Breast cancer (BC) is the most common malignancy in women and is second to lung cancer in terms of cancer mortality. Treatment of BC remains a challenge as current therapies are limited by toxicity and drug resistance...Breast cancer (BC) is the most common malignancy in women and is second to lung cancer in terms of cancer mortality. Treatment of BC remains a challenge as current therapies are limited by toxicity and drug resistance. Graviola (Annona muricata) is a tree that grows in the tropics of North and South America. Traditionally, the leaves and stems from the graviola tree have been used for a wide range of human diseases including cancer.?In vitro?and?in vivo?studies demonstrate anticancer activity in BC however clinical studies are lacking. We present the first case demonstrating clinical benefit without side effects using graviola in a patient with BC whose disease was refractory to multiple lines of chemotherapy including anthracyclines and taxanes.展开更多
Objective: To identify the phytochemical compounds from Annona muricata(A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata...Objective: To identify the phytochemical compounds from Annona muricata(A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata leaves were successively extracted by soxhlet method using n-hexane, ethyl acetate and methanol, and decocted with water. Each extract was analysed by gas chromatography mass spectrometry(GCMS) and characterized with Wiley and NIST library searches. Anti-proliferative activity of each extract was evaluated on MCF7 and MDA-MB-231 breast cancer cells using MTT assay. Results: The GCMS analysis of different solvent extracts of A. muricata leaves showed presence of different chemical groups of compounds such as steroids, terpenoids, phenolic compounds, sugars, sugars alcohol and others including vitamin E. Ethyl acetate leaves extract exhibited the lowest IC_(50) value on the MDA-MB-231 breast cancer cell and n-hexane leaves extract showed the the lowest IC_(50) value on the MCF-7 breast cancer cell. Conclusion: Steroids and phenolic compounds were the main phytocompound groups identified from all A. muricata leaves extracts. The antiproliferative activity of n-hexane and ethyl acetate extract towards breast cancer MCF7 and MDA-MB-231 respectively might be due to the presence of biologically active compounds in the extracts, hence, providing some scientific evidences of the effectiveness of its traditional usages.展开更多
文摘Breast cancer (BC) is the most common malignancy in women and is second to lung cancer in terms of cancer mortality. Treatment of BC remains a challenge as current therapies are limited by toxicity and drug resistance. Graviola (Annona muricata) is a tree that grows in the tropics of North and South America. Traditionally, the leaves and stems from the graviola tree have been used for a wide range of human diseases including cancer.?In vitro?and?in vivo?studies demonstrate anticancer activity in BC however clinical studies are lacking. We present the first case demonstrating clinical benefit without side effects using graviola in a patient with BC whose disease was refractory to multiple lines of chemotherapy including anthracyclines and taxanes.
基金funded by the Universiti Sains Malaysia Short Term Grant(304/PPSP/6313322)
文摘Objective: To identify the phytochemical compounds from Annona muricata(A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata leaves were successively extracted by soxhlet method using n-hexane, ethyl acetate and methanol, and decocted with water. Each extract was analysed by gas chromatography mass spectrometry(GCMS) and characterized with Wiley and NIST library searches. Anti-proliferative activity of each extract was evaluated on MCF7 and MDA-MB-231 breast cancer cells using MTT assay. Results: The GCMS analysis of different solvent extracts of A. muricata leaves showed presence of different chemical groups of compounds such as steroids, terpenoids, phenolic compounds, sugars, sugars alcohol and others including vitamin E. Ethyl acetate leaves extract exhibited the lowest IC_(50) value on the MDA-MB-231 breast cancer cell and n-hexane leaves extract showed the the lowest IC_(50) value on the MCF-7 breast cancer cell. Conclusion: Steroids and phenolic compounds were the main phytocompound groups identified from all A. muricata leaves extracts. The antiproliferative activity of n-hexane and ethyl acetate extract towards breast cancer MCF7 and MDA-MB-231 respectively might be due to the presence of biologically active compounds in the extracts, hence, providing some scientific evidences of the effectiveness of its traditional usages.
