Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of grass-roots agricultural technology extension system in Guiyang City. Then we point out some problems in the building o...Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of grass-roots agricultural technology extension system in Guiyang City. Then we point out some problems in the building of grass-roots agricultural technology extension system in Guiyang City: the function of grass-roots agricultural technology extension center weakens; the content of agricultural technology extension is difficult to adapt to farmers' needs for technology; the extension mode of agricultural technology departments does not adapt to the needs of modern agriculture. In order to perfect the building of grass-roots agricultural technology extension system, the countermeasures and recommendations are put forth as follows: strengthening the input of funds, and ensuring that the basic work of public welfare agricultural technology extension is smoothly carried out; innovating upon the system, and improving the function of grass-roots agricultural technology extension center; implementing management on agricultural technology extension personnel's performance, and promoting the extension efficiency; strengthening the building of extension team in rural areas, and cultivating high-quality agricultural technology extension personnel; exploring the advanced service mode to meet farmers' needs.展开更多
System of villagers autonomy is one of the basic political systems in China and one of those systems ensuring that the people are the masters of the country. Northwest minorities generally live in the remote northwest...System of villagers autonomy is one of the basic political systems in China and one of those systems ensuring that the people are the masters of the country. Northwest minorities generally live in the remote northwestern China where economy and education are relatively underdeveloped compared with those in central and eastern China and civil authority has great influence on rural grass-roots democracy as well as social autonomy there,which causes difficulties to the development of rural democracy and social autonomy. Assisting northwest minority regions in overcoming the difficulties of developing grass-roots democracy and social autonomy,carrying out effective measures to properly guide the development and influence of civil authority and finally achieving the construction of new socialist countryside have great significance in ensuring that people there are the masters of their country.展开更多
Based on theories of resource dependence and government functions,this paper analyzed benefit interaction between grass-roots government and farmers' specialized cooperatives in Hubei Province. It was intended to ...Based on theories of resource dependence and government functions,this paper analyzed benefit interaction between grass-roots government and farmers' specialized cooperatives in Hubei Province. It was intended to make clear the path dependence relationship between grassroots government and farmers' specialized cooperatives and find out problems in between. Besides,from the perspective of grass-roots government and farmers' specialized cooperatives,it came up with corresponding recommendations,to promote constant improvement of farmers' specialized cooperatives,and help them to become key forces of leading farmers to get rich,pull agricultural economic development,and promote new socialist countryside construction.展开更多
At present,it is urgent to solve problems of reforming and improving grass-roots water conservancy management mode in rural areas and improving rural water conservancy public service ability. Through analyzing institu...At present,it is urgent to solve problems of reforming and improving grass-roots water conservancy management mode in rural areas and improving rural water conservancy public service ability. Through analyzing institutional changes of grass-roots water conservancy management in rural areas,this paper discussed current situations and outstanding problems in grass-roots water conservancy services in rural areas of Kunming City. On the basis of current situations and problems,it came up with policy recommendations from reform of management institutions,reform of personnel system,construction of human resources,development of rural water use cooperation organizations,and improvement of fund input mechanism,to build rural grass-roots water conservancy service system.展开更多
Background: To explore the relationship between acute stress, social support and sleep disorder in grass-root military personnel, and construct the relational model through structural equation modeling. Methods: A tot...Background: To explore the relationship between acute stress, social support and sleep disorder in grass-root military personnel, and construct the relational model through structural equation modeling. Methods: A total of 2,411 grass-root military personnel were randomly selected by cluster sampling, and administered the Chinese Military Personnel Sleep Disorder Scale, Military Acute Stress Scale and Social Support Rating Scale.Results: The total score of acute stress scale was positively correlated with the total score and factor scores of sleep disorder scale(r=0.209~0.465, P【0.01); The total score of social support scale was positively correlated with the total score of acute stress scale and the total score and factor scores of sleep disorder scale(r=0.356~0.537, P【0.01). The analysis of mediating effects showed that lack of social support partially mediated between acute stress and the factors of sleep disorder. The analysis of structural equation model showed that acute stress not only had a direct effect on sleep disorder(the path coefficient was 0.29, P=0.000), but also on lack of social support(the path coefficient was 0.39, P=0.000); lack of social support had a direct effect on sleep disorder(the path coefficient was 0.48, P=0.000).Conclusions: Acute stress and lack of social support are two significant factors of sleep disorder in grass-root military personnel. Well-established social support could alleviate sleep disorder induced by acute stress. Lack of social support was a partial mediator between acute stress and sleep disorder.展开更多
