期刊文献+
共找到62篇文章
< 1 2 4 >
每页显示 20 50 100
转基因水稻中GUS蛋白质的检测及其表达特征 被引量:10
1
作者 牛东东 郝育杰 +13 位作者 荣瑞娟 韦汉福 兰金苹 史佳楠 魏健 李雪姣 杨烁 奚文辉 武鹏程 刘丽娟 吴琳 刘斯奇 尹长城 刘国振 《中国农业科学》 CAS CSCD 北大核心 2014年第14期2715-2722,共8页
【目的】建立转基因水稻中GUS蛋白质的免疫学检测方法,并了解花椰菜花叶病毒(CaMV)35S启动子驱动的GUS蛋白质在转基因水稻中的表达特征。【方法】以细菌基因组DNA为模板,PCR扩增GUS基因后克隆到表达载体pET30a中,测序验证的重组子转入... 【目的】建立转基因水稻中GUS蛋白质的免疫学检测方法,并了解花椰菜花叶病毒(CaMV)35S启动子驱动的GUS蛋白质在转基因水稻中的表达特征。【方法】以细菌基因组DNA为模板,PCR扩增GUS基因后克隆到表达载体pET30a中,测序验证的重组子转入大肠杆菌表达菌BL21中,IPTG诱导获得重组表达的GUS蛋白质,用HIS-tag beads纯化后作为免疫原免疫小鼠制备GUS蛋白质特异的抗体,通过免疫印迹分析筛选高特异性的单克隆抗体,用Broadford法对重组的GUS蛋白质进行定量,对不同浓度的GUS蛋白质进行免疫印迹分析,绘制检测GUS蛋白质的标准曲线,通过与标准曲线的比较对水稻叶片中GUS蛋白质进行定量分析。提取不同时期、不同部位的水稻总蛋白质,包括苗期的地上部、地下部,分蘖期的茎、茎节、叶鞘、叶枕、叶片上部、叶片中部和叶片下部,孕穗期的茎、穗轴、叶鞘、叶枕、叶片、幼穗(长度分别为1、2、10和20 cm),开花期的茎、穗轴、叶鞘、叶片、穗子,成熟期的茎、叶片、授粉后不同时期的种子(分别为授粉后10、20、30和40 d)、乳熟期的胚、胚乳和颖壳、成熟种子的全种子、胚、胚乳和颖壳以及不同时期的叶片和根部材料等。SDS-PAGE分离后用抗体检测其GUS蛋白质的丰度。【结果】筛选获得了高特异性的抗GUS单克隆抗体(编号为#27),用该抗体检测转基因水稻中及重组的GUS蛋白质均呈现特异条带,没有可见的背景信号,用本研究建立的免疫印迹方法对重组GUS蛋白质的检测下限约为4 ng,可检出转基因水稻单粒大米2.5%样品中(约0.6 mg)的GUS蛋白质。在不同时期的转基因水稻叶片中GUS蛋白质的表达丰度基本稳定,而在水稻根部的GUS丰度随生长急剧减少,5叶期根中的表达量不到3叶期的三分之一,到6叶期检测不到GUS蛋白质。在水稻苗期叶片中,GUS蛋白质约占鲜重的0.02‰。另外,除分蘖期以后的根部之外,GUS蛋白质几乎在所有的水稻组织部位中呈组成型表达,只是不同组织中的表达量略有差异,如在孕穗期和开花期的茎及颖壳中的表达量较低。【结论】建立了具有应用价值的对转基因水稻中GUS蛋白质丰度检测的免疫印记方法。该方法特异性高、样品用量少、不依赖于GUS蛋白质的酶活性、测定结果易于在不同实验室间比较。证明了35S启动子驱动的GUS蛋白质在转基因水稻中基本呈组成型表达。 展开更多
关键词 水稻 转基因植物 CaMV 35s启动子 gus蛋白质 免疫印迹
下载PDF
CaMV 35S启动子在菊花中驱动GUS外源基因的表达分析 被引量:3
2
作者 阳淑金 宋爱萍 +6 位作者 何深颖 朱晓晨 孙静 高姣姣 王银杰 陈发棣 蒋甲福 《南京农业大学学报》 CAS CSCD 北大核心 2015年第4期554-559,共6页
[目的]由于CaMV 35S启动子转化菊花存在外源基因表达量低、基因沉默等问题,本研究对35S启动子、2×35S启动子在菊花中驱动GUS外源基因的表达进行了分析,以期为高效菊花转化提供理论指导。[方法]通过设计引物,利用PCR从p CAMBIA1301 ... [目的]由于CaMV 35S启动子转化菊花存在外源基因表达量低、基因沉默等问题,本研究对35S启动子、2×35S启动子在菊花中驱动GUS外源基因的表达进行了分析,以期为高效菊花转化提供理论指导。[方法]通过设计引物,利用PCR从p CAMBIA1301 Vector中克隆了35S启动子、2×35S启动子的序列并插入pORE R2载体中,分别获得了重组载体p ORE R2-35S:GUS和p ORE R2-2×35S:GUS,通过农杆菌介导法将构建的遗传转化载体分别导入切花菊品种‘神马’中。[结果]试验共转化2 296个菊花叶盘,经过筛选共得到16个过量表达的转基因株系。经PCR验证,目标片段已经成功插入转基因株系基因组。荧光定量PCR分析和染色分析显示,在转基因菊花中2×35S比35S启动子表现出驱动GUS基因高量表达的特性。[结论]在选择合适菊花品种的基础上,2×35S比35S启动子可以更加高效地促进基因表达。 展开更多
关键词 菊花 转基因 35s启动子 gus表达
下载PDF
GU'S FUNCTION SCALE OF ION HYDRATION FORCE AND CHARGE SIZE
3
作者 顾宏堪 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 1997年第2期186-191,共6页
Water Vapor Chemistry" as a new science was discovered and established from trace analysis in Gu’s laboratory(Gu, 1991; Gu et al., 1991). Gu’s Function shows that the trace metal ion concentration in water vapo... Water Vapor Chemistry" as a new science was discovered and established from trace analysis in Gu’s laboratory(Gu, 1991; Gu et al., 1991). Gu’s Function shows that the trace metal ion concentration in water vapor has positive correlation to"specific electron affinity constant"last ionization potential Iz/ion valence Z and negative correlation to ion volume V and coordination number N(Gu, 1994). Gu’s