Grifola frondosa polysaccharide(GFP)as the natural compounds have been reported to exert diverse bioactivities.The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 ...Grifola frondosa polysaccharide(GFP)as the natural compounds have been reported to exert diverse bioactivities.The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice.The changes of microbiota in faeces of the diabetic mice upon high fat diet were determined and the V3 region of the 16S rRNA was sequenced by Illumina MiSeq high-throughput sequencing platform.Eighty operational taxonomic unit were identified to be shared by all samples,and the quality and richness of sequencing were assessed via rarefaction and rank abundance curves.The diversity of gut flora was slightly improved in diabetic mice after GFP treatment.The composition and relative abundance of gut microbiota at phylum and genus levels were altered by GFP.The abundance of Robinsoniella,Flavonifractor,Anaerotruncus,and Desulfvibrio were found to concentrate on diabetic mice through LEfSe analysis.Furthermore,Alloprevotella and Burkholderia had strong relevancy with other gut flora.Based on the findings,GFP might be a desired candidate on ameliorating the intestinal unbalance in diabetes.展开更多
The Box-Behnken design was used to determine the optimal time,temperature and solvent∶solid ratio for extracting intracellular polysaccharide from G.frondosa mycelium produced under submerged culture conditions.Appli...The Box-Behnken design was used to determine the optimal time,temperature and solvent∶solid ratio for extracting intracellular polysaccharide from G.frondosa mycelium produced under submerged culture conditions.Application of response surface methodology predicted a maximum polysaccharide extraction yield of 7.28% using an extraction time of 2.24 h,an extraction temperature of 92.53 ℃,and a water∶mycelium ratio of 35.76∶1.Under these conditions,the actual yield was 7.15%.Both low(200 mg/kg/d)and high(500 mg/kg/d)doses of G.frondosa polysaccharide,and positive controls administered 20 mg/kg/d cyclophosphamide,significantly inhibited the growth of S180 tumors with inhibition values of 61.5%,44.42% and 73.8%,respectively compared with negative controls administered physiological saline.展开更多
利用DEAE-Sepharose Fast Flow离子柱层析和Sephacryl凝胶柱层析对灰树花(Grifola frondosa)子实体水提醇沉粗多糖进行分离纯化,对分离纯化到的一种多糖GFP75-2-2B进行了分析。紫外光谱鉴定结果表明,GFP75-2-2B不含蛋白质、核酸和酚类成...利用DEAE-Sepharose Fast Flow离子柱层析和Sephacryl凝胶柱层析对灰树花(Grifola frondosa)子实体水提醇沉粗多糖进行分离纯化,对分离纯化到的一种多糖GFP75-2-2B进行了分析。紫外光谱鉴定结果表明,GFP75-2-2B不含蛋白质、核酸和酚类成分;离子色谱分析表明其单糖组成为岩藻糖、鼠李糖、半乳糖、葡萄糖和甘露糖,摩尔比为1.0∶1.3∶3.6∶4.0∶4.5。GFP75-2-2B对巨噬细胞株RAW264.7进行体外免疫活性的研究结果表明,GFP75-2-2B有明显刺激巨噬细胞分泌NO的活性。展开更多
The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa(GFP) by using mouse peritoneal macrophage and cytoxan(CTX) induced immunosuppression models. Our res...The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa(GFP) by using mouse peritoneal macrophage and cytoxan(CTX) induced immunosuppression models. Our results from the phagocytotic and mononuclear phagocytic system function assays showed that GFP-A(one component from GFP) stimulated the phagocytosis of the phagocytes. The splenocyte proliferation assay showed that GFP-A acted the effect combing Con A or LPS in splenocyte proliferation. The results showed that GFP-A increased indices of thymus and spleen, the levels of LDH and ACP in the spleen, the m RNA levels of IL-1β, IL-2, IL-6 and IFN-γ in splenocyte. And GFP-A also significantly increased the expression of CD4^+ and CD8^+ splenic T lymphocytes, which were suppressed by the CTX in peripheral blood. In conclusion, our results indicate that the GFP-A is involved in immunomodulatory effects leading to its modulatory effects on immunosuppression.展开更多
基金financially supported by Double First-Class Construction Plan(KSYLX013)Science and Technology Innovation Project(CXZX2017524)of Fujian Agriculture and Forestry University.