The integration of party construction and poverty alleviation and development is an important form of achieving poverty alleviation in China. Based on the actual case of the integration of the party branch constructio...The integration of party construction and poverty alleviation and development is an important form of achieving poverty alleviation in China. Based on the actual case of the integration of the party branch construction of the Institute of Targeted Poverty Alleviation and Development of Yunnan University of Finance and Economics and the construction of a third-party evaluation team for targeted poverty alleviation,this paper elaborated the practical significance and integration path design of the grassroots party construction and targeted poverty alleviation thirdparty evaluation team construction in colleges and universities. Besides,it analyzed the typical case of the party branch in the process of building the party branch of the ' double leader' teachers in the national colleges and universities,and vigorously implementing the development of the party branch construction and the third-party evaluation team for targeted poverty alleviation,and promoting double integration and double promotion. Finally,it came up with recommendations for further strengthening the development of the party branch construction and the thirdparty evaluation team for targeted poverty alleviation( including strengthening the party construction responsibility,promoting the solid foundation,promoting deep integration,strengthening mutual promotion,gathering poverty alleviation talents,and strengthen intellectual support),so as to form a new model of poverty alleviation through party construction and practicing the lofty purpose of serving the poor household wholeheartedly,and provide a useful reference for the grassroots party construction in China to promote poverty alleviation.展开更多
[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistr...[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a "U"-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a "U" shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.展开更多
Estrogen affects the generation and transmission of neuropathic pain,but the specific regulatory mechanism is still unclear.Activation of the N-methyl-D-aspartate acid receptor 1(NMDAR1) plays an important role in t...Estrogen affects the generation and transmission of neuropathic pain,but the specific regulatory mechanism is still unclear.Activation of the N-methyl-D-aspartate acid receptor 1(NMDAR1) plays an important role in the production and maintenance of hyperalgesia and allodynia.The present study was conducted to determine whether a relationship exists between estrogen and NMDAR1 in peripheral nerve pain.A chronic sciatic nerve constriction injury model of chronic neuropathic pain was established in rats.These rats were then subcutaneously injected with 17β-estradiol,the NMDAR1 antagonist D(-)-2-amino-5-phosphonopentanoic acid(AP-5),or both once daily for 15 days.Compared with injured drug na?ve rats,rats with chronic sciatic nerve injury that were administered estradiol showed a lower paw withdrawal mechanical threshold and a shorter paw withdrawal thermal latency,indicating increased sensitivity to mechanical and thermal pain.Estrogen administration was also associated with increased expression of NMDAR1 immunoreactivity(as assessed by immunohistochemistry) and protein(as determined by western blot assay) in spinal dorsal root ganglia.This 17β-estradiol-induced increase in NMDAR1 expression was blocked by co-administration with AP-5,whereas AP-5 alone did not affect NMDAR1 expression.These results suggest that 17β-estradiol administration significantly reduced mechanical and thermal pain thresholds in rats with chronic constriction of the sciatic nerve,and that the mechanism for this increased sensitivity may be related to the upregulation of NMDAR1 expression in dorsal root ganglia.展开更多
[Objective] To investigate the effects of rooting agents on the growth and photosynthetic traits of Sudan grasses. [ Method] The responses of the growth and some photosynthetic traits of Sudan grasses to three NK II r...[Objective] To investigate the effects of rooting agents on the growth and photosynthetic traits of Sudan grasses. [ Method] The responses of the growth and some photosynthetic traits of Sudan grasses to three NK II rooting agents were determined according to the randomized complete block design. [ Result] Compared to control treatment, the Sudan grass treated by NK 11-298 showed higher chlorophyll a and carotenoid contents, higher chlorophyll fluorescence parameter values ( PSII quantum efficiency: ФPSII; photochemical quenching: qP; and electron transport rate: ETR), higher maximum photosynthetic rate (Pmax) and respiration rate ( Rd), and the highest biomass accumulation. The chlorophyU fluorescence parameter values of Sudan grasses treated by NK 11-888 and NK 11-16 were higher than those of control treatment ( P 〈 0.05), and other indexes had no significant difference. [ Conclusion] The rooting agents should be selected carefully according to their practice aims because of sensibility of Sudan grass to rooting agents.展开更多