Func-tion C=f[(Iz/Z)/VN]of the bond parameter in water vapor chemistry corresponds to the potential energy function Z’ e2/r in the Schrodinger Equation of quantum chemistry. In different ions with the same 2+ charge, the ion concentration of water-water vapor transfer may be much different. This shows that the 2+ charge of different ions has different attractive force (hydration force). This different attractive force of the charge can be scaled with the relative energy or charge size from Gu’s Function. 展开更多
关键词 water vapor chemistry gus function sCALE ION hydration FORCE ION CHARGE sIZE
下载PDF
Gardening Art of Gu Dongyang's Memorial Temple in Wuxi City,Jiangsu Province
4
作者 ZHU Yunfeng 《Journal of Landscape Research》 2013年第6期16-18,共3页
On the basis of explaining goals of building temple gardens,Gu Dongyang’s Memorial Temple in Wuxi City was taken for example to analyze its gardening arts from the perspective of water layout,stone layout,building an... On the basis of explaining goals of building temple gardens,Gu Dongyang’s Memorial Temple in Wuxi City was taken for example to analyze its gardening arts from the perspective of water layout,stone layout,building and plant design,and elaborate its cultural connotations from decorative symbols of its architectural components.It was concluded that Gu Dongyang’s Memorial Temple,in spite of its small size and plain style,had profound historical background and high referential value for furthering the study on temple gardens in the south of the Yangtze River. 展开更多
关键词 gu Dongyang’s MEMORIAL TEMPLE GARDEN GARDENING ART
下载PDF
Land Use Dynamics in the Department of Séguéla, Northwestern Côte D’Ivoire
5
作者 Arthur Brice Konan-Waidhet Kouakou Hervé Kouassi Kouamé Elyass Kanga 《Advances in Remote Sensing》 2022年第3期63-79,共17页
Land use monitoring occupies a very important place in the analysis of the dynamics of the earth system. It helps to understand the organization and helps to provide relevant elements for the establishment of diagnose... Land use monitoring occupies a very important place in the analysis of the dynamics of the earth system. It helps to understand the organization and helps to provide relevant elements for the establishment of diagnoses and the development of environmental forecasts. The objective of this study is to follow the evolution of the agricultural landscape in the department of Séguéla from 1988 to 2020 and to make a prediction for 2050, in order to manage the spaces reasonably. The methodology adopted is based on the one hand on the processing of satellite images for the analysis of land cover and on the other hand on predictive modeling (LCM model) by 2050. The results obtained show that the land use maps produced after processing the satellite images made it possible to highlight the dynamics of the agricultural