Objective:This article uses the method of network pharmacology to study the related mechanism of Guilu Erxian Gum in the treatment of osteoporosis.Methods:Based on the TCMSP database,ETCM database,chemistry database,a...Objective:This article uses the method of network pharmacology to study the related mechanism of Guilu Erxian Gum in the treatment of osteoporosis.Methods:Based on the TCMSP database,ETCM database,chemistry database,and DrugBank database,the potential active ingredients and related targets of Guilu Erxian Gum were obtained.The known therapeutic targets of osteoporosis were obtained from OMIM and Genecards databases,and the STRING database was used.A protein interaction network(PPI)of active ingredients-disease targets was established,and the topological parameters of the network were analyzed using Cytoscape 3.8.0 software to obtain key active ingredients and their targets.In R4.0.2,the Bioconductor data package was used to analyze the GO biological function and KEGG pathway analysis of key targets,and obtain the effective ingredients and targets of Guilu Erxian Gum for treating osteoporosis.Results:The prediction results show that Guilu Erxian Gum has 87 active ingredients and 2305 targets,and there are 4141 known therapeutic targets for osteoporosis.The two act together to obtain a total of 71 PPI core genes.The GO biological process analysis yielded 95 entries,and the KEGG pathway analysis yielded 115 pathways.Conclusion:The analysis results show that Guilu Erxian Gum may play an anti-osteoporosis effect by regulating inflammatory factors,promoting osteoblast differentiation,inhibiting osteoclast formation,and improving microcirculation.The pathways involved include TNF signaling pathways,IL-17 signaling pathway,NF-κB signaling pathway,HIF-1 signaling pathway,MAPK signaling pathway,PI3K-Akt signaling pathway,etc.This study provides a theoretical basis for further elucidating the pharmacological mechanism of Guilu Erxian Gum in the treatment of osteoporosis.展开更多
[目的]探讨龟甲胶、鹿角胶等血肉有情之品治疗难治性再生障碍性贫血(refractory or resistant aplastic anemia,rAA)的运用规律,以期进行临床指导。[方法]通过整理中国中医科学院西苑医院血液科门诊所见的病例,并查阅相关文献和中医古籍...[目的]探讨龟甲胶、鹿角胶等血肉有情之品治疗难治性再生障碍性贫血(refractory or resistant aplastic anemia,rAA)的运用规律,以期进行临床指导。[方法]通过整理中国中医科学院西苑医院血液科门诊所见的病例,并查阅相关文献和中医古籍,学习血肉有情之品的药物特点以及临床运用原则,从病因、病机、治疗等方面探析血肉有情之品治疗rAA的理论依据,并举验案一隅加以证实。[结果]rAA患者临床疗效较差,患者长期输血依赖,感染和出血的风险较高,生存质量低下。唐师认为,rAA发病多因正气本虚、外邪侵袭所致,其根本病机是肾虚,病位在髓,治疗上运用血肉有情之品以补益肾精,同补肾之阴阳,使阴阳调和,促进气血恢复。在临床实践中,唐师采取中西医结合的治疗方式,以龟鹿二仙胶等血肉有情之品的中药为主,补益精血、养肾益髓,辅以少量西药,往往能取得良好的治疗效果。[结论]运用龟甲胶、鹿角胶等血肉有情之品,从其养血益精的本义出发,再结合患者症状,随证论之,对于改善rAA患者的症状效果颇佳。展开更多
目的观察龟鹿二仙胶干预阿尔茨海默病(AD)Virchow-Robin腔(VRS)及炎症的效应机制。方法80例西医诊断为AD,中医诊断为肾虚髓空证的患者随机分成两组,每组40例。两组患者都睡前口服安理申,每次1片(5mg),在此基础上对照组给予安慰剂,治疗...目的观察龟鹿二仙胶干预阿尔茨海默病(AD)Virchow-Robin腔(VRS)及炎症的效应机制。方法80例西医诊断为AD,中医诊断为肾虚髓空证的患者随机分成两组,每组40例。两组患者都睡前口服安理申,每次1片(5mg),在此基础上对照组给予安慰剂,治疗组采用龟鹿二仙胶治疗,疗程为60天。两组患者治疗前(第0天)、治疗后第31天及第61天分别进行头颅核磁共振成像(Nuclear Magnetic Resonance Imaging,MRI)检查,计数中脑、基底节区、岛叶深部白质、半卵圆中心VRS的数目,并测量其直径;此外,检测两组患者血清中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-1(IL-1)。结果两组患者各项指标在第31天时候皆无变化,差异无统计学意义(P>0.05);第61天,两组患者各部位VRS数目较各自第0天无变化(P>0.05),组间比较差异亦无统计学意义(P>0.05),而VRS直径、TNF-α、IL-6、IL-1,两组与治疗前比较皆有统计学差异(P<0.05,P<0.01),且组间差异亦有统计学意义(P<0.05,P<0.01)。结论龟鹿二仙胶具有改善患者VRS直径及调节炎症的作用,此可能为其补肾生髓的作用机制之一。展开更多
基金National Natural Science Foundation of China(No.81973719)Science and technology research project of Liaoning Provincial Department of Education(No.L201921)Liaoning Provincial Science And Technology Plan Project(No.2020JH2/10300068)。
文摘Objective:This article uses the method of network pharmacology to study the related mechanism of Guilu Erxian Gum in the treatment of osteoporosis.Methods:Based on the TCMSP database,ETCM database,chemistry database,and DrugBank database,the potential active ingredients and related targets of Guilu Erxian Gum were obtained.The known therapeutic targets of osteoporosis were obtained from OMIM and Genecards databases,and the STRING database was used.A protein interaction network(PPI)of active ingredients-disease targets was established,and the topological parameters of the network were analyzed using Cytoscape 3.8.0 software to obtain key active ingredients and their targets.In R4.0.2,the Bioconductor data package was used to analyze the GO biological function and KEGG pathway analysis of key targets,and obtain the effective ingredients and targets of Guilu Erxian Gum for treating osteoporosis.Results:The prediction results show that Guilu Erxian Gum has 87 active ingredients and 2305 targets,and there are 4141 known therapeutic targets for osteoporosis.The two act together to obtain a total of 71 PPI core genes.The GO biological process analysis yielded 95 entries,and the KEGG pathway analysis yielded 115 pathways.Conclusion:The analysis results show that Guilu Erxian Gum may play an anti-osteoporosis effect by regulating inflammatory factors,promoting osteoblast differentiation,inhibiting osteoclast formation,and improving microcirculation.The pathways involved include TNF signaling pathways,IL-17 signaling pathway,NF-κB signaling pathway,HIF-1 signaling pathway,MAPK signaling pathway,PI3K-Akt signaling pathway,etc.This study provides a theoretical basis for further elucidating the pharmacological mechanism of Guilu Erxian Gum in the treatment of osteoporosis.