In order to provide theoretical basis and technical support for the afforestation and artificial water supply of P.szechuanica in arid areas,the characteristics of water consumption of P.szechuanica were explored,and ...In order to provide theoretical basis and technical support for the afforestation and artificial water supply of P.szechuanica in arid areas,the characteristics of water consumption of P.szechuanica were explored,and the law of water demand of P.szechuanica was grasped.In this paper,potted seedlings of 1-0 rooted cuttings of P.szechuanica were taken as research objects,and change situation of water consumption under different water control gradients was measured regularly by using weighing method,further analyzing dynamic change of water consumption of P.szechuanica and revealing water demand law of 1-0 rooted cuttings of P.szechuanica.The results showed that total change of water consumption of 1-0 rooted cuttings of P.szechuanica had"slow-fast-slow-fast"double-peak trend in the growth period of the current year,and corresponded with univariate linear relation(R^(2)=0.7137),with significant difference.In whole growth period,water consumption in August was the highest,which was 2.7 times of that in June and July and 1.5 times of that after September.In different water control treatments,the dynamic changes of daily and monthly water consumption were significantly different.In seven water control treatments,monthly water consumption was between(6315.95±1690.70)and(10105.28±3065.30)g/month,and mean was(8211.07±2308.23)g/month.With intensification of water control treatment,water consumption increased,but there was no seedling death due to water shortage.P.szechuanica has great plasticity in water demand,and can survive in both arid and humid environments.Meanwhile,it is revealed that P.szechuanica is the most widely distributed tree species in the region.展开更多
Panax ginseng C.A.Mey.is an important plant species used in traditional Chinese medicine,whose primary active ingredient is a ginsenoside.Ginsenoside biosynthesis is not only regulated by transcription factors but als...Panax ginseng C.A.Mey.is an important plant species used in traditional Chinese medicine,whose primary active ingredient is a ginsenoside.Ginsenoside biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural genes.Nonetheless,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary metabolites.Squalene epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as terpenoid.Using ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in ginseng.We first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside biosynthesis.Next,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root clones.Thereafter,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,respectively.Then,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside biosynthesis.Thus,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.展开更多
文摘Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of grass-roots agricultural technology extension system in Guiyang City. Then we point out some problems in the building of grass-roots agricultural technology extension system in Guiyang City: the function of grass-roots agricultural technology extension center weakens; the content of agricultural technology extension is difficult to adapt to farmers' needs for technology; the extension mode of agricultural technology departments does not adapt to the needs of modern agriculture. In order to perfect the building of grass-roots agricultural technology extension system, the countermeasures and recommendations are put forth as follows: strengthening the input of funds, and ensuring that the basic work of public welfare agricultural technology extension is smoothly carried out; innovating upon the system, and improving the function of grass-roots agricultural technology extension center; implementing management on agricultural technology extension personnel's performance, and promoting the extension efficiency; strengthening the building of extension team in rural areas, and cultivating high-quality agricultural technology extension personnel; exploring the advanced service mode to meet farmers' needs.
基金Supported by the Social Sciences Planning Project Launched by Ministry of Education of China in 2011-Study on the Informal Institutional Implementation and the Coordinated Development between Economy and Society in the Northwest Minority Areas(11YJA710005)China Scholarship Council([2012]5030)+1 种基金Professor Andrew J.Nathan,Weatherhead East Asian Insti-tute of Columbia UniversityJames D.Seymour,Adjunct Associate Professor at Chinese University of Hong Kong
文摘System of villagers autonomy is one of the basic political systems in China and one of those systems ensuring that the people are the masters of the country. Northwest minorities generally live in the remote northwestern China where economy and education are relatively underdeveloped compared with those in central and eastern China and civil authority has great influence on rural grass-roots democracy as well as social autonomy there,which causes difficulties to the development of rural democracy and social autonomy. Assisting northwest minority regions in overcoming the difficulties of developing grass-roots democracy and social autonomy,carrying out effective measures to properly guide the development and influence of civil authority and finally achieving the construction of new socialist countryside have great significance in ensuring that people there are the masters of their country.
基金Supported by Fundamental Research Funds for the Central Universities(2012RW009)Study on Development Models and Performance of Farmers' specialized cooperatives in Hubei Province
文摘Based on theories of resource dependence and government functions,this paper analyzed benefit interaction between grass-roots government and farmers' specialized cooperatives in Hubei Province. It was intended to make clear the path dependence relationship between grassroots government and farmers' specialized cooperatives and find out problems in between. Besides,from the perspective of grass-roots government and farmers' specialized cooperatives,it came up with corresponding recommendations,to promote constant improvement of farmers' specialized cooperatives,and help them to become key forces of leading farmers to get rich,pull agricultural economic development,and promote new socialist countryside construction.
