Objective:This article uses the method of network pharmacology to study the related mechanism of Guilu Erxian Gum in the treatment of osteoporosis.Methods:Based on the TCMSP database,ETCM database,chemistry database,a...Objective:This article uses the method of network pharmacology to study the related mechanism of Guilu Erxian Gum in the treatment of osteoporosis.Methods:Based on the TCMSP database,ETCM database,chemistry database,and DrugBank database,the potential active ingredients and related targets of Guilu Erxian Gum were obtained.The known therapeutic targets of osteoporosis were obtained from OMIM and Genecards databases,and the STRING database was used.A protein interaction network(PPI)of active ingredients-disease targets was established,and the topological parameters of the network were analyzed using Cytoscape 3.8.0 software to obtain key active ingredients and their targets.In R4.0.2,the Bioconductor data package was used to analyze the GO biological function and KEGG pathway analysis of key targets,and obtain the effective ingredients and targets of Guilu Erxian Gum for treating osteoporosis.Results:The prediction results show that Guilu Erxian Gum has 87 active ingredients and 2305 targets,and there are 4141 known therapeutic targets for osteoporosis.The two act together to obtain a total of 71 PPI core genes.The GO biological process analysis yielded 95 entries,and the KEGG pathway analysis yielded 115 pathways.Conclusion:The analysis results show that Guilu Erxian Gum may play an anti-osteoporosis effect by regulating inflammatory factors,promoting osteoblast differentiation,inhibiting osteoclast formation,and improving microcirculation.The pathways involved include TNF signaling pathways,IL-17 signaling pathway,NF-κB signaling pathway,HIF-1 signaling pathway,MAPK signaling pathway,PI3K-Akt signaling pathway,etc.This study provides a theoretical basis for further elucidating the pharmacological mechanism of Guilu Erxian Gum in the treatment of osteoporosis.展开更多
目的观察龟鹿二仙胶含药血清诱导大鼠骨髓基质干细胞(BMSCs)成骨分化的作用并探讨其机制。方法全骨髓贴壁法分离BMSCs,培养至F3代。MTT法检测龟鹿二仙胶含药血清增殖作用并确定最佳浓度;F3细胞分为10%FBS组、10%KB组、10%GLEXJ组和Class...目的观察龟鹿二仙胶含药血清诱导大鼠骨髓基质干细胞(BMSCs)成骨分化的作用并探讨其机制。方法全骨髓贴壁法分离BMSCs,培养至F3代。MTT法检测龟鹿二仙胶含药血清增殖作用并确定最佳浓度;F3细胞分为10%FBS组、10%KB组、10%GLEXJ组和Classical组,检测碱性磷酸酶(Alkaline phosphatase,ALP)比活性,茜素红染色方法观察对BMSCs成骨分化的作用,RT-PCR方法和Western blot检测Fzd-2、Axin-2基因和蛋白表达的变化。结果 MTT结果显示浓度为10%的龟鹿二仙胶含药血清能促进BMSCs增殖;10%GLEXJ组和Classical组ALP比活性高于其余两组,差异有统计学意义(P<0.05);且随着时间的延长,增加更明显(P<0.05或P<0.01),茜素红染色见橘红色钙结节着色;10%GLEXJ组Fzd-2 m RNA和蛋白表达水平显著高于其余各组,差异均有统计学意义(P<0.05或P<0.01);10%GLEXJ组Axin-2 m RNA和蛋白表达水平显著低于10%FBS组和10%KB组,差异均有统计学意义(P<0.05或P<0.01)。结论龟鹿二仙胶含药血清能促进BMSCs增殖并诱导其向成骨细胞分化,其机制可能与增强Fzd-2表达、抑制Axin-2表达和激活Wnt/β-catenin通路密切相关。展开更多
基金National Natural Science Foundation of China(No.81973719)Science and technology research project of Liaoning Provincial Department of Education(No.L201921)Liaoning Provincial Science And Technology Plan Project(No.2020JH2/10300068)。
文摘Objective:This article uses the method of network pharmacology to study the related mechanism of Guilu Erxian Gum in the treatment of osteoporosis.Methods:Based on the TCMSP database,ETCM database,chemistry database,and DrugBank database,the potential active ingredients and related targets of Guilu Erxian Gum were obtained.The known therapeutic targets of osteoporosis were obtained from OMIM and Genecards databases,and the STRING database was used.A protein interaction network(PPI)of active ingredients-disease targets was established,and the topological parameters of the network were analyzed using Cytoscape 3.8.0 software to obtain key active ingredients and their targets.In R4.0.2,the Bioconductor data package was used to analyze the GO biological function and KEGG pathway analysis of key targets,and obtain the effective ingredients and targets of Guilu Erxian Gum for treating osteoporosis.Results:The prediction results show that Guilu Erxian Gum has 87 active ingredients and 2305 targets,and there are 4141 known therapeutic targets for osteoporosis.The two act together to obtain a total of 71 PPI core genes.The GO biological process analysis yielded 95 entries,and the KEGG pathway analysis yielded 115 pathways.Conclusion:The analysis results show that Guilu Erxian Gum may play an anti-osteoporosis effect by regulating inflammatory factors,promoting osteoblast differentiation,inhibiting osteoclast formation,and improving microcirculation.The pathways involved include TNF signaling pathways,IL-17 signaling pathway,NF-κB signaling pathway,HIF-1 signaling pathway,MAPK signaling pathway,PI3K-Akt signaling pathway,etc.This study provides a theoretical basis for further elucidating the pharmacological mechanism of Guilu Erxian Gum in the treatment of osteoporosis.
文摘目的观察龟鹿二仙胶含药血清诱导大鼠骨髓基质干细胞(BMSCs)成骨分化的作用并探讨其机制。方法全骨髓贴壁法分离BMSCs,培养至F3代。MTT法检测龟鹿二仙胶含药血清增殖作用并确定最佳浓度;F3细胞分为10%FBS组、10%KB组、10%GLEXJ组和Classical组,检测碱性磷酸酶(Alkaline phosphatase,ALP)比活性,茜素红染色方法观察对BMSCs成骨分化的作用,RT-PCR方法和Western blot检测Fzd-2、Axin-2基因和蛋白表达的变化。结果 MTT结果显示浓度为10%的龟鹿二仙胶含药血清能促进BMSCs增殖;10%GLEXJ组和Classical组ALP比活性高于其余两组,差异有统计学意义(P<0.05);且随着时间的延长,增加更明显(P<0.05或P<0.01),茜素红染色见橘红色钙结节着色;10%GLEXJ组Fzd-2 m RNA和蛋白表达水平显著高于其余各组,差异均有统计学意义(P<0.05或P<0.01);10%GLEXJ组Axin-2 m RNA和蛋白表达水平显著低于10%FBS组和10%KB组,差异均有统计学意义(P<0.05或P<0.01)。结论龟鹿二仙胶含药血清能促进BMSCs增殖并诱导其向成骨细胞分化,其机制可能与增强Fzd-2表达、抑制Axin-2表达和激活Wnt/β-catenin通路密切相关。
