Objective The main pathological feature of immunoglobulin A nephropathy(IgAN),an autoimmune kidney disease,is the deposition of IgA immune complexes,accompanied by mesangial cell proliferation and elevated urine prote...Objective The main pathological feature of immunoglobulin A nephropathy(IgAN),an autoimmune kidney disease,is the deposition of IgA immune complexes,accompanied by mesangial cell proliferation and elevated urine protein.The Guben Tongluo formula(GTF)is a traditional Chinese medicine prescription,which has predominant protective effects on IgAN.However,the therapeutic mechanism of the GTF in IgAN remains elusive.The present study aimed to determine the effects of GTF in treating IgAN via regulating the TLR4/MyD88/NF-κB pathway.Methods In the present study,lamina propria B lymphocytes were treated with different concentrations of lipopolysaccharide(LPS)(0,1,5,10 and 20 ng/mL).Flow cytometry was used to define positive CD86+CD19+cells.CCK-8 assay was used to examine cell proliferation.RNAi was used to induce TLR4 silencing.qRT-PCR and Western blotting were used to determine gene expression.Results It was found that the LPS dose-dependently increased the content of IgA and galactose-deficient IgA1(Gd-IgA),the levels of TLR4,Cosmc,MyD88 and phosphorylated(p)-NF-κB,and the ratio of CD86+CD19+and IgA-producing B cells.However,the TLR4 knockdown reversed the role of LPS.This suggests that TLR4 mediates the effects of LPS on lamina propria B lymphocytes.Furthermore,the GTF could dose-dependently counteract the effects of LPS and TLR4 overexpression on lamina propria B lymphocytes through the TLR4/MyD88/NF-κB pathway.Conclusion Collectively,these results demonstrate that the GTF can regulate the TLR4/MyD88/NF-κB pathway to treat IgAN model lamina propria B lymphocytes stimulated by LPS.展开更多
目的:探究固本通络汤联合激素及环磷酰胺治疗难治性肾病综合征(Refractory Nephotic Syndrome,RNS)的临床效果。方法:选取2017年9月-2019年12月于天门市中医医院接受治疗的156例RNS患者,按随机数字表法分为两组,各78例。对照组行激素及...目的:探究固本通络汤联合激素及环磷酰胺治疗难治性肾病综合征(Refractory Nephotic Syndrome,RNS)的临床效果。方法:选取2017年9月-2019年12月于天门市中医医院接受治疗的156例RNS患者,按随机数字表法分为两组,各78例。对照组行激素及环磷酰胺治疗,观察组在对照组基础上联合固本通络方治疗。对比两组临床疗效、治疗前后中医症状积分、肾功能各指标水平[血尿素氮(Blood Ure Nitrogen,BUN)、血清肌酐(Serum Creatinine,Scr)、血清白蛋白(Albumin,ALB)及24h尿蛋白定量(24-hour Urinary Protein Quantity,24 h UP)]、不良反应。结果:相比于对照组,观察组总有效率高、治疗后中医证候积分较低,差异有统计学意义(P<0.05);观察组治疗后BUN、Scr、24 h UP均低于对照组,ALB含量高于对照组,差异有统计学意义(P<0.05);观察组不良反应少于对照组,差异有统计学意义(P<0.05)。结论:固本通络汤联合激素及环磷酰胺在RNS治疗中疗效确切,可有效降低中医症状积分,改善肾功能各指标水平,利于促进预后恢复,值得推广应用。展开更多
基金supported by the National Natural Science Foundation of China(No.81904124)the Scientific Project of Shanghai Sanitation and Health Committee(No.20204Y0191)the projects of Shanghai Science and Technology Commission(No.22Y31920200).
文摘Objective The main pathological feature of immunoglobulin A nephropathy(IgAN),an autoimmune kidney disease,is the deposition of IgA immune complexes,accompanied by mesangial cell proliferation and elevated urine protein.The Guben Tongluo formula(GTF)is a traditional Chinese medicine prescription,which has predominant protective effects on IgAN.However,the therapeutic mechanism of the GTF in IgAN remains elusive.The present study aimed to determine the effects of GTF in treating IgAN via regulating the TLR4/MyD88/NF-κB pathway.Methods In the present study,lamina propria B lymphocytes were treated with different concentrations of lipopolysaccharide(LPS)(0,1,5,10 and 20 ng/mL).Flow cytometry was used to define positive CD86+CD19+cells.CCK-8 assay was used to examine cell proliferation.RNAi was used to induce TLR4 silencing.qRT-PCR and Western blotting were used to determine gene expression.Results It was found that the LPS dose-dependently increased the content of IgA and galactose-deficient IgA1(Gd-IgA),the levels of TLR4,Cosmc,MyD88 and phosphorylated(p)-NF-κB,and the ratio of CD86+CD19+and IgA-producing B cells.However,the TLR4 knockdown reversed the role of LPS.This suggests that TLR4 mediates the effects of LPS on lamina propria B lymphocytes.Furthermore,the GTF could dose-dependently counteract the effects of LPS and TLR4 overexpression on lamina propria B lymphocytes through the TLR4/MyD88/NF-κB pathway.Conclusion Collectively,these results demonstrate that the GTF can regulate the TLR4/MyD88/NF-κB pathway to treat IgAN model lamina propria B lymphocytes stimulated by LPS.
文摘目的:探究固本通络汤联合激素及环磷酰胺治疗难治性肾病综合征(Refractory Nephotic Syndrome,RNS)的临床效果。方法:选取2017年9月-2019年12月于天门市中医医院接受治疗的156例RNS患者,按随机数字表法分为两组,各78例。对照组行激素及环磷酰胺治疗,观察组在对照组基础上联合固本通络方治疗。对比两组临床疗效、治疗前后中医症状积分、肾功能各指标水平[血尿素氮(Blood Ure Nitrogen,BUN)、血清肌酐(Serum Creatinine,Scr)、血清白蛋白(Albumin,ALB)及24h尿蛋白定量(24-hour Urinary Protein Quantity,24 h UP)]、不良反应。结果:相比于对照组,观察组总有效率高、治疗后中医证候积分较低,差异有统计学意义(P<0.05);观察组治疗后BUN、Scr、24 h UP均低于对照组,ALB含量高于对照组,差异有统计学意义(P<0.05);观察组不良反应少于对照组,差异有统计学意义(P<0.05)。结论:固本通络汤联合激素及环磷酰胺在RNS治疗中疗效确切,可有效降低中医症状积分,改善肾功能各指标水平,利于促进预后恢复,值得推广应用。
