[Objectives] To optimize the extraction process and determine the total triterpenoids from Semen Trichosanthis.[Methods]The Box-behnken response surface methodology was applied to optimize the extraction conditions of...[Objectives] To optimize the extraction process and determine the total triterpenoids from Semen Trichosanthis.[Methods]The Box-behnken response surface methodology was applied to optimize the extraction conditions of total triterpenoids in Semen Trichosanthis.And the spectrophotometry was used to determine the content of total triterpenoids in Semen Trichosanthis.[Results] The optimal extraction conditions were solid to liquid ratio of 40∶ 1,ultrasonic time of 20 min,and ultrasonic power of 100 W.Under these conditions,the content of total triterpenoids in Semen Trichosanthis was significantly different among the 10 production areas,among which the No.9 production area(Anhui 1) had the highest total triterpenoids content of 45.71 mg/g,while No.7 production area(Shandong) had the lowest total triterpenoids content of 15.22 mg/g.[Conclusions]The Box-behnken response surface methodology is reliable for the extraction of total triterpenoids in Semen Trichosanthis,and the spectrophotometry is proper for determining the content of total triterpenoids in Semen Trichosanthis.There are large differences in the content of total triterpenoids in Semen Trichosanthis produced in different areas.展开更多
Objective:To optimize the extraction process of total flavones in Trichosanthis Fructus(composed of Trichosanthis pericarpium and Trichosanthis semen in certain proportion).Methods:The effects of the mixture ratio of ...Objective:To optimize the extraction process of total flavones in Trichosanthis Fructus(composed of Trichosanthis pericarpium and Trichosanthis semen in certain proportion).Methods:The effects of the mixture ratio of Trichosanthis pericarpium and Trichosanthis semen,ethanol concentration,ultrasonic extraction time and extraction temperature on the extraction rate of total flavonoids in Trichosanthis Fructus were investigated.The extraction process of total flavonoids in Trichosanthis Fructus was optimized by Box-Behnken response surface method combined with differential spectrophotometry.Results:The optimum extraction conditions of total flavonoids in Trichosanthis Fructus were as follows:The mixture ratio of Trichosanthis pericarpium and Trichosanthis semen was 4:6,the ethanol concentration was 70%,the ultrasonic extraction time was 60min and the extraction temperature was 40℃.Conclusion:Box-Behnken response surface method combined with differential spectrophotometry can optimize the extraction process of total flavonoids from Trichosanthis Fructus,which can provide reference for the extraction and application of total flavonoids in Trichosanthis Fructus.展开更多
分析瓜蒌子变质过程中油脂类成分的变化,筛选差异标志物,为瓜蒌子的质量控制和临床合理使用提供科学依据。采用加速实验加快瓜蒌子变质进程,利用气相色谱质谱联用(GC-MS)技术鉴定瓜蒌子中油脂类化学成分,通过NIST17谱库、化合物精确相...分析瓜蒌子变质过程中油脂类成分的变化,筛选差异标志物,为瓜蒌子的质量控制和临床合理使用提供科学依据。采用加速实验加快瓜蒌子变质进程,利用气相色谱质谱联用(GC-MS)技术鉴定瓜蒌子中油脂类化学成分,通过NIST17谱库、化合物精确相对分子质量及文献进行化合物解析,并结合聚类分析(Cluster analysis,CA)、主成分分析(Principal component analysis,PCA)和正交偏最小二乘判别分析(Orthogonal partial least squares discriminant analysis,OPLS-DA)对色谱峰进行综合处理和分析。研究结果显示:基于GC-MS技术共鉴定出新鲜瓜蒌子中22个化合物,随着加速实验天数增加,瓜蒌子在变质过程中新鉴定出5个化合物。利用OPLS-DA模型中VIP分析,发现瓜蒌子变质过程中6个色谱峰的VIP(Variable importance in the projection)均大于1.0,分别为α-桐酸、α-亚麻酸、油酸、亚油酸、棕榈酸和瓜蒌酸,其可作为瓜蒌子变质过程中差异标志物。通过GC-MS技术总结瓜蒌子脂肪酸在变质过程中的变化规律,利用化学模式识别筛选出6个差异标志物,研究结果可为瓜蒌子饮片及其中药制剂的质量控制奠定基础。展开更多
