目的:通过体内实验研究己烯雌酚(DES)对小鼠睾丸引带中胰岛素样因子3受体LGR8的影响,从而探讨外源性雌激素对小鼠睾丸下降的影响。方法:8～10周龄KM孕鼠随机分为正常组、空白对照组和实验组(0.1、1.0、10、100μg/kg DES 4个剂量组),共6...目的:通过体内实验研究己烯雌酚(DES)对小鼠睾丸引带中胰岛素样因子3受体LGR8的影响,从而探讨外源性雌激素对小鼠睾丸下降的影响。方法:8～10周龄KM孕鼠随机分为正常组、空白对照组和实验组(0.1、1.0、10、100μg/kg DES 4个剂量组),共6组,每组20只。于孕9～17 d每天分别给予实验组妊娠小鼠不同剂量的DES(0.1、1、10、100μg/kg),空白对照组给予等体积DMSO+生理盐水,正常组不给药。应用免疫组化和RT-PCR分别检测胎鼠和幼鼠睾丸引带中LGR8蛋白和mRNA的表达。结果:HE染色可见胎鼠正常组、对照组睾丸引带发育良好,中间的间叶组织和外周的肌源细胞之间分界清楚;实验组可见睾丸引带发育不良,间叶组织和肌源细胞之间无明显分界,组织结构紊乱。幼鼠正常组和实验组睾丸引带发育未见明显不同。LGR8表达于睾丸引带肌源细胞和间叶组织细胞,以肌源细胞表达为主。实验组LGR8阳性表达较正常组弱,胎鼠各实验组和幼鼠DES 1、10、100μg组与同发育阶段的正常组相比均有统计学意义(P<0.05)。DES高剂量(10、100μg)组与同发育阶段的正常组相比LGR8 mRNA表达增加(P<0.05)。各实验组PCR产物测序均未见明显突变。结论:DES可影响小鼠睾丸引带LGR8 mRNA和蛋白的表达,DES可能通过影响INSL3-LGR8信号系统干扰睾丸引带的发育,进而影响睾丸正常下降。展开更多
Aim: To study the effect of prenatal exposure to diethylstilbestrol (DES) and the role of actin and proliferating cell nuclear antigen (PCNA) on testicular gubernaculum development in fetal male Kunming mice. Methods:...Aim: To study the effect of prenatal exposure to diethylstilbestrol (DES) and the role of actin and proliferating cell nuclear antigen (PCNA) on testicular gubernaculum development in fetal male Kunming mice. Methods: Pregnant mice were randomly assigned to 6 groups and injected with DES subcutaneously from gestational day 9 (E9) to day 17 (E17) at doses of 0, 25, 50, 100, 200 μg·kg-1·d-1 in 0.2 mL dimethyl sulfoxide (DMSO). On E17 they were sacrificed and fetuses quickly removed for fixation. Male fetuses were sliced on serial coronal plane. Histologi-cal changes were observed under the light microscope (LM) and ultrastructural changes with the scanning and transmission electron microscopes (SEM & TEM). The expression intensity of actin and PCNA in the gubernacula was quantitated by immunohistochemistry. Results: The mortality of the fetuses was higher in the DES-treated groups than that in the DMSO and saline controls (P<0.05). Under LM the gubernacula were seen to be poorly developed with smaller bulbs. On SEM the bulbs lose the clear demarcation between the mesenchymal inner core and the muscular outer layer and looked like a small cone instead of the normal cylindrical appearance. On TEM there were some smaller disordered myofibrils and sparse cytoplasmic organelles in the gubernacular muscular cells of the treated groups. The expression intensity of actin and PCNA in the gubernacula was significantly weaker in the treated groups than that in the DMSO and saline controls (P<0.05). Conclusion: DES induces underdevelopment of the gubernacula in a dose-dependent manner in fetal male mice and down regulates the actin and PCNA expression.展开更多
Introduction: Total unilateral ectopy of the scrotum can be defined by the abnormal congenital anatomical position of the scrotum and its contents. The aim of the study is to describe the diagnostic and therapeutic ap...Introduction: Total unilateral ectopy of the scrotum can be defined by the abnormal congenital anatomical position of the scrotum and its contents. The aim of the study is to describe the diagnostic and therapeutic approach of a total unilateral ectopy of the right scrotum. Methods: This is a clinical observation of a 46-year-old patient with a right abdominal mass evolving from birth. Analyzing the cluster of arguments clinically, operatively and anatomopathologically, allowed us to retain the diagnosis of a total unilateral ectopy of the right scrotum. The informed consent of the patient was obtained before the use of the images for scientific purposes. Conclusion: the unilateral total ectopy of the right scrotum is a rare congenital malformation of the external genitalia, which clinical diagnosis is difficult. Its management is surgical.展开更多
