Ramulus Cinnamomi extract attenuates neuroinflammatory responses via downregulating TLR4/MyD88 signaling pathway in BV2 cells

Ramulus Cinnamomi （RC）, a traditional Chinese herb, has been used to attenuate inflammatory responses. The purpose of this study was to investigate the effect of RC extract on lipopolysaccharide （LPS）-induced neuroinflammation in BV2 microglial cells and the underlying mechanisms involved. BV2 cells were incubated with normal medium （control group）, LPS, LPS plus 30 pg/mL RC extract, or LPS plus 100 pg/mL RC extract. The BV2 cell morphology was observed under an optical microscope and cell viability was detected by MTT assay. Nitric oxide level in BV2 cells was detected using Griess regents, and the levels of interleukin-6, interleukin-1 β, and tumor necrosis factor u in BV2 cells were determined by ELISA. The expression levels of cyclooxygenase-2, Toll-like receptor 4 and myeloid differentiation factor 88 proteins were detected by western blot assay. Compared with the LPS group, both 30 and 100 μg/mL RC extract had no significant effect on the viability of BV2 cells. The levels of nitric oxide, interleukin-6, interleukin-1β and tumor necrosis factor ct in BV2 cells were all significantly increased after LPS induction, and the levels were significantly reversed after treatment with 30 and 100 μg/mL RC extract. Furthermore, RC extract significantly inhibited the protein expression levels of cyclooxygenase-2, Toll-like receptor 4 and myeloid differentiation factor 88 in LPS-induced BV2 cells. Our findings suggest that RC extract alleviates neuroinflammation by downregulating the TLR4/MyD88 signaling pathway.

Chemical component analysis of volatile oil in drug pair Herba Ephedrae-Ramulus Cinnamomi by GC-MS and CRM

Active volatile components in drug pair(DP)Herba Ephedrae-Ramulus Cinnamomi(HE-RC),single drug HE and RC were analyzed by gas chromatography/mass spectrometry(GC/MS),chemometric resolution method(CRM)and overall volume integration.By means of CRM,the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound.In total,97,62,and 78 volatile chemical components in volatile oil of HE,RC,and DP HE-RC,were respectively determined qualitatively and quantitatively,accounting for 90.08%,91.62%,and 89.76% total contents of volatile oil of HE,RC,and DP HE-RC respectively.It is further demonstrated that the numbers of volatile components of DP HE-RC are almost the sum of those of two single drugs,but some relative contents of them are changed.Some new components,such as 1,6-dimethylhepta-1,3,5-triene,tetracyclo[4.2.1.1(2,5).0(9,10)]deca-3,7-diene,globulol and(E,E)-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one are found in DP HE-RC because of chemical reactions and physical changes during decoction.

Analysis of volatile components in herbal pair herba schizonepetae-ramulus cinnamomi

Analysis of volatile components in herbal pair (HP) herba schizonepetae-ramulus cinnamomi (HS-RC), single herb HS and RC was carried out by gas chromatography-mass spectrometry (GC-MS) data and chemometric resolution method (CRM). The two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound by CRM. In total, 47, 61 and 51 chemical components in volatile oil of HS, RC, and HP HS-RC were respectively determined qualitatively and quantitatively, accounting for 90.52%, 88.37%, and 88.72% total contents of volatile oil of HS, RC, and HP HS-RC, respectively. The number of the volatile components of HP HS-RC is almost the addition of that of two single herbs, but their relative contents are changed.

Mechanisms of Cinnamomi Ramulus for coronary heart disease treatment:prediction based on network pharmacology

