In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-bindi...In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-binding properties of the selected phage peptides were evaluated by phage ELISA and phage capture assay. The peptides were co-expressed as glutathione S-transferase （GST） fusion proteins. RBC agglutination inhibition assay was performed to assess the natural blood group A antigen-mimicking ability of the fusion proteins. The results showed that seven phage clones selected bound to NaM87-1F6 specifically, among which, 6 clones bore the same peptide sequence, EYWYCGMNRTGC and another harbored a different one QIWYERTLPFTF. The two peptides were successfully expressed at the N terminal of GST protein. Both of the fusion proteins inhibited the RBC agglutination mediated by anti-A serum in a concentration-dependent manner. These results suggested that the fusion proteins based on the selected peptides could mimic the blood group A antigen and might be used as anti-A antibody-adsorbing materials when immunoabsorption was applied in ABO incompatible transplantation.展开更多
[目的]本文旨在分析花色测试常用工具——英国皇家园艺学会比色卡(Royal Horticultural Society Color Chart,RHSCC)长期使用后的颜色变化情况,以及颜色变化在花色测定和花色性状品种分组上的影响。[方法]使用色差仪对新(未使用)和旧(使...[目的]本文旨在分析花色测试常用工具——英国皇家园艺学会比色卡(Royal Horticultural Society Color Chart,RHSCC)长期使用后的颜色变化情况,以及颜色变化在花色测定和花色性状品种分组上的影响。[方法]使用色差仪对新(未使用)和旧(使用10年)的RHSCC进行颜色测定,并利用色差仪及新、旧2套RHSCC对150个长寿花品种进行花色测定和花色分组,分析比较3种测色分组结果的优劣。[结果]新、旧2套RHSCC在统计学水平上存在显著差异(P<0.001),差异大小与色块使用频率和装订孔距离成正相关;884个色块中除1个色块外,2套RHSCC色差值小于人眼可觉察的1.5个CIE LAB单位;2套RHSCC在长寿花花色测定上一致性为100%。另外,基于RHSCC和目测的品种分组存在边界不清,部分分组过大、过小或重叠等问题。基于CIE LAB和聚类分析的品种分组将150个品种划分为白、黄、橙、浅粉、粉、红和紫红7大色组,其品种花色分组的合理性在花色测定值L*a*b*三维色空间、a*b*二维坐标分布、箱线图、视觉图像中得以证实。[结论]新、旧2套RHSCC色卡间的颜色变化统计学差异在人眼可察觉范围外,不影响目测比色法的花色测定结果,但RHSCC目测比色的品种花色分组间存在重叠区间,不宜应用于严格的品种花色分组。展开更多
文摘In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-binding properties of the selected phage peptides were evaluated by phage ELISA and phage capture assay. The peptides were co-expressed as glutathione S-transferase （GST） fusion proteins. RBC agglutination inhibition assay was performed to assess the natural blood group A antigen-mimicking ability of the fusion proteins. The results showed that seven phage clones selected bound to NaM87-1F6 specifically, among which, 6 clones bore the same peptide sequence, EYWYCGMNRTGC and another harbored a different one QIWYERTLPFTF. The two peptides were successfully expressed at the N terminal of GST protein. Both of the fusion proteins inhibited the RBC agglutination mediated by anti-A serum in a concentration-dependent manner. These results suggested that the fusion proteins based on the selected peptides could mimic the blood group A antigen and might be used as anti-A antibody-adsorbing materials when immunoabsorption was applied in ABO incompatible transplantation.
文摘[目的]本文旨在分析花色测试常用工具——英国皇家园艺学会比色卡(Royal Horticultural Society Color Chart,RHSCC)长期使用后的颜色变化情况,以及颜色变化在花色测定和花色性状品种分组上的影响。[方法]使用色差仪对新(未使用)和旧(使用10年)的RHSCC进行颜色测定,并利用色差仪及新、旧2套RHSCC对150个长寿花品种进行花色测定和花色分组,分析比较3种测色分组结果的优劣。[结果]新、旧2套RHSCC在统计学水平上存在显著差异(P<0.001),差异大小与色块使用频率和装订孔距离成正相关;884个色块中除1个色块外,2套RHSCC色差值小于人眼可觉察的1.5个CIE LAB单位;2套RHSCC在长寿花花色测定上一致性为100%。另外,基于RHSCC和目测的品种分组存在边界不清,部分分组过大、过小或重叠等问题。基于CIE LAB和聚类分析的品种分组将150个品种划分为白、黄、橙、浅粉、粉、红和紫红7大色组,其品种花色分组的合理性在花色测定值L*a*b*三维色空间、a*b*二维坐标分布、箱线图、视觉图像中得以证实。[结论]新、旧2套RHSCC色卡间的颜色变化统计学差异在人眼可察觉范围外,不影响目测比色法的花色测定结果,但RHSCC目测比色的品种花色分组间存在重叠区间,不宜应用于严格的品种花色分组。
