In the present study, a gross quality control (QC) procedure is proposed for the Global Navigation Satellite System Occultation Sounder (GNOS) Global Positioning System radio occultation (GPS RO) refractivity data to ...In the present study, a gross quality control (QC) procedure is proposed for the Global Navigation Satellite System Occultation Sounder (GNOS) Global Positioning System radio occultation (GPS RO) refractivity data to remove abnormal data before they are assimilated. It consists of a climate extreme check removing data outside the range of the Constellation Observing System for Meteorology, Ionosphere and Climate (COSMIC) climate maxima and minima over approximately five years, and a vertical gradient check that rejects profiles containing super-refractions. These two QC steps were applied sequentially to identify outliers in GNOS GPS RO refractivity data during boreal winter 2013/2014.All of the abnormal refractivity profiles and the outliers at each level of the GNOS GPS RO observations were effectively removed by the proposed QC procedure. The post-QC GNOS GPS RO refractivity observations were then assimilated in the Global/Regional Analysis and PrEdiction System (GRAPES) using the three-dimensional variational(3D-Var) system. The impacts of the GNOS refractivity observation on GRAPES analysis and forecasting were evaluated and analyzed using an observation system experiment run over one whole winter season of 2013/2014. The experiment results demonstrated a positive impact of GNOS GPS RO data on analysis and forecast quality. The root mean squared error of GRAPES analysis temperature was reduced by 1%in the Southern Hemisphere (SH) extratropics and in the tropics, and the anomaly correlation scores of the forecasted 500-hPa geopotential height over the SH increased significantly during days 1 to 5. Overall, the benefits of using GNOS GPS RO data are significant in the SH and tropics.展开更多
The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the under...The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated berries.Our study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.展开更多
基金National Key R&D Program of China(2018YFC1506205,2018YFC1506702)。
文摘In the present study, a gross quality control (QC) procedure is proposed for the Global Navigation Satellite System Occultation Sounder (GNOS) Global Positioning System radio occultation (GPS RO) refractivity data to remove abnormal data before they are assimilated. It consists of a climate extreme check removing data outside the range of the Constellation Observing System for Meteorology, Ionosphere and Climate (COSMIC) climate maxima and minima over approximately five years, and a vertical gradient check that rejects profiles containing super-refractions. These two QC steps were applied sequentially to identify outliers in GNOS GPS RO refractivity data during boreal winter 2013/2014.All of the abnormal refractivity profiles and the outliers at each level of the GNOS GPS RO observations were effectively removed by the proposed QC procedure. The post-QC GNOS GPS RO refractivity observations were then assimilated in the Global/Regional Analysis and PrEdiction System (GRAPES) using the three-dimensional variational(3D-Var) system. The impacts of the GNOS refractivity observation on GRAPES analysis and forecasting were evaluated and analyzed using an observation system experiment run over one whole winter season of 2013/2014. The experiment results demonstrated a positive impact of GNOS GPS RO data on analysis and forecast quality. The root mean squared error of GRAPES analysis temperature was reduced by 1%in the Southern Hemisphere (SH) extratropics and in the tropics, and the anomaly correlation scores of the forecasted 500-hPa geopotential height over the SH increased significantly during days 1 to 5. Overall, the benefits of using GNOS GPS RO data are significant in the SH and tropics.
基金financially supported by the National Natural Science Foundation of China(32202560 and 32302470)the Program for Innovative Research Team(in Science and Technology)in University of Henan Province+6 种基金China(21IRTSTHN021)the Natural Science Foundation of HenanChina(232300421112)the Program for Science&Technology Innovation Talents in Universities of Henan ProvinceChina(21HASTIT035)the PhD Research Startup Foundation of Henan University of Science and TechnologyChina(13480068 and 13480067)。
文摘The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated berries.Our study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.
