Effects of Guhanyangshengjing Tablet on Testosterone Synthesis and Expression of SYCP3 in the Testis of Aging Male Rats

Objective: The objective of this article is to investigate the effect of Guhanyangshengjing Tablet (GT) on expression of synaptonemal complex protein 3 (SYCP3), a meiotic marker, in the testis tissue of aging male rats. Methods: Forty aging male rats were randomly assigned into 4 equal groups (n = 10 per group). Rats in each group were treated with GT at dose of 0 (control), 1.5 g/kg, 3.0 g/kg or 4.5 g/kg respectively by gavage daily for 30 days. At the end of the experiment, all animals were sacrificed and the blood samples were drawn to evaluate serum testosterone levels. The reproductive organs of each rat were taken and weighted. The right testis of each rat was removed for the analysis of intratesticular testosterone (ITT) concentrations, and the left one was used for immunohistochemical staining. Results: Compared with the control, reproductive organs’ weights, serum testosterone levels, ITT concentrations, quality of sperm, and expression of SYCP3 in the GT-treated groups were all improved in a dose-dependent manner. Conclusion: GT can improve testosterone synthesis and promote spermatogenesis simultaneously, indicating that GT is suitable for late-onset hypogonadism (LOH) patients with fertility requirements.

变异性哮喘患者血清可溶性细胞间粘附分子和内皮素水平及其临床意义

用放免法对３５ 例变异性哮喘患者外周血进行了可溶性细胞间粘附分子（ｓＩＣＡＭ１）和内皮素（ＥＴ１）的检测。与健康对照组比较，ｓＩＣＡＭ１ 和ＥＴ１ 水平显著增高（Ｐ＜ ００１）；与普通型哮喘比较，发作期差异不显著（Ｐ＞ ００５），缓解期差异非常显著（Ｐ＜ ００１）。提示二者在哮喘病发作中起重要作用。

HPLC法测定古汉养生精片(口服液)中淫羊藿苷的含量

目的：建立高效液相色谱法测定古汉养生精片和古汉养生精口服液中淫羊藿苷含量方法。方法：采用Hypersil BDS C18柱（250mm×4.6mm,5μm）,以甲醇-1.5%冰醋酸（58：42）为流动相,流速：1.0ml/min,检测波长270nm。结果：淫羊藿苷在0.088-1.760μg范围内与峰面积线性关系良好（r=0.9991）,平均回收率为98.0%,RSD为1.09%。结论：所建高效液相色谱法简单,准确,专属性强,重现性好,可用于古汉养生精片（口服液）的质量控制。

UPLC-MS法同时测定古汉养生精口服液中5种化学成分

目的建立超高效液相色谱-串联质谱法同时测定古汉养生精口服液中淫羊藿苷、黄芪甲苷、人参皂苷Rb1、Re和Rg1的含量。方法采用Waters Acquity UPLC C18色谱柱(2.1mm×50mm,1.7μm);以乙腈-0.1%甲酸水溶液(15∶85)为流动相进行洗脱,流速0.2mL·min^(-1),以电喷雾离子源多反应监测(MRM)方式负离子模式检测离子对m/z 721.3→513.40(淫羊藿苷),正离子模式分别检测离子对m/z 807.3→627.30(黄芪甲苷)、m/z 1131.4→365.46(人参皂苷Rb1)、m/z 969.5→789.38(人参皂苷Re)、m/z 823.5→643.54(人参皂苷Rg1)。结果淫羊藿苷、黄芪甲苷、人参皂苷Rb1、Re和Rg1分别在1860.0~37200.0ng·mL^(-1)、172.6~3451.8ng·mL^(-1)、95.8~1915.2ng·mL^(-1)、89.4~1787.0ng·mL^(-1)、98.4~1968.0ng·mL^(-1)浓度范围内线性关系良好,回收率均在99.31%~100.75%之间,RSD均小于1.55%(n=6)结论此法简单、快速、准确可靠,可用于古汉养生精口服液的质量控制。

古汉养生精对20月龄雌性大鼠血清一氧化氮、性激素、超氧化物歧化酶的影响

目的 探讨衰老雌性大鼠血清中一氧化氮 (NO)、性激素 (Sexhormone)和超氧化物歧化酶(SOD)含量的变化与衰老的相关关系。方法 采用硝酸还原酶法和放免分析等方法 ,检测血清中NO、Sexhormone、SOD等含量的变化。结果 古汉养生精能有效升高 2 0月龄雌性大鼠血清NO、Sexhormone、SOD含量 ,降低脂质过氧化物 (LPO)水平。

益欣康泰胶囊对人体睾酮、雌二醇的影响

目的：观察益欣康泰胶囊对人体睾酮和雌二醇水平的影响。方法：采用放射免疫法观察了海拔３ ３００ｍ 地区６７ 例健康男性服用益欣康泰胶囊和古汉养生精前后血浆睾酮（Ｔ）和雌二醇（Ｅ２）的变化。结果：健康男性服用益欣康泰胶囊后血浆睾酮含量明显升高，与服药前比较Ｐ＜ ００１；服用古汉养生精组健康男性Ｔ、Ｅ２ 有升高趋势，与服药前比较无显著性差异。两服药组相比较，益欣康泰胶囊升高体内Ｔ含量和Ｔ／Ｅ比值的作用优于古汉养生精组Ｐ＜ ００１。