基金Supported by Philosophy and Social Science Planning Project of Yunnan Province(JD2015YB30)
文摘At present,it is urgent to solve problems of reforming and improving grass-roots water conservancy management mode in rural areas and improving rural water conservancy public service ability. Through analyzing institutional changes of grass-roots water conservancy management in rural areas,this paper discussed current situations and outstanding problems in grass-roots water conservancy services in rural areas of Kunming City. On the basis of current situations and problems,it came up with policy recommendations from reform of management institutions,reform of personnel system,construction of human resources,development of rural water use cooperation organizations,and improvement of fund input mechanism,to build rural grass-roots water conservancy service system.
基金supported by the special fund of applied military mental health project of the China, the Prevention and Treatment Centre for Psychological Diseases of PLA in the PLA 102nd Hospital, Public Health Division of Joint Logistics Department of Nanjing Military Command
文摘Background: To explore the relationship between acute stress, social support and sleep disorder in grass-root military personnel, and construct the relational model through structural equation modeling. Methods: A total of 2,411 grass-root military personnel were randomly selected by cluster sampling, and administered the Chinese Military Personnel Sleep Disorder Scale, Military Acute Stress Scale and Social Support Rating Scale.Results: The total score of acute stress scale was positively correlated with the total score and factor scores of sleep disorder scale(r=0.209~0.465, P【0.01); The total score of social support scale was positively correlated with the total score of acute stress scale and the total score and factor scores of sleep disorder scale(r=0.356~0.537, P【0.01). The analysis of mediating effects showed that lack of social support partially mediated between acute stress and the factors of sleep disorder. The analysis of structural equation model showed that acute stress not only had a direct effect on sleep disorder(the path coefficient was 0.29, P=0.000), but also on lack of social support(the path coefficient was 0.39, P=0.000); lack of social support had a direct effect on sleep disorder(the path coefficient was 0.48, P=0.000).Conclusions: Acute stress and lack of social support are two significant factors of sleep disorder in grass-root military personnel. Well-established social support could alleviate sleep disorder induced by acute stress. Lack of social support was a partial mediator between acute stress and sleep disorder.
基金Supported by the Construction Project of Party Branch Secretary’s Studio of "Double Leader" Teachers in Colleges and Universities of the Ministry of Education of Chinathe Project of Party Construction Research of Yunnan University of Finance and Economics
文摘The integration of party construction and poverty alleviation and development is an important form of achieving poverty alleviation in China. Based on the actual case of the integration of the party branch construction of the Institute of Targeted Poverty Alleviation and Development of Yunnan University of Finance and Economics and the construction of a third-party evaluation team for targeted poverty alleviation,this paper elaborated the practical significance and integration path design of the grassroots party construction and targeted poverty alleviation thirdparty evaluation team construction in colleges and universities. Besides,it analyzed the typical case of the party branch in the process of building the party branch of the ' double leader' teachers in the national colleges and universities,and vigorously implementing the development of the party branch construction and the third-party evaluation team for targeted poverty alleviation,and promoting double integration and double promotion. Finally,it came up with recommendations for further strengthening the development of the party branch construction and the thirdparty evaluation team for targeted poverty alleviation( including strengthening the party construction responsibility,promoting the solid foundation,promoting deep integration,strengthening mutual promotion,gathering poverty alleviation talents,and strengthen intellectual support),so as to form a new model of poverty alleviation through party construction and practicing the lofty purpose of serving the poor household wholeheartedly,and provide a useful reference for the grassroots party construction in China to promote poverty alleviation.
基金Supported by Natural Science Foundation of Ningxia Hui Autonomous Region(NZ0932)National Key Technology R & D Program of China(20068AI06A15-11)~~
文摘[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a "U"-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a "U" shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.
