The experiment of tissue culture of ground-cover chrysanthemum with the tip of shoots as the explant was made. On MS medium, with adding BA 3.0 mg/ L and NAA 0.01mg / L, the adventitious buds had the best multip1icati...The experiment of tissue culture of ground-cover chrysanthemum with the tip of shoots as the explant was made. On MS medium, with adding BA 3.0 mg/ L and NAA 0.01mg / L, the adventitious buds had the best multip1ication result. The best result for taking roots was obtained on 1 / 2 MS medium with adding NAA 0.1 mg / L. It had good result and a high survival rate for transplanting the young test-tube plants into soil whcn they were 3-4 cm high,with 5-7 rootlets (0.5- 1.0 cm in length). 30% of edible powdered sugar was uscd to replace sucrose in order to lower thc cost and the same results were obtained.展开更多
[Objectives]Among many chrysanthemum types,the ground-cover chrysanthemum cultivar group has the characteristics of dwarf plant,long flowering period,rich color and strong resistance to stress.This study aimed to scre...[Objectives]Among many chrysanthemum types,the ground-cover chrysanthemum cultivar group has the characteristics of dwarf plant,long flowering period,rich color and strong resistance to stress.This study aimed to screen out cultivars with strong cold tolerance in order that they can be used for planting in urban green space and economic forests in northern cold areas,so as to enrich the plant community structures of landscape in cold regions.[Methods]Three ground-cover chrysanthemum cultivars,i.e.,‘Meiaihuang’,‘Xinhong’and‘Zixunzhang’,were selected as the experimental materials.During the wintering period in the field in Beijing,they were observed and measured for the changes of the morphological manifestations and the physiological indices,and the relationship between the indices and low temperature was analyzed.The cold tolerance of the three ground-cover chrysanthemum cultivars was also comprehensively evaluated.[Results]During the open-field overwintering period,the root activity of the three ground-cover chrysanthemum cultivars increased slowly with cooling,decreased at the freezing low temperature,and then increased again in early spring.With natural cooling,the membrane injury percentages(MIPs)of the leaves and foot shoots increased significantly,and the contents of malondialdehyde(MDA)increased;the contents of soluble sugar(SS)and proline(Pro)gradually accumulated,and the contents of SS and Pro in foot shoots increased significantly in late winter;and the superoxide dismutase(SOD)activity showed a similar trend of rising slowly at first and then increasing significantly.There were correlations between MIPs in the leaves and foot shoots of the three cultivars and other physiological indices:a significant negative correlation with MDA content,and a significant positive correlation with SS content,Pro content and SOD activity.[Conclusions]Combining the morphological manifestations and physiological indices,the cold tolerance of the three ground-cover chrysanthemum cultivars was evaluated by the subordinate function method.The results showed that the cold tolerance of the three ground-cover chrysanthemum cultivars ranked as‘Meiaihuang’>‘Xinhong’>‘Zixunzhang’.The strong cold tolerance of foot shoots and roots was the main reason that the three ground-cover chrysanthemum cultivars could overwinter in the open field.展开更多
文摘The experiment of tissue culture of ground-cover chrysanthemum with the tip of shoots as the explant was made. On MS medium, with adding BA 3.0 mg/ L and NAA 0.01mg / L, the adventitious buds had the best multip1ication result. The best result for taking roots was obtained on 1 / 2 MS medium with adding NAA 0.1 mg / L. It had good result and a high survival rate for transplanting the young test-tube plants into soil whcn they were 3-4 cm high,with 5-7 rootlets (0.5- 1.0 cm in length). 30% of edible powdered sugar was uscd to replace sucrose in order to lower thc cost and the same results were obtained.
基金Supported by Science&Technology Nova Project under Beijing Science and Technology Commission(No.9558101600)Project of Construction of Advanced Horticulture under Beijing Municipality(2020)"Opening the List and Taking Charge"Project under Beijing University of Agriculture(2021).
文摘[Objectives]Among many chrysanthemum types,the ground-cover chrysanthemum cultivar group has the characteristics of dwarf plant,long flowering period,rich color and strong resistance to stress.This study aimed to screen out cultivars with strong cold tolerance in order that they can be used for planting in urban green space and economic forests in northern cold areas,so as to enrich the plant community structures of landscape in cold regions.[Methods]Three ground-cover chrysanthemum cultivars,i.e.,‘Meiaihuang’,‘Xinhong’and‘Zixunzhang’,were selected as the experimental materials.During the wintering period in the field in Beijing,they were observed and measured for the changes of the morphological manifestations and the physiological indices,and the relationship between the indices and low temperature was analyzed.The cold tolerance of the three ground-cover chrysanthemum cultivars was also comprehensively evaluated.[Results]During the open-field overwintering period,the root activity of the three ground-cover chrysanthemum cultivars increased slowly with cooling,decreased at the freezing low temperature,and then increased again in early spring.With natural cooling,the membrane injury percentages(MIPs)of the leaves and foot shoots increased significantly,and the contents of malondialdehyde(MDA)increased;the contents of soluble sugar(SS)and proline(Pro)gradually accumulated,and the contents of SS and Pro in foot shoots increased significantly in late winter;and the superoxide dismutase(SOD)activity showed a similar trend of rising slowly at first and then increasing significantly.There were correlations between MIPs in the leaves and foot shoots of the three cultivars and other physiological indices:a significant negative correlation with MDA content,and a significant positive correlation with SS content,Pro content and SOD activity.[Conclusions]Combining the morphological manifestations and physiological indices,the cold tolerance of the three ground-cover chrysanthemum cultivars was evaluated by the subordinate function method.The results showed that the cold tolerance of the three ground-cover chrysanthemum cultivars ranked as‘Meiaihuang’>‘Xinhong’>‘Zixunzhang’.The strong cold tolerance of foot shoots and roots was the main reason that the three ground-cover chrysanthemum cultivars could overwinter in the open field.
