Warthin’s tumor is the second most frequent neoplasm next to pleomorphic adenoma in the salivary gland. The tumor contains the epithelial oncocyte cells with the presence of rich-mitochondria and their surrounding ab...Warthin’s tumor is the second most frequent neoplasm next to pleomorphic adenoma in the salivary gland. The tumor contains the epithelial oncocyte cells with the presence of rich-mitochondria and their surrounding abundant lymphocytes. A relatively new disease entity of IgG4-related disease frequently occurs in the salivary gland. However, the coexistence of Warthin’s tumor and IgG4-related disease is scarcely observed. We have recently experienced a rare case of Warthin’s tumor with IgG4-related sialadenitis. A 51-year-old man presented to our hospital, complaining of a mass with right submandibular tenderness and spontaneous pain. A computed tomography scan of the cervical region revealed a suspicion of lymph node proliferative disease, including malignant lymphoma. Elevated serum levels of IL-2R: 1843 U/ml (reference value 122 - 496 U/ml), IgG: 3430 mg/dl (reference value 861 - 1747 mg/dl), and IgG4: 3140 mg/dl (reference value 11 - 121 mg/dl) were observed. Other laboratory data showed within normal ranges. The cervical tumor was diagnosed as Warthin’s tumor by the findings of fine-needle aspiration cytology and biopsy examination. Immunohistochemistry revealed numerous IgG4- and IgG-positive cells with fibrosis surrounding the epithelial component of Warthin’s tumor, suggesting IgG4-rerated sialadenitis. Finally, we diagnosed the cervical tumor as Warthin tumor with IgG4-related sialadenitis. This is the second report describing a case of Warthin’s tumor with possible involvement of IgG4-related sialadenitis.展开更多
This paper is concerned with the following fourth-order three-point boundary value problem , where , we discuss the existence of positive solutions to the above problem by applying to the fixed point theory in cones a...This paper is concerned with the following fourth-order three-point boundary value problem , where , we discuss the existence of positive solutions to the above problem by applying to the fixed point theory in cones and iterative technique.展开更多
The possible exotic nuclear properties in the neutron-rich Ca,Ni,Zr,and Sn isotopes are examined with the continuum Skyrme Hartree-Fock-Bogoliubov theory in the framework of the Green’s function method.The pairing co...The possible exotic nuclear properties in the neutron-rich Ca,Ni,Zr,and Sn isotopes are examined with the continuum Skyrme Hartree-Fock-Bogoliubov theory in the framework of the Green’s function method.The pairing correlation,the couplings with the continuum,and the blocking effects for the unpaired nucleon in odd-A nuclei are properly treated.The Skyrme interaction SLy4 is adopted for the ph channel and the density-dependentinteraction is adopted for the pp chan-nel,which well reproduce the experimental two-neutron separation energies S_(2n)and one-neutron separation energies Sn.It is found that the criterion S_(n)>0 predicts a neutron drip line with neutron numbers much smaller than those for S_(2n)>0.Owing to the unpaired odd neutron,the neutron pairing energies−E_(pair)in odd-A nuclei are much lower than those in the neighbor-ing even-even nuclei.By investigating the single-particle structures,the possible halo structures in the neutron-rich Ca,Ni,and Sn isotopes are predicted,where sharp increases in the root-mean-square(rms)radii with significant deviations from the traditional rA^(1∕3)rule and diffuse spatial density distributions are observed.Analyzing the contributions of various partial waves to the total neutron densityρlj(r)∕ρ(r)reveals that the orbitals located around the Fermi surface-particularly those with small angular momenta-significantly affect the extended nuclear density and large rms radii.The number of neutrons Nλ(N_(0))occupying above the Fermi surfacen(continuum threshold)is discussed,whose evolution as a function of the mass number A in each isotope is consistent with that of the pairing energy,supporting the key role of the pairing correlation in halo phenomena.展开更多