文摘[目的]探讨龟甲胶、鹿角胶等血肉有情之品治疗难治性再生障碍性贫血(refractory or resistant aplastic anemia,rAA)的运用规律,以期进行临床指导。[方法]通过整理中国中医科学院西苑医院血液科门诊所见的病例,并查阅相关文献和中医古籍,学习血肉有情之品的药物特点以及临床运用原则,从病因、病机、治疗等方面探析血肉有情之品治疗rAA的理论依据,并举验案一隅加以证实。[结果]rAA患者临床疗效较差,患者长期输血依赖,感染和出血的风险较高,生存质量低下。唐师认为,rAA发病多因正气本虚、外邪侵袭所致,其根本病机是肾虚,病位在髓,治疗上运用血肉有情之品以补益肾精,同补肾之阴阳,使阴阳调和,促进气血恢复。在临床实践中,唐师采取中西医结合的治疗方式,以龟鹿二仙胶等血肉有情之品的中药为主,补益精血、养肾益髓,辅以少量西药,往往能取得良好的治疗效果。[结论]运用龟甲胶、鹿角胶等血肉有情之品,从其养血益精的本义出发,再结合患者症状,随证论之,对于改善rAA患者的症状效果颇佳。
文摘目的探究2种剂型龟鹿二仙胶对于腺嘌呤诱导肾阳虚型少弱精子症大鼠的干预作用。方法32只8周龄SD大鼠,体质量200 g-220 g,随机分4组,分别为空白组(Control)、模型组(Model)、龟鹿二仙胶饮片膏组(GLEXT)、龟鹿二仙胶颗粒膏组(GLEXG),每组8只;空白组灌胃等体积蒸馏水,其余4组给与腺嘌呤混悬液灌胃,连续灌胃30天;从31天起,空白组及模型组灌胃等体积蒸馏水,龟鹿二仙胶饮片膏组予龟鹿二仙胶饮片膏灌胃,龟鹿二仙胶颗粒膏组予龟鹿二仙胶颗粒膏灌胃,连续灌胃至60天。每日灌胃剂量10 m L·kg^(-1)。给药完毕后处死,检测血清性激素(T、E2、FSH、LH)、精液参数(精子活力、活率、密度)和睾丸HE染色实验。结果龟鹿二仙胶饮片膏组与龟鹿二仙胶颗粒膏组都能调节性激素分泌、改善大鼠精液参数(P<0.01,P<0.05),且在干预效果上无显著差异(P>0.05);龟鹿二仙胶饮片膏组与龟鹿二仙胶颗粒膏都对大鼠睾丸组织有保护作用。结论两种剂型龟鹿二仙胶对于腺嘌呤诱导肾阳虚型少弱精子症大鼠有干预作用且疗效相近,可能与其对下丘脑-垂体-睾丸轴的调控、性激素的改变、睾丸组织的保护有关。
文摘目的观察龟鹿二仙胶干预阿尔茨海默病(AD)Virchow-Robin腔(VRS)及炎症的效应机制。方法80例西医诊断为AD,中医诊断为肾虚髓空证的患者随机分成两组,每组40例。两组患者都睡前口服安理申,每次1片(5mg),在此基础上对照组给予安慰剂,治疗组采用龟鹿二仙胶治疗,疗程为60天。两组患者治疗前(第0天)、治疗后第31天及第61天分别进行头颅核磁共振成像(Nuclear Magnetic Resonance Imaging,MRI)检查,计数中脑、基底节区、岛叶深部白质、半卵圆中心VRS的数目,并测量其直径;此外,检测两组患者血清中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-1(IL-1)。结果两组患者各项指标在第31天时候皆无变化,差异无统计学意义(P>0.05);第61天,两组患者各部位VRS数目较各自第0天无变化(P>0.05),组间比较差异亦无统计学意义(P>0.05),而VRS直径、TNF-α、IL-6、IL-1,两组与治疗前比较皆有统计学差异(P<0.05,P<0.01),且组间差异亦有统计学意义(P<0.05,P<0.01)。结论龟鹿二仙胶具有改善患者VRS直径及调节炎症的作用,此可能为其补肾生髓的作用机制之一。