landscape in this part of the Worodougou region. During the period 1988 to 2020, we witness an increase in the area of cultivated territory as well as a slight reduction in wooded savannas which are largely made up of perennial crops (cashew trees, cocoa trees, coffee trees, etc.). These two aforementioned classes have respective annual rates of change of 2.42% and &#8722;0.44%. A scenario modeling land cover changes in 2050 with an overall accuracy of 80.35% revealed a continued growth of crops and fallows to the detriment of natural forests and wooded savannas. 展开更多
关键词 satellite Images Land Use LCM Model Land Cover Change séguéla
下载PDF
Geochemical Signatures of Séguéla Peridotites in the West African Craton
6
作者 Allialy Marc Ephrem Dicko Kagou +2 位作者 Houssou N’guessan Nestor Kouamelan Alain Nicaise Coulibaly Yacouba 《Journal of Geoscience and Environment Protection》 2022年第12期100-116,共17页
The present study deals with peridotites found in the paleo-proterozoic domain of the Leo Man Shield, Séguéla region, west-central of C&#244;te d’Ivoire. Results show that Séguéla peridotites ... The present study deals with peridotites found in the paleo-proterozoic domain of the Leo Man Shield, Séguéla region, west-central of C&#244;te d’Ivoire. Results show that Séguéla peridotites are composed of lherzolites, dunites and harzburgites. However, iherzolites are the most abundant. The phenocrysts in these rocks are olivine most often serpentinised, and pyroxenes, represented by diopside and enstatite. Amphiboles are divided into two groups: magmatic amphiboles and those from the destabilization of clinopyroxene. Spinels have CrO<sub>3</sub> content which varies between 28% and 37%, Al<sub>2</sub>O<sub>3</sub> between 33% - 41% and MgO is equal to 18%;they are poor in TiO<sub>2</sub> and do not contain zinc oxide. Séguéla peridotites are characterised by a negative anomaly in Nb-Ta, associated with an enrichment of lithophilic elements and light rare earth elements with a fractionation rate (La/Yb) which varies between 46.22 and 150.72 and heavy rare earth elements, and HFSE depletion, which may suggest that were formed in an art context, that is, a mantle enriched by fluids from a subduction zone. However, another hypothesis could be considered, that of the interaction between the mantle and magmas in a subduction zone context. 展开更多
关键词 Geochemestry PERIDOTITEs LHERZOLITE West African Craton séguéla Côte d’Ivoire
下载PDF
Ms Gu Ze's Art Collection
7
《China Today》 2002年第9期66-67,共2页
IN the 1990s, art collecting began to be a popular pursuit in China. At that time a large number of middle-aged and young artists carried out artistic exploration, creating numerous works with new artistic concepts an... IN the 1990s, art collecting began to be a popular pursuit in China. At that time a large number of middle-aged and young artists carried out artistic exploration, creating numerous works with new artistic concepts and styles. Active artistic cre- 展开更多