基金supported by the Youth Shihezi University Applied Basic Research Project of China,No.2015ZRKYQ-LH19
文摘Estrogen affects the generation and transmission of neuropathic pain,but the specific regulatory mechanism is still unclear.Activation of the N-methyl-D-aspartate acid receptor 1(NMDAR1) plays an important role in the production and maintenance of hyperalgesia and allodynia.The present study was conducted to determine whether a relationship exists between estrogen and NMDAR1 in peripheral nerve pain.A chronic sciatic nerve constriction injury model of chronic neuropathic pain was established in rats.These rats were then subcutaneously injected with 17β-estradiol,the NMDAR1 antagonist D(-)-2-amino-5-phosphonopentanoic acid(AP-5),or both once daily for 15 days.Compared with injured drug na?ve rats,rats with chronic sciatic nerve injury that were administered estradiol showed a lower paw withdrawal mechanical threshold and a shorter paw withdrawal thermal latency,indicating increased sensitivity to mechanical and thermal pain.Estrogen administration was also associated with increased expression of NMDAR1 immunoreactivity(as assessed by immunohistochemistry) and protein(as determined by western blot assay) in spinal dorsal root ganglia.This 17β-estradiol-induced increase in NMDAR1 expression was blocked by co-administration with AP-5,whereas AP-5 alone did not affect NMDAR1 expression.These results suggest that 17β-estradiol administration significantly reduced mechanical and thermal pain thresholds in rats with chronic constriction of the sciatic nerve,and that the mechanism for this increased sensitivity may be related to the upregulation of NMDAR1 expression in dorsal root ganglia.
基金supported by the Science Research Development Fund of Tianjin Agricultural University(2008D004)the National Natural Science Foundation(30900191)
文摘[Objective] To investigate the effects of rooting agents on the growth and photosynthetic traits of Sudan grasses. [ Method] The responses of the growth and some photosynthetic traits of Sudan grasses to three NK II rooting agents were determined according to the randomized complete block design. [ Result] Compared to control treatment, the Sudan grass treated by NK 11-298 showed higher chlorophyll a and carotenoid contents, higher chlorophyll fluorescence parameter values ( PSII quantum efficiency: ФPSII; photochemical quenching: qP; and electron transport rate: ETR), higher maximum photosynthetic rate (Pmax) and respiration rate ( Rd), and the highest biomass accumulation. The chlorophyU fluorescence parameter values of Sudan grasses treated by NK 11-888 and NK 11-16 were higher than those of control treatment ( P 〈 0.05), and other indexes had no significant difference. [ Conclusion] The rooting agents should be selected carefully according to their practice aims because of sensibility of Sudan grass to rooting agents.
基金Supported by Natural Science Foundation of Tibet Autonomous Region(XZ2019ZRG-58)Forestry Discipline Innovation Team Construction Project of Tibet Agriculture&Animal Husbandry University(Tibet Financial Forecast:2020-001).
文摘In order to provide theoretical basis and technical support for the afforestation and artificial water supply of P.szechuanica in arid areas,the characteristics of water consumption of P.szechuanica were explored,and the law of water demand of P.szechuanica was grasped.In this paper,potted seedlings of 1-0 rooted cuttings of P.szechuanica were taken as research objects,and change situation of water consumption under different water control gradients was measured regularly by using weighing method,further analyzing dynamic change of water consumption of P.szechuanica and revealing water demand law of 1-0 rooted cuttings of P.szechuanica.The results showed that total change of water consumption of 1-0 rooted cuttings of P.szechuanica had"slow-fast-slow-fast"double-peak trend in the growth period of the current year,and corresponded with univariate linear relation(R^(2)=0.7137),with significant difference.In whole growth period,water consumption in August was the highest,which was 2.7 times of that in June and July and 1.5 times of that after September.In different water control treatments,the dynamic changes of daily and monthly water consumption were significantly different.In seven water control treatments,monthly water consumption was between(6315.95±1690.70)and(10105.28±3065.30)g/month,and mean was(8211.07±2308.23)g/month.With intensification of water control treatment,water consumption increased,but there was no seedling death due to water shortage.P.szechuanica has great plasticity in water demand,and can survive in both arid and humid environments.Meanwhile,it is revealed that P.szechuanica is the most widely distributed tree species in the region.
基金This work was supported by an award from the Department of Science and Technology of Jilin Province(20210402043GH and 20210204063YY).
文摘Panax ginseng C.A.Mey.is an important plant species used in traditional Chinese medicine,whose primary active ingredient is a ginsenoside.Ginsenoside biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural genes.Nonetheless,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary metabolites.Squalene epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as terpenoid.Using ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in ginseng.We first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside biosynthesis.Next,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root clones.Thereafter,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,respectively.Then,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside biosynthesis.Thus,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.