Chinese medicine.However,little is known about the potential mechanism.Elucidating the effective components and mechanism based on network pharmacology was our purpose.Methods:Traditional Chinese Medicine Systems Pharmacology was screened to collect the possible active ingredients and their CAS and SMILES was searched in Pubchem,and the related potential targets were further predicted in Swiss Target Prediction database.Coronary heart disease molecular members were gained from GeenCards database,and the predicted targets of Cinnamomi Ramulus for coronary heart disease’s treatment were selected by Wayne diagram.As for mechanism analysis,String was used to construct the protein-protein interactions,and DAVID was used to conduct the GO and KEGG analysis.Results:Through GO and KEGG analysis,we found that cAMP signaling pathway,estrogen signaling pathway,calcium signaling pathway was related with coronary heart disease.Using network-based systems biology,we predicted that 10 active ingredients in Cinnamomi Ramulus have the treating effects with 78 potential targets.PIK3CG,MAPK8,BCL-2,BAX,PRKACA,CASP3,CALM1,CALM2,CALM3,NOS2,NOS3 were mainly involved in the treating effects of Cinnamomi Ramulus.Conclusion:Cinnamomi Ramulus may treat coronary heart disease through cAMP signaling pathway,estrogen signaling pathway,calcium signaling pathway.PIK3CG,MAPK8,BCL-2,BAX,PRKACA,CASP3,CALM1,CALM2,CALM3,NOS2,NOS3 were supposed to be considerable targets for treating coronary heart disease.

基于营卫理论探讨桂枝-赤芍配伍重塑肿瘤血管微环境的网络药理学机制

目的:采用网络药理学方法预测桂枝-赤芍配伍影响肿瘤血管生成的潜在靶点和通路,从分子网络药理学水平探索该配伍重塑肿瘤血管微环境的网络药理学机制。方法:采用中药系统药理学数据库与分析平台(TCMSP)检索桂枝、赤芍的化学成分和潜在靶点,选择口服生物利用度≥30%和类药性≥0.18作为化学成分筛选条件;在GeneCards数据库中检索血管生成的靶点;利用Cytoscape 3.6.0软件绘制桂枝-赤芍配伍-化合物-靶点-血管生成网络;使用STRING 11.0在线软件构建蛋白质-蛋白质相互作用(PPI)网络并挖掘核心靶点;采用David Bioinformatics Resources数据库对该复方活性成分潜在的靶点网络中的蛋白进行基因本体(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。结果:桂枝-赤芍配伍的33种有效成分作用于血管生成过程的77个靶点,PPI网络的核心靶点包括蛋白激酶B(Akt)1、JUN、白细胞介素6、基质金属蛋白酶、血管内皮生长因子A、一氧化氮合酶2和缺氧诱导因子-1α等多个蛋白。GO功能富集分析提示,该配伍的关键蛋白主要参与了DNA结合转录激活剂活性、血红素结合、抗氧化活性、核受体活性和转录因子活性等生物过程。KEGG通路富集分析显示,该配伍参与了缺氧诱导因子-1(HIF-1)信号通路、肿瘤蛋白53信号通路、细胞凋亡信号通路、表皮生长因子受体酪氨酸激酶抑制剂耐药信号通路、血管内皮生长因子(VEGF)信号通路和磷脂酰肌醇3激酶-Akt信号通路等,提示桂枝-赤芍配伍与肿瘤血管生成的关系最为密切。结论:桂枝-赤芍配伍中的黄芩素、谷甾醇和鞣花酸等成分可能通过HIF-1信号通路、VEGF信号通路影响肿瘤血管生成,干预肿瘤的生物学行为。

桂枝及桂枝类方治疗心血管疾病研究进展

桂枝是我国传统中药材,因其具有发汗解肌、平冲降逆、温经通脉、温阳助气等功效被广泛应用于医疗实践中,特别是在心血管疾病中尤为突出。现代研究发现桂枝及桂枝类方具有保护血管和调节血管活性因子、抗炎、抗氧化应激、改善冠状动脉循环和心肌纤维化、降血压、防治心力衰竭等作用,充分发挥对心血管疾病的防治作用。文章综述了桂枝及桂枝类方从抗冠心病、抗高血压、改善冠状动脉循环和心肌纤维化、调节血脂代谢、改善心肌缺血再灌注、防治心力衰竭等内容,以求为桂枝及桂枝类方在心血管疾病的应用和研究提供参考和依据。