The pointwise space-time behaviors of the Green’s function and the global solution to the Vlasov-Poisson-Fokker-Planck(VPFP)system in three dimensional space are studied in this paper.It is shown that the Green’s fu...The pointwise space-time behaviors of the Green’s function and the global solution to the Vlasov-Poisson-Fokker-Planck(VPFP)system in three dimensional space are studied in this paper.It is shown that the Green’s function consists of the diffusion waves decaying exponentially in time but algebraically in space,and the singular kinetic waves which become smooth for all(t,x,v)when t>0.Furthermore,we establish the pointwise space-time behaviors of the global solution to the nonlinear VPFP system when the initial data is not necessarily smooth in terms of the Green’s function.展开更多
In this paper,we obtain Green’s functions of two-dimensional(2D)piezoelectric quasicrystal(PQC)in half-space and bimaterials.Based on the elastic theory of QCs,the Stroh formalism is used to derive the general soluti...In this paper,we obtain Green’s functions of two-dimensional(2D)piezoelectric quasicrystal(PQC)in half-space and bimaterials.Based on the elastic theory of QCs,the Stroh formalism is used to derive the general solutions of displacements and stresses.Then,we obtain the analytical solutions of half-space and bimaterial Green’s functions.Besides,the interfacial Green’s function for bimaterials is also obtained in the analytical form.Before numerical studies,a comparative study is carried out to validate the present solutions.Typical numerical examples are performed to investigate the effects of multi-physics loadings such as the line force,the line dislocation,the line charge,and the phason line force.As a result,the coupling effect among the phonon field,the phason field,and the electric field is prominent,and the butterfly-shaped contours are characteristic in 2D PQCs.In addition,the changes of material parameters cause variations in physical quantities to a certain degree.展开更多
By using integral transform methods, the Green(s functions of horizontal harmonic force applied at the interior of the saturated half-space soil are obtained in the paper. The general solutions of the Biot dynamic equ...By using integral transform methods, the Green(s functions of horizontal harmonic force applied at the interior of the saturated half-space soil are obtained in the paper. The general solutions of the Biot dynamic equations in frequency domain are established through the use of Hankel integral transforms technique. Utilizing the above- mentioned general solutions, and the boundary conditions of the surface of the half-space and the continuous conditions at the plane of the horizontal force, the solutions of the boundary value problem can be determined. By the numerical inverse Hankel transforms method, the Green(s functions of the harmonic horizontal force are obtainable. The degenerate case of the results deduced from this paper agrees well with the known results. Two numerical examples are given in the paper.展开更多
为建立一种猪德尔塔病毒(PDCoV)的快速检测方法,本研究根据NCBI公布的PDCoV序列设计了1对特异性引物,利用RT-PCR方法扩增PDCoV的S基因,将其连接至pMD19-T载体上,以构建正确的重组质粒作为标准品,建立SYBR Green Ⅰ实时荧光定量PCR检测方...为建立一种猪德尔塔病毒(PDCoV)的快速检测方法,本研究根据NCBI公布的PDCoV序列设计了1对特异性引物,利用RT-PCR方法扩增PDCoV的S基因,将其连接至pMD19-T载体上,以构建正确的重组质粒作为标准品,建立SYBR Green Ⅰ实时荧光定量PCR检测方法,并对该方法的敏感性、特异性及重复性进行了验证分析。结果显示:该检测方法在标准品浓度为6.26×10^(1)~6.26×10^(8)copies/μL范围内呈良好线性关系,其相关性为0.999,斜率为-4.312;灵敏度良好,检测下限可达6.26×10^(1)copies/μL;特异性良好,对PEDV和TGEV两种猪常见病原均无特异性扩增;重复性好,组内和组间变异系数均小于2.0%;利用该方法对43份临床样品进行检测,PDCoV阳性率为4.6%。结果表明,本研究成功建立了PDCoV的SYBR GreenⅠ实时荧光定量PCR检测方法,为PDCoV快速灵敏的诊断奠定了坚实基础。展开更多
为了建立禽呼肠孤病毒(ARV)快速、敏感的检测方法。本研究利用RT-PCR技术扩增出ARV S1基因中298bp的保守序列,并克隆到p MD18-T载体中作为标准品制作标准曲线,建立了ARV的SYBR Green Ⅰ荧光定量PCR检测方法。该方法检测灵敏度可达4.8...为了建立禽呼肠孤病毒(ARV)快速、敏感的检测方法。本研究利用RT-PCR技术扩增出ARV S1基因中298bp的保守序列,并克隆到p MD18-T载体中作为标准品制作标准曲线,建立了ARV的SYBR Green Ⅰ荧光定量PCR检测方法。该方法检测灵敏度可达4.8×101拷贝,与NDV、AIV、IBV、IBDV、ALV、REV均不发生交叉反应,具有良好的特异性和重复性。结果表明,建立的实时荧光定量PCR具有特异、敏感、快速、定量、重复性好等优点,可用于临床ARV的检测。展开更多