关键词 Ms gu Ze’s Art Collection
下载PDF
Fouimba and Goma Mounts Greenstone Belts Litho-Structural Analysis Related to Côte D’Ivoire Birimian Geodynamic Setting and Implying in West-Africa Craton Gold Deposits
8
作者 Marc Ephrem Allialy Brice Roland Kouassi +3 位作者 N’guessan Nestor Houssou Fossou Jean Luc Kouadio Davy Bedel Siogbo Félix Oufouet Konan 《Journal of Geoscience and Environment Protection》 2023年第1期150-168,共19页
Mount Fouimba and Mount Goma (Seguela) greenstone belts petro-structural studies combine remote sensing, geophysics, petrography and structural analysis. In view of establishing mapping details of paleoproterozoic geo... Mount Fouimba and Mount Goma (Seguela) greenstone belts petro-structural studies combine remote sensing, geophysics, petrography and structural analysis. In view of establishing mapping details of paleoproterozoic geological formations, geological setting rocks observed are essentially magmatic formations, such as two-mica granite, granodiorites, and porphyritic basalts;and a few metamorphics which are metatonalite, amphibolites and amphibo-lopyroxenites. Remote sensing, such as Landsat 8 OLI satellite imagery and geophysical data, has been combined to show regional NNE-SW shear zone. Tectonic structures and microstructures have enabled to identify two main deformation phases: D1 phase corresponding to compression, and D2 is a transpression phase. Mechanisms responsible for deformations are respectively flattening and transpression. Geological formations derived from mantle origin but contain crustal components, and their tectonic setting occurred during subduction. 展开更多
关键词 Petro-structural Mapping Greenstone Belt BIRIMIAN séguéla Côte D’Ivoire
下载PDF
花椰菜花叶病毒(CaMV)35S启动子在转基因棉花中的表达 被引量:17
9
作者 焦改丽 孟钊红 +7 位作者 聂安全 南芝润 张换样 李俊峰 王娇娟 赵俊侠 李燕娥 郭三堆 《作物学报》 CAS CSCD 北大核心 2004年第11期1135-1139,共5页
利用GUS作为报告基因 ,通过GUS组织化学定位法检测棉花转化愈伤组织、体细胞胚 ,R0 代棉花根、茎、叶、花器官以及正在发育的胚GUS基因表达情况 ,详细阐述了CaMV 3 5S启动子在棉花细胞中的表达轮廓。结果表明 ,在愈伤组织细胞有丝分裂... 利用GUS作为报告基因 ,通过GUS组织化学定位法检测棉花转化愈伤组织、体细胞胚 ,R0 代棉花根、茎、叶、花器官以及正在发育的胚GUS基因表达情况 ,详细阐述了CaMV 3 5S启动子在棉花细胞中的表达轮廓。结果表明 ,在愈伤组织细胞有丝分裂和增殖过程中GUS基因能稳定表达并遗传给后代细胞 ;在根、茎、叶细胞中检测到GUS表达活性。在胚胎发育过程中 ,最早检测到GUS表达活性的为开花 1 1d的鱼雷胚和胚乳。随着胚胎的发育 ,GUS表达活性逐渐增强并扩展到子叶微管组织 ,表明CaMV 3 5S启动子是随着胚胎发育进程被逐渐调节的。在花粉和特殊的纤维细胞中 ,也检测到GUS的表达活性。资料证明 ,该启动子在棉花不同发育时期的大部分组织和细胞中都是表达的。 展开更多
关键词 gus基因 花椰菜花叶病毒(CaMV)35s启动子 转基因棉花 胚胎发育 表皮细胞
下载PDF
含35S启动子的粟酒裂殖酵母表达载体的构建及功能鉴定 被引量:3
10
作者 王贺 王丕武 +1 位作者 洪洋 宋冰 《生物技术通报》 CAS CSCD 北大核心 2011年第7期191-196,共6页
利用PCR技术从植物表达载体pBI121上先后克隆出GUS基因和35S-GUS-NOS基因序列,分别构建粟酒裂殖酵母重组表达载体pESP-2-GUS和pESP-2-35S.GUS.NOS,并将它们转化到粟酒裂殖酵母菌体中。通过对不同液体培养基中两种转化子的不同启动子启动... 利用PCR技术从植物表达载体pBI121上先后克隆出GUS基因和35S-GUS-NOS基因序列,分别构建粟酒裂殖酵母重组表达载体pESP-2-GUS和pESP-2-35S.GUS.NOS,并将它们转化到粟酒裂殖酵母菌体中。通过对不同液体培养基中两种转化子的不同启动子启动GUS基因表达产物的GUS染色分析与比较,证明连入的35S启动子能够替代pESP-2自身受维生素B1浓度调控的启动子,从而降低了培养基的成本,为今后工业上利用粟酒裂殖酵母发酵生产产品,提高经济效益,奠定了基础。 展开更多