目的研究戊酸雌二醇对大鼠胎鼠睾丸与睾丸引带形态结构及功能的影响,探讨睾丸下降不全的原因。方法30只健康SD受孕大鼠随机均分为4组:对照组及低、中、高剂量组。戊酸雌二醇以剂量0.8~2.0 mg/(kg·d)灌胃作用于怀孕6~15 d SD母鼠,...目的研究戊酸雌二醇对大鼠胎鼠睾丸与睾丸引带形态结构及功能的影响,探讨睾丸下降不全的原因。方法30只健康SD受孕大鼠随机均分为4组:对照组及低、中、高剂量组。戊酸雌二醇以剂量0.8~2.0 mg/(kg·d)灌胃作用于怀孕6~15 d SD母鼠,分娩后正常喂养雄性子代,出生60 d测量附睾、睾丸的脏器系数(附睾、睾丸重量/大鼠体重100),进行睾丸组织学切片观察生精小管的形态学变化,测量生精小管直径。结果中、高剂量组睾丸均有一定程度的下降不全,光镜下睾丸生精小管、支持细胞存在明显的发育障碍,睾丸Leydig细胞明显增生;附睾、睾丸脏器系数、睾丸生精小管直径较对照组及低剂量给药组差异有统计学意义(P<0.05)。结论雌激素可导致睾丸引带功能障碍,使睾丸下降发生异常而诱导隐睾发生;同时造成睾丸Sertoli细胞、睾丸Leydig细胞和生精细胞发育障碍及功能改变。展开更多
文摘Aim: To study the effect of prenatal exposure to diethylstilbestrol (DES) and the role of actin and proliferating cell nuclear antigen (PCNA) on testicular gubernaculum development in fetal male Kunming mice. Methods: Pregnant mice were randomly assigned to 6 groups and injected with DES subcutaneously from gestational day 9 (E9) to day 17 (E17) at doses of 0, 25, 50, 100, 200 μg·kg-1·d-1 in 0.2 mL dimethyl sulfoxide (DMSO). On E17 they were sacrificed and fetuses quickly removed for fixation. Male fetuses were sliced on serial coronal plane. Histologi-cal changes were observed under the light microscope (LM) and ultrastructural changes with the scanning and transmission electron microscopes (SEM & TEM). The expression intensity of actin and PCNA in the gubernacula was quantitated by immunohistochemistry. Results: The mortality of the fetuses was higher in the DES-treated groups than that in the DMSO and saline controls (P<0.05). Under LM the gubernacula were seen to be poorly developed with smaller bulbs. On SEM the bulbs lose the clear demarcation between the mesenchymal inner core and the muscular outer layer and looked like a small cone instead of the normal cylindrical appearance. On TEM there were some smaller disordered myofibrils and sparse cytoplasmic organelles in the gubernacular muscular cells of the treated groups. The expression intensity of actin and PCNA in the gubernacula was significantly weaker in the treated groups than that in the DMSO and saline controls (P<0.05). Conclusion: DES induces underdevelopment of the gubernacula in a dose-dependent manner in fetal male mice and down regulates the actin and PCNA expression.
文摘Introduction: Total unilateral ectopy of the scrotum can be defined by the abnormal congenital anatomical position of the scrotum and its contents. The aim of the study is to describe the diagnostic and therapeutic approach of a total unilateral ectopy of the right scrotum. Methods: This is a clinical observation of a 46-year-old patient with a right abdominal mass evolving from birth. Analyzing the cluster of arguments clinically, operatively and anatomopathologically, allowed us to retain the diagnosis of a total unilateral ectopy of the right scrotum. The informed consent of the patient was obtained before the use of the images for scientific purposes. Conclusion: the unilateral total ectopy of the right scrotum is a rare congenital malformation of the external genitalia, which clinical diagnosis is difficult. Its management is surgical.
文摘目的研究戊酸雌二醇对大鼠胎鼠睾丸与睾丸引带形态结构及功能的影响,探讨睾丸下降不全的原因。方法30只健康SD受孕大鼠随机均分为4组:对照组及低、中、高剂量组。戊酸雌二醇以剂量0.8~2.0 mg/(kg·d)灌胃作用于怀孕6~15 d SD母鼠,分娩后正常喂养雄性子代,出生60 d测量附睾、睾丸的脏器系数(附睾、睾丸重量/大鼠体重100),进行睾丸组织学切片观察生精小管的形态学变化,测量生精小管直径。结果中、高剂量组睾丸均有一定程度的下降不全,光镜下睾丸生精小管、支持细胞存在明显的发育障碍,睾丸Leydig细胞明显增生;附睾、睾丸脏器系数、睾丸生精小管直径较对照组及低剂量给药组差异有统计学意义(P<0.05)。结论雌激素可导致睾丸引带功能障碍,使睾丸下降发生异常而诱导隐睾发生;同时造成睾丸Sertoli细胞、睾丸Leydig细胞和生精细胞发育障碍及功能改变。
