Comparative QTL Mapping of Resistance to Gray Leaf Spot in Maize Based on Bioinformatics

The integration QTL map for gray leaf spot resistance in maize was constructed by compiling a total of 57 QTLs available with genetic map IBM2 2005 neighbors as reference. Twenty-six ＂real QTLs＂ and seven consensus QTLs were identified by refining these 57 QTLs using overview and meta-analysis approaches. Seven consensus QTLs were found on chromosomes 1.06, 2.06, 3.04, 4.06, 4.08, 5.03, and 8.06, and the map coordinates were 552.53,425.72, 279.20, 368.97, 583.21, 308.68 and 446.14 cM, respectively. Using a synteny conservation approach based on comparative mapping between the maize genetic map and rice physical map, a total of 69 rice and maize resistance genes collected from websites Gramene and MaizeGDB were projected onto the maize genetic map IBM2 2005 neighbors, and 2 （Rgene32, htl）, 4 （RgeneS, rp3, scmv2, wsm2）, and 4 （ht2, Rgene6, Rgene8 and Rgene7） positional candidate genes were found in three consensus QTLs on chromosomes 2.06, 3.04, and 8.06, respectively. The results suggested that the combination of meta-analysis of gray leaf spot in maize and sequence homologous comparison between maize and rice could be an efficient strategy for identifying major QTLs and corresponding candidate genes for the gray leaf spot.

Dispersal routes of Cercospora zeina causing maize gray leaf spot in China

The gray leaf spot caused by Cercospora zeina has become a serious disease in maize in China.The isolates of C.zeina from Yunnan,Sichuan,Guizhou,Hubei,Chongqing,Gansu,and Shaanxi were collected.From those,127 samples were used for genetic diversity analysis based on inter-simple sequence repeat(ISSR)and 108 samples were used for multi-gene sequence analysis based on five gene fragments.The results indicated that populations of C.zeina were differentiated with a relatively high genetic level and were classified into two major groups and seven subgroups.The intra-population genetic differentiation of C.zeina is the leading cause of population variation in China,and interpopulation genetic similarity is closely related to the colonization time and spread direction.The multi-gene sequence analysis of C.zeina isolates demonstrated that there were nine haplotypes.Genetic diversity and multi-gene sequence revealed that Yunnan population of C.zeina,the earliest colonizing in China,had the highest genetic and haplotype diversity and had experienced an expansion event.With the influence of the southwest monsoon in the Indian Ocean,C.zeina from Yunnan gradually moved to Sichuan,Guizhou,Shaanxi,Gansu,and Chongqing.Meanwhile,C.zeina was transferred directly from the Yunnan into the Hubei Province via seed and then came into Shaanxi,Henan,and Chongqing along with the wind from Hubei.

常规西医疗法联合二冬汤加减治疗糖尿病视网膜病变的临床分析

目的 常规西医疗法联合二冬汤加减治疗糖尿病视网膜病变的影响性分析。方法 选取2021年11月-2022年11月聊城市眼科医院收治的符合研究开展标准的90例糖尿病视网膜病变患者为研究对象,根据患者入院就诊时间先后顺序分为对照组(n=45)、观察组(n=45);对照组给予羟苯磺酸钙胶囊治疗,观察组基于对照组另行二冬汤加减治疗;比较两组治疗有效率、治疗后视功能改变情况、治疗前后中医症状积分与生活质量评分。结果 观察组治疗有效率为95.56%,高于对照组的77.78%,差异有统计学意义(P<0.05);治疗后,观察组D-二聚体水平、视野灰度值、出血斑面积、微血管瘤体积数值低于对照组,差异有统计学意义(P<0.05);治疗前,两组患者的中医症状积分与中文版低视力者生活质量量表评分比较,差异无统计学意义(P>0.05),治疗后,两组中医症状积分降低,中文版低视力者生活质量量表评分提升,且观察组优于对照组,差异有统计学意义(P<0.05)。结论 基于西医疗法联合使用二冬汤加减治疗糖尿病视网膜病变对患者视功能有较好的改善作用,有利于提高患者预后的生活质量。

Genome-wide identification and expression analysis of GDSL esterase/lipase genes in tomato

The GDSL esterase/lipase family contains many functional genes that perform important biological functions in growth and development, morphogenesis, seed oil synthesis, and defense responses in plants. The expression of GDSL esterase/lipase genes can respond to biotic and abiotic stresses. Although GDSL esterase/lipase family genes have been identified and studied in other plants, they have not been identified and their functions remain unclear in tomato. This study is the first to identify 80 GDSL esterase/lipase family genes in tomato, which were named SlGELP1–80. These genes were mapped to their positions on the chromosomes and their physical and chemical properties, gene structure, phylogenetic relationships, collinear relationships, and cis-acting elements were analyzed. The spatiotemporal expression characteristics of the Sl GELP genes in tomato were diverse. In addition, RNA-seq analysis indicated that the expression patterns of the SlGELP genes in tomato differed before and after inoculation with Stemphylium lycopersici. qRT-PCR was used to analyze the expression of five Sl GELP genes after treatments with S. lycopersici, salicylic acid and jasmonic acid. Finally, this study was the first to identify and analyze GDSL esterase/lipase family genes in tomato via bioinformatics approaches, and these findings provide new insights for improving the study of plant disease resistance.