The singularities, oscillatory performances and the contributing factors to the 3-'D translating-pulsating source Green function of deep-water Havelock form which consists of a local disturbance part and a far-field ...The singularities, oscillatory performances and the contributing factors to the 3-'D translating-pulsating source Green function of deep-water Havelock form which consists of a local disturbance part and a far-field wave-like part, are analyzed systematically. Relative numerical integral methods about the two parts are presented in this paper. An improved method based on LOBATTO rule is used to eliminate singularities caused respectively by infinite discontinuity and jump discontinuous node from the local disturbance part function, which makes the improvement of calculation efficiency and accuracy possible. And variable substitution is applied to remove the singularity existing at the end of the integral interval of the far-field wave-like part function. Two auxiliary techniques such as valid interval calculation and local refinement of integral steps technique in narrow zones near false singularities are applied so as to avoid unnecessary integration of invalid interval and improve integral accordance. Numerical test results have proved the efficiency and accuracy in these integral methods that thus can be applied to calculate hydrodynamic performance of floating structures moving in waves.展开更多
Based on one type of practical Biot's equation and the dynamic-stiffness matrices ofa poroelastic soil layer and half-space, Green's functions were derived for unitformly distributed loads acting on an inclined line...Based on one type of practical Biot's equation and the dynamic-stiffness matrices ofa poroelastic soil layer and half-space, Green's functions were derived for unitformly distributed loads acting on an inclined line in a poroelastie layered site. This analysis overcomes significant problems in wave scattering due to local soil conditions and dynamic soil-structure interaction. The Green's functions can be reduced to the case of an elastic layered site developed by Wolf in 1985. Parametric studies are then carried out through two example problems.展开更多
In this paper, we will introduce how to apply Green's function method to get the pointwise estimates for the solutions of Cauchy problem of nonlinear evolution equations with dissipative structure. First of all, we i...In this paper, we will introduce how to apply Green's function method to get the pointwise estimates for the solutions of Cauchy problem of nonlinear evolution equations with dissipative structure. First of all, we introduce the pointwise estimates of the time-asymptotic shape of the solutions of the isentropic Navier-Stokes equations and show to exhibit the generalized Huygen's principle. Then, for other nonlinear dissipative evolution equations, we will only introduce the result and give some brief explanations. Our approach is based on the detailed analysis of the Green's function of the linearized system and micro-local analysis, such as frequency decomposition and so on.展开更多
This study proposes a Green's function, an essential representation of water-saturated ground under moving excitation, to simulate ground borne vibration from trains. First, general solutions to the governing equatio...This study proposes a Green's function, an essential representation of water-saturated ground under moving excitation, to simulate ground borne vibration from trains. First, general solutions to the governing equations of poroelastic medium are derived by means of integral transform. Secondly, the transmission and reflection matrix approach is used to formulate the relationship between displacement and stress of the stratified ground, which results in the matrix of the Green's function. Then the Green's function is combined into a train-track-ground model, and is verified by typical examples and a field test. Additional simulations show that the computed ground vibration attenuates faster in the immediate vicinity of the track than in the surrounding area. The wavelength of wheel-rail unevenness has a notable effect on computed displacement and pore pressure. The variation of vibration intensity with the depth of ground is significantly influenced by the layering of the strata soil. When the train speed is equal to the velocity of the Rayleigh wave, the Mach cone appears in the simulated wave field. The proposed Green's function is an appropriate representation for a layered ground with shallow ground water table, and will be helpful to understand the dynamic responses of the ground to complicated moving excitation.展开更多
On the basis of a long-term(30 years) field experiment that involved four rotation systems, rice-rice-winter fallow(RRF), rice-rice-ryegrass(RRG), rice-rice-rape(RRP), and rice-rice-milk vetch(RRV), this stu...On the basis of a long-term(30 years) field experiment that involved four rotation systems, rice-rice-winter fallow(RRF), rice-rice-ryegrass(RRG), rice-rice-rape(RRP), and rice-rice-milk vetch(RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16 S r RNA gene. The Chao1 richness and non-parametric Shannon's index increased in all soil samples that received green manure treatments. The communities' structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.展开更多