文摘Grifola frondosa polysaccharide(GFP)as the natural compounds have been reported to exert diverse bioactivities.The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice.The changes of microbiota in faeces of the diabetic mice upon high fat diet were determined and the V3 region of the 16S rRNA was sequenced by Illumina MiSeq high-throughput sequencing platform.Eighty operational taxonomic unit were identified to be shared by all samples,and the quality and richness of sequencing were assessed via rarefaction and rank abundance curves.The diversity of gut flora was slightly improved in diabetic mice after GFP treatment.The composition and relative abundance of gut microbiota at phylum and genus levels were altered by GFP.The abundance of Robinsoniella,Flavonifractor,Anaerotruncus,and Desulfvibrio were found to concentrate on diabetic mice through LEfSe analysis.Furthermore,Alloprevotella and Burkholderia had strong relevancy with other gut flora.Based on the findings,GFP might be a desired candidate on ameliorating the intestinal unbalance in diabetes.
基金Sponsored by the Government of Shandong Province(No.2007LZ07)
文摘The Box-Behnken design was used to determine the optimal time,temperature and solvent∶solid ratio for extracting intracellular polysaccharide from G.frondosa mycelium produced under submerged culture conditions.Application of response surface methodology predicted a maximum polysaccharide extraction yield of 7.28% using an extraction time of 2.24 h,an extraction temperature of 92.53 ℃,and a water∶mycelium ratio of 35.76∶1.Under these conditions,the actual yield was 7.15%.Both low(200 mg/kg/d)and high(500 mg/kg/d)doses of G.frondosa polysaccharide,and positive controls administered 20 mg/kg/d cyclophosphamide,significantly inhibited the growth of S180 tumors with inhibition values of 61.5%,44.42% and 73.8%,respectively compared with negative controls administered physiological saline.
文摘利用DEAE-Sepharose Fast Flow离子柱层析和Sephacryl凝胶柱层析对灰树花(Grifola frondosa)子实体水提醇沉粗多糖进行分离纯化,对分离纯化到的一种多糖GFP75-2-2B进行了分析。紫外光谱鉴定结果表明,GFP75-2-2B不含蛋白质、核酸和酚类成分;离子色谱分析表明其单糖组成为岩藻糖、鼠李糖、半乳糖、葡萄糖和甘露糖,摩尔比为1.0∶1.3∶3.6∶4.0∶4.5。GFP75-2-2B对巨噬细胞株RAW264.7进行体外免疫活性的研究结果表明,GFP75-2-2B有明显刺激巨噬细胞分泌NO的活性。
基金supported by Key Projects in National Science&Technology Pillar Program during the 12th Five-Year Plan Period(No.2012BAD33B04)Quality of Commodity Grain and Oil Resources and Processing Database Research–Grains(No.201313006)+5 种基金Key Projects of Tianjin Natural Science Foundation(No.13JCZDJC29800)863 National Plan(No.2012 AA022108)Agricultural Science and Technology Transformation Fund(No.2012GB2A100016)Plan for the Development of Changjiang Scholars and Innovative Research Team(No.IRT1166)National Nature Science Foundation of China(Nos.31000768 and 31171731)Tianjin Science and Technology Support Plan(No.10ZCZDSY 07000)
文摘The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa(GFP) by using mouse peritoneal macrophage and cytoxan(CTX) induced immunosuppression models. Our results from the phagocytotic and mononuclear phagocytic system function assays showed that GFP-A(one component from GFP) stimulated the phagocytosis of the phagocytes. The splenocyte proliferation assay showed that GFP-A acted the effect combing Con A or LPS in splenocyte proliferation. The results showed that GFP-A increased indices of thymus and spleen, the levels of LDH and ACP in the spleen, the m RNA levels of IL-1β, IL-2, IL-6 and IFN-γ in splenocyte. And GFP-A also significantly increased the expression of CD4^+ and CD8^+ splenic T lymphocytes, which were suppressed by the CTX in peripheral blood. In conclusion, our results indicate that the GFP-A is involved in immunomodulatory effects leading to its modulatory effects on immunosuppression.
