Objective To explore the transitive regularity of holistic constituents from the crude slices of the medicinal raw materials(MCS)to the formula granules(FG),fufang decoction(FD),and finally,the concentrated pills(CP)o...Objective To explore the transitive regularity of holistic constituents from the crude slices of the medicinal raw materials(MCS)to the formula granules(FG),fufang decoction(FD),and finally,the concentrated pills(CP)of Liuwei Dihuang Fufang(六味地黄复方,LWDHF).Methods Samples for MCS,FG,FD,and CP of LWDHF were obtained,and a fingerprint data-base was established using high-performance liquid chromatography(HPLC),by separating the samples in an XB-C18 column and analyzing the transitive regularity of components us-ing the total quantum statistical moment(TQSM),including total quantum zero moment(AUCT),total quantum first moment(MRTT),total quantum second moment(VRTT),and its similarity approach.The AUCT,MRTT,and VRTT were calculated based on the representative HPLC chromatograms of FG,FD,and CP of LWDHF.Results AUCT of FG,FD,and CP of LWDHF was 71804,46553,and 144646μV·s,respectively;MRTT was 14.43,14.54,and 18.85 min,respectively;and VRTT was 106.98,112.84,and 269.12 min2,respectively.Comparing the similarity of FG/FD,FG/CP and FD/CP of LWDHF,the TQSM similarity values were 98.66%,76.62%,and 75.37%,respectively,whereas the tradi-tional similarity evaluation values were 98.68%,85.43%,and 85.60%,respectively.Conclusion The results perform little distinction in the total composition between FG and FD,whereas some distinction existed between FD and CP.Experimental evidence,therefore indicates that FG could be used as the alternative of MCS in clinical applications.展开更多
目的:本研究以没食子酸、5-羟甲基糠醛、莫诺苷、马钱苷、芍药苷5个成分的含量为溶出指标,建立多成分指标评价六味地黄浓缩丸溶出度的方法。方法:测定条件为浆法,以900 m L 0.1 mol·L-1盐酸作为溶出介质,转速100 r·min-1,温度...目的:本研究以没食子酸、5-羟甲基糠醛、莫诺苷、马钱苷、芍药苷5个成分的含量为溶出指标,建立多成分指标评价六味地黄浓缩丸溶出度的方法。方法:测定条件为浆法,以900 m L 0.1 mol·L-1盐酸作为溶出介质,转速100 r·min-1,温度(37.0±0.5)℃。采用RP-HPLC外标法定量,Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.02%三氟乙酸溶液为流动相,梯度洗脱;流速为1.0 m L·min-1;柱温为30℃;检测波长:238 nm。结果:该厂家10个批次的六味地黄浓缩丸中5个成分的溶出曲线相似因子?2大部分高于50,表明该厂家不同批次间六味地黄浓缩丸的溶出度差异性较小。结论:该方法简便,重复性良好,适用于六味地黄浓缩丸中5个成分的溶出度测定。展开更多
基金Hunan Provincial Natural Science Foundation of China(2019JJ40220 and 2021JJ30514)Hunan Provincial Administration of Traditional Chinese Medicine(2021204and 2021073)+1 种基金Scientific Research Fund of Hunan Provincial Education Department(2021204 and 2021073)Pharmaceutical Open Fund of Domestic First-class Disciplines(Cultivation)of Hunan Province(2018YX11)。
文摘Objective To explore the transitive regularity of holistic constituents from the crude slices of the medicinal raw materials(MCS)to the formula granules(FG),fufang decoction(FD),and finally,the concentrated pills(CP)of Liuwei Dihuang Fufang(六味地黄复方,LWDHF).Methods Samples for MCS,FG,FD,and CP of LWDHF were obtained,and a fingerprint data-base was established using high-performance liquid chromatography(HPLC),by separating the samples in an XB-C18 column and analyzing the transitive regularity of components us-ing the total quantum statistical moment(TQSM),including total quantum zero moment(AUCT),total quantum first moment(MRTT),total quantum second moment(VRTT),and its similarity approach.The AUCT,MRTT,and VRTT were calculated based on the representative HPLC chromatograms of FG,FD,and CP of LWDHF.Results AUCT of FG,FD,and CP of LWDHF was 71804,46553,and 144646μV·s,respectively;MRTT was 14.43,14.54,and 18.85 min,respectively;and VRTT was 106.98,112.84,and 269.12 min2,respectively.Comparing the similarity of FG/FD,FG/CP and FD/CP of LWDHF,the TQSM similarity values were 98.66%,76.62%,and 75.37%,respectively,whereas the tradi-tional similarity evaluation values were 98.68%,85.43%,and 85.60%,respectively.Conclusion The results perform little distinction in the total composition between FG and FD,whereas some distinction existed between FD and CP.Experimental evidence,therefore indicates that FG could be used as the alternative of MCS in clinical applications.
文摘目的:本研究以没食子酸、5-羟甲基糠醛、莫诺苷、马钱苷、芍药苷5个成分的含量为溶出指标,建立多成分指标评价六味地黄浓缩丸溶出度的方法。方法:测定条件为浆法,以900 m L 0.1 mol·L-1盐酸作为溶出介质,转速100 r·min-1,温度(37.0±0.5)℃。采用RP-HPLC外标法定量,Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.02%三氟乙酸溶液为流动相,梯度洗脱;流速为1.0 m L·min-1;柱温为30℃;检测波长:238 nm。结果:该厂家10个批次的六味地黄浓缩丸中5个成分的溶出曲线相似因子?2大部分高于50,表明该厂家不同批次间六味地黄浓缩丸的溶出度差异性较小。结论:该方法简便,重复性良好,适用于六味地黄浓缩丸中5个成分的溶出度测定。
