Objective:To investigate the antibacterial activity and photochemicals of five green leafy vegetables against a panel of five bacteria strains.Methods:Disc diffusion method was used to determine the antibacterial acti...Objective:To investigate the antibacterial activity and photochemicals of five green leafy vegetables against a panel of five bacteria strains.Methods:Disc diffusion method was used to determine the antibacterial activity,while kanamycin was used as a reference antibiotic.The phytochemical screening of the extracts was performed using standard methods.Results:All methanol extracts were found active against all the test bacterial strains.Overall maximum extracts shows antibacterial activity which range from 6 to 15 mm.Proteins and carbohydrates was found in all the green leaves,whereas alkaloid.steroids.saponins,flavonoids.tannins were found in most of the test samples.Conclusions:The obtain result suggests that green leafy vegetables have moderate antibacterial activity and contain various pharmacologically active compounds and thus provide the scientific basis for the traditional uses of the studied vegetables in the treatment of bacterial infections.展开更多
Recently, a novel green fluorescent protein eYGFPuv has been identified in the marine organism Chiridius poppei which displays high fluorescence intensity and can be visible by eyes in dark. Although strong green fluo...Recently, a novel green fluorescent protein eYGFPuv has been identified in the marine organism Chiridius poppei which displays high fluorescence intensity and can be visible by eyes in dark. Although strong green fluorescence was achieved in transgenic petunia, 3 expression cassettes (about 8 kb) complicate its application. In this study, to confirm whether 1 expression cassette could be used as a transgenic marker in prokaryotes and eukaryotes, eYGFPuv was cloned into prokaryotic expression vector pET28α-eYGFPuv- His and plant binary expression vector 35S::eYGFPuv. Compared to EGFP, eYGFPuv protein exhibited stronger dazzling green fluorescence in E. coli under excited light at 365 nm and maintains steadily over a long period of time without degradation. When transiently expressed in tobacco leaves, eYGFPuv protein displayed strong green fluorescence. Moreover, the fluorescence of eYGFPuv protein also could be directly observed in living plant, and thus can be used easily as a marker to screen transformed lines in transgenic research. Overall, compared to previous studies on eYGFPuv tandem repeats, our data confirmed that single eYGFPuv sequence still possesses high fluorescence intensity and quenching resistance. Furthermore, because of small size of expression cassette,it is suitable for efficient transformation in both prokaryotic and eukaryotic organisms.展开更多
基金Supported by a grant from the Research Center of the Center for Female Scientific and Medical Colleges,Deanship of Scientific Research,King Saud University
文摘Objective:To investigate the antibacterial activity and photochemicals of five green leafy vegetables against a panel of five bacteria strains.Methods:Disc diffusion method was used to determine the antibacterial activity,while kanamycin was used as a reference antibiotic.The phytochemical screening of the extracts was performed using standard methods.Results:All methanol extracts were found active against all the test bacterial strains.Overall maximum extracts shows antibacterial activity which range from 6 to 15 mm.Proteins and carbohydrates was found in all the green leaves,whereas alkaloid.steroids.saponins,flavonoids.tannins were found in most of the test samples.Conclusions:The obtain result suggests that green leafy vegetables have moderate antibacterial activity and contain various pharmacologically active compounds and thus provide the scientific basis for the traditional uses of the studied vegetables in the treatment of bacterial infections.
基金supported by the National Natural Science Foundation of China (31500237)
文摘Recently, a novel green fluorescent protein eYGFPuv has been identified in the marine organism Chiridius poppei which displays high fluorescence intensity and can be visible by eyes in dark. Although strong green fluorescence was achieved in transgenic petunia, 3 expression cassettes (about 8 kb) complicate its application. In this study, to confirm whether 1 expression cassette could be used as a transgenic marker in prokaryotes and eukaryotes, eYGFPuv was cloned into prokaryotic expression vector pET28α-eYGFPuv- His and plant binary expression vector 35S::eYGFPuv. Compared to EGFP, eYGFPuv protein exhibited stronger dazzling green fluorescence in E. coli under excited light at 365 nm and maintains steadily over a long period of time without degradation. When transiently expressed in tobacco leaves, eYGFPuv protein displayed strong green fluorescence. Moreover, the fluorescence of eYGFPuv protein also could be directly observed in living plant, and thus can be used easily as a marker to screen transformed lines in transgenic research. Overall, compared to previous studies on eYGFPuv tandem repeats, our data confirmed that single eYGFPuv sequence still possesses high fluorescence intensity and quenching resistance. Furthermore, because of small size of expression cassette,it is suitable for efficient transformation in both prokaryotic and eukaryotic organisms.