经典名方中桂枝的关键信息考证与质量评价研究

目的通过本草考证和多批次道地产区桂枝药材的质量评价,为含桂枝的经典名方的研究提供依据与参考。方法对历代本草和经典医书进行考证,对经典名方中桂枝的药用部位、基原、产地与品质进行考证;并根据考证结果收集4个道地产地的15批次合格桂枝药材,建立适用于桂枝药材的高效液相色谱(HPLC)特征图谱方法并进行方法学验证;通过聚类分析、主成分分析和偏最小二乘-判别分析方法,结合特征图谱数据和桂皮醛含量,对古籍考证的道地产区桂枝药材的质量一致性进行综合评价。结果通过古籍考证,确定了经典名方中所用桂枝的药用部位(嫩枝)、基原(樟科植物肉桂)、产地(广东、广西);建立了通过方法学验证的HPLC特征图谱方法,形成的共有对照特征图谱共计10个共有特征峰,并指认出香豆素、肉桂酸、桂皮醛以及2-甲氧基肉桂醛4个色谱峰;3个化学识别分析结果均表明古籍考证的道地产区桂枝药材质量具有一致性。结论经典名方中桂枝选用樟科植物肉桂(Cinnamomum cassia Presl)的干燥嫩枝,产地可选用广西和广东两地;建立的桂枝特征图谱方法适用于桂枝的质量控制研究。

麻黄-桂枝药对治疗呼吸系统疾病的临床应用

麻黄是指麻黄科的草麻黄、中麻黄或木贼麻黄的草质茎部,桂枝是樟科植株肉桂的嫩枝。麻黄与桂枝均为解表中药,二者属于相须配伍。目前关于麻黄-桂枝药对的研究多集中于二药配伍的化学成分及作用机制,关于麻黄-桂枝药对的临床应用研究尚且较少,现将通过研究总结近年来麻黄、桂枝的相关文献,对麻黄与桂枝的药理作用、麻黄与桂枝的炮制方法与用量、麻黄与桂枝配伍的作用、含麻黄-桂枝药对的方剂在呼吸系统疾病中的临床应用这4个方面进行综述。

黄芪桂枝五物汤加减对肩周炎患者肩关节功能、炎性因子和血清5-HT、PGE2的影响

目的探讨黄芪桂枝五物汤加减对肩周炎患者肩关节功能、炎性因子和血清5-HT、PGE2的影响。方法选取2022年2月—2023年2月西安市阎良区人民医院收治的80例肩周炎患者作为研究对象,应用随机数表法将其分为观察组与对照组,每组40例。对照组采取常规治疗,观察组在对照组治疗基础上联合黄芪桂枝五物汤加减治疗,应用Constant-Murley评分及视觉模拟量表(Visual simulation scale,VAS)评价患者治疗前后的肩关节功能变化,并比较其炎性因子水平及血清中5-羟色胺(5-hydroxytryptamine,5-HT)和前列腺素E2(Prostaglandin E2,PGE2)表达水平。结果治疗前两组患者肩关节功能评分、疼痛评分比较,差异无统计学意义(P>0.05),治疗后两组患者肩关节功能评分均升高,且观察组高于对照组,差异有统计学意义(P<0.05);疼痛评分均降低,且观察组疼痛评分低于对照组,差异有统计学意义(P<0.05);治疗前两组患者肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、转化生长因子-β1(Transforming growth factor-βone,TGF-β1)、白细胞计数(White blood cell count,WBC)、C反应蛋白(C-reactive protein,CRP)水平比较,差异无统计学意义(P>0.05),治疗后两组患者TNF-α、TGF-β1、WBC、CRP水平均降低,且观察组低于对照组,差异有统计学意义(P<0.05);治疗前两组患者5-HT、PGE2比较,差异无统计学意义(P>0.05),治疗后两组患者5-HT、PGE2均降低,且观察组低于对照组,差异有统计学意义(P<0.05)。结论对肩周炎患者在常规治疗基础上联合黄芪桂枝五物汤加减治疗可改善患者肩关节功能,降低疼痛程度,减轻机体炎性反应水平,其治疗机制可能与血清5-HT、PGE2的降低具有一定关系,值得临床应用。

