran- ule. Additionally, the substrate consumption efficiency increases, while hydrogen yield slightly decreases with increasing porosity, and they tend to stable for the porosity larger than 0.5. Furthermore, the LB numerical results have a good agreement with the experimental results. It is demonstrated that the pore-scale LB simulation method coupling with QSGS is available to simulate the photo hydrogen produc- tion in the hioreactor with porous immobilized granules.展开更多
文摘Raw corn starch granules were hydrolysized by glucoamylase in a chemostat. The hydro- lysis of three different-sized granules shows that smaller granules undergo more hydrolyzation than larger ones. After 78 h, 9700 of the granules was hydrolysized with diameter between 0.15 mm and 0.3 mm at 50 ℃. When corn starch concentration increased from 100 g/L to 250 g/L, the amount of reducing sugar produced was proportional to the initial substrate concentration and no substrate inhibition phenomenon appeared. In order to study the product inhibition exactly, the product from hydrolysis reaction itself was added into the hydrolysis system at the beginning of starch hydrolysis. Product inhibition with different quantities of product added were studied in the initial several hours, during which period enzyme inactivation could be neglected and product inhibition could be studied separately. The experiments indicate that product inhibition happens when the additional quantity exceeds 9.56 g/L.
基金supported by the National Natural Science Foundation of China(81830004,31970755,and 31970173)the Local Grant(608285568031)。
文摘A key to tackling the coronavirus disease 2019(COVID-19)pandemic is to understand how severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)manages to outsmart host antiviral defense mechanisms.Stress granules(SGs),which are assembled during viral infection and function to sequester host and viral m RNAs and proteins,are part of the antiviral responses.Here,we show that the SARS-Co V-2 nucleocapsid(N)protein,an RNA binding protein essential for viral production,interacted with RasGTPase-activating protein SH3-domain-binding protein(G3 BP)and disrupted SG assembly,both of which require intrinsically disordered region1(IDR1)in N protein.The N protein partitioned into SGs through liquid-liquid phase separation with G3 BP,and blocked the interaction of G3 BP1 with other SG-related proteins.Moreover,the N protein domains important for phase separation with G3 BP and SG disassembly were required for SARS-Co V-2 viral production.We propose that N protein-mediated SG disassembly is crucial for SARS-Co V-2 production.
文摘This paper deals with a comparative research between two processes of granulation, namely mini-pelletized sintering （MPS） and hybrid pelletized sintering （HPS）, focusing on aspects such as the balling effect, production, quality, and mineralography and metallurgical performance of sinter. The results indicate that both methods can result in a satisfying capability in balling effect and metallurgical performance of sinter, qualified to meet the plant production requirements, but when the granulation time and granulating moisture are set to around 6 minutes and 7.0% respectively, MPS is better than HPS.
基金financial support provided by the State Key Program of National Natural Science of China (51136007)National Natural Science Funds for Distinguished Young Scholars (50825602)
文摘The photo bioreaction combined with flow and mass transfer is simulated with pore-scale lattice Boltzmann （LB） method, which is the scenario of a bioreactor filled with a porous granule immobilized photosynthetic bacteria cells for hydrogen production. The quartet structure generation set （QSGS） is used to generate porous structure of the immobilized granule. The effects of porosity of the immobilized granule on flow and concentration fields as well as the hydrogen production performance are investi- gated. Higher porosity facilitates the substrate solution smoothly flowing through the porous granule with increasing velocity, and thus results in higher product concentration inside the immobilized gran- ule. Additionally, the substrate consumption efficiency increases, while hydrogen yield slightly decreases with increasing porosity, and they tend to stable for the porosity larger than 0.5. Furthermore, the LB numerical results have a good agreement with the experimental results. It is demonstrated that the pore-scale LB simulation method coupling with QSGS is available to simulate the photo hydrogen produc- tion in the hioreactor with porous immobilized granules.