文摘Warthin’s tumor is the second most frequent neoplasm next to pleomorphic adenoma in the salivary gland. The tumor contains the epithelial oncocyte cells with the presence of rich-mitochondria and their surrounding abundant lymphocytes. A relatively new disease entity of IgG4-related disease frequently occurs in the salivary gland. However, the coexistence of Warthin’s tumor and IgG4-related disease is scarcely observed. We have recently experienced a rare case of Warthin’s tumor with IgG4-related sialadenitis. A 51-year-old man presented to our hospital, complaining of a mass with right submandibular tenderness and spontaneous pain. A computed tomography scan of the cervical region revealed a suspicion of lymph node proliferative disease, including malignant lymphoma. Elevated serum levels of IL-2R: 1843 U/ml (reference value 122 - 496 U/ml), IgG: 3430 mg/dl (reference value 861 - 1747 mg/dl), and IgG4: 3140 mg/dl (reference value 11 - 121 mg/dl) were observed. Other laboratory data showed within normal ranges. The cervical tumor was diagnosed as Warthin’s tumor by the findings of fine-needle aspiration cytology and biopsy examination. Immunohistochemistry revealed numerous IgG4- and IgG-positive cells with fibrosis surrounding the epithelial component of Warthin’s tumor, suggesting IgG4-rerated sialadenitis. Finally, we diagnosed the cervical tumor as Warthin tumor with IgG4-related sialadenitis. This is the second report describing a case of Warthin’s tumor with possible involvement of IgG4-related sialadenitis.
文摘This paper is concerned with the following fourth-order three-point boundary value problem , where , we discuss the existence of positive solutions to the above problem by applying to the fixed point theory in cones and iterative technique.
基金the National Natural Science Foundation of China(No.U2032141)the Open Project of Guangxi Key Laboratory of Nuclear Physics and Nuclear Technology(No.NLK2022-02)+4 种基金the Central Government Guidance Funds for Local Scientific and Technological Development,China(Guike ZY22096024)the Natural Science Foundation of Henan Province(No.202300410479)the Guizhou Provincial Science and Technology Projects(No.ZK[2022]203)the Foundation of Fundamental Research for Young Teachers of Zhengzhou University(No.JC202041041)the Physics Research and Development Program of Zhengzhou University(No.32410217).
文摘The possible exotic nuclear properties in the neutron-rich Ca,Ni,Zr,and Sn isotopes are examined with the continuum Skyrme Hartree-Fock-Bogoliubov theory in the framework of the Green’s function method.The pairing correlation,the couplings with the continuum,and the blocking effects for the unpaired nucleon in odd-A nuclei are properly treated.The Skyrme interaction SLy4 is adopted for the ph channel and the density-dependentinteraction is adopted for the pp chan-nel,which well reproduce the experimental two-neutron separation energies S_(2n)and one-neutron separation energies Sn.It is found that the criterion S_(n)>0 predicts a neutron drip line with neutron numbers much smaller than those for S_(2n)>0.Owing to the unpaired odd neutron,the neutron pairing energies−E_(pair)in odd-A nuclei are much lower than those in the neighbor-ing even-even nuclei.By investigating the single-particle structures,the possible halo structures in the neutron-rich Ca,Ni,and Sn isotopes are predicted,where sharp increases in the root-mean-square(rms)radii with significant deviations from the traditional rA^(1∕3)rule and diffuse spatial density distributions are observed.Analyzing the contributions of various partial waves to the total neutron densityρlj(r)∕ρ(r)reveals that the orbitals located around the Fermi surface-particularly those with small angular momenta-significantly affect the extended nuclear density and large rms radii.The number of neutrons Nλ(N_(0))occupying above the Fermi surfacen(continuum threshold)is discussed,whose evolution as a function of the mass number A in each isotope is consistent with that of the pairing energy,supporting the key role of the pairing correlation in halo phenomena.