基金Supported by Project of Department of Education of Liaoning Province(L2017lkyfwdf-05)Natural Science Foundation Project of Department of Education of Liaoning Province(20180551223&2016003003)
文摘[Objectives] To optimize the extraction process and determine the total triterpenoids from Semen Trichosanthis.[Methods]The Box-behnken response surface methodology was applied to optimize the extraction conditions of total triterpenoids in Semen Trichosanthis.And the spectrophotometry was used to determine the content of total triterpenoids in Semen Trichosanthis.[Results] The optimal extraction conditions were solid to liquid ratio of 40∶ 1,ultrasonic time of 20 min,and ultrasonic power of 100 W.Under these conditions,the content of total triterpenoids in Semen Trichosanthis was significantly different among the 10 production areas,among which the No.9 production area(Anhui 1) had the highest total triterpenoids content of 45.71 mg/g,while No.7 production area(Shandong) had the lowest total triterpenoids content of 15.22 mg/g.[Conclusions]The Box-behnken response surface methodology is reliable for the extraction of total triterpenoids in Semen Trichosanthis,and the spectrophotometry is proper for determining the content of total triterpenoids in Semen Trichosanthis.There are large differences in the content of total triterpenoids in Semen Trichosanthis produced in different areas.
基金Anhui Universities Provincial Key Project of Natural Science Research(No.KJ2016SD60,KJ2015ZD41)。
文摘Objective:To optimize the extraction process of total flavones in Trichosanthis Fructus(composed of Trichosanthis pericarpium and Trichosanthis semen in certain proportion).Methods:The effects of the mixture ratio of Trichosanthis pericarpium and Trichosanthis semen,ethanol concentration,ultrasonic extraction time and extraction temperature on the extraction rate of total flavonoids in Trichosanthis Fructus were investigated.The extraction process of total flavonoids in Trichosanthis Fructus was optimized by Box-Behnken response surface method combined with differential spectrophotometry.Results:The optimum extraction conditions of total flavonoids in Trichosanthis Fructus were as follows:The mixture ratio of Trichosanthis pericarpium and Trichosanthis semen was 4:6,the ethanol concentration was 70%,the ultrasonic extraction time was 60min and the extraction temperature was 40℃.Conclusion:Box-Behnken response surface method combined with differential spectrophotometry can optimize the extraction process of total flavonoids from Trichosanthis Fructus,which can provide reference for the extraction and application of total flavonoids in Trichosanthis Fructus.
文摘分析瓜蒌子变质过程中油脂类成分的变化,筛选差异标志物,为瓜蒌子的质量控制和临床合理使用提供科学依据。采用加速实验加快瓜蒌子变质进程,利用气相色谱质谱联用(GC-MS)技术鉴定瓜蒌子中油脂类化学成分,通过NIST17谱库、化合物精确相对分子质量及文献进行化合物解析,并结合聚类分析(Cluster analysis,CA)、主成分分析(Principal component analysis,PCA)和正交偏最小二乘判别分析(Orthogonal partial least squares discriminant analysis,OPLS-DA)对色谱峰进行综合处理和分析。研究结果显示:基于GC-MS技术共鉴定出新鲜瓜蒌子中22个化合物,随着加速实验天数增加,瓜蒌子在变质过程中新鉴定出5个化合物。利用OPLS-DA模型中VIP分析,发现瓜蒌子变质过程中6个色谱峰的VIP(Variable importance in the projection)均大于1.0,分别为α-桐酸、α-亚麻酸、油酸、亚油酸、棕榈酸和瓜蒌酸,其可作为瓜蒌子变质过程中差异标志物。通过GC-MS技术总结瓜蒌子脂肪酸在变质过程中的变化规律,利用化学模式识别筛选出6个差异标志物,研究结果可为瓜蒌子饮片及其中药制剂的质量控制奠定基础。