汤小虎“桂枝主气”辨治风湿免疫疾病学术思想及临床运用

归纳整理云南省“云岭名医”汤小虎教授“桂枝主气”学术思想,及运用桂枝及其类方治疗风湿免疫疾病经验。“桂枝主气”理论,即“桂枝能助一身之气用”是汤小虎教授治疗“痹证”的重要学术思想。汤小虎教授治疗“痹证”主要临证治疗特点:提倡以桂枝“温扶阳气”,治疗过程中应当维持“少火生气”,避免“壮火食气”之弊;强调桂枝及其类方在治疗中当依据风、寒、湿、热等灵活辨证施治;重视桂枝的方药配伍,如“桂枝-附子”“桂枝-黄芪”“桂枝-甘草”“桂枝-麻黄”“桂枝-芍药”合用体现不同功效;重视特色用药及道地药材配伍协同增强疗效;强调“达标治疗策略及因人制宜”,包括早期诊断、早期强化治疗、长程管理、严格控制、精确疾病活动评价体系建立和个体化治疗。运用汤小虎教授“桂枝主气”学术思想指导桂枝及其类方治疗风湿免疫疾病,临床疗效好,值得学习与推广运用。

桂枝的化学成分和药理作用研究进展

桂枝为樟科植物肉桂的干燥嫩枝,是临床上使用频率最高的中草药之一。其化学成分主要包含挥发性成分、有机酸类、糖苷类等,主要有调节体温、镇痛、抑菌、抗炎、抗过敏、抗病毒、促进血管舒张、利尿、镇静、抗焦虑、抗肿瘤、降血压等药理作用。现对桂枝的化学成分及药理作用进行综述,以期为进一步研究桂枝以及对其药理作用的应用提供理论依据和参考价值。

从《神农本草经》探析《伤寒杂病论》中桂枝的用药特点

以《神农本草经》所载“味辛,温。主上气咳逆,结气喉痹,吐吸,利关节,补中益气”为基础,分析《伤寒杂病论》中桂枝的用药特点,认为桂枝在《伤寒杂病论》同一方中常表现为一药多用,组方精妙,仲景在《神农本草经》的基础上对桂枝的应用多有创新发挥。故在临床中使用桂枝宜兼顾其性味、功效及配伍,并结合实际情况灵活施治,切不可局限于一药治一证。

基于网络药理学分析苍术-桂枝治疗非酒精性脂肪性肝炎的作用机制

目的网络药理学筛选苍术-桂枝治疗非酒精性脂肪性肝炎的有效成分和靶点。方法应用TCMSP和Swiss查找苍术、桂枝的活性成分的靶点,通过OMIM和GeneCards数据检索非酒精性脂肪性肝炎的靶点,获取交集靶点,利用Cytoscape 3.6.0构建苍术-桂枝与非酒精性脂肪性肝炎的“药物-成分-疾病-靶点”的网络图,筛选出关键靶点,进行GO和KEGG富集分析,最后利用分子对接验证关键靶点。结果得到苍术-桂枝有效活性成分16个,交集靶点77个,蛋白互作分析(PPI)得到ACE、APOB、CAT、TP53、EGFR、PPARG等16个核心靶点。KEGG和GO结果显示:主要影响脂质颗粒、细胞外间隙、内质网等10种细胞成分,脂肪酸结合、肝素结合、脂质转运体活性等10种分子功能,作用于炎症反应、胆固醇稳态、胆固醇代谢过程等10种生物过程,主要通过脂质与动脉粥样硬化、胰岛素抵抗、非酒精性脂肪肝等13种通路对非酒精性脂肪性肝炎起作用,分子对接结果证明,16中有效成分与靶点具有较强的结合能。结论苍术-桂枝改善非酒精性脂肪性肝炎的作用机制可能通过胰岛素抵抗、非酒精性脂肪肝等通路实现的,具有多个靶点,多个成分,多条通路的特点。