基金supported by National Natural Science Foundation of China(11671100 and 12171104)the National Science Fund for Excellent Young Scholars(11922107)Guangxi Natural Science Foundation(2018GXNSFAA138210 and 2019JJG110010)。
文摘The pointwise space-time behaviors of the Green’s function and the global solution to the Vlasov-Poisson-Fokker-Planck(VPFP)system in three dimensional space are studied in this paper.It is shown that the Green’s function consists of the diffusion waves decaying exponentially in time but algebraically in space,and the singular kinetic waves which become smooth for all(t,x,v)when t>0.Furthermore,we establish the pointwise space-time behaviors of the global solution to the nonlinear VPFP system when the initial data is not necessarily smooth in terms of the Green’s function.
基金the National Natural Science Foundation of China(Nos.11972365 and 12102458)。
文摘In this paper,we obtain Green’s functions of two-dimensional(2D)piezoelectric quasicrystal(PQC)in half-space and bimaterials.Based on the elastic theory of QCs,the Stroh formalism is used to derive the general solutions of displacements and stresses.Then,we obtain the analytical solutions of half-space and bimaterial Green’s functions.Besides,the interfacial Green’s function for bimaterials is also obtained in the analytical form.Before numerical studies,a comparative study is carried out to validate the present solutions.Typical numerical examples are performed to investigate the effects of multi-physics loadings such as the line force,the line dislocation,the line charge,and the phason line force.As a result,the coupling effect among the phonon field,the phason field,and the electric field is prominent,and the butterfly-shaped contours are characteristic in 2D PQCs.In addition,the changes of material parameters cause variations in physical quantities to a certain degree.
基金State Natural Science Foundation (59879012) and Doctoral Foundation from State Education Commission (98024832).
文摘By using integral transform methods, the Green(s functions of horizontal harmonic force applied at the interior of the saturated half-space soil are obtained in the paper. The general solutions of the Biot dynamic equations in frequency domain are established through the use of Hankel integral transforms technique. Utilizing the above- mentioned general solutions, and the boundary conditions of the surface of the half-space and the continuous conditions at the plane of the horizontal force, the solutions of the boundary value problem can be determined. By the numerical inverse Hankel transforms method, the Green(s functions of the harmonic horizontal force are obtainable. The degenerate case of the results deduced from this paper agrees well with the known results. Two numerical examples are given in the paper.
文摘为建立一种猪德尔塔病毒(PDCoV)的快速检测方法,本研究根据NCBI公布的PDCoV序列设计了1对特异性引物,利用RT-PCR方法扩增PDCoV的S基因,将其连接至pMD19-T载体上,以构建正确的重组质粒作为标准品,建立SYBR Green Ⅰ实时荧光定量PCR检测方法,并对该方法的敏感性、特异性及重复性进行了验证分析。结果显示:该检测方法在标准品浓度为6.26×10^(1)~6.26×10^(8)copies/μL范围内呈良好线性关系,其相关性为0.999,斜率为-4.312;灵敏度良好,检测下限可达6.26×10^(1)copies/μL;特异性良好,对PEDV和TGEV两种猪常见病原均无特异性扩增;重复性好,组内和组间变异系数均小于2.0%;利用该方法对43份临床样品进行检测,PDCoV阳性率为4.6%。结果表明,本研究成功建立了PDCoV的SYBR GreenⅠ实时荧光定量PCR检测方法,为PDCoV快速灵敏的诊断奠定了坚实基础。
文摘为了建立禽呼肠孤病毒(ARV)快速、敏感的检测方法。本研究利用RT-PCR技术扩增出ARV S1基因中298bp的保守序列,并克隆到p MD18-T载体中作为标准品制作标准曲线,建立了ARV的SYBR Green Ⅰ荧光定量PCR检测方法。该方法检测灵敏度可达4.8×101拷贝,与NDV、AIV、IBV、IBDV、ALV、REV均不发生交叉反应,具有良好的特异性和重复性。结果表明,建立的实时荧光定量PCR具有特异、敏感、快速、定量、重复性好等优点,可用于临床ARV的检测。
基金supported by the National Natural Science Foundation of China (Grant No. 50879090)
文摘The singularities, oscillatory performances and the contributing factors to the 3-'D translating-pulsating source Green function of deep-water Havelock form which consists of a local disturbance part and a far-field wave-like part, are analyzed systematically. Relative numerical integral methods about the two parts are presented in this paper. An improved method based on LOBATTO rule is used to eliminate singularities caused respectively by infinite discontinuity and jump discontinuous node from the local disturbance part function, which makes the improvement of calculation efficiency and accuracy possible. And variable substitution is applied to remove the singularity existing at the end of the integral interval of the far-field wave-like part function. Two auxiliary techniques such as valid interval calculation and local refinement of integral steps technique in narrow zones near false singularities are applied so as to avoid unnecessary integration of invalid interval and improve integral accordance. Numerical test results have proved the efficiency and accuracy in these integral methods that thus can be applied to calculate hydrodynamic performance of floating structures moving in waves.
