Difference in the anti-fatigue effect of moxibustion at different acupoints in one-off exhausted rats

Objective:To explore the difference in the anti-fatigue effect of moxibustion at different acupoints through observing the impacts of moxibustion at"Shènshù(肾俞BL23)","Zúsānlǐ(足三里ST36)","Guānyuán(关元CV4)"and"Shénquè(神阙CV8)"on energy metabolic indexes in the one-off exhausted rats.Methods:A total of 56 SPF male rats were randomized into 7 groups,named a blank group,a model group,a non-acupoint group,a"BL23"group,a"ST36"group,a"CV4"group and a"CV8"group,8 rats in each group.The one-off exhaustion model was established by weight-bearing swimming experiment.Right after the exhaustive swimming of rats in each intervention group,the mild moxibustion was applied at the corresponding acupoints for 15 min immediately.4 h after the exhaustive swimming,the blood was collected from the femoral artery and the quadriceps femoris of the left hindlimb was stripped.The levels of blood lactate(BLD),lactate dehydrogenase(LDH),creatine kinase(CK)and blood urea nitrogen(BUN),the expressions of adenosine triphosphatease(ATP),fructose-6-phosphofructokinase(PFK)and malomdialdehyde(MDA)in the quadriceps femoris and the activity of superoxide dismutase(SOD)and catalase(CAT)were detected.Results:Compared with the model group and the non-acupoint group,the levels of serum BLD,LDH,CK and BUN were all lower those in the"BL23"group,the"ST36"group,the"CV4"group and the"CV8"group,indicating a statistical significance(all P<0.05).Compared with the model group and the nonacupoint group,PFK was higher in the"BL23"group,indicating a statistical significance(both P<0.05).Compared with the model group and the non-acupoint group,PFK was higher in the"ST36"group and the"CV4"group,indicating a statistical significance(all P<0.05).Conclusion:It was found in the research that moxibustion at"BL23""ST36""CV4"and"CV8"regulated the energy metabolism in the one-off exhausted rats so as to bring the anti-fatigue effect into play and the effect was different among the acupoints.Moxibustion at"CV8"may work on the prevention of the protein decomposition in the skeletal muscle and moxibustion at"BL23"may be against the oxidation in the skeletal muscle.

保健灸关元穴对老年大鼠血清和肠道氧化及炎症反应的影响

目的观察保健灸关元穴对老年大鼠血清和肠道氧化及炎症反应的影响,探讨其延缓衰老的可能机制。方法按照随机数字表法将12只正常老化雌性Wistar大鼠分为老年组(n=6)和保健灸组(n=6),选取6只青年雌性Wistar大鼠为青年组(n=6)。将三组大鼠腹部关元穴穴区直径约1.0 cm的鼠毛剃净。青年组和老年组:抓取大鼠放入特制固定器中,不施加其他干预手段,20 min/d,连续40 d;保健灸组:抓取大鼠放进特制固定器中,使其腹部对准固定器底部的开口,开口下方放置点燃的艾条,对准大鼠关元穴,及时调整艾条与大鼠的距离,使艾灸时的温度基本保持恒定,艾灸干预20 min/d,连续40 d。检测大鼠外周血清和肠道超氧化物歧化酶(SOD)、丙二醛(MDA)及肠道炎症因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-10(IL-10)水平,采用激光多普勒血流仪检测关元穴微循环血流灌注量。结果与青年组比较,老年组血清及肠道SOD水平降低(P<0.05或P<0.01),MDA水平升高(P<0.05或P<0.01);与老年组比较,保健灸组肠道SOD水平明显升高(P<0.01),MDA水平明显降低(P<0.01)。与青年组比较,老年组肠道IL-6、TNF-α和IL-10表达水平升高(P<0.05);与老年组比较,保健灸组肠道IL-6、TNF-α、IL-1β、IL-10表达水平显著降低(P<0.01)。与青年组比较,老年组关元穴微循环波峰降低,波幅变小,血流灌注量大幅下降;与老年组比较,保健灸组关元穴微循环波峰上升,波幅变大,血流灌注量明显上升。结论艾灸关元穴可以调节肠道免疫系统,提升自然衰老型大鼠抗氧化能力,其机制可能与降低血清和肠道炎症细胞因子水平、增强SOD清除体内氧自由基的能力、提高穴区微循环血流量有关。

针刺关元对寒凝证类痛经大鼠三阴交穴区温度及TXB_2/6-keto-PGF_(1a)比值的影响

目的观察针刺关元穴对寒凝证类痛经大鼠三阴交穴区温度(双侧)的影响并探讨其与TXB_2/6-keto-PGF_(1a)有关的机制。方法将大鼠随机分为盐水组、寒凝证类痛经模型组(简称模型组)、针刺关元组。采用全身冷冻法结合苯甲酸雌二醇和缩宫素注射法造模,用红外热成像方法检测各组大鼠左右两侧三阴交穴区0、5、10、20、30、40、50、60 min各时间点温度,同时用ELISA法测定血浆TXB_2和6-keto-PGF_(1a)含量。结果盐水组在基础温度、0~60 min各个时间点,大鼠左右三阴交穴区温度平衡(P>0.05);模型组在5~60 min各个时间点,大鼠左右三阴交穴区温度失衡(P<0.05,P<0.01);针刺关元组在基础温度、0~30 min大鼠左右三阴交穴区温度失衡(P<0.05),40~60 min逐渐趋近平衡(P>0.05)。与盐水组比较,模型组TXB_2/6-keto-PGF_(1a)比值明显升高(P<0.05);与模型组比较,针刺关元组TXB_2/6-keto-PGF_(1a)比值明显降低(P<0.05)。结论寒凝证类痛经大鼠左右三阴交穴区温度失衡;针刺关元穴能调节其失衡状态,且调节需要一定的时间过程,在针刺结束后10~40 min作用显著,其内在机制与针刺调节体内血管舒缩物质TXB_2/6-ketoPGF_(1a)比值的失衡有关。