基于网络药理学与分子对接探究桂枝—甘草治疗病态窦房结综合征的药理机制

目的基于网络药理学探究桂枝—甘草治疗病态窦房结综合征(sick sinus syndrome,SSS)的药理机制,为今后新药成分开发及规范指导临床提供科学依据。方法检索TCMSP数据库获取桂枝—甘草的活性成分及相关靶点,利用Uniprot数据库进行基因标准化,进而构建中药—活性成分—基因网络。通过GeneCards、OMIM、DisGeNET数据库检索SSS的疾病靶点,利用韦恩图获取交集靶点,借助STRING数据库及Cytoscape3.7.2构建蛋白互作(protein-protein interaction,PPI)网络模型,获取关键靶点,通过Metascape网站及微生信在线构图进行基因本体(gene ontology,GO)功能富集分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,利用AutoDock对核心靶点进行分子对接验证。结果收集并筛选桂枝—甘草核心成分为槲皮素、山柰酚、β-谷甾醇、柚皮素等;PPI结果显示白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子(tumor necrosis factor,TNF)、蛋白激酶B(protein kinase B,AKT1)、白细胞介素-1β(interleukin-1β,IL-1β)、血管内皮细胞生长因子(vascular endothelial growth factor-A,VEGFA)关系作用最强;GO分析选取主要富集条目1686条,细胞组成1511条,分子功能65条,主要生物学过程为110条,KEGG富集主要通路涉及脂质和动脉粥样硬化通路、坏死性凋亡、钙信号通路、NF-κB信号通路、病毒性心肌炎、糖尿病性心肌病等;分子对接显示核心蛋白配体与核心成分受体能稳定对接。结论桂枝—甘草药对可能通过其调控心肌细胞凋亡、延缓窦房结(sinoatrial node,SAN)纤维化、抑制炎症反应、改善内皮功能等生物过程治疗SSS。

Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study

Objective： To investigate the antipyretic mechanism of Herba Ephedrae （Eph）-Ramulus Cinnamomi （RC） herb pair on yeast-induced pyrexia in rats. Methods： Totally 30 qualified male SD rats were randomly assigned to the normal control （NC） group, the pyrexia model （model） group, the Eph, RC and Eph-RC treatment groups by a random digital table, 6 rats in each group. Each rat received a 20% aqueous suspension of yeast （10 mL/kg） except the NC group. The 3 treatment groups were administered 8.1, 5.4 and 13.5 g/kg Eph, RC and Eph-RC respectively at 5 and 12 h after yeast injection, the NC group and the model groups were administered equal volume of distilled water. Rectal temperatures were measured at 0, 6, 8, 10, 12, 15, 18, 24 and 30 h and urine was collected prior to yeast injection and at 6, 10, 18, 24, 30, and 36 h after yeast injection. Then urine metabolomic profiling by gas chromatography tandem mass spectrometry, coupled with multivariate statistical analysis and pattern recognition techniques were used to explore the antipyretic effects of Eph-RC. Partial least squares discriminate analysis was used to analyze the metabolomics dataset including classification and regression in metabolomics plot profiling. Results： Compared with the NC group, rectal temperatures were significantly higher in the model group （P〈0.01）, while 3 treatment groups decreased significantly compared with the model group （P〈0.05 or P〈0.01）. Rectal temperatures of Eph-RC-treated rats started to go down at 6 h, and markedly decreased at 8, 12, 15, 18 and 24 h （P〈0.05 or P〈0.01）, while those of the Eph and RC groups had decreased firstly at 8 h and were markedly lower at 12 h （P〈0.05 or P〈0.01）. Seventeen potential biomarkers related to pyrexia were confirmed and identified, including pyruvic acid, L-phenylalanine, L-tyrosine, phenylacetic acid, hippuric acid, succinic acid, citrate and so on. Eight potential alterations of metabolic pathways including phenylalanine metabolism, citrate cycle, tryptophan metabolism, biosynthesis of valine, leucine and isoleucine, were identified in relation to the antipyretic effects of Eph-RC using MetPA software. Conclusion： The antipyretic effect of Eph-RC herb pair on yeast-induced pyrexia in rats involved correction of perturbed amino acid, fatty acid, and carbohydrate metabolism according to the metabolic pathway analysis with MetPA.

从胃强脾弱探讨小儿营养不良的治疗

小儿营养不良可归属中医学的“疳证”范畴。临床中部分营养不良患儿可见多食易饥、形体消瘦、大便不调等主症,符合“胃强脾弱”证型表现,此类患儿的治疗可采用运脾泻热、调和营卫之法,处方用药可予益黄散(主要由陈皮、诃子、青皮、甘草等组成)合泻黄散(主要由防风、藿香、石膏、栀子、甘草等组成)加桂枝、白芍、苍术,以实现整体调理脏腑功能,治疗营养不良的目的。

Effect of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi Compatibility on Uric Acid Metabolism and Urinary Neutrophil Gelatinase-Associated Lipocalin and Kidney Injury Molecule-1 in Rats with Hyperuricemia