基金National Natural Science Foundation of China Under Grant No.50378063
文摘Based on one type of practical Biot's equation and the dynamic-stiffness matrices ofa poroelastic soil layer and half-space, Green's functions were derived for unitformly distributed loads acting on an inclined line in a poroelastie layered site. This analysis overcomes significant problems in wave scattering due to local soil conditions and dynamic soil-structure interaction. The Green's functions can be reduced to the case of an elastic layered site developed by Wolf in 1985. Parametric studies are then carried out through two example problems.
基金supported by National Science Foundation of China(11071162)Shanghai Municipal Natural Science Foundation (09ZR1413500)
文摘In this paper, we will introduce how to apply Green's function method to get the pointwise estimates for the solutions of Cauchy problem of nonlinear evolution equations with dissipative structure. First of all, we introduce the pointwise estimates of the time-asymptotic shape of the solutions of the isentropic Navier-Stokes equations and show to exhibit the generalized Huygen's principle. Then, for other nonlinear dissipative evolution equations, we will only introduce the result and give some brief explanations. Our approach is based on the detailed analysis of the Green's function of the linearized system and micro-local analysis, such as frequency decomposition and so on.
基金National Natural Science Foundation of China Key Project,under Grant No.50538030Postdoctoral Science Foundation of China under Grant No.2013M531084Natural Science Foundation of Heilongjiang Province of China under Grant No.E201221
文摘This study proposes a Green's function, an essential representation of water-saturated ground under moving excitation, to simulate ground borne vibration from trains. First, general solutions to the governing equations of poroelastic medium are derived by means of integral transform. Secondly, the transmission and reflection matrix approach is used to formulate the relationship between displacement and stress of the stratified ground, which results in the matrix of the Green's function. Then the Green's function is combined into a train-track-ground model, and is verified by typical examples and a field test. Additional simulations show that the computed ground vibration attenuates faster in the immediate vicinity of the track than in the surrounding area. The wavelength of wheel-rail unevenness has a notable effect on computed displacement and pore pressure. The variation of vibration intensity with the depth of ground is significantly influenced by the layering of the strata soil. When the train speed is equal to the velocity of the Rayleigh wave, the Mach cone appears in the simulated wave field. The proposed Green's function is an appropriate representation for a layered ground with shallow ground water table, and will be helpful to understand the dynamic responses of the ground to complicated moving excitation.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest of China(201103005)the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(2013–2017)
文摘On the basis of a long-term(30 years) field experiment that involved four rotation systems, rice-rice-winter fallow(RRF), rice-rice-ryegrass(RRG), rice-rice-rape(RRP), and rice-rice-milk vetch(RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16 S r RNA gene. The Chao1 richness and non-parametric Shannon's index increased in all soil samples that received green manure treatments. The communities' structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.