关键词 粟酒裂殖酵母 35s启动子 载体改造 gus染色
下载PDF
SV40启动子在烟草体内的驱动特性研究 被引量:1
11
作者 宝力德 邸娜 +2 位作者 于秀敏 刘福军 王瑞刚 《甘肃农业大学学报》 CAS CSCD 北大核心 2015年第2期90-94,99,共6页
根据SV40启动子序列设计引物,PCR扩增该启动子后,成功构建了具有SV40启动子和GUS报告基因的植物表达载体pLpPSG,并将重组质粒转化到烟草叶片中.通过SV40启动子驱动的GUS基因在烟草叶片内的瞬时性表达,研究了该启动子在植物基因表达中的... 根据SV40启动子序列设计引物,PCR扩增该启动子后,成功构建了具有SV40启动子和GUS报告基因的植物表达载体pLpPSG,并将重组质粒转化到烟草叶片中.通过SV40启动子驱动的GUS基因在烟草叶片内的瞬时性表达,研究了该启动子在植物基因表达中的驱动特性.结果表明:SV40启动子可启动GUS基因在烟草体内的表达,其提高基因表达水平达到2×35S启动子的(88.5±19.2)%. 展开更多
关键词 烟草 sV40启动子 gus基因 瞬时表达 2×35s启动子
下载PDF
CaMV35S双启动子显著提高转基因在拟南芥中表达水平的研究(简报) 被引量:15
12
作者 郝林 曹军 《植物生理学通讯》 CSCD 2000年第6期517-519,共3页
以大肠杆菌 (E .coli)UidA为报道基因定量研究CaMV35S双启动子在拟南芥中的表达强度表明 ,35S双启动子的平均表达强度比 35S启动子高
关键词 CaMV35s双启动子 gus活性 拟南芥 转基因沉默
下载PDF
小鼠液胞H^+-ATPase 15 K启动子(M15 K)的克隆及其在植物体内的表达特性研究
13
作者 岳文冉 于秀敏 王瑞刚 《河南师范大学学报(自然科学版)》 CAS 北大核心 2014年第2期119-123,共5页
PCR克隆了小鼠液胞H+-ATPase 15K启动子,构建具有Kan抗性和GUS intron报告基因的植物表达载体LpPMG.通过M15K启动子指导的GUS intron基因在烟草叶片内的瞬时性表达,比较了其植物表达特性.结果表明:M15K启动子可启动GUS在植物体内的表达... PCR克隆了小鼠液胞H+-ATPase 15K启动子,构建具有Kan抗性和GUS intron报告基因的植物表达载体LpPMG.通过M15K启动子指导的GUS intron基因在烟草叶片内的瞬时性表达,比较了其植物表达特性.结果表明:M15K启动子可启动GUS在植物体内的表达.其表达活性相当于2×35S启动子的87.0%±17.3%. 展开更多
关键词 植物 M15 K启动子 gus 瞬时表达 增强型35s启动子
下载PDF
THE FUNCTIONAL RELATION BETWEEN ION CONCENTRATION IN WATER VAPOR CHEMISTRY AND THE BOND PARAMETER IN QUANTUM CHEMISTRY
14
作者 顾宏堪 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 1997年第1期88-91,共4页
The functional relation between"water vapor chemistry" ion concentration and the "quantum chemistry" bond parameter iswhere IZ /Z is specific electron affinity constant (as hydration force), IZ is ... The functional relation between"water vapor chemistry" ion concentration and the "quantum chemistry" bond parameter iswhere IZ /Z is specific electron affinity constant (as hydration force), IZ is last ionization potential, Z is ion valence, V is ion volume, N is coordination number(as hydration number), C is ion concentration (μg/L) in condensed wahter of water vapor, R is correlation coefficient, S is standard deviation, a and b are constants. The corresponding correlation curve shows that C has positive correlation to Iz/Z and negative correlation to VN. 展开更多
关键词 Water vapor CHEMIsTRY gu’s function
下载PDF
显示模块GU140×32F-7050的接口设计与应用
15
作者 孔文艳 李坚利 朱卯成 《兵工自动化》 2013年第4期91-93,共3页
为实现某控制装置文字和图形的显示,设计显示模块GU140×32F-7050的接口。硬件接口采用MC9S12DP512作为MPU,通过并行接口与显示模块连接;软件接口包括数据写入、初始化、显示、窗口定义和窗口选择。根据点阵图获取汉字、数字、字母... 为实现某控制装置文字和图形的显示,设计显示模块GU140×32F-7050的接口。硬件接口采用MC9S12DP512作为MPU,通过并行接口与显示模块连接;软件接口包括数据写入、初始化、显示、窗口定义和窗口选择。根据点阵图获取汉字、数字、字母或图形的图像数据,以2种方式调用显示接口函数得到相同的显示效果,并给出相应的程序代码。目前该显示模块已批量应用于某控制装置,实际应用表明,显示模块GU140×32F-7050工作稳定、可靠性高。 展开更多
关键词 显示模块 接口 gu140×32F-7050 MC9s12DP512
