《纺车旁的玛格丽特》(Gretchen am Spinnrade)又译为《纺车旁的格丽卿》《纺纱的格雷亨特》,是奥地利著名作曲家舒伯特的代表作之一。歌曲内容取材于德国伟大诗人歌德的《浮士德》第一部第十八场,塑造了一位沉湎于失恋悲伤中的少女形...《纺车旁的玛格丽特》(Gretchen am Spinnrade)又译为《纺车旁的格丽卿》《纺纱的格雷亨特》,是奥地利著名作曲家舒伯特的代表作之一。歌曲内容取材于德国伟大诗人歌德的《浮士德》第一部第十八场,塑造了一位沉湎于失恋悲伤中的少女形象。通过对该作品调性色彩、和声架构、伴奏织体以及术语标记的分析,并结合作曲家个人的创作风格及该作品的歌词内容,系统阐述了该作品钢琴伴奏的演奏技巧以及艺术指导与演唱者的配合技巧。展开更多
Anchorene,identified as an endogenous bioactive carotenoid-derived dialdehyde and diapocarotenoid,affects root development by modulating auxin homeostasis.However,the precise interaction between anchorene and auxin,as...Anchorene,identified as an endogenous bioactive carotenoid-derived dialdehyde and diapocarotenoid,affects root development by modulating auxin homeostasis.However,the precise interaction between anchorene and auxin,as well as the mechanisms by which anchorene modulates auxin levels,remain largely elusive.In this study,we conducted a comparative analysis of anchorene's bioactivities alongside auxin and observed that anchorene induces multifaceted auxin-like effects.Through genetic and pharmacological examinations,we revealed that anchorene's auxin-like activities depend on the indole-3-pyruvate-dependent auxin biosynthesis pathway,as well as the auxin inactivation pathway mediated by Group II Gretchen Hagen 3(GH3)proteins that mainly facilitate the conjugation of indole-3-acetic acid(IAA)to amino acids,leading to the formation of inactivated storage forms.Our measurements indicated that anchorene treatment elevates IAA levels while reducing the quantities of inactivated IAA–amino acid conjugates and ox IAA.RNA sequencing further revealed that anchorene triggers the expression of numerous auxin-responsive genes in a manner reliant on Group II GH3s.Additionally,our in vitro enzymatic assays and biolayer interferometry(BLI)assay demonstrated anchorene's robust suppression of GH3.17-mediated IAA conjugation with glutamate.Collectively,our findings highlight the significant role of carotenoid-derived metabolite anchorene in modulating auxin homeostasis,primarily through the repression of GH3-mediated IAA conjugation and inactivation pathways,offering novel insights into the regulatory mechanisms of plant bioactive apocarotenoids.展开更多
文摘《纺车旁的玛格丽特》(Gretchen am Spinnrade)又译为《纺车旁的格丽卿》《纺纱的格雷亨特》,是奥地利著名作曲家舒伯特的代表作之一。歌曲内容取材于德国伟大诗人歌德的《浮士德》第一部第十八场,塑造了一位沉湎于失恋悲伤中的少女形象。通过对该作品调性色彩、和声架构、伴奏织体以及术语标记的分析,并结合作曲家个人的创作风格及该作品的歌词内容,系统阐述了该作品钢琴伴奏的演奏技巧以及艺术指导与演唱者的配合技巧。
基金supported by the National Natural Science Foundation of China(32170271)by the Natural Science Foundation of Henan Province(222300420024)the Project of Sanya Yazhou Bay Science and Technology City(SCKJ-JYRC-2022-19)。
文摘Anchorene,identified as an endogenous bioactive carotenoid-derived dialdehyde and diapocarotenoid,affects root development by modulating auxin homeostasis.However,the precise interaction between anchorene and auxin,as well as the mechanisms by which anchorene modulates auxin levels,remain largely elusive.In this study,we conducted a comparative analysis of anchorene's bioactivities alongside auxin and observed that anchorene induces multifaceted auxin-like effects.Through genetic and pharmacological examinations,we revealed that anchorene's auxin-like activities depend on the indole-3-pyruvate-dependent auxin biosynthesis pathway,as well as the auxin inactivation pathway mediated by Group II Gretchen Hagen 3(GH3)proteins that mainly facilitate the conjugation of indole-3-acetic acid(IAA)to amino acids,leading to the formation of inactivated storage forms.Our measurements indicated that anchorene treatment elevates IAA levels while reducing the quantities of inactivated IAA–amino acid conjugates and ox IAA.RNA sequencing further revealed that anchorene triggers the expression of numerous auxin-responsive genes in a manner reliant on Group II GH3s.Additionally,our in vitro enzymatic assays and biolayer interferometry(BLI)assay demonstrated anchorene's robust suppression of GH3.17-mediated IAA conjugation with glutamate.Collectively,our findings highlight the significant role of carotenoid-derived metabolite anchorene in modulating auxin homeostasis,primarily through the repression of GH3-mediated IAA conjugation and inactivation pathways,offering novel insights into the regulatory mechanisms of plant bioactive apocarotenoids.