Objective：To explore the effects of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomicompatibility（PR） on uric acid metabolism and the expression of urinary neutrophil gelatinase-associated lipocalin（NGAL） and kidney injury molecule-1（KIM-1） in rats with hyperuricemia. Methods：Seventy male Sprague Dawley（SD） rats were randomly divided into 7 groups with 10 rats per group, including the normal group, model group, allopurinol group, benzbromarone group and PR groups at 3 doses（3.5, 7, 14 g/kg）. Except the normal group, rats of the other groups were intragastrically administered 100 mg/kg hypoxanthine and 250 mg/kg ethambutol, and subcutaneously injected with 200 mg/kg potassium oxonate. All rats were continuously modeled for 17 days, and gavaged with corresponding drugs. The rats of the normal and model groups were gavaged with saline, once a day, for 2 weeks. The levels of serum uric acid（SUA）, blood urea nitrogen（BUN） and creatinine（Cr） were determined. In addition, the contents of NGAL and KIM-1 in urine and the m RNA and protein expressions of xanthine oxidase（XOD） in liver of hyperuricemia rats were measured by reverse transcription polymerase chain reaction（RT-PCR） and Western blot, respectively. Moreover, the pathological changes of kidney were analyzed by hematoxylin and eosin（HE） stain method. Results：Compared with the normal group, the levels of SUA, BUN, NGAL and KIM-1 and the expressions of hepatic XOD m RNA and protein in the hyperuricemia rats were increased significantly（P〈0.01）. PR significantly decreased the levels of SUA, BUN, NGAL and KIM-1 and down-regulated the m RNA and protein expressions of hepatic XOD（P〈0.05 or P〈0.01）. In addition, the pathological changes of kidney were significantly suppressed by oral administration of PR. Conclusions：PR ameliorated uric acid metabolism and protected renal function, the underlying mechanism was mediated by decreasing the levels of SUA, BUN, NGAL and KIM-1, inhibiting the expression of hepatic XOD and ameliorating the pathological change of kidney.

Advances in Research of Epidemic Lung Preventive Mixture

This paper summarizes the Epidemic Lung Preventive Mixture from three aspects of research status, development trends and prospects, so as to deepen the understanding of Epidemic Lung Preventive Mixture and provide a more detailed theoretical basis for its further clinical research and development.

枳实薤白桂枝汤中“桂枝”考证

桂枝作用于人体的部位偏上焦,肉桂则更偏于下焦,枳实薤白桂枝汤是用于治疗胸痹的名方,病位在上焦,故选桂枝较为恰当,若兼见下焦阳虚病证时,可酌情选用肉桂。枳实薤白桂枝汤中桂枝的功效为引枳实、厚朴入上焦以平冲逆,合瓜蒌以振胸阳,合薤白以散结气。历代医家受瓜蒌薤白白酒汤、瓜蒌薤白半夏汤二方病机的影响,皆以胸阳不振、痰浊上凝作为枳实薤白桂枝汤证的病机,但该证以胸满气结、气逆抢心为主,痰浊之象并不明显,故其病机应为阳虚气逆。药物煎煮方法中,桂枝与瓜蒌、薤白皆是轻煎,意在取其气味,而非重其功效,盖枳实、厚朴虽有下气降逆之力,却无入上焦之能,故须以桂枝轻清之气为引;上焦阳虚,无力镇摄阴霾之气上冲,故须桂枝、瓜蒌辛甘化阳,使阳气得充、胸阳得振;气结不去,胸中痞满难除,故须桂枝、薤白辛温相须,使结气得散。本方以桂枝为使,则逆者得降;辛甘相合,则虚者得补;辛温相须,则结者得散,体现了张仲景严谨的用药思路,也凸显了其煎煮方式的灵活性及对方药功效的影响。

Preventive effect of different compatibilities of Ramulus Cinnamomai and Radix Paeomiae alba in Guizhi decoction on cardiac sympathetic denervation induced by 6-OHDA

Objective To observe the preventive effect of different compatibilities of Ramulus Cinnamomi(RC)and Radix Paeomiae alba(RPA)in Guizhi Decoction(GZD)on neurotransmitters and their rate-limiting enzymes,and neurotrophic factors of cardiac sympathetic denervation model rats induced by 6-hydroxydopamine(6-OHDA).Methods Totally 54 male Wistar rats were randomly divided into 6 groups,i.e.,the blank control group。