下载PDF
A Rare Case of Atypical Recalcitrant Hailey-Hailey Disease and a Literature Review
16
作者 Udoka Ogbuneke Emmanuel Odega +1 位作者 Yakub Wahab Ibrahim Mustapha Abubakar 《Case Reports in Clinical Medicine》 2022年第9期358-363,共6页
Hailey-Hailey disease also known as familial benign chronic pemphigus is a rare bullous genodermatosis that affects intertriginous area symmetrically. It presents with flaccid blisters, erosions and maceration resulti... Hailey-Hailey disease also known as familial benign chronic pemphigus is a rare bullous genodermatosis that affects intertriginous area symmetrically. It presents with flaccid blisters, erosions and maceration resulting in increased morbidity, reduced quality of life for affected patients. It is rare in occurrence with an incidence of rate of 1 in 50,000. It is diagnosed with a combination of clinical and histopathological findings. While there is no known cure, its relapsing remitting course can be managed with medication. This case describes an unusual presentation of familial benign chronic pemphigus with a late age of onset of symptoms, atypical distribution and resistant to multiple therapies. 展开更多
关键词 Internal Medicine dermatology Atypical Hailey-Hailey’s Disease Benign Chronic Pemphigus
下载PDF
经典型Kaposi肉瘤患者家庭中Kaposi肉瘤相关疱疹病毒的感染情况
17
作者 Guttman- Yassky E. Kra- Oz Z. +2 位作者 Dubnov J. R. Sarid 王琼 《世界核心医学期刊文摘(皮肤病学分册)》 2006年第2期25-25,共1页
关键词 Background: CLAssIC Kaposi’ s sARCOMA (CKs) primar
下载PDF
国家形象视域下基于语篇衔接的旅游文本翻译研究——以福州鼓山景区导游解说词英译为例
18
作者 王丽 《福建技术师范学院学报》 2023年第6期772-776,806,共6页
以鼓山导游解说词的英文翻译为例,在对比汉英语篇衔接模式差异的基础上,通过分析译者对导游词汉语文本中最为典型的用词重复现象和隐性句法连接的处理方式,探讨旅游文本翻译中词汇衔接与语法衔接手段对语篇衔接的促进作用,并提出旅游文... 以鼓山导游解说词的英文翻译为例,在对比汉英语篇衔接模式差异的基础上,通过分析译者对导游词汉语文本中最为典型的用词重复现象和隐性句法连接的处理方式,探讨旅游文本翻译中词汇衔接与语法衔接手段对语篇衔接的促进作用,并提出旅游文本翻译中的语篇衔接有助于构建积极、正面的中国国家形象。 展开更多
关键词 旅游文本翻译 语篇衔接 传播效果 国家形象 鼓山导游解说词
下载PDF
History of the Chinese Society of Dermatology: an overview
19
作者 ZHANG Jian-zhong ZHANG Xue-jun 《Chinese Medical Journal》 SCIE CAS CSCD 2009年第19期2258-2260,共3页
The history of Dermatology in China can be traced back to the middle of the 19th century when missionary doctors from America and Europe introduced Western medicine to China and established several hospitals where der... The history of Dermatology in China can be traced back to the middle of the 19th century when missionary doctors from America and Europe introduced Western medicine to China and established several hospitals where dermatology either as a division or as a group in the department of medicine. 展开更多
关键词 Chinese society of dermatology Langerhan's cells GENODERMATOsIs
原文传递
詹红生诊治颈椎病经验 被引量:17
20
作者 胡零三 詹红生 +3 位作者 熊轶喆 陈博 赵伽琛 廉由之 《上海中医药杂志》 2018年第7期26-28,共3页
探讨詹红生教授论治颈椎病的临床经验。认为其主要病机为筋出槽骨错缝,并构建临床诊疗评估指标体系,治疗以中药针刺内调气血不通、手法导引外治筋骨失和,以恢复颈椎"骨正筋柔、气血以流"生理状态。
关键词 颈椎病 筋出槽骨错缝 医家经验 詹红生
下载PDF
上一页 1 2 4 下一页 到第
使用帮助